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Discovery and Molecular Engineering of Sugar-containing Natural Product Biosynthetic Pathways in Actinomycetes  

Oh, Tae-Jin (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, SunMoon University)
Mo, Sang-Joon (Division of Nano Sciences and Department of Chemistry, Ewha University)
Yoon, Yeo-Joon (Division of Nano Sciences and Department of Chemistry, Ewha University)
Sohng, Jae-Kyung (Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, SunMoon University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.12, 2007 , pp. 1909-1921 More about this Journal
Abstract
Significant progress has recently been made concerning the engineering of deoxysugar biosynthesis. The biosynthetic gene clusters of several deoxysugars from various polyketides and aminoglycosides-producing microorganisms have been cloned and studied. This review introduces the biosynthetic pathways of several deoxysugars and the generation of novel hybrid macrolide antibiotics via the coexpression of deoxysugar biosynthetic gene cassettes and the substrate-flexible glycosyltransferases in a host organism as well as the production of TDP-deoxysugar derivatives via one-pot enzymatic reactions with the identified enzymes. These recent developments in the engineering of deoxysugars biosynthesis may pave the way to create novel secondary metabolites with potential biological activities.
Keywords
Actinomycetes; combinatorial biosynthesis; deoxysugar; glycosyltransferase; natural product;
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Times Cited By KSCI : 15  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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1 Aguirrezabalaga, I., C. Olano, N. Allende, L. Rodriguez, A. F. Brana, C. Mendez, and J. A. Salas. 2000. Identification and expression of genes involved in biosynthesis of Loleandrose and its intermediate L-olivose in the oleandomycin producer Streptomyces antibioticus. Antimicrob. Agents Chemother. 44: 1266-1275   DOI   ScienceOn
2 Bechthold, A., J. K. Sohng, T. M. Smith, X. Chu, and H. G. Floss. 1995. Identification of Streptomyces vialaceoruber Tu22 genes involved in the biosynthesis of granaticin. Mol. Gen. Genet. 248: 610-620   DOI   ScienceOn
3 Chong, Y. H., J. M. Young, J. Y. Kim, Y. Y. Lee, K. S. Park, J. H. Cho, H. J. Kwon, J. W. Suh, and Y. H. Lim. 2006. S-Adenosyl-L-methionine analogues to enhance the production of actinorhodin. J. Microbiol. Biotechnol. 16: 1154-1157   과학기술학회마을
4 Chung, Y. S., D. H. Kim, W. M. Seo, H. C. Lee, K. Liou, T. J. Oh, and J. K. Sohng. 2007. Enzymatic synthesis of dTDP-4-amino-4,6-dideoxy-D-glucose using GerB (dTDP- 4-keto-6-deoxy-D-glucose aminotransferase). Carbohydr. Res. 342: 1412-1418   DOI   ScienceOn
5 Demain, A. L. 1999. Pharmaceutically active secondary metabolites of microorganisms. Appl. Microbiol. Biotechnol. 52: 455-463   DOI   ScienceOn
6 Fu, X., C. Albermann, C. Zhang, and J. S. Thorson. 2005. Diversifying vancomycin via chemoenzymatic strategies. Org. Lett. 7: 1513-1515   DOI   ScienceOn
7 Huang, F., S. F. Haydock, T. Mironenko, D. Spiteller, Y. Li, and J. B. Spencer. 2005. The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: Characterization of an aminotransferase involved in the formation of 2-deoxystreptamine. Org. Biomol. Chem. 3: 1410-1419   DOI   ScienceOn
8 Jung, W. S., A. R. Han, J. S. Hong, S. R. Park, C. Y. Choi, J. W. Park, and Y. J. Yoon. 2007. Bioconversion of 12-, 14-, and 16-membered ring aglycones to glycosylated macrolides in an engineered strain of Streptomyces venezuelae. Appl. Microbiol. Biotechnol. 76: 1373-1381   DOI   ScienceOn
9 Kang, Y. B., Y. H. Yang, K. W. Lee, S. G. Lee, J. K. Sohng, H. C. Lee, K. Liou, and B. G. Kim. 2006. Preparative synthesis of dTDP-L-rhamnose through combined enzymatic pathways. Biotechnol. Bioeng. 93: 21-27   DOI   ScienceOn
10 Lee, S. K., J. W. Park, J. W. Kim, W. S. Jung, S. R. Park, C. Y. Choi, E. S. Kim, B. S. Kim, J. S. Ahn, D. H. Sherman, and Y. J. Yoon. 2006. Neopikromycin and novapikromycin from the pikromycin biosynthetic pathway of Streptomyces venezuelae. J. Nat. Prod. 69: 847-849   DOI   ScienceOn
11 Lu, W., C. Leimkuhler, G. J. Jr Gatto, R. G. Kruger, M. Oberthur, D. Kahne, and C. T. Walsh. 2005. AknT is an activating protein for the glycosyltransferase AknS in Laminodeoxysugar transfer to the aglycone of aclacinomycin A. Chem. Biol. 12: 527-534   DOI   ScienceOn
12 Mason, D. J., A. Dietz, and R. M. Smith. 1961. Actinospectacin, a new antibiotic. I. Discovery and biological properties. Antibiot. Chemother. 11: 118-122
13 Oh, J., S. G. Lee, B. G. Kim, J. K. Sohng, K. Liou, and H. C. Lee. 2003. One-pot enzymatic production of dTDP-4-keto- 6-deoxy-D-glucose from dTMP and glucose-1-phosphate. Biotechnol. Bioeng. 84: 452-458   DOI   ScienceOn
14 Rodriguez, L., I. Aguirrezabalaga, N. Allende, A. F. Brana, C. Mendez, and J. A. Salas. 2002. Engineering deoxysugar biosynthetic pathways from antibiotic-producing microorganisms. A tool to produce novel glycosylated bioactive compounds. Chem. Biol. 9: 721-729   DOI   ScienceOn
15 Thuy, M. L., M. K. Kharel, R. Lamichhane, H. C. Lee, J. W. Suh, K. Liou, and J. K. Sohng. 2005. Expression of 2-deoxyscyllo- inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans. Biotechnol. Lett. 27: 465-470   DOI   ScienceOn
16 Sohng, J. K., T. J. Oh, and C. G. Kim. 1998. Method for cloning biosynthetic genes of secondary metabolites including deoxysugar from Actinomycetes. J. Biochem. Mol. Biol. 31: 475-483
17 Steffensky, M., A. Muhlenweg, Z. X. Wang, S. M. Li, and L. Heide. 2000. Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891. Antimicrob. Agents Chemother. 44: 1214-1222   DOI   ScienceOn
18 Subba, B., M. K. Kharel, H. C. Lee, K. Liou, B. G. Kim, and J. K. Sohng. 2005. The ribostamycin biosynthetic gene cluster in Streptomyces rebosidificus: Comparison with butirosin biosynthesis. Mol. Cells 20: 90-96
19 Zhu, L., X. Cao, W. Chen, G. Zhang, D. Sun, and P. G. Wang. 2005. Syntheses and biological activities of daunorubicin analogs with uncommon sugars. Bioorg. Med. Chem. 13: 6381-6387   DOI   ScienceOn
20 Huang, F., D. Spiteller, N. A. Koorbanally, Y. Li, N. M. Llewellyn, and J. B. Spencer. 2007. Elaboration of neosamine rings in the biosynthesis of neomycin and butirosin. Chembiochem 8: 283-288   DOI   ScienceOn
21 Kharel, M. K., D. B. Basnet, H. C. Lee, K. Liou, J. S. Woo, B. G. Kim, and J. K. Sohng. 2004. Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptomyces tenebrarius. FEMS Microbiol. Lett. 230: 185-190   DOI   ScienceOn
22 Newman, D. J., G. M. Cragg, and K. M. Snader. 2003. Natural products as sources of new drugs over the period 1981-2002. J. Nat. Prod. 66: 1022-1037   DOI   ScienceOn
23 Kharel, M. K., B. Subba, H. C. Lee, K. Liou, J. S. Woo, and J. K. Sohng. 2003. An approach for cloning biosynthetic genes of 2-deoxystreptamine-containing aminocyclitol antibiotics: Isolation of a biosynthetic gene cluster of tobramycin from Streptomyces tenebrarius. Biotechnol. Lett. 25: 2041-2047   DOI   ScienceOn
24 Kudo, F., M. Numakura, H. Tamegai, H. Yamamoto, T. Eguchi, and K. Kakinuma. 2005. Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073. J. Antibiot. 58: 373-379   DOI   ScienceOn
25 Maharjan, J., K. Liou, H. C. Lee, C. G. Kim, J. J. Lee, J. C. Yoo, and J. K. Sohng. 2003. Functional identification of rub52 gene involved in the biosynthesis of rubradirin. Biotechnol. Lett. 25: 909-915   DOI   ScienceOn
26 Sohng, J. K., T. J. Oh, J. H. Cha, J. J. Hahn, J. W. Kim, J. W. Suh, and H. C. Lee. 2001. Cloning and identification of a gene cluster in Streptomyces spectabilis - spectinomycin producer. J. Biochem. Mol. Biol. Biophys. 5: 209-218
27 Langenhan, J. M., N. R. Peters, I. A. Guzei, F. M. Hoffmann, and J. S. Thorson. 2005. Enhancing the anticancer properties of cardiac glycosides by neoglycorandomization. Proc. Natl. Acad. Sci. USA 102: 12305-12310
28 Lee, Y. Y., J. M. Young, H. J. Kwon, J. W. Suh, J. Y. Kim, Y. H. Chong, and Y. H. Lim. 2006. AdoMet derivatives induce the production of actinorhodin in Streptomyces coelicolor. J. Microbiol. Biotechnol. 16: 965-968   과학기술학회마을
29 Melancon, C. E. III, H. Takahashi, and H. W. Liu. 2004. Characterization of TylM3/TylM2 and MydC/MycB pairs required for efficient glycosyltransfer in macrolide antibiotic biosynthesis. J. Am. Chem. Soc. 126: 16726- 16727   DOI   ScienceOn
30 Hoeksema, H. and J. C. Knight. 1975. The production of dihydrospectinomycin by Streptomyces spectabilis. J. Antibiot. 28: 240-241   DOI
31 Tok, J. B., L. J. Dunn, and R. C. Des Jean. 2001. Binding of dimeric aminoglycosides to the HIV-1 rev responsive element (RRE) RNA constructs. Bioorg. Med. Chem. Lett. 11: 1127-1131   DOI   ScienceOn
32 Waldron, C., P. Matsushima, P. R. Jr. Rosteck, M. C. Broughton, J. Turner, K. Madduri, K. P. Crawford, D. J. Merlo, and R. H. Baltz. 2001. Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa. Chem. Biol. 8: 487-499   DOI
33 Hooper, D. C., J. S. Wolfson, G. L. McHugh, M. B. Winters, and M. N. Swartz. 1982. Effects of novobiocin, coumermycin A1, clorobiocin, and their analogs on Escherichia coli DNA gyrase and bacterial growth. Antimicrob. Agents Chemother. 22: 662-671   DOI   ScienceOn
34 Khosla, C. and R. J. X. Zawada. 1996. Generation of polyketide libraries via combinatorial biosynthesis. Trends Biotechnol. 14: 2465-2497
35 Wehrli, W. 1977. Ansamycins. Chemistry, biosynthesis and biological activity. Top. Curr. Chem. 72: 21-49   DOI
36 Madduri, K. and C. R. Hutchinson. 1995. Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. J. Bacteriol. 177: 1208-1215   DOI
37 Thuy, T. T., H. C. Lee, C. G. Kim, L. Heide, and J. K. Sohng. 2005. Functional characterizations of novWUS involved in novobiocin biosynthesis from Streptomyces spheroides. Arch. Biochem. Biophys. 436: 161-167   DOI   ScienceOn
38 Hong, J. S., W. S. Jung, S. K. Lee, W. S. Koh, H. S. Park, S. J. Park, Y. S. Kim, and Y. J. Yoon. 2005. The role of a second protein (DesVIII) in glycosylation for the biosynthesis of hybrid macrolide antibiotics in Streptomyces venezuelae. J. Microbiol. Biotechnol. 15: 640-645   과학기술학회마을
39 Rajkarnikar, A., H. J. Kwon, Y. W. Ryu, and J. W. Suh. 2007. Two threonine residues required for role of AfsKav in controlling morphogenesis and avermectin production in Streptomyces avermitilis. J. Microbiol. Biotechnol. 17: 1563-1567   과학기술학회마을
40 Doumith, M., R. Legrand, C. Lang, J. A. Salas, and M. C. Raynal. 1999. Interspecies complementation in Saccharopolyspora erythraea: Elucidation of the function of oleP1, oleG1, and oleG2 from the oleandomycin biosynthetic gene cluster of Streptomyces antibioticus and generation of new erythromycin derivatives. Mol. Microbiol. 34: 1039- 1048   DOI   ScienceOn
41 Han, J. M., S. M. Kim, H. J. Lee, and J. C. Yoo. 2007. Cloning and expression of glucose-1-phosphate thymidylyltransferase gene (schS6) from Streptomyces sp. SCC-2136. J. Microbiol. Biotechnol. 17: 685-690   과학기술학회마을
42 Lee, H. C., J. K. Sohng, H. J. Kim, D. H. Nam, C. N. Seong, J. M. Han, and J. C. Yoo. 2004. Cloning, expression, and biochemical characterization of dTDP-glucose 4,6-dehydratase gene (gerE) from Streptomyces sp. GERI-155. J. Microbiol. Biotechnol. 14: 576-583
43 Jo, Y. Y., S. H. Kim, Y. Y. Yang, C. M. Kang, J. K. Sohng, and J. W. Suh. 2003. Functional analysis of spectinomycin biosynthetic genes from Streptomyces spectabilis ATCC27741. J. Microbiol. Biotechnol. 13: 906-911
44 Park, N. S., H. J. Park, K. B. Han, and E. S. Kim. 2006. Heterologous expression of novel cytochrome P450 hydroxylase genes from Sebekia benihana. J. Microbiol. Biotechnol. 16: 295-298   과학기술학회마을
45 Graninger, M., B. Nidetzky, D. E. Heinrichs, C. Whitfield, and P. Messner. 1999. Characterization of dTDP-4- dehydrorhamnose 3,5-epimerase and dTDP-4-dehydrorhamnose reductase, required for dTDP-L-rhamnose biosynthesis in Salmonella enterica serovar Typhimurium LT2. J. Biol. Chem. 274: 25069-25077   DOI
46 He, X. M. and H. W. Liu. 2002. Formation of unusual sugars: Mechanistic studies and biosynthetic applications. Annu. Rev. Biochem. 71: 701-754   DOI   ScienceOn
47 Lamichhane, J., K. Liou, H. C. Lee, C. G. Kim, and J. K. Sohng. 2006. Functional characterization of ketoreductase (rubN6) and aminotransferase (rubN4) genes in the gene cluster of Streptomyces achromogenes var. rubradiris. Biotechnol. Lett. 28: 545-553   DOI   ScienceOn
48 Lee, H. C., J. K. Sohng, H. J. Kim, D. H. Nam, J. M. Han, S. S. Cho, J. H. Choi, and J. C. Yoo. 2004. Cloning and expression of the glucose-1-phosphate thymidylyltransferase gene (gerD) from Streptomyces sp. GERI-155. Mol. Cells 17: 274-280
49 Truman, A. W., F. Huang, N. M. Llewellyn, and J. B. Spencer. 2007. Characterization of the enzyme BtrD from Bacillus circulans and revision of its functional assignment in the biosynthesis of butirosin. Angew. Chem. Int. Ed. Engl. 46: 1462-1464   DOI   ScienceOn
50 Kim, B. C., J. M. Lee, J. S. Ahn, and B. S. Kim. 2007. Cloning, sequencing, and characterization of the pradimicin biosynthetic gene cluster of Actinomadura hibisca P157-2. J. Microbiol. Biotechnol. 17: 830-839   과학기술학회마을
51 Hong, J. S., S. H. Park, C. Y. Choi, J. K. Sohng, and Y. J. Yoon. 2004. New olivosyl derivatives of methymycin/ pikromycin from an engineered strain of Streptomyces venezuelae. FEMS Microbiol. Lett. 238: 391-399
52 Baltz, R. H. 2005. Antibiotic discovery from Actinomycetes: Will a renaissance follow the decline and fall? SIM News 55: 186-196
53 Subba, B., N. P. Kurumbang, Y. S. Jung, Y. J. Yoon, H. C. Lee, K. Liou, and J. K. Sohng. 2007. Production of aminoglycoside in non-aminoglycoside producing Streptomyces lividans. Bioorg. Med. Chem. Lett. 17: 1892-1896   DOI   ScienceOn
54 Kharel, M. K., D. B. Basnet, H. C. Lee, K. Liou, Y. H. Moon, J. J. Kim, J. S. Woo, and J. K. Sohng. 2004. Molecular cloning and characterization of a 2-deoxystreptamine biosynthetic gene cluster in gentamicin-producing Micromonospora echinospora ATCC 15835. Mol. Cells 18: 71-78
55 Poulsen, S. M., C. Kofoed, and B. Vester. 2000. Inhibition of the ribosomal peptidyl transferase reaction by the mycarose moiety of the antibiotics carbomycin, spiramycin and tylosin. J. Mol. Biol. 304: 471-481   DOI   ScienceOn
56 Borisova, S. A., L. Zhao, C. E. MelanconIII, C. L. Kao, and H. W. Liu. 2004. Characterization of the glycosyltransferase activity of DesVII: Analysis of and implications for the biosynthesis of macrolide antibiotics. J. Am. Chem. Soc. 126: 6534-6535   DOI   ScienceOn
57 Lee, S. K., J. W. Park, S. R. Park, J. S. Ahn, C. Y. Choi, and Y. J. Yoon. 2006. Hydroxylation of indole by PikC cytochrome P450 from Streptomyces venezuelae and engineering its catalytic activity by site-directed mutagenesis. J. Microbiol. Biotechnol. 16: 974-978   과학기술학회마을
58 Litovchick, A., A. Lapidot, M. Eisenstein, A. Kalinkovich, and G. Borkow. 2001. Neomycin B-arginine conjugate, a novel HIV-1 Tat antagonist: Synthesis and anti-HIV activities. Biochemistry 40: 15612-15623   DOI   ScienceOn
59 Kharel, M. K., B. Subba, D. B. Basnet, J. S. Woo, H. C. Lee, K. Liou, and J. K. Sohng. 2004. A gene cluster for biosynthesis of kanamycin from Streptomyces kanamyceticus: Comparison with gentamicin biosynthetic gene cluster. Arch. Biochem. Biophys. 429: 204-214   DOI   ScienceOn
60 Tsuchizaki, N., K. Ishino, F. Saito, J. Ishikawa, M. Nakajima, and K. Hotta. 2006. Trends of Arbekacin-resistant MRSA strains in Japanese hospitals (1979 to 2000). J. Antibiot. 59: 229-233   DOI   ScienceOn
61 Dax, S. L. 1997. Antibacterial Chemotherapeutic Agents, 1st Ed. Blackie Academic & Professional, London
62 Chen, H., M. G. Thomas, B. K. Hubbard, H. C. Losey, C. T. Walsh, and M. D. Burkart. 2000. Deoxysugars in glycopeptide antibiotics: Enzymatic synthesis of TDP-Lepivancosamine in chloroeremomycin biosynthesis. Proc. Natl. Acad. Sci. USA 97: 11942-11947
63 Hong, J. S., S. J. Park, N. Parajuli, S. R. Park, H. S. Koh, W. S. Jung, C. Y. Choi, and Y. J. Yoon. 2007. Functional analysis of desVIII homologues involved in glycosylation of macrolide antibiotics by interspecies complementation. Gene 386: 123-130   DOI
64 Zhao, X. Q., Y. Y. Jin, H. J. Kwon, Y. Y. Yang, and J. W. Suh. 2006. S-Adenosylmethionine (SAM) regulates antibiotic biosynthesis in Streptomyces spp. in a mode independent of its role as a methyl donor. J. Microbiol. Biotechnol. 16: 927- 932   과학기술학회마을
65 Elchert, B., J. Li, J. Wang, Y. Hui, R. Rai, R. Ptak, P. Ward, J. Y. Takemoto, M. Bensaci, and C. W. Chang. 2004. Application of the synthetic aminosugars for glycodiversification: Synthesis and antimicrobial studies of pyranmycin. J. Org. Chem. 69: 1513-1523   DOI   ScienceOn
66 Kim, K. P., C. S. Shin, S. J. Lee, J. H. Kim, J. M. Young, Y. Y. Lee, J. H. Ahn, J. W. Suh, and Y. G. Lim. 2006. Proteomes induced by S-adenosyl-L-methionine in Streptomyces coelicolor A3(2). J. Microbiol. Biotechnol. 16: 799-803   과학기술학회마을
67 Kudo, F., Y. Yamamoto, K. Yokoyama, T. Eguchi, and K. Kakinuma. 2005. Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773. J. Antibiot. 58: 766-774   DOI   ScienceOn
68 Weber, J. M., J. O. Leung, S. J. Swanson, K. B. Ikler, and J. B. McAlpine. 1991. An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea. Science 252: 114-117   DOI
69 Bannister, B. and B. A. Zapotocky. 1992. Protorubradirin, an antibiotic containing a C-nitroso-sugar fragment, is the true secondary metabolite produced by Streptomyces achromogenes var. rubradiris. J. Antibiotics 45: 1313-1324   DOI
70 Leadly, P. F. 1997. Combinatorial approaches to polyketide biosynthesis. Curr. Opin. Chem. Biol. 1: 335-341
71 Xue, Y., L. Zhao, H. W. Liu, and D. H. Sherman. 1998. A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: Architecture of metabolic diversity. Proc. Natl. Acad. Sci. USA 95: 12111-12116
72 Sohng, J. K., T. J. Oh, J. J. Lee, and C. G. Kim. 1997. Identification of a gene cluster of biosynthetic genes of rubradirin substructures in S. achromogenes var. rubradiris NRRL 3061. Mol. Cells 7: 674-681
73 Thuy, T. T., K. Liou, T. J. Oh, D. H. Kim, D. H. Nam, J. C. Yoo, and J. K. Sohng. 2007. Biosynthesis of dTDP-6-deoxybeta- D-allose, biochemical characterization of dTDP-4-keto- 6-deoxyglucose reductase (gerK1) from Streptomyces sp. KCTC 0041BP. Glycobiology 17: 119-126   DOI   ScienceOn
74 Zhang, G., J. Shen, H. Cheng, L. Zhu, L. Fang, S. Luo, M. T. Muller, G. E. Lee, L. Wei, Y. Du, D. Sun, and P. G. Wang. 2005. Syntheses and biological activities of rebeccamycin analogues with uncommon sugars. J. Med. Chem. 48: 2600- 2611   DOI   ScienceOn
75 Jaishy, B. P., S. K. Lim, I. D. Yoo, J. C. Yoo, J. K. Sohng, and D. H. Nam. 2006. Cloning and characterization of a gene cluster for the production of polyketide macrolide dihydrochalcomycin in Streptomyces sp. KCTC 0041BP. J. Microbiol. Biotechnol. 16: 764-770   과학기술학회마을
76 Solenberg, P. J., P. Matsushima, D. R. Stack, S. C. Wilkie, R. C. Thompson, and R. H. Baltz. 1997. Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces toyocaensis. Chem. Biol. 4: 195-202   DOI   ScienceOn
77 Sohng, J. K., H. J. Kim, D. H. Nam, D. O. Lim, J. M. Han, H. J. Lee, and J. C. Yoo. 2004. Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155. Biotechnol. Lett. 26: 185-191   DOI   ScienceOn
78 Kim, C. Y., H. J. Park, and E. S. Kim. 2006. Functional dissection of sigma-like domain in antibiotic regulatory gene, afsR2 in Streptomyces lividans. J. Microbiol. Biotechnol. 16: 1477-1480   과학기술학회마을