• Title/Summary/Keyword: One-pot

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Interspecific Hybridization between Populus caspica L. × P. deltoids L 62/154 Using in vitro Embryo Development and Germination

  • Ali, Jafari mofidabadi;Mansooreh, Kamandloo;Hamid, Selamti
    • Journal of Forest and Environmental Science
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    • v.33 no.3
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    • pp.197-201
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    • 2017
  • Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

Suppression Effect of Clubroot Disease by Reduced Irrigation Times after Planting in Chinese Cabbage (정식 후 초기 관수횟수 감소에 따른 배추 뿌리혹병 발생 억제효과)

  • Chang, Seog-Won;Kim, Hee-Dong;Kim, Sung-Kee;Yi, Eun-Seob;Rho, Yong-Taek
    • Research in Plant Disease
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    • v.14 no.2
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    • pp.85-89
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    • 2008
  • Clubroot of Chinese cabbage, caused by Plasmodiophora brassicae, is one of the most serious plant diseases in the world. The objectives of this study were to investigate the effect of the irrigation times on the development of club root after planting and to evaluate the effect of clubroot disease on yield of chinese cabbage in pot and field. The clubroot disease gradually increased with increasing irrigation frequency after planting. The growth characters and yield of chinese cabbage by irrigation times varied according to soil moisture and clubroot disease severity. Based on marketable yield and control value, an ideal irrigation point was at the soil matric potential of 0.06 MPa. These results suggest that the suppression on clubroot disease development could be achieved by initially reduced irrigation times after planting. Future studies on irrigation times using fungicide treatment and resistant cultivar under natural field conditions are required to improve the control effect of clubroot.

High-density genetic mapping using GBS in Chrysanthemum

  • Chung, Yong Suk;Cho, Jin Woong;Kim, Changsoo
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.57-57
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    • 2017
  • Chrysanthemum is one of the most important floral crop in Korea produced about 7 billion dollars (1 billion for pot and 6 billion for cutting) in 2013. However, it is difficult to breed and to do genetic study because 1) it is highly self-incompatible, 2) it is outcrossing crop having heterozygotes, and 3) commercial cultvars are hexaploid (2n = 6x = 54). Although low-density genetic map and QTL study were reported, it is not enough to apply for the marker assisted selection and other genetic studies. Therefore, we are trying to make high-density genetic mapping using GBS with about 100 $F_1s$ of C. boreale that is oHohhfd diploid (2n = 2x = 18, about 2.8Gb) instead of commercial culitvars. Since Chrysanthemum is outcrossing, two-way pseudo-testcross model would be used to construct genetic map. Also, genotype-by-sequencing (GBS) would be utilized to generate sufficient number of markers and to maximize genomic representation in a cost effective manner. Those completed sequences would be analyzed with TASSEL-GBS pipeline. In order to reduce sequence error, only first 64 sequences, which have almost zero percent error, would be incorporated in the pipeline for the analysis. In addition, to reduce errors that is common in heterozygotes crops caused by low coverage, two rare cutters (NsiI and MseI) were used to increase sequence depth. Maskov algorithm would also used to deal with missing data. Further, sparsely placed markers on the physical map would be used as anchors to overcome problems caused by low coverage. For this purpose, were generated from transcriptome of Chrysanthemum using MISA program. Among those, 10 simple sequence repeat (SSR) markers, which are evenly distributed along each chromosome and polymorphic between two parents, would be selected.

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A study on early industrial design in America from 1920s to 1930s (미국 산업디자인 정착기 환경에 관한 연구)

  • 신명철
    • Archives of design research
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    • v.15 no.2
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    • pp.243-252
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    • 2002
  • Industrial design has been need to made a new tool for human with adaptable to situation the age. But, at early America, it is very difficult that role of design settle down and progress than these days. It is not to he easy newly born in industrial design because of rapidly changing the America situation with multi settlers, the Depression and world wars. There was various design change in short period. So we called the step modern 1920 and the streamlined 1930. The study is background of American design by variety cultures & creatures from 1920s to 1930s. Some of Korea design has affected of America cultures at duration of 6.25 war and now. This study has included early culture & industrial life, designer activity, and at the part of rear new invention, industrial design relate to architect, four leading designers Walter Dorwin league, Norman Bel Geddes, Henry Dreyfuss, Raymond Loewy. In present world situation, It is important in all aspect to understand that early process at America industrial design. Early American culture is constructed from the building block of the puritan ethic and is sheathed by liberal mercantilism, today is became one stir it into the common molting pot.

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A Survey of Satisfaction and Preference for Military Meal Service and Food Behaviors and Food Habits of Some Military Personnel (일부 군인들의 식행동 및 식습관과 군대급식에 대한 만족도와 기호도 조사)

  • Kim Eun-Sil;Jung Bok-Mi
    • Korean Journal of Community Nutrition
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    • v.11 no.4
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    • pp.520-533
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    • 2006
  • The purpose of this study was to investigate satisfaction and preference for current military meal service and food behaviors and food habits of some military personnel in Chunchen and Wonju of the Kwangwon area. The results are as follows. In the anthropometric survey, average height of the subjects was 175.03 $\pm$ 5.30 cm, average weight was 69.15 $\pm$ 7.74 kg. As a result of surveying satisfaction in the current food service, an item high in satisfaction was adequacy of serving hours, whereas an item of low satisfaction was diversity of menu. The item of food service improvement needs of the subjects were in order more variety of menu, nutrition, preference, sanitation, cooking method. As a result of surveying eating behavior of the subjects, most items were high medium response. They preferred soju and relish and also they liked the bokkum cooking method. As a result of surveying food habits of the subjects, items of good habits were regularity of meals, marginal meals, diversity of food intake, dairy and dairy products, avoiding too exciting meals and natural food diet; whereas, items of bad habits were fast food intake, alcohol, smoking, carbonated and caffeine beverages, skipping breakfast. snacks, fatty food intake, yellow or green vegetable intake and fruit and seaweed intake. Following is the result of a survey on the preference of meals by cooking method serving the current military meal service. They liked one-dish meals such as Bokkeumbap, Bibimbap, Tteokguk, whereas, they disliked curryrice, Jajangbap. In case of soup, they liked soup made from meat better than vegetable soup or soup made with fish. Also they liked pot stem better than soup. Generally they liked meals containing meat in most cooking methods. These results suggest that continuous and practical nutrition education to change food habits in military life are necessary to Prevent chronic disease with increasing age.

Bimetallic Co/Zn-ZIF as an Efficient Photocatalyst for Degradation of Indigo Carmine

  • Nguyen, Thanh Nhan;Nguyen, Hoang Phuc;Kim, Tae-Ho;Lee, Soo Wohn
    • Korean Journal of Materials Research
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    • v.28 no.1
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    • pp.68-74
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    • 2018
  • Cobalt-incorporated zeolitic imidazolate framework ZIF-8 was synthesized by a simple one-pot synthesis method at room temperature. Powder X-ray diffraction patterns and energy dispersive X-ray spectrum confirmed the formation of the bimetallic Co/Zn-ZIF structure. UV-Vis diffuse reflectance spectra revealed that the bimetallic ZIF had a lower HOMO-LUMO gap compared with ZIF-8 due to the charge transfer process from organic ligands to cobalt centers. A hydrolytic stability test showed that Co/Zn-ZIF is very robust in aqueous solution - the most important criterion for any material to be applied in photodegradation. The photocatalytic efficiency of the synthesized samples was investigated over the Indigo Carmine (IC) dye degradation under solar simulated irradiation. Cobalt incorporated ZIF-8 exhibited high efficiency over a wide range of pH and initial concentration. The degradation followed through three distinct stages: a slow initial stage, followed by an accelerated stage and completed with a decelerated stage. Moreover, the photocatalytic performance of the synthesized samples was highly improved in alkaline environment rather than in acidic or neutral environments, which may have been because in high pH medium, the increased concentration of hydroxyl ion facilitated the formation of hydroxyl radicals, a reactive species responsible for the breaking of the Indigo Carmine structure. Thus, Co/Zn-ZIF is a promising and green material for solving the environmental pollution caused by textile industries.

Fishing Mechanism of Pots and their Modification 2. Behavior of Crab, Charybdis japonica, to Net Pots (통발어구의 어획기구 및 개량에 관한 연구 2. 그물통발류에 대한 민꽃게의 행동)

  • KIM Dae-An;KO Kwan-Soh
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.4
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    • pp.348-354
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    • 1987
  • The behavior of crab, Charybdis japonica h. Milne EDWARDS, to the net pots with baits was investigated alternately in the experimental tanks. One of the pots being dropped on the tank bottom, the crabs touched it to obtain the bait probably reacted by their senses of smell and sight, and increased gradually in the number of touch to show a maximum within 30 min. The crabs, if touched circular pots, were guided more easily to the pot entrances than the case of touching square ones, but the guidance from the vicinity of the entrances into the pots was easier in the square. When the crabs entered the pots, they always showed a sharp precaution. However, most of enterings were made mainly within 30 minutes and easier in pots with lower entrances. If 30 min. elapsed, the entering was little made by the decrease in the number of touch and the getting-out was remarkable, especially in pots with low entrances. But, in all the pots the getting-out was hampered by the drawing of the entrance tips into the pots. In case in which flappers were attached to the entrance tips, the entering was very hampered, hut the getting-out was not shown.

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The Hot Spot in Superior Vena Caval Obstruction Using $^{99m}Technetium$ tin Colloid ($^{99m}Tc-tin$ colloid를 이용(利用)한 간(肝)스캔상(上) "Hot spot"로 나타난 상대정맥증후군(上大靜脈症候群) 1예(例))

  • Kim, Byung-Tae;Kwon, Kye-Ik;Shin, Young-Tae;Cho, Kyung-Sam;Lee, Myung-Chul;Cho, Bo-Yeon;Koh, Chang-Soon
    • The Korean Journal of Nuclear Medicine
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    • v.15 no.1
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    • pp.45-49
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    • 1981
  • The hot spot on liver scan was demonstrated by many authors in various conditions such as SVC obstruction, Budd-Chiari syndrome, liver abscess, hemangioma of liver, hepatic venoocclusive diseases, IVC obstruction, and tricuspid insufficiency. And the appearance of hot spot in SVC obstruction is due to unsual collateral circulation. But there was no report of this hots pot on liver scan in our country. We have recently observed one patient with SVC obstruction who shows well-defined area of increased radioactivity between right and left lobe of liver on liver scan using $^{99m}Tc-tin$ colloid, and demonstrated collateral circulations with RI venography using $^{99m}Tc-O_4$. The injection site of radiocolloid was left antecubital vein. This hot spot did not appear when the radiocolloid was injected into right leg vein. We report here this hot spot on liver scan in SVC obstruction with review of some liter atures.

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Effect or $\gamma$-Radiation on Growth and Antioxidant Enzyme Activities in Red Pepper (방사선처리가 고추의 생육과 항산화 효소의 활성에 미치는 영향)

  • 권순태;정은아;김재성
    • Journal of Life Science
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    • v.11 no.6
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    • pp.612-617
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    • 2001
  • Seedlings of red pepper (Capsicum annum L.) were exposed to various doses of ${\gamma}$-radiation )($^{60}$ Co)and cultivated in the 1/2,000 a pot. Plant hight the number of leaves, flowers and fruits, chlorophyll contents, plant dry weight, ac- tivites of antiozidant enzymes such as superoxide dismutase(SOD) adn peroxidase(POD), were determined. Plant height of seedling treated with 1 Gy of ${\gamma}$-radiation was increased up to 16.6% as compared to untreated control at 80 days after treatment, while those treated with 20 Gy or 50 Gy was decreased 22.0% or 75.0% respectively. The plants treated with 1 Gy or 5 Gy were increased the number of leaves up to 41.2% and 24.9% as well as dry weight of fruit up to 58.4% and 49.4% respectively. However treatment of radiation higher than 20 Gy significantly inhibited the growth, chlorophyll content and yield of pepper fruit. Activities of antioxidant enzymes, SOD and POD were tem-porary increased within one or five days after treatment depending on the doses of treatment , and the activities were gradually decreased to control level of lower thereafter. The SOD and POD activities in the leaves of pepper plant treated with 50 Gy were 5.5 and 6.0folds higher than that of control at five days after treatment, respectively.

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Effect of 3-Isocaynatopropyl Triethoxy Silane on PU Adhesive (3-Isocaynatopropyl Triethoxy Silane이 PU 접착제에 미치는 영향)

  • Ju, Honghee;Jang, Euisub;Park, Chan Young;Lee, Won-Ki;Kim, Taekyun;Chun, Jae-Hwan
    • Journal of Adhesion and Interface
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    • v.14 no.1
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    • pp.36-42
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    • 2013
  • To improve hydrolytic stability of polyurethane (PU) adhesives, a silane coupling agent (SCA) was added. 3-Isocyanatopropyl triethoxy silane (ITS) as a SCA has two functional groups in the main chain and it is used to improve an interfacial interaction between polymer and inorganic material or metal. In this study, PU adhesives with different amounts of ITS from 0 to 1 wt% were synthesized. Pot time, modulus, thermal stablilty, and adhesive force of the obtained samples were measured. The results showed that the adhesives with ITS showed better properties than that of pure one.