• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2002년도 추계학술대회초록집
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• pp.145-145
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• 2002

Chronic viral infection has been reported to cause a range of hepatic lesion, including hepatic fibrosis, cirrhosis and hepatocellular carcinoma (HCC) in a wide variety of animal species. Woodchucks (Marmota monax) chronically infected with woodchuck hepatitis virus (WHV) develop similar progressive hepatic inflammatory and neoplastic lesions that are remarkably similar to those associated with HBV infection in humans. Twenty two-month-old offspring from woodchucks (Marmota monax) experimentally infected with woodchuck hepatitis virus, were purchased. One randomly chosen animal was autopsied. The liver exhibits marked cirrhotic changes characteristic of the pre-transformation phase of WHV. We believe that this may represent a new suitable and cost-effective model for the disease processes associated with hepadnaviruses in a number of other species, most notably Hepatitis B virus infection in man.

• 한국임상수의학회지
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• 제4권2호
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• pp.463-472
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• 1987

Although there are many reports on the bovine electroacupuncture anesthesia, its application to clinical cases needs much to be verified. In order to apply the electroacupuncture anesthesia to bovine species, a new anesthesia technique, which is safe and effective, must be developed. In the present study, 11 meridian points were selected and 11 kinds of meridian points were prescribed to develop an effective and safe electroacupucture methods in the bovine species. The results obtained are summarized as fellows : 1. When the maximun electric current (30Hz at 5.3-5.6V ) was applied, anesthesia was attained with the signs of tetany and tremor : When the electric current was continued for 15-20 minutes, the signs of tetany and tremor diminished markedly. 2. If the electric current was adjusted to 30Hz at 3.5-4.0V and continued, the tetany and tremor disappeared. The skin was slackened, thus suitable for surgical operation. The continuation of anesthesia could be regulated at operator's own will. 3. The same anesthesia effect could be attained by using any one meridian point when the points were symmetrical. 4. Transient changes of clinical signs and blood pictures were noted during the stages of acupuncture anesthesia. However, the red blood cell counts, white blood cell counts and hematocrit values were always within the normal range.

• 오토저널
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• 제10권6호
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• pp.72-84
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• 1988

Engine performance is one of the main objectives specified at the beginning of a new engine design project. The cycle simulation for SI engine is based on the zero-dimensional gas exchange model and a heat release expression by Viebe. This program also requires minimum input data and takes only a short time to run. Heat transfer from cylinder transfer formula. The flow coefficient (effective area) is calculated from valve lift using the standard flow coefficient curve and engine friction is calculated from the Millington and Hartles' engine friction formula. The chemical species considered in burned gas are 6 species CO, CO, H$_{2}$, H$_{2}$O, $O_{2}$, N$_{2}$ and the cylinder pressure, homogeneous cylinder temperature, gas composition and burned fraction are calculated at each crank angle through the cycle. To check the validity and accuracy, experimental study was done with 3 engines for measuring cylinder pressure, indicated mean effective pressure, brake mean effective pressure and air flow rate, etc. Despite its simple assumptions, cycle simulation showes excellent breathing and performance correlation when compared with data of tested engines, and have been proved useful in engine design.

• Mycobiology
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• 제31권4호
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• pp.196-199
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• 2003

A very reliable and specific method for the identification of fungi in ectotrophic mycorrhizal symbiosis was developed using a specific PCR assay based on the amplification of the ITS1 region. To obtain specific data, an ITS-diagnostic assay was carried out that reveals genera and species specific sequences. Here, an application of one method is presented, which covers the identification of pure mycelia, basidiocarps as well as mixed samples such as ectomycorrhizal roots that were mingled with remains of the host plant. For this purpose a protocol was established that allowed the extraction of DNA from single mycorrhizal roots. In order to perform a specific ITS analysis we generated a new ITS-primer(ITS8) by a multiple alignment of five different genera and species of mycorrhizal fungi. The utilization of ITS1 and ITS8 resulted in specific PCR amplicons, which were characterized by sequencing without purification steps, even when the template DNA was associated with roots.

• 약학회지
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• 제42권2호
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• pp.132-139
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• 1998

Currently, cancer is the primary cause of death and 50% of cancer patients are incurable by surgery, radiotherapy and chemotherapy. Therefore, immunotherpy is interested as the fourth remedy. Biological response modifier (BRM), such as organometallic compounds, glycoproteins, polysaccharides and other natural products. Is the one which can enhance the immune response against cancer cell. To develop new BRM from natural sources, we investigated 63 species Korean traditional medicines by observing the mitogenic activity to splenocytes, generation of activated killer cells and activation of macrophages. Finally, we selected 9 species including Angelicae gigantis Radix, Mori Cortex Radicis, Arisaematis Tuber, Salviae Radix, Cnidii Rhizoma, Ligusti Fructus, Pasoraliae Semen, Loranthi Ramulus, Ginseng Radix. Bioassay-guided fractionation and purification is undergoing.

• 한국어류학회지
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• 제20권4호
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• pp.324-326
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• 2008

거북복과(Ostraciidae)에 속하는 Lactoria fornasini 1개체(표준체장 103.7 mm)가 제주도 위미에 설치된 정치망에 처음으로 채집되었다. 이 종은 눈앞에 1쌍의 가시가 돌출되었고, 꼬리지느러미 아래쪽의 배쪽 융기선으로부터 1쌍의 가시가 후방을 향해 돋아 있으며 등의 융기부 중앙에는 날카로운 가시를 갖고 있다. 그리고 몸에는 다양한 파란색 줄무늬를 가지고 있다. 이 미기록종의 신한국명을 "줄무늬뿔복"으로 명명하였다.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.357-369
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• 2010

Cymbidium is horticulturally important and has been one of the most commercially successful orchid plants as well as cut flowers around the world including Korea. Up to now, a huge number of elite Cymbidium cultivars have been released on the commercial market via cross-hybridization, mutation and polyploidization breeding techniques. To investigate on breeding system in Cymbidium, we inquired the brief history and techniques of breeding and the current status on Cymbidium breeding in Korea. Also, the general propagation process of elite Cymbidium lines via tissue culture should be presented. However, the slow process of conventional breeding and the lack of useful genes in Cymbidium species delays the introduction of new cultivars to the commercial market. To solve these limitations, efficient regeneration and genetic transformation systems should be established in the improvement of Cymbidium breeding program. During the last several decades, some progress has been made in tissue culture and genetic transformation in Cymbidium species. We review the recent status of tissue culture and genetic transformation systems in Cymbidium plants.

• 한국균학회지
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• 제47권3호
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• pp.187-197
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• 2019

Ascomycota is the largest phylum of the Fungi, including approximately 6,600 genera. They are often isolated from soils, indoor air, and freshwater environments, but also from plants as pathogens or endophytes. In this study, four species of Ascomycota (two of Cladosporium and one of each Daldinia and Nigrospora) were collected from the leaves of four woody plants (Camellia japonica, Ginkgo biloba, Quercus sp., Vitis vinifera). Their cultural characteristics were investigated on five different media (PDA, V8A, CMA, MEA, CZA) at 3 days after incubation at $25^{\circ}C$ in darkness. BLASTn search and phylogenetic analysis were performed using the internal transcribed spacer (ITS) rDNA sequences, in addition to tef1 gene sequences for Cladosporium species. Based on the cultural, morphological, and phylogenetic data, the isolates were identified as Cladosporium anthropophilum, Cladosporium pseudocladosporioides, Daldinia eschscholtzii, and Nigrospora chinensis. Previously, some members of Cladosporium and Nigrospora have been recorded as endophytes inhabiting the leaves and stems of various plants, whereas Daldinia eschscholtzii is a wood-inhabiting endophyte or wood-decaying fungus. To our knowledge, this is the first report of these four ascomycetes in Korea.

• 한국어류학회지
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• 제30권4호
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• pp.242-246
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• 2018

농어목 전갱이과에 속하는 Scomberoides lysan 2개체(표준체장 334, 354 mm)가 2010년 9월 제주도 서귀포시 대포동의 정치망에서 처음으로 채집되었다. 이 종은 최근 우리나라에 보고된 가시전갱이(S. tol)와 형태적으로 유사하지만 주둥이 뒤쪽 끝이 눈의 말단에 이르며, 체측에 5~8개의 작은 검은색 반점들이 두 줄로 나열되는 특징을 갖는다. 따라서 체측에 두 줄의 작은 반점을 가지는 형태적 특징에 근거해서 이 미기록종의 국명을 "두점줄가시전갱이"로 제안한다.

• 수산해양기술연구
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• 제57권4호
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• pp.374-381
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• 2021

A newly recorded chaetodontid species, Chaetodon speculum Cuvier, 1831 was collected in July, 2021 from Pohang, Korea. Its body color is yellow with black stripe across the eyes and a large spot occupying one fourth of the upper part of the body. C. speculum is very similar to congeneric species, C. zanzibarensis, but it can be distinguished by the size of the spot, shape of the lateral line, number of longitudinal line scales and geographic distribution. C. speculum is readily identified between post-larval stage and adult stage because of its similar external shape. Molecular analysis based on mitochondrial DNA COI sequences showed our specimen perfectly matched C. speculum. Therefore, we propose the new Korean name, "Heug-jeom-na-bi-go-gi" for C. speculum.