• YAKHAK HOEJI
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• v.40 no.6
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• pp.705-712
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• 1996

We have previously shown that determination of glucose uptake using ${\alpha}$-methylglucose(${\alpha}$-MG) is very sensitive and rapid parameter for the assessment of loss of cellular fu nction in renal cell line($LLC-PK_1$). The present study was designed to elucidate the mechanism of inhibition of ${\alpha}$-MG uptake and the intracellular site of toxic action of cisplatin(CIS). $LLC-PK_1$ cells were exposed to various concentrations(5 ${\mu}$M-l00 ${\mu}$M) of CIS for 5 hrs or 24 hrs and ${\alpha}$-MG uptake was determined. Mitochondrial function was evaluated by measuring intracellular ATP content and MTT reduction. The activities of marker enzymes for the basolateral membrane(Na$^+$-K$^+$ ATPase) and brush border membrane (alkaline phosphatase: ALP) were also measured. CIS treatment significantly inhibited the ${\alpha}$-MG uptake in a time- and dose-dependent manner above 25 ${\mu}$M for 5 hrs. Intracellular ATP content and MTT reduction were affected by 24 hr-treatment of 50 ${\mu}$M CIS. The activities of Na$^+$-K$^+$ ATPase and ALP were significantly decreased at 10 ${\mu}$M and 5 ${\mu}$M of CIS for 24 hrs, respectively. The incubation with CIS for 5 hrs had no effects on the intracellular ATP content, MTT reduction and the activities of marker enzymes up to 100 ${\mu}$M. These results partly indicate that inhibition of ${\alpha}$-MG uptake by CIS may not be attributed to the disturbance of mitochondrial function or inhibition of the activity of Na$^+$-K$^+$ ATPase and can be resulted from direct effect of CIS on the Na$^+$/glucose cotransporter in brush border membrane. This study shows that additional mechanistic information, indicating the intracellular site of nephrotoxic action, can be gained by coupling the ${\alpha}$-MG uptake and ATP content or the activity of Na$^+$-K$^+$ ATPase.

• Journal of the Korean Society of Costume
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• v.48
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• pp.5-24
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• 1999

The globalization of Korean beauty comes partly from the succession of korean traditional culture and its modernization will be the one of very important issues in 21st century. This paper was done in order to analyze the process of Korean design development in modern Korean fashion in terms of the succession and modernization of Koreantraditional Beauty and based on this analysis also suggest new possibilities for successful globalization of Korean traditional beauty andbased on this analysis also suggest new possibilities for successful globalization of Korean fashion in 21st century. 227 fashion designs which represent typical elements of korean traditional costume were selected from fashion magazines books SFAA collection books and company brochures published from 1986, to 1997. Content analysis was used with 8 variables including 7 design elements(line shape, color, textile, motif, detail, accessories) and year. Results of analysis showed that the process of Korean Design development in modern Korean fashion has in the use of straight lines and simple silhouettes. Diversity could be found in the application of various kinds of outwear items as well as in the variety of materials and details. Symbolic tradtional motifs and accessories were used to enliven the Korean folklore image, These trends became more salient with the turning point of 1993 when a few designers began to participate in Paris Collection. For successful globalization designers should have more active attitudes toward exploiting the essence of Korean beauty and developing them as modern and global styles.

• Journal of the Korean Society for information Management
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• v.27 no.3
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• pp.67-82
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• 2010

In comparison to the services provided by the typical information systems, the historical records from the period before the modern times require additional information services to reflect the format and content oriented peculiarities of the data. The additional services also need to be considered in light of the users' information needs. For this study, a series of literature reviews was conducted to present the specific analysis criteria for the historical information services. Based on these criteria, the domestic and foreign historical information service provision and use status was investigated. In addition, users' information needs were identified and user-based historical information service improvement suggestions were generated.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.440-447
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• 2003

Up until today, the key to contouring has been resumed in these two alternatives, either limiting the adipocyte storing capacity by modulating lipogenesis, or by stimulating lipolysis to eliminate adipocyte lipid content. Another interesting way could be the regulation of adipocyte differentiation. In this work, we have evaluated the effect of a brown algal extract of Sphacelaria scoparia (SSE) on the differentiation of pre-adipocytes into adipocytes. A pre-adipocyte line (3T3-L 1) was used. The differentiation was evaluated by the measure of produced lipids thanks to red oil coloration and spectrophotometry, and also by the expression of adipocyte differentiation markers: enzymes such as fatty acid synthase (FAS) and stearoyl CoA desaturase (SCD), or membrane proteins such as glucose transporters (GLUT -4) and fatty acid transporters (FAT) expressed on the surface of human adipocytes. These genes are under control of two transcription factors: CAAT-enhancer binding protein (c/EBP alpha) and sterol response element binding protein (SREBP1). All these markers were analysed at different stages of differentiation by RT -PCR. Sphacelaria extract (SSE) inhibits pre-adipocytes differentiating into adipocytes following a dose-dependant relation, using a kinetics similar to retinoic acid. It decreases the expression of mRNA specific to FAS, FAT, GLUT -4, SCD1, c/EBP alpha and SREBP1. Moreover, SSE regulated on collagen 1 and collagen 4 expression. A stimulation of collagen 1 was also measured in human skin fibroblasts. Thus, SSE performs as a genuine differentiation inhibitor and not only as a lipogenesis inhibitor, and could be used in slimming products.

• BMB Reports
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• v.40 no.6
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• pp.944-951
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• 2007

3-Hydrogenkwadaphnin (3-HK) is a daphnane-type diterpene ester isolated from Dendrostellera lessertii (Thymelaeaceae) with high differentiation and apoptotic potency in leukemic cells without any measurable adverse effects on normal cells (Moosavi et al., 2005b). In this study, we report that 3-HK (12 nM) has the ability to cease proliferation, induce differentiation and apoptosis in chronic myelogenous leukemia (CML) K562 cell line. The treated cells lost erythroid properties and differentiated along the megakaryocytic lineage based on the morphological features apparent after Wright-Giemsa staining, DNA content analysis and the expression of cell surface marker glycoprotein IIb as analyzed by flow cytometry. Moreover, using Hoechst 33258 and Annexin V double staining indicated the occurrence of apoptosis among the treated cells. On the other hand, restoration of the depleted GTP pool size by exogenous addition of guanosine ($50{\mu}M$) reduced the effect of the drug regarding the extent of differentiation while no further enhancement of 3-HK effect was obtained by addition of exogenous hypoxanthine ($100{\mu}M$). These interesting results necessitate further investigation regarding the mechanism of action of this unique anti-leukemic agent.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.337-342
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• 2021

The purpose of this study is to analyze the environment and strategic behaviors of cultural contents companies with a focus on Iconix, and to derive strategic recommendations for Iconix to pursue in order to create a sustainable competitive advantage. As a result of the analysis, Iconix is a vertically integrated development-business system from content planning to business in line with their mission to develop into an all-weather entertainment content provider that can confidently compete with the major players in the US and Europe that are already leading the global market. It is building a strong global business network covering both domestic and overseas markets in stages, taking a high-level global strategy. However, depending on Pororo's success or due to various problems within the organizational structure, it is facing limitations. Therefore, if the various strategic suggestions presented in this study are implemented based on the One Source Multi Channel/Multi Use strategy that can maximize the added value of contents through the participation and business linkage of leading companies in each sector of the entertainment industry, the total entertainment will be stabilized. It will establish itself as a leader in the contents industry.

• Korean Journal of Remote Sensing
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• v.24 no.6
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• pp.551-558
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• 2008

It is well known that the protein content of rice grain is an indicator of taste of cooked rice in the countries where people as the staple food. Ground-based optical sensing over the crop canopy would provide information not only on the mass of plant body which reflects the light, but also on the crop nitrogen content which is closely related to the greenness of plant leaves. The vegetation index has been related to crop variables such as biomass, leaf nitrogen, plant cover, and chlorophyll in cereals. The objective of this study was to investigate the correlation between GNDVI and NDVI values, and grain protein content at different dates and to estimate the grain protein content using G(NDVI) values. We measured Green normalized difference vegetation index [$GNDVI=({\rho}0.80{\mu}m-{\rho}0.55{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.55{\mu}m)$] and [$GNDVI=({\rho}0.80{\mu}m-{\rho}0.68{\mu}m)/({\rho}0.80{\mu}m+{\rho}0.68{\mu}m)$] by using two different active sensors. The study was conducted during the rice growing season for three years from 2005 through 2007 at the experimental plots of National Institute of Agricultural Science and Technology. The experiments were carried out by randomized complete block design with the application of four levels of nitrogen fertilizers(0, 70, 100, 130kg N/ha) and the same amount of phosphorous and potassium content of the fertilizers. After heading stage, relationships between GNDVI of rice canopy and grain protein content showed the highly positive correlation at different dates for three years. GNDVI values showed higher correlation coefficients than that of NDVI during growing season in 2005-07. The correlation between GNDVI values at different dates and grain protein contents was highly correlated at early July. We attempted to estimate the grain protein content at harvesting stage using GNDVI values from early July for three years. The determination coefficients of the linear model by GNDVI values were 0.9l and the measured and estimated grain protein content at harvesting stage using GNDVI values highly correlated($R^2=0.96^{***}$). Results from this study show that GNDVI appeared very effective to estimate leaf nitrogen and grain protein content of rice canopy.

• Publications of The Korean Astronomical Society
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• v.27 no.4
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• pp.243-248
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• 2012

Nearby spiral galaxies M101 and M81 are considered to have undergone a galaxy-galaxy interaction. M101 has experienced HI gas infall due to the interaction. With AKARI far-infrared (IR) photometric observations, we found regions with enhanced star forming activity, which are spatially close to regions affected by the interaction. In addition, the relation between the star formation rate (SFR) and the gas content for such regions shows a significant difference from typical spiral arm regions. We discuss possible explanations for star formation processes on a kiloparsec scale and the association with interaction-triggered star formation. We also observed the compact group of galaxies Stephan's Quintet (SQ) with the AKARI Far-infrared Surveyor (FIS). The SQ shows diffuse intergalactic medium (IGM) due to multiple collisions between the member galaxies and the IGM. The intruder galaxy NGC 7318b is currently colliding with the IGM and causes a large-scale shock. The 160 micron image clearly shows the structure along the shock ridge as seen in warm molecular hydrogen line emission and X-ray emission. The far-IR emission from the shocked region comes from the luminous [CII]$158{\mu}m$ line and cold dust (~ 20 K) that coexist with molecular hydrogen gas. Survival of dust grains is indispensable to form molecular hydrogen gas within the collision age (~ 5 Myr). At the stage of the dusty IGM environment, [CII] and $H_2$ lines rather than X-ray emission are powerful cooling channels to release the collision energy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.5
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• pp.390-394
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• 2002

Spikelet number per unit area(SPN) is a major determinant of rice yield. Nitrogen nutrition status and biomass during reproductive stage determine the SPN. To formulate a model for estimating SPN, the 93 field experiment data collected from widely different regions with different japonica varieties in Korea and Japan were analyzed for the upper boundary lines of SPN responses to nitrogen nutrition index(NNI), shoot dry weight and shoot nitrogen content at panicle initiation and heading stage. The boundary lines of SPN showed asymptotic responses to all the above parameters(X) and were well fitted to the exponential function of $f(X)=alphacdot{1-etacdotexp(gamma;cdot;X)}$. Excluding the constant, from the boundary line equation, the values of the equation range from 0 to 1 and represent the indices of parameters expressing the degree of influence on SPN. In addition to those indices, the index of shoot dry weight increase during reproductive stage was calculated by directly dividing the shoot dry weight increase by the maximum value ($800 extrm{g/m}^{-2}$) of dry weight increase as it showed linear relationship with SPN. Four indices selected by forward stepwise regression at the stay level of 0.05 were those for NNI ($I_{NNI}_P$) at panicle initiation, NNI($I_{NNI}_h$) and shoot dry weight($I_{DW}_h$) at heading stage, and dry weight increase($I_{DW}$) between those two stages. The following model was obtained: SPN=48683ㆍ $I_{DWH}$$^{0.482}$ㆍ $I_{NNIp}$$^{0.387}$ㆍ $I_{NNIH}$$^{0.318}$ㆍ $I_{DW}$ $^{0.35}$). This model accounted for about 89% of the variation of spikelet number. In conclusion this model could be used for estimating the spikelet number of japonica rice with some confidence in widely different regions and thus, integrated into a rice growth model as a component model for spikelet number estimation.n.n.

• Current Research on Agriculture and Life Sciences
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• v.10
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• pp.117-123
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• 1992

Twenty and 24 lines selected from Korean local cultivar, Kalmi and Punggak, respectively, were grown in the University experiment farm to select promising lines for recurrent parent in a backcross breeding program for incorporation of resistance to Phytophthora capsici and Xanthomonas campestris pv vesicatoria into the Korean local cultivars. All the selections of 'Kalmi' and 'Punggak' were relatively late in maturity and the range of the variation was wider in the selections of 'Kalmi' than in those of 'Punggak'. Many of the selections from 'Kalmi' were vigorous, high in sugar content, and tolerant to virus in the field. Many of the selections from 'Punggak' were bearing large fruits with thick pericarp and high milling percentage. Line 6, 9, 14, 18 of 'Kalmi' and line 14, 16, 18 of 'Punggak' were selected as promising lines for recurrent parent in the backcross program.