• Title/Summary/Keyword: One-pot

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Effect of the different cover crop incorporation on glomalin-related soil protein and soybean and maize growth

  • Higo, Masao;Gunji, Kento;Isobe, Katsunori
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.344-344
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    • 2017
  • The glycoprotein known as glomalin-related soil protein (GRSP) is abundantly produced on the hyphae and spores of arbuscular mycorrhizal fungi (AMF) in soil and roots. GRSP play a decisive role in the soil aggregation, but GRSP was also sensitive to agricultural managements. Thus, our objectives were to assess the effect of different cover crop incorporation on the GRSP content in soil and growth of subsequent soybean and maize. Pot experiments with the incorporation of four cover crops were set up. The same amount (666g) of aboveground plant parts of wheat (AMF host), hairy vetch (AMF host), mustard (non-host) and rapeseed (non-host) was separately incorporated into soils. The aboveground plant parts and roots of soybean and maize were grown in each incorporated pots and sampled at 6 and 9 weeks after sowing. Our results showed that the different cover crops incorporation affected soil biological and chemical properties such as EC, $NO_3-N$ content, ${\beta}-glucosidase$ activity, alkaline phosphatase (ALP) activity and GRSP content. The soil EC and $NO_3-N$ content in the hairy vetch, mustard and rapeseed was higher compared to the wheat. The ${\beta}-glucosidase$ activity in the wheat and hairy vetch was significantly higher than that in the mustard and rapeseed, and the ALP activity in the wheat was significantly higher than that in the hairy vetch, mustard, and rapeseed. The GRSP content in the mustard and rapeseed was significantly lower than that of the hairy vetch and wheat. Moreover, The top dry weight and leaf area of soybean and maize in the hairy vetch at 6 weeks were significantly higher compared to the other treatments. Our results indicated that the incorporation of mustard and rapeseed may cause indirectly the decrease of GRSP content and soil enzyme activity in soil. One possible explanation for the decrease of GRSP in non-AMF host crop treatments may be the decrease of AMF density in the soil. AMF are not able to form a symbiotic relationship with Brassicaceae roots due to the release of anti-fungal compounds. This means the AMF may not be able to produce GRSP in the soil. However, the differences in the benefit of cover crop incorporation were shown only by a pot experiment. Comparative investigations of crop residue managements would be applied to both pot experiment and field study to clarify a better selection of cover crops in rotation to encourage GRSP production.

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Effects of root nodules on the plant type in soybean-Especially internode length and petiole length on the main stem

  • Ohashi, Shuma;Kurita, Haruna;Takahashi, Yukitsugu;Nagasuga, Kiyoshi;Nagaya, Yuichi;Umezaki, Teruhisa
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.358-358
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    • 2017
  • The plant type is generally one of the most important factor for crop production and be influenced by nitrogen absorption. Soybean plants have nodules in their roots, supplying nitrogen at the vegetative and reproductive stages. Root nodules seem to effect plant type of soybean plants, but there are few reports on the relation nodules and plant type. We tried to clarify the effects of root nodules on the plant type, especially internode length and petiole length, comparing non-nodule soybean with normal soybean. The pot experiment and field experiment were carried out at Mie University and Utsunomiya University in 2015 and 2016. Enrei, a popular cultivar in central Japan, and En1282, non-nodulating isogenic line of Enrei, were used. The petiole length on main stem was measured after defoliation and internode length and yield components were measured after harvest. In the field experiment, the patterns of the final length in internode and petiole on main stem were consistent in both cultivars, and a positive correlation was found between the Nth petiole length and the N-1th internode length, belong to the same phytomere. Therefore, the petiole and internode on the main stem make similar response for environmental factors. In pot experiment, Enrei grew with the same pattern as field experiments, but in En1282, the elongation of petiole and internode in the upper part was suppressed, especially the petiole was suppressed greatly. The main stem becomes the basis of the plant type. These results were considered that the nitrogen is distributed preferentially to the internode than the petiole. It seems that the pot cultivation restricted the rhizosphere and caused nitrogen deficiency in En1282. These results suggested that the slight nitrogen deficiency provided from the root nodules was compensated by the increase of the amount of inorganic nitrogen absorption due to the expansion of the rhizosphere, and the severe nitrogen deficiency suppressed elongation of petiole and internode. It is clear that root nodules effect the plant type by supplying nitrogen to internodes and petioles.

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Effect of Alfalfa Extract, It's Concentration and Absorbents on Germination and Growth of Alfalfa (알팔파 추출물, 추출농도 및 흡착제 처리가 알팔파 발아와 생육에 미치는 영향)

  • Ill Min, Chung;Ki-June Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.5
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    • pp.619-628
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    • 1995
  • Crude aqueous extracts from dried leaves, stems, roots, and flowers from both field grown and greenhouse grown alfalfa plants inhibited alfalfa seed germination and seedling growth. The degree of inhibition was greater in the field grown plant extracts. Flowers extract of field grown plant most inhibited alfalfa germination and seedling growth. In the concentration study, the highest concentration of extract (9.0%, w/v) significantly inhibited total alfalfa seed germination by 50% as compared to control. In partitioning study using pot hydroponic culture of plant biomass into leaves, stems, root, LAR products of LWR and SLA exhibited significant variation among four species. This result support that the inhibitory effect of autotoxic substances presenting in alfalfa tissue may be possible interference with the patitioning of biomass into leaf component relative to the total biomass produced by the alfalfa plant. Toxicity of extract was not reduced by adding activated charcoal, Dowex-50W, amberlite to the extract. Toxic substances existing in most plant tissues but mainly above ground foliage are water soluble and stable and may persist in old alfalfa fields. Thus, it is recommended to remove as much as possible of the above growth parts, especially vegetative stage, before one tries to re-establish alfalfa in former field of alfalfa.

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Fishing Mechanism of Pots and their Modification 3. Behavior of Conger Eel, Astroconger myriaster, to Net Pots (통발어구의 어획기구 및 개량에 관한 연구 3. 그물통발유에 대한 붕장어의 행동)

  • KIM Dae-An;KO Kwan-Soh
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.3
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    • pp.238-244
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    • 1990
  • The behavior of conger eel, Astroconger myriaste(Brevoort), to the net pots with baits was investigated alternately in two experimental water tanks. One of the pots being dropped on the tank bottom, the eels increased rapidly in number of touching the pot to show a maximum whithin 30 minutes and then decreased gradually. The enterings of eels into the pots were also made mainly within 30 minutes and easier in pots with lower entrances. But the complete enterings were decided by the passage of eel tails through the pot entrances and so the eels retreated frequently after attemping to enter the pots, especially in pots small in the interval between the entrances and the baits or in pots with flappers. However, most of enterings encouraged the touches and earlier enterings ascribed earlier production of maximum touch. The eels, if entered the pots without flappers, got out of the pots relatively easily and the getting-out was easier in pots with lower entrances. On the other hand, the eels entered the pot with flappers could not get out of the pots. A comparison between the circular and square pots gave that the guidance of eels to the entrances was easier in the former and the passing of eels through the entrances in the latter, not only at the inside of pots but also at the outside. The general behavior of eels in touching or entering the net pots in the above results was almost equal to that on the bamboo and plastic pots in the previous paper.

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Optimum Media of Sedum kamtschaticum for Pot Culture in an Ebb & Flow System (Ebb & Flow 시스템에서 기린초 분화재배에 적합한 배지 선발)

  • Kim, Su Jeong;Chang, Dong Chil;Suh, Jong Taek;Lee, Eung Ho
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.3
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    • pp.205-210
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    • 2008
  • Sedum kamtschaticum has been used as one of the native valuable plants for ornamental purpose. This experiment was conducted to find out the optimum media for pot cultured Sedum kamtschaticum using peatmoss and coir-based mixtures. Four mixtures such as peatmoss:perlite(5:5, v/v), peatmoss:perlite(7:3, v/v), coir:perlite(5:5, v/v), and coir:perlite(7:3, v/v) were used. Plants were irrigated with a Sonneveld solution in an ebb & flow system. The content of T-N, K, and Ca in the peatmoss-based media was higher than those of the coir-based media. The concentration of T-N, K, and Ca in the shoots of Sedum kamtschaticum increased when plants were cultured with peatmoss-based media. In the peatmoss-based media, plant growth, such as number of branches, leaf length and shoot fresh and dry weights, were significantly increased than those of plant grown in the coir-based media. As the results, the optimum media for pot cultured Sedum kamtschaticum in an ebb & flow system was considered to be the peatmoss-based mixtures.

Growth of One-Year-Old Pot-Cultivated 'Fuji'/M.9 Apple Trees under Different Concentrations of Nitrogen Fertilization (질소시비농도에 따른 1년생 사과 'Fuji'/M.9 포트묘목의 수체 생장)

  • Ha, Woongyong;Shin, Hyunsuk;Lim, Heon-Kyu;Oh, Youngjae;Han, Hyeondae;Kim, Keumsun;Oh, Sewon;Kwon, Yeuseok;Kim, Daeil
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.499-508
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    • 2019
  • The study was carried out to investigate growth of 48.6-L pot-cultivated 1-year-old 'Fuji'/M.9 apple trees depending on different levels of nitrogen concentration. While rise in tree growth was paralleled with increase of nitrogen concentration, more than 32 mM of nitrogen rather restrained tree growth. In particular, growth of 16 mM of nitrogen treated trees was satisfied with criteria for production of high-quality pot-cultivated nursery stocks. Although mineral contents of leaves were higher in 8 and 16 mM nitrogen treatments than commonly recommended mineral contents in apple orchards, such somewhat surplus minerals could be helpful for tree growth after transplanting to apple orchards. In addition, our result indicated that soils of 8 and 16 mM of nitrogen treated pots met appropriate criteria for soil chemical property of apple orchards. Thus, in the light of tree growth, mineral contents of leaves, and soil chemical property in the pots, 16 mM of nitrogen treatment is considered to be suitable for production of 1-year-old 'Fuji'/M.9 apple potted trees.

Effects of Seed Size and Weight on Growth of First-Year Seedling in Pinus koraiensis (잣나무종자(種子)의 크기와 무게가 묘목(苗木)의 초기생장(初期生長)에 미치는 영향(影響))

  • Chon, Sang Keun
    • Journal of Korean Society of Forest Science
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    • v.31 no.1
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    • pp.48-52
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    • 1976
  • In order to investigate the effects of seed weight, size (length and thickness), and maturation period (early maturing seed or late maturing seed) on growth of first-year seedlings in Pinus koraiensis, pot cultivation experiment with seeds collected from Gangweon University Forest was carried out under relatively controlled environment condition. At the end of one growing season, not only fresh weight, length, diameter (at underside of cotyledon) and root length of seedlings, but also number and length of cotyledon were measured. Results are as follows; 1. Germination percentage is independent of weight and size of seeds, but it was influenced by maturation period, that is, late maturing seeds surpass early maturing one in germination percentage. 2. Germination percentage is affected by maturation period of seeds in 18.3% of contribution rate. 3. Each growth of first-year seedlings is influenced considerably by the differences of weight, length and thickness of seeds, that is, heavy and large seeds produce larger seedlings than do light and small seeds. Particularly, fresh weight, diameter, length of cotyledon are significantly affected by differences of seed weight and size (length and thickness) in 50-90% of contribution rate. 4. Differences of seed weight and thickness have a tendency to affect more on growth of first-year seedlings than do differences of seed length. 5. Seedlings from late maturing seeds are superior than those from early maturing one in fresh weight, diameter of seedlings and length of cotyledon, but it's influence is a little.

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Methane Conversion to Hydrogen Using Ni/Al2O3 Catalyst (Ni/Al2O3 촉매를 이용한 메탄의 수소 전환)

  • Kim, Jun-Keun;Park, Joo-Won;Bae, Jong-Soo;Kim, Jae-Ho;Lee, Jae-Goo;Kim, Younghun;Han, Choon
    • Applied Chemistry for Engineering
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    • v.19 no.5
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    • pp.466-470
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    • 2008
  • The objective of this study is to convert methane into hydrogen using a nanoporous catalyst in the $CO_2$ containing syngas generated from the gasified waste. For the purpose, $Ni/Al_2O_3$ catalyst was prepared with the one-pot method. According to analyses of the catalyst, three dimensionally linked sponge shaped particles were created and the prepared nanoporous catalysts had larger surface area and smaller particle size and more uniform pores compared to the sphere shaped commercial catalyst. The catalyst for reforming reaction gave the highest $CH_4$ conversion of 91%, and $CO_2$ conversion of 92% when impregnated with 16 wt% of Ni at the reaction temperature of $750^{\circ}C$. At that time, the prepared catalyst remarkably improved the $CH_4$ and $CO_2$ conversion up to 20% compared to the commercial one.

Grain Yield and Physiological Responses of Water Stress at Reproductive Stage in Barley (보리 생식생장기의 수분부족이 수량 및 몇 가지 생리적 반응에 미치는 영향)

  • Choi, Won-Yul;Kwon, Yong-Woong;Park, Jong-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.3
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    • pp.263-269
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    • 1997
  • To cope with increasing importance of water stress in food crop production, some physiological characteristics, their cultivar-differences and grain yield of winter barley cultivars in response to water stress during reproductive stages were studied employing three covered-barley cultivars, Milyang 12, Durubori, and Olbori, one naked-barley cultivar, Baegdong, and one two-row malting-barley cultivar, Hyangmaeg. The barley grown in pot-soil was conditioned for 10 days under water stress, varying the time of water stress : 20 days before heading, 10 days before heading and the time of heading. The decrease in growth due to water stress varied greatly with the cultivars and time of water stress. The greatest injury occurred when water stress was imposed for 10 days from 10 days before heading : the culm length of water-stressed plants have shown reduced by 85∼98% of the non-stressed; the number of spikes per plant by 52∼83%; the number of grains per spike by 71∼86%; 1,000-grain weight by 80∼84%; yield per pot by 60∼94%. The number of spikes per plant as one of yield components was most sensitively affected. As a whole, the drought resistance of cultivars was high in the order of Olbori> Milyang 12 and Durubori> Hyangmaeg>Baegdong. On rewatering the plants after termination of the water stress treatment the recovery rate of free proline content and relative turgidity of flag leaf were higher in 3 covered-barley cultivars, and lower in cultivars Baegdong and Hyangmaeg.

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Discovery and Molecular Engineering of Sugar-containing Natural Product Biosynthetic Pathways in Actinomycetes

  • Oh, Tae-Jin;Mo, Sang-Joon;Yoon, Yeo-Joon;Sohng, Jae-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.17 no.12
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    • pp.1909-1921
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    • 2007
  • Significant progress has recently been made concerning the engineering of deoxysugar biosynthesis. The biosynthetic gene clusters of several deoxysugars from various polyketides and aminoglycosides-producing microorganisms have been cloned and studied. This review introduces the biosynthetic pathways of several deoxysugars and the generation of novel hybrid macrolide antibiotics via the coexpression of deoxysugar biosynthetic gene cassettes and the substrate-flexible glycosyltransferases in a host organism as well as the production of TDP-deoxysugar derivatives via one-pot enzymatic reactions with the identified enzymes. These recent developments in the engineering of deoxysugars biosynthesis may pave the way to create novel secondary metabolites with potential biological activities.