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Safety Assessment of Oriental Medicines and Their Preparations (한약 및 한약제제의 안전성 평가)

  • Jeong, Il-Hyung;Kim, Jong-Hwa;Jeon, Jong-Sup;Cho, Sang-Hun;Park, Shin-Hee;Jo, Hyun-Ye;Kim, Young-Sug
    • Journal of Food Hygiene and Safety
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    • v.25 no.3
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    • pp.232-237
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    • 2010
  • This study was carried out to evaluate the safety of circulated oriental medicines and their related preparations in Gyeonggi-do. Total 366 samples (165 species) were analyzed about heavy metals, residual contents of sulfur dioxide ($SO_2$), and 68 samples were analyzed about preservatives. 17 samples (13 species, 4.6%) were exceeded the legal limit of heavy metal. The concentrations over the legal limit for Pb, Cd, As and Hg were 6.1~19.2 mg/kg, 0.4~0.7 mg/kg, 6.9 mg/kg and 0.7 mg/kg, respectively. In particular, the exceeding ratio (5.9%) of the legal limit of heavy metals in foreign products was 3.3 times more than domestic products (1.8%). 13 samples (10 species, 3.6%) exceeded the limit of residual sulfur dioxide and the concentration ranges were 105 to 428 mg/kg in domestic products, on the other hand foreign products were from 114 to 2,468 mg/kg. The mean concentration over the limit of residual sulfur dioxide of foreign products (804 mg/kg) was 2.4 times more than domestic products (338 mg/kg). In studying of the preservatives in oriental preparation, the contents of dehydroacetic acid (48.9~64.1%) in 3 samples of labeled solutions were under the labeled preservative contents and the contents of benzoic acid (139.9%) in 1 sample of labeled pill product was exceeded the labeled preservative contents. The numbers of the detected preservatives in unlabeled solutions, pills and granules were 4, 11 and 7, respectively.

Survey for contamination and study for reduction of ochratoxin A and aflatoxin in red pepper (고추 중 오크라톡신 A와 아플라톡신의 오염도 조사 및 저감화방안 연구)

  • Kim, Dong-Ho;Jang, Han-Sub;Kim, Yeong-Min;Ahn, Jong-Sung
    • Journal of Food Hygiene and Safety
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    • v.24 no.4
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    • pp.299-306
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    • 2009
  • Aflatoxin (AF) and Ochratoxin A (OTA) are carcinogenic and possible carcinogenic mycotoxins respectively produced by Aspergillus spp or Penicillium spp. The study for contamination survey and proposal for reduction of mycotoxin in red pepper were carried out. 192 samples were collected at such various stages and markets as pre/post-harvest stages, internet shopping mall /super-market and small stakeholder mill/geographically indicated company. As only 2 samples were positive for aflatoxin, so contamination rate was 1.04%. In the meanwhile, contamination rate for ochratoxin A was 21.88% and a various amount of OTA was detected in 42 positive samples. 6 samples were found to be contaminated at higher level than $5\;{\mu}gkg^{-1}$ for ochratoxin A, which was established recently as a maximum permissible limit in korea. There was no difference in degree of contamination with regard to cultivation type because any mycotoxin was not found at all in both organically and conventionally grown red pepper. But, there was statistically significant difference in the process of manufacturing. Finished products were OTA-contaminated at a level of $2.32\;{\pm}\;6.54\;{\mu}gkg^{-1}$(mean ${\pm}$ SD), even though OTA was not detected in deep frozen red peppers right after long term storage. And contamination for OTA was a level of $0.33\;{\pm}\;0.91\;{\mu}gkg^{-1}$(mean ${\pm}$ SD) in red paprika powder after uv sterilization, while the contamination for OTA was $2.78\;{\pm}\;4.49\;{\mu}kg^{-1}$(mean ${\pm}$ SD) in non-uv sterilized powder. In addition, our investigation shows that higher OTA contamination occurred in some of famous brand products sold in super-market and domestic products than products collected through on-line shopping or from small stakeholder mills and imported products respectively, however, difference was not statistically significant.

Quality Determination of Different Wholesale Cuts of Goat Carcass at Different Ages

  • Islam, R.;Rahman, S.M.E.;Khan, M.;Akhter, S.;Hossain, M.M.;Ding, Tian;Kim, Jai-Moung;Oh, Deog-Hwan
    • Journal of Food Hygiene and Safety
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    • v.25 no.3
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    • pp.251-257
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    • 2010
  • The experiment was conducted to find out the nutritive value and microbial status of different wholesale cuts of goat carcass. The meat sample (4 cuts from each animal $\times$ 3 different age groups $\times$ 3 animals in each group = 36 samples) was obtained from 1-, 2-, and 3-year aged goats. The whole sale cuts were shoulder, rack, loin and leg of each goat carcass. To assess the quality of meat sample, the general appearance, color, smell, juiciness, proximate composition, pH, total bacteria, coliform bacteria, and yeast were studied. The mean pH value of different cuts ranges from 5.65-5.69 didn't differ significantly, but due to age differences the pH values (5.59-5.74) differed significantly (p < 0.01). The values of juiciness in different ages ranged from 32.24-42.10% which differed significantly (p < 0.01). The marbling of the cuts of rack portion was more pronounced than that of other cuts. The ranges of crude protein (CP) content of goat carcass (20.78-27.71%) differed significantly (p < 0.01) and leg portion contained higher CP than other portion. Fat contents of different cuts ranged from 2.66-11.47% differed significantly (P < 0.01). The moisture content of the carcass differed significantly which ranged from 69.20-73.31%. The ash content of the cuts of 1-year aged groups (0.99 $\pm$ 0.13%) was higher than that of other age groups and differed significantly (P < 0.01). The calcium (Ca) content did not differ significantly. The phosphorus (P) content was higher in one year old goat (0.15 $\pm$ 0.03%) than that of the goats of other ages. The total viable count (TVC) content of microorganisms ranging from 5.05-5.15 log cfu/g at different ages did not differ significantly. The coliform count (CC) of different cuts differed significantly (P < 0.01) which ranged from 2.56-3.05 log cfu/g; it also differed significantly (P < 0.05) in different ages (2.79-2.84 log cfu/g) and was higher in 1 year old goat carcass. The yeast count differed significantly in different cuts (P < 0.01) and ages (P < 0.05). From the study it is concluded that the age and different wholesale cuts have direct influence on quality of goat carcass.

Behavior of amber fish, Seriola aureovittata released in the setnet (정치망내에 방류한 부시리, Seriola aureovittata 의 행동)

  • 신현옥;이주희
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.35 no.2
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    • pp.161-169
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    • 1999
  • This paper describes the swimming and escaping behavior of amber fish, Seriola aureovittata released in the first bag net of the setnet and observed with telemetry techniques. The setnet used in experiment is composed of a leader, a fish court with a flying net and two bag nets having ramp net. The behavior of the fish attached an ultrasonic depth pinger of 50 KHz is observed using a prototype LBL fish tracking system. The 3-D underwater position ofthe fish is calculated by hyperbolic method with three channels of receiver and the depth of pinger. The results obtained are as follows: 1. The fish released on the sea surface was escaped down to 15 m depth and rised up to near the sea surface during 5 minutes after release. The average swimming speed of the fish during this time was 0.87 m/sec. 2. The swimming speed of the fish is decreased slowly in relation to the time elapsed and the fish showed some escaping behavior forward to the fish court staying 1 to 7 m depth layer near the ramp net. The average speed of the fish during this time was 0.52 m/sec. 3. During 25 minutes after beginning of hauling net, the fish showed a faster swimming speed than before hauling and an escaping behavior repeatedly from the first ramp net to the second one in horizontal. In vertical, the fish moved up and down between the sea surface and 20 m depth. After this time, the fish showed the escaping behavior forward to fish court after come back to the first ramp net in spite of the hauling was continued. It is found that the fish was escaped from the first ramp net to the fish court while the hauling was carried out. The average speed of the fish after beginning of hauling was 0.72 m/sec which increased 38.5 % than right before the hauling and showed 0.44 to 0.82 m/see of speed till escaping the first bag net. The average swimming speed during observation was 0.67 m/sec (2.2 times of body length).

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A Study on the Telemetry System for the Inhabitant Environment and Distribution of Fish-III -Oxygen, pH, Turbidity and Distribution of Fishes- (어류의 서식환경과 분포생태의 원격계측에 관한 연구 -III -$용존산\cdot$pH 및 독도와 어류의 분포생태-)

  • 신형일;안영화;신현옥
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.35 no.2
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    • pp.136-146
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    • 1999
  • The telemetry system for the oxygen, pH, turbidity and the distribution ecology of fishes was constructed by the authors in order to product and manage effectively in shallow sea culture and setnets fisheries, and then the experiments for the telemetry system carried out at the culturing fishing ground in coast of Sanyang-Myon, Kyoungsangnam-Do and the set net fishing ground located Nungpo bay in Kojedo province respectively from October, 1997 to June 1998.As those results, the techniques suggested in the telemetry system for which find out the relationship between the physical and chemical environment in the sea and the distribution ecology of fishes gave full display its function, and its system could be operated as real time system. This research can also provide base-line data to develope a hybrid system unifying the marine environment information and the fisheries resources information in order to manage effectively coastal fishing ground.

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A Study on the Hull-dimension of 89 ton class Stow-net Vessel with Stern-fishing (89톤급 선미식 안강망어선의 선형치수에 관한 연구)

  • Park, Je-Ung;Lee, Hyeon-Sang
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.33 no.3
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    • pp.159-165
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    • 1997
  • This paper presents the optimum dimension of 89 ton class stow-net vessel with stern-fishing. The model of basic design is developed by using the optimization techniques referring to objective function and numerous constraints as follows; speed, fishing quantity, fishing days, catch per unit effort(CPUE), and weight/ratio of main dimensions, etc. Thus, the basic design of stow-net fishing vessel is built up by using the optimization of the design variables called the economic optimization criteria, and the objective function represents the criterion which is cost benefit ratio(CBR). The main conclusions are as follows. 1. S/W for decision of optimum hull size is developed in 89 ton class stow-net fishing vessel which is constructed with optimization of the design variables called the economic optimization criteria. 2. For optimum ship dimensions in 89 ton class stow-net fishing vessel, the hull dimensions can be obtained in the range of L= 27.3m, B = 6.6m, D = 2.80m, Cb = 0.695, T/D = 0.80, $\Delta$(displacement)=281.7ton with 10 knots.

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Vertical and horizontal distribution of squids in relation to oceanographic structure in the North Pacific Ocean (북태평양 오징어류의 연직 및 수평분포)

  • 김영승;이주희;박영철;황선재;김두남
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.35 no.2
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    • pp.102-117
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    • 1999
  • Vertical distribution of squids in relation to oceanographic structure was analyzed on the basis of experimental squid hand jigging fishing by R/V Pusan 851 in the area of 34°∼47° N, 150° E∼170°W in the North Pacific in summer from 1987 through 1993 with exception of 1991. The 6 species of squids showed different patterns of vertical and horizontal distribution as following; Boreopacific gonate squid (Gonatopsis borealis) were mainly caught in the layer of 71-80m fishing depth of the Subarctic Domain with water temperature of 6∼11℃ and salinity of 32.2∼33.6‰ and distributed in the latitudes of 41°∼43° N. Boreal clubhook squid (Onychoteuthis borealijaponica) were mainly caught in the layer of 11∼20m fishing depth of the Subarctic Domain with water temperature of 10∼12℃ and salinity of 32.9∼33.6‰ and distributed in the latitudes of 41°∼42N°. Tapanese flying squid (Todarodes pacificus) were mainly caught in the layer of 11∼20m fishing depth of the Transition Zone and the Subtropical Domain with water temperature of 15∼18℃ and salinity of 33.6∼34.0‰ and distributed in the latitudes of 40°∼42°N. Neon flying squid (Ommastrephes bartrami) were mainly caught in the layer of surface∼10m fishing depth of the Subarctic Convergence Zone and the Transition Zone with water temperature of 16∼17℃ and salinity of 33.7∼34.4‰ and distributed in the latitudes of 39 °∼41°N. Luminous flying squid (Symplectoteuthis luminosa) were mainly caught in the layer of 11 20m fishing depth of the Transition Zone and the Subtropical Domain with water temperature of 18∼20℃ and salinity of 33.8∼34.6‰ and distributed in the latitudes of 37°∼39°N. Purpleback flying squid (Symplectoteuthis oualaniensis) were mainly caught in the layer of surface∼10m fishing depth of the Subtropical Domain with water temperature of 24∼25℃ and salinity of 34.2∼34.4‰ and distributed in the latitude of 36°∼37°N.

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Detection and Differentiation of Herpes Simplex Virus 1 and 2, and Varicella-Zoster Virus in Vesicle Fluid, Joint Fluid and Serum using PCR Method (중합효소 연쇄반응에 의한 수포액, 혈액과 관절액에서 단순포진 바이러스 1, 2와 대상포진 바이러스의 검출과 감별)

  • Park, Hae-Kyung;Woo, So-Youn;Kim, Hyun-Jin;Lee, Chung-Hwa
    • The Journal of the Korean Society for Microbiology
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    • v.35 no.2
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    • pp.191-201
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    • 2000
  • The viruses of Herpes Simplex Virus 1 (HSV-1), Herpes Simplex Virus 2 (HSV-2) and Varicella-Zoster virus (VZV) which belong to the alpha herpes subfamily are important human pathogens. When eruptions were not fully developed from these viral infections, clinical diagnosis was not always easy and required virological confirmation test. The above viruses were reactivated in individuals who were compromised in immune competence for one reason or another. Polymerase chain reaction (PCR) enables rapid and sensitive detection of HSV and VZV DNAs. Its sensitivity was largely influenced by choice of primers. Authors conducted a study to detect of those three viruses in human specimens including vesicle fluid and joint fluid and serum using PCR methods. Primers used for this study were the general primer pair GPHV-RU which was known to amplify within the genes enjoying the highest degree of homology between UL15 of HSV and UL42 of VZV. PCR with primers hybridized pair GPHV-RU amplifies a 396 bp with THP-1 and HSV-2 standard strain DNA and 405 bp with VZV standard strain DNA. Restriction enzyme cleavage with HpaII and DdeI were used to detect and distinguish DNAs of THP-1 and HSV-2 and VZV. The purpose of this study was a rapid and easy detection of VZV and THP-1 or HSV-2 from various clinical specimens (vesicle fluid, serum and joint fluid) by PCR method. Used methods were: HSV PCR with primer 1, 2 and HpaII RE digestion; VZV nested PCR; HSV PCR with primer A, Band BssHII RE digestion. 1) In 33 cases (33/42, 78.6%) VZV was detected single or mixed infection from 42 clinical specimens which included vesicle fluid (5), serum form respiratory infected children (10), serum from immune suppressed adult cancer patients (7) and joint fluid from arthritis patients (20). 2) In 20 cases (20/42, 47.6%) HSV was detected singly or mixed infection and 19 of those cases were HSV-2 and 1 case was THP-1. 3) In 19 cases (19/42, 45.2%) VZV was singly detected which included serum from respiratory infected children (6 cases), joint fluid from arthritis patients (9 cases), vesicle fluid (2 cases) and serum form immunosuppressed cancer patients (2 cases). 4) HSV was singly detected in 6 cases (6/42, 14.3%) which included joint fluid from arthritis patients (5 cases) and serum form respiratory infected children (1 cases). 5) 14 cases of VZV and HSV mixed infection (14/42, 33.3%) were detected. They included vesicle fluid (3 cases), serum form immunosuppressed cancer patients (4 cases), serum from respiratory infected children (2 cases) and joint fluid from arthritis patients (5 cases). 6) HSV-1 and HSV-2 detection and typing by HSV PCR with primer A, Band BssHII RE digestion method was more sensitive and the results were easier to detect than on other method.

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Improving of the Fishing Gear and Development of the Automatic Operation System in the Anchovy Boat Seine-I -Underwater Geometry of the Model Net- (기선권현망어업의 어구 개량과 자동화 조업시스템 개발-I -시험어구의 수중형상-)

  • 장충식;김용해;안영수
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.36 no.4
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    • pp.299-308
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    • 2000
  • A model experiment on the anchovy boat seine was carried out in the southern sea of korea to analyze the vertical opening and the underwater geometry. The model net A was made of half size in the length and width of the prototype net. The model net B was attached floats and chain to the model net-A. The vertical opening and the underwater geometry of the model net were determined by distance of Minilog position with the combinations of the distance between paired boats and the towing speed. The results obtained can be summarized as follows; 1. Vertical opening of the model net was gradually lowered according to the increase of the distance between paired beats and the towing speed. 2. Vertical opening of Wing net, In side wing net, Square, Fore bag net, Flapper and After bag net of the model net A according to the distance between paired boats were varied in the range of 10.8~9.0, 12.0~8.3, 12.6~9.0, 10.4~6.6, 4.6~5.2, 8.8~7.7m respectively, varied in the range of 36~30, 21~15, 31~22, 80~51, 80~96, 59~51% of the normal opening respectively 3. Vertical opening of Wing net, In side wing net, Square, Fore bag net, Flapper and After bag net of the model net B according to the distance between paired boats were varied in the range of 9.1~8.5, 9.8~6.5, 11.2~8.0, 11.0~8.1, 4.7~5.0, 7.0~7.5m respectively, varied in the range of 30~28, 18~12, 27~20, 85~62, 87~93, 47~50% of the normal opening respectively 4. Vertical opening of each a part of the model net according to the towing speed was as same as the former. 5. Model net was appeared apparent the pocket shape, because Wing net and Inside wing net was opened 30% of the normal opening. 6. The bosom and the bag net of the model net A were risen up to the upper lazer, this phenomenon was more apparent as the distance between paired boats and the towing speed increase, but the model net B was almost constant. 7. Working depth of the model net was gradually hallowed according to the increase of the distance between paired boats and the towing speed

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Antioxidative Effect on the Green Tea and Puer Tea Extracts (녹차와 보이차 추출물의 항산화 효과)

  • Son Gyu-Mok;Bae Sung-Moon;Chung Ji-Young;Shin Dong-Joo;Sung Tae-Soo
    • The Korean Journal of Food And Nutrition
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    • v.18 no.3
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    • pp.219-224
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    • 2005
  • To see the antioxidative ability of puer tea against green tea, antioxidative effects of water and methanol extracts were compared and researched each. Water extracts from green tea and puer tea contain similar percentage of total polyphenol content while methanol extract from puer tea has about $58\%$ of total polyphenol content of that from green tea. Water extracts from both teas contain similar percentage of the electron donating ability while methanol extract from puer tea has about $54\%$ of the electron donating ability of that from green tea. Solvents made a big difference in SOD-like activities. The activations in water extracts were from $13.46\%$ to $48.93\%$, which didn't give much difference between green tea and puer tea. However, the activations in methanol extracts were $59.63\%$ in green tea and $48.93\%$ in puer tea, which was $3\~4$ times higher than that in water extracts and green tea activated better. The nitrite scavenging activity was similar in water extracts from both teas and the methanol extract from puer tea had a bit higher nitrite scavenging activity than that from green tea. The ACE inhibitory activity in water extract from puer tea was about $38\%$ higher than that from green tea while the activity of methanol extract from green tea was higher than that from puer tea. These results indicate that the antioxidative ability of water extract from puer tea is similar to that from green tea but the ACE inhibitory activity of puer tea reveals higher value and antioxidative ability of methanol extract from puer tea was lower than that from green tea while the nitrite scavenging activity of puer tea was higher.