• BMB Reports
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• 제40권1호
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• pp.22-28
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• 2007

MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

• Journal of Ginseng Research
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• 제19권1호
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• pp.56-61
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• 1995

Molecular cloning and restriction mapping on ATPase $\alpha$-subunit gene (atpA) were carried out to obtain genomic information concerned with the gene structure and organization in Korean ginseng mitochondria. Two different clones containing the homologous sequence of atpA gene were selected from SalI and PstI libraries of mitochondrial DNA (mtDNA) of Korean ginseng. The sizes of mtDNA fragments inserted in SalI and PstI clones were 3.4 kb and 13 kb, respectively. Southern blot analysis with [$^{32}P$] labelled Oenothera atPA gene probe showed that atpA gene sequence was located in 2.0 kb XkaI fragment in PstI clone and in 1.7 kb XbaI fragment in SalI clone. A partial sequening ascertained that the SalI clone included about 1.2 kb fragment from SalI restriction site to C-terminal sequence of this gene but about 0.3 kb N-terminal sequence of open reading frame was abscent. The PstI fragment was enough large to cover the full sequence of atpA gene. The same restriction pattern of the overlapped region suggests that both clones include the same fragment of atiA locus. Data of Southern blot analysis and partial nucleotide sequencing suggested that mtDNA of Korean ginseng has a single copy of atpA gene. Key words ATPase a-subunit, mitochondrial DNA, Panax ginseng.

• 미생물학회지
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• 제33권2호
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• pp.92-96
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• 1997

Pseudomonas sp. strain DJ77로부터 클로닝한 catechol 분해와 관련된 phnDEFG 유전자들이 존재하는 pHENX7에서 phnF 유전자의 염기서열을 밝혔다. Extradiol dioxygenase 유전자인 phnE와, 2-hydroxymuconic semialdehyde dehydrogenase를 생산하는 phnG 유전자 사이에 존재하는 유전자 phnF는 432 bps로 된 하나의 open reading frame(ORF)으로 존재하였고, 여기서 유추한 아미노산은 143개로 분자량 13,859 dalton의 polypeptide를 만들어 내고 있다. phnF 유전자는 Sphingomonas sp. strain HV3 catE 유전자 부위와 sphingomonas yanoikuyae B1의 xylE와 xylG 사이에 존재하는 ORF 부위의 염기서열과 각각 99%, 68.6%의 상동성을 가지고 있었다. 또한 PhnF 단백질의 아미노산서열은 citrobacter freundii DSM30040의 orfY 부위의 아미노산서열과 62.3%의 상동성이 있었다.

• 한국산학기술학회논문지
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• 제9권6호
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• pp.1619-1624
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• 2008

현재 유비쿼터스 서비스를 제공하기 위해 GPS(Global Positioning System), RFID(Radio Frequency IDentification) 및 센서 네트워크를 이용한 개별 미들웨어 및 인터페이스를 개발하고 있다. GPS는 주로 위치기반 서비스에, RFID를 식별을 통한 유통 물류 서비스에, 센서 노드는 온도, 습도, 기압 등의 상황 정보 수집에 응용되고 있다. 최근에 2 또는 3개의 이들 요소들이 농축산, 건설, 의료 등의 다양한 분야에 응용하면서 GPS, RFID 및 센서 노드를 통합하는 인터페이스 요구가 증가하고 있다. 이에 본 논문에서는 RFID, GPS, 센서 네트워크에서 수집된 상황데이터를 통합 처리하는 공통 인터페이스를 구현하고 실험을 통해 동작을 확인한다. 이 인터페이스는 필터링, 파싱, 인터페이스 관리, 큐잉, 하드웨어 및 미들웨어 접근 기능 등의 효율적인 데이터 처리 기능을 구현하고 동작을 검증한다. 그리고 GPS, RFID 및 센서 노드를 수집된 상황 데이터를 결합한 통합 프레임 구조를 제시하고, 복합형 유비쿼터스 응용 시스템에 제공되는 개방형 인터페이스를 제공하는 것을 확인한다. 이를 통하여 RFID, GPS, 센서 네트워크를 통해 얻어진 상황 데이터도 효과적으로 처리하여 다양한 유비쿼터스 응용 서비스에게 제공할 수 있을 것으로 사료된다.

• 한국산학기술학회논문지
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• 제12권2호
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• pp.959-969
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• 2011

본 연구는 임대주택의 구조방식이 벽식구조에서 라멘구조로 변화하는 시점에서 서울시 임대주택의 필로티공간계획의 주안점을 모색하고자 하였다. 이를 위해 서울시 임대주택의 현황을 분석하고 연구대상으로 선정된 7개 단지 28개동에 대해서 물리적, 공간적, 환경적 분석 및 사용자 면담을 실시하였다. 이를 통해 벽식구조 필로티 공간의 문제점을 인식하고 라멘구조로 전환시에 계획의 주안점을 다음과 같이 제시하였다. 첫째, 필로티 공간의 유효면적 증가와 쾌적성 향상에 따른 공간 활용의 다양성에 대응하기 위한 계획적 접근이 필요하다. 둘째, 필로티 공간의 특성상 다양한 기능이 복합되는 경우 기능의 경계를 명확히 구분하여 간섭과 충돌을 최소화하고 기능이 불명확한 공간으로 방치되지 않도록 계획하여야 한다. 셋째, 실내공간으로 전용가능성을 고려해야 한다. 상대적으로 외부공간이 부족한 임대주택에서 필로티 공간을 적극적으로 활용해야 하며 거주자의 다양한 요구를 수용하기 위해 이를 고려한 구조체배치 등의 계획적 접근이 요구된다. 넷째, 안전성을 확보해야 한다. 필로티 공간과 외부공간과의 시각적 연결관계를 고려해서 필로티 공간이 외부로부터 고립되지 않도록 계획하여야 한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.187-191
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• 2004

Actin is a ubiquitous and highly conserved protein found in eukaryotic organisms. In this study, we describe the cDNA cloning and mRNA expression of an actin gene from the mulberry longicorn beetle, Apriona germari. The A. germari actin cDNA is 1524 bp containing a complete 1128 bp open reading frame that encodes a polypeptide of 376 amino acid residues with a predicted molecular weight of about 41.5 kDa. The deduced amino acid sequence of the A.germari actin cDNA showed 99% protein sequence identity to Homalodisca coagulata actin, differing at only two amino acid positions, and 92-98% protein sequence identity to known insect species actins. The predicted three-dimensional structure of A. germari actin revealed the four residue hydrophobic pulg loop characteristic of the actin family. Northern blot analysis showed that A. germari actin is highly expressed in epidermis and muscle, and less strongly in midgut, but not in the fat body of A. germari larva.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.217-223
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• 2004

A cathepsin L-like cysteine protease, v-cath, encoded by the baculovirus has been shown to playa role in host liquefaction. We have identified a v-cath gene in the silkworm virus, Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 969 bp v-cath has an open reading frame of 323 amino acids. A putative cleavage site and catalytic sites were conserved in BmNPV-K1 v-cath. The predicted three-dimensional structure of BmNPV-K1 v-cath revealed that the overall fold of BmNPV-K1 v-cath is similar to that of other proteases of the papain family. The deduced amino acid sequence of BmNPV-K1 v-cath showed 98% and 97% protein sequence identity to BmNPV T3 strain and to Autographa californica nuclear polyhedrosis virus, respectively. The BmNPV-K1 v-cath differed at 4 amino acid positions from BmNPV T3. The v-cath gene in BmNPV-K1 genome is located on the EcoRV 6 kb and XhoI 9 kb fragments. Northern hybridization analysis of BmNPV K1 v-cath gene revealed that it is expressed late in infection.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권10호
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• pp.4002-4014
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• 2015

ITU-R M.1842-1, as a well-known specification dedicated to maritime mobile applications, has standardized wireless transmission protocols according to the particular characteristics of a maritime communications scenario. A time division multiple access (TDMA) frame structure, along with modulation schemes to achieve a high data rate, has been described clearly in ITU-R M.1842-1. However, several specification items are still under "to be decided" status, which brings ambiguity to research works. In addition, the current version of ITU-R M.1842-1 is focused mainly on maritime transmissions in open-sea areas, where the cyclic prefix (CP) is set to zero and only 16-QAM is used in the multi-carrier (MC) system. System performance might be dramatically degraded in coastline areas due to the inter-symbol interference (ISI) caused by selective fading. This is because there is a higher probability that the signal will be reflected by obstacles in coastline areas. In this paper, we introduce the transmission resource block (TRB) dedicated to ITU-R M.1842-1 for a ship ad-hoc network (SANET), where the pilot pattern of TRB is based on the terrestrial trunked radio (TETRA). After that, we evaluated SANET performance under the maritime channel model in a coastline area. In order to avoid noise amplification and to overcome the ISI caused by selective fading, several strategies are suggested and compared in the channel estimation and equalization procedures, where the link-level simulation results finally validate our proposals.

• 한국조경학회지
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• 제34권2호
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• pp.80-98
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• 2006

This study originated from following questions. What can we understand the conception of deconstruction, which has been the core idea of new discourses developed in various ways since modernism? How can this question be interpreted in landscape design? What is the conceptional frame of integration the prominent hybrid post-genre movements and phenomena? The frame can be epitomized with the deconstruction phenomenon. 'Deconstruction' is the core conception appeared in late or post-modern ages in the embodiment of modernity and can be viewed as an integrating or a hybrid phenomenon between areas or genres in formative arts. Therefore, the author regards the hybrid movements widely witnessed in the post contemporary formative arts as one of the most important indicators of de-constructive signs. It is safe to say that the phenomenon of this integration or hybridism, of course, does not threaten the identity of landscape design but serves as an opportunity to extend the areas of landscape design. One of the consequences of this integration or hybridism is the voluntary participation of users who have been alienated in the production of the meanings of design works and hybrid landscape design with the hybridization of genres that is characterized with transformation in forms. This view is based on the distinction between hybridization of interactions between the designer (the subject) and the user (the object), and hybridization of synesthesia. Generally speaking, this is an act of destroying boundaries of the daily life and arts. At the same time, it corresponds to vanishing of modern aesthetics and emerging of post-contemporary aesthetics which is a new aesthetic category like sublimeness. This types of landscape design tries to restore humans' sensibility and perceptions restrained by rationality and recognition in previous approach and to express non-materialistic characteristics with precaution against excessive materialism in the modern era. In light of these backgrounds, the study aims to suggest the hybrid concept and to explorer a new landscape design approach with this concept, in order to change the design structure from 'completed' or 'closed' toward 'opened' and to understand the characteristics of interactions between users and designs. This new approach is expected to create an open-space integrating complexity and dynamics of users. At the same time, it emphasizes senses of user' body with synesthesia and non-determination. The focus is placed on user participation and sublimity rather than on aesthetic beauty, which kind of experience is called simulacre. By attaching importance to user participation, the work got free from the material characteristics, and acceptance from the old practice of simple perception and contemplation. The boundaries between the subject and object and the beautiful and ordinary, from the perspective of this approach, are vanished. Now everything ordinary can become an artistic work. Western dichotomy and discrimination is not effective any more. And there is 'de-construction' where there is perfect equality between ordinary daily life and beautiful arts. Thus today's landscape design pays attention to the user and uses newly perceived sensitivity by pursing obscure and unfamiliar things rather than aesthetic beauty. Space is accordingly defined to take place accidentally as happening and event, not as volume of shape. It's the true way to express spatiality of landscape design. That's an attempt to reject conventional concepts about forms and space, which served as the basis for landscape design, and to search for new things.

• BMB Reports
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• 제39권4호
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• pp.346-354
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• 2006

The full-length cDNA of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) uncoupling protein 2 (UCP2) was obtained from liver. The grass carp UCP2 cDNA was determined to be 1152 bp in length with an open reading frame that encodes 310 amino acids. Five introns (Intron 3, 4, 5, 6 and 7) in the translated region, and partial sequence of Intron 2 in the untranslated region of grass carp UCP2 gene were also obtained. Gene structure comparison between grass carp and mammalian (human and mouse) UCP2 gene shows that, the UCP2 gene structure of grass carp is much similar to that of human and mouse. Partial UCP2 cDNA sequences of bighead carp (Aristichthys nobilis) and mud carp (Cirrhinus molitorella), were further determined. Together with the common carp (Cyprinus carpio) UCP2 sequence from GenBank (AJ243486), multiple alignment result shows that the nucleotide and amino acid sequences of the UCP2 gene, were highly conserved among the five major Chinese carps that belong to four subfamilies. Using beta-actin as control, the ratio UCP2/beta-actin mRNA (%) was determined to be $149.4{\pm}15.6$ (common carp), $127.4{\pm}22.1$ (mud carp), $96.7{\pm}12.7$ (silver carp), $94.1{\pm}26.8$ (bighead carp) and $63.7{\pm}16.2$ (grass carp). The relative liver UCP2 expression of the five major Chinese carps, shows a close relationship with their food habit: benthos and detrituseating fish (common carp and mud carp) > planktivorious fish (silver carp and bighead carp) > herbivorious fish (grass carp). We suggest that liver UCP2 might be important for Chinese carps to detoxify cyanotoxins and bacteria in debris and plankton food.