• International Journal of Industrial Entomology and Biomaterials
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• 제14권1호
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• pp.37-44
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• 2007

A study was made of the haemolymph protein spectrum of mulberry silkworm (Bomhyx mori L.) from the first larval instar to imago. Horizontal starch gel electrophoresis was used. Sixteen races and eight F1 interracial hybrids, raised in Bulgaria, were analyzed. During the ontogenesis, a total of 17 protein bands (15 cathodic and 2 anodic) were detected. Distinct dynamics in the haemolymph protein spectrum was observed, in result of different expression during the individual development associated with the processes of growth, histolysis and histogenesis. Based on the ontogenetic dynamics found, a correspondence was assumed between some proteins detected by us using the starch gel electrophoresis and major haemolymph proteins (SP1, SP2, MHPs and Vg) detected by other authors using the polyacrilamide gel electrophoresis. Intraracial and interracial polymorphism was observed in four protein zones. The effect of four polymorphic loci with codominant and null alleles was suggested.

• 한국유기농업학회지
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• 제3권1호
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• pp.11-22
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• 1994

Exposing plant organs to high doses of ionizing irradiation, penetrating into the plant tis-sues and cells, along the track structure of particles, lesions, and sublesions are formed on the molecules and organelles. As a result, disorders in the growth and development as well as chlorophyll-deficiency symptoms occur. The time scale of their reparation, recovery and over compensation during ontogenesis, constitutes a question of high theoretical and practical importanced, with special regard to nuclear fallout. With an aim to model the “ut supra”stated phenomena, the seeds of bean, Echo elit licensed variety, were irradiated by 300 Gy dose of X-ray-irradiation (120 kV:4.5 mA). According to the data obtained, the biosynthesis of photosynthetic pigments, will have been completed by the beginning of flowering. In consequence of the overcompensation of the repairing processes, the organs of plants developed from irradiated seeds, showed a partly differing correlative growth, compared to those of control plants. In order to characterize the vivo response of radiation-injured plants, a new method and approach were used. The changes of the electric capacitance of the plants during their ontogenesis, were continously monitored and recorede via a computer-aided and controlled measurement. In view of the data collected in such a way, the repairing plants may respond more quickly and intensively to the changes of environmental factors.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 1998년도 제4차 학술발표대회 및 정기총회
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• pp.29-29
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• 1998

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.59-63
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• 2004

By using PAGE, a study was made on the nonspecific esterase spectra of female genital organs and eggs in Bombyx mori L. The expression of 11 esterase bands was detected during ontogenesis of races and inter-races hybrids kept in Bulgaria. The gene activity of 9 esterase loci was assumed. Esterases specific for the spectrum of diapausing eggs were observed. In two esterase zones, intra- and inter-breed polymorphism was found. Based on the same breed specific expression, the existence of correspondence between esterase bands from spectra of different silkworm tissues and organs was suggested. Stage-specific expression of esterases in female genital glands, indicative of differentiated gene activity during ontogenesis, was established.

• Molecules and Cells
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• 제38권11호
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• pp.959-965
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• 2015

Inducible and reversible gene silencing in desired types of cells is instrumental for deciphering gene functions using cultured cells or in vivo models. However, efficient conditional gene knockdown systems remain to be established. Here, we report the generation of an inducible expression system for short hairpin RNA (shRNA) targeted to PTEN, a well-documented dual-specificity phosphatase involved in tumor suppression and ontogenesis. Upon induction by doxycycline (DOX), the reverse tetracycline transcriptional activator (rtTA) switched on the concomitant expression of GFP and a miR-30 precursor, the subsequent processing of which released the embedded PTEN-targeted shRNA. The efficacy and reversibility of PTEN knockdown by this construct was validated in normal and neoplastic cells, in which PTEN deficiency resulted in accelerated cell proliferation, suppressed apoptosis, and increased invasiveness. Transgenic mice harboring the conditional shRNA-expression cassette were obtained; GFP expression and concurrent PTEN silencing were observed upon ectopic expression of rtTA and induction with Dox. Therefore, this study provides novel tools for the precise dissection of PTEN functions and the generation of PTEN loss of function models in specific subsets of cells during carcinogenesis and ontogenesis.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.117-121
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• 2004

Soluble proteins of silkworm hemolymph were investigated by means of isoelectric focusing (IEF). The protein spectra during ontogenesis of races and inter-races hybrids kept in Bulgaria was studied. A total of 51 protein bands in the hemolymph from fourth larval instar to imago were ascertained. Stage specific expression was established. The specific expression of some protein bands in the individual spectra manifest phenotype of gene determinate polymorphism (HP F, HP J, HP K, HP L, HP Q - in the zone with pH gradient 3.5-6.2 and HP K, HP L, HP N, HP P, HP T - in the zone with pH gradient 9.5 - 6.2). Breed specific expression was observed. On the basis of the obtained results, it was established that the investigated breeds are heterogeneous and the isoelectric focusing method is successful when specifying the inner-race and inter-race polymorphism in silkworm.

• 한국약용작물학회지
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• 제10권4호
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• pp.298-302
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• 2002

The essential oils were isolated by hydrodistillation and their composition determined capillary GC method with standards. The essential oil content showed significant differences between the two populations on the vegetative organs. The essential oil level of the leaves and roots was considerably higher in the Korean population at full flowering and waxy ripening stage but essential oil content of the roots was significantly higher in the Hungarian taxon at leaf rosette stage. We observed the essential oil accumulation tendency was mianly dependent on plant organs and intra-specific taxon during the vegeation period. Butylidene-phthalide was proved to be the main component of the oil in both population roots (50.9-73.3%), while dimethyl-acetate was showed as a major compound on the over-ground parts (56.7-62.0%). The qualitative composition of the essential oil in the reproductive organs concerning the identified compounds was the same as the vegetative parts with the main component ${\alpha}-phellandrene$ (4.8-28.1%) and butylidene-phtalide (9.7-16.1%), The quantitative composition showed some changes during the ontogenesis phases. Most characteristic ones are the decreasing proportion of dimethyl-acetate (from 7.3% to 1.1%) and the appearance of ${\alpha}-pinene$ (from 0.5% to 1.5%) only after fruit setting in both population.

• 한국한의학연구원논문집
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• 제3권1호
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• pp.229-239
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• 1997

The superoxide anion radical$(O_2)$ poses a threat to macromocules and cell organelles of the living cells. This toxicity damage to all groups of proteins results in loss of enzyme function concerned with metabolism and ion transport, and peroxidation of unsaturated fatty acids and cholesterol results in a change of permeability characteristics of the membrane, and oxidative of nucleic acids results in genomic damage and thereby cause mutation, potential carcinogenesis and somatic damage that produce cellular aging Superoxide dismutase(SOD) has received substantial attention as a potential therapeutic agent. It has been investigated as a possible agent for the prevention of ontogenesis, the reduction of cytotoxic effect of anticancer drugs, and protection against damage in ischemic tissue. It is suggest that $O_2$ is concerned with cellular aging, thereafter we need to investigate herb that activated to SOD.

• 수산해양기술연구
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• 제51권2호
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• pp.265-271
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• 2015

The feeding habits of Okamejei kenojei were studied using 242 specimens collected from October 2010 to September 2011 in the coastal waters of Gadeok-do, Korea. O. kenojei was carnivorous feeder that consumed mainly shrimps, crabs, and fishes. Its diet also included small quantities of stomatods, cephalopods, amphipods. The size of O. kenojei ranged from 10.4 to 47.4 cm in total length (TL). O. kenojei showed ontogenetic dietary shift. <15.0, 15.0-19.9 cm size classes fed mainly on shrimps and amphipods. 20.0-24.9 cm size class fed mainly shrimps and crabs. Thereafter, shrimps and crabs were decreased with ontogenesis while fishes and cephalopods were increased. Increasing size, the mN/ST were constantly decreased, but mW/ST were constantly increased.

• 한국어류학회지
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• 제21권4호
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• pp.227-238
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• 2009

Oryzias dancena의 난발생 과정과 자치어의 형태발달에 관하여 관찰하였다. 수온 $25{\pm}1^{\circ}C$에서 수정란은 수정 후 1시간이 경과하면 배반이 형성되었다가 30분의 간격으로 4세포기까지 난할이 이루어졌다. 이후로 발생의 속도가 느려져 수정 후, 7시간 45분이 경과된 후 포배기에 이르렀다. 수정 후 5시간 30분 후에 상실기에 도달하였으며, 포배기는 수정 후 7시간 45분 후에 이루어졌다. 수정 후 11시간 30분이 경과된 후 낭배기에 이르렀고, 수정 후 1일 6시간 후, 신경배를 형성하였다. 심장 박동이 시작되는 시기는 수정 후 2일 4시간째에 이루어졌으며, 수정 후 6일 12시간째 장기에 혈관이 형성되었다. 수정 후 11일째에 부화하였고, 부화 직후 자어는 전장이 $4.40{\pm}0.24mm$였으며, 부화 3일 후에 난황이 완전히 흡수되어 후기 자어기로 이행하였다. 부화 후 21일째에 각 지느러미 기조가 완전하게 형성되어 치어기로 이행하였으며, 이 때 전장은 $8.67{\pm}0.87mm$였다. 최초 산란이 이루어지는 시기는 부화 후 9주째로 전장은 $22.58{\pm}2.73mm$였다.