• 한국축산식품학회지
• /
• 제20권3호
• /
• pp.207-213
• /
• 2000

계란에 함유되어 있는 영양소 함량을 좌우하는 주요한 요인은 사료적 요인에 의한 닭의 영양상태라 할 수 있으며, 이러한 사료적 변화에 현저하게 반응을 나타내는 것은 다수의 미량괄물질, 비타민, 불포화지방산 등이다. 본 실험에서는 지방산 강화란 16종, 비타민 강화란 4종 및 일반란 10종에 대해서 선분 함량을 분석하였으며, 지방사나 강화한 제품의 경우 16개사 제품에서 모두 DHA가 검출되었고, 6개사를 제외한 10개사의 제품에서 EPA가 검출되었다. DHA 함량은 두 개사 제품에서 69.84 mg, 46.16 mg을 나타낸 반면 대부분의 제품에서 100~300 mg 미만의 DHA 함량을 나타내었으며, $\omega$6/$\omega$3비율은 16개사 제품 모두 다른 비율을 나타내었다. 비타민 강화란은 비타민 A와 E를 동시 분리, 정량하였으며 비타민 A와 E 함량은 4개사 제품에서 유의차가 나타나지 않았으며, 일반란과의 비교에서도 큰 차이를 나타내지 않았다. 요오드 강화란 및 사포니 강화란과 비교해서 유의적으로 높은 함량을 나타내었다.(P<0.05) 이상의 결과에서 특수란의 특수성분 함량은 각 브랜드별로 다양한 분포를 보였으며 특히 비타민 강화란의 경우 일반란과의 차별성이 크게 나타나지 않았다. 이는 특수란 제품에 대한 소비자의 신뢰도를 저하시킬 수 있어 앞으로 이에 대해 관리 및 대책이 필요한 것으로 사료된다.

• Journal of Cancer Prevention
• /
• 제22권1호
• /
• pp.1-5
• /
• 2017

Pancreatic cancer is a highly aggressive malignant tumor of the digestive system and radical resection, which is available to very few patients, might be the only possibility for cure. Since therapeutic choices are limited at the advanced stage, prevention is more important for reducing incidence in high-risk individuals with family history of pancreatic cancer. Epidemiological studies have shown that a high consumption of fish oil or ${\omega}3-polyunsaturated$ fatty acids reduces the risk of pancreatic cancers. Dietary fish oil supplementation has shown to suppress pancreatic cancer development in animal models. Previous experimental studies revealed that several hallmarks of cancer involved in the pathogenesis of pancreatic cancer, such as the resistance to apoptosis, hyper-proliferation with abnormal $Wnt/{\beta}-catenin$ signaling, expression of pro-angiogenic growth factors, and invasion. Docosahexaenoic acid (DHA) is a ${\omega}3-polyunsaturated$ fatty acid and rich in cold oceanic fish oil. DHA shows anti-cancer activity by inducing oxidative stress and apoptosis, inhibiting $Wnt/{\beta}-catenin$ signaling, and decreasing extracellular matrix degradation and expression of pro-angiogenic factors in pancreatic cancer cells. This review will summarize anti-cancer mechanism of DHA in pancreatic carcinogenesis based on the recent studies.

• BMB Reports
• /
• 제35권6호
• /
• pp.547-552
• /
• 2002

The aim of our study was to investigate the protective effects of intraperitoneally-administrated vitamin E, dl-alpha lipoic acid, and linalool on the level of total lipid and fatty acid in guinea pig brains with oxidative stress that was induced by $H_2O_2$. The total brain lipid content in the $H_2O_2$ group decreased when compared to the $H_2O_2$ + vitamin E (p<0.05), $H_2O_2$ + linalool (p<0.05), ALA (p<0.05), control (p<0.01), linalool (p<0.01), and vitamin E (p<0.01) groups. While the proportion of total saturated fatty acid (${\Sigma}SFA$) in the $H_2O_2$ group significantly increased (p<0.005) when compared to the vitamin E group, it only slightly increased (p<0.01) when compared to the control and $H_2O_2$ + vitamin E groups. The ratio of the total unsaturated fatty acid (${\Sigma}USFA$) in the $H_2O_2$ groups was lower (p<0.05) than the control, vitamin E, and $H_2O_2$ + vitamin E groups. The level of the total polyunsaturated fatty acid (${\Sigma}PUEA$) in the $H_2O_2$ group decreased in when compared to the control, vitamin E, and $H_2O_2$ + vitamin E groups. While the proportion of the total w3 (omega 3), w6 (omega 6), and PUFA were found to be lowest in the $H_2O_2$ group, they were slightly increased (p<0.05) in the lipoic acid group when compared to the control and $H_2O_2$ + lipoic acid groups. However, the level of ${\Sigma}SFA$ in the $H_2O_2$ group was highest; the level of ${\Sigma}USFA$ in same group was lowest. As the proportion of ${\Sigma}USFA$ and ${\Sigma}PUFA$ were found to be highest in the linalool group, they were decreased in the $H_2O_2$ group when compared to the control group. Our results show that linalool has antioxidant properties, much the same as vitamin E and lipoic acid, to prevent lipid peroxidation. Additionally, vitamin E, lipoic acid, and linalool could lead to therapeutic approaches for limiting damage from oxidation reaction in unsaturated fatty acids, as well as for complementing existing therapy for the treatment of complications of oxidative damage.

• 한국식품저장유통학회지
• /
• 제12권2호
• /
• pp.117-122
• /
• 2005

미역이 산란계의 계란 품질에 미치는 영향을 조사하기 위해 기존 일반 양계사료(대조구)에 미역을 $5\%(A)$, $10\%(B)$ 및 $15\%(C)$ 첨가하여 생산한 계란의 일반성분, 무게, 색깔, 무기질, 총콜레스테롤 함량 및 지방산 조성을 분석한 결과는 다음과 같다. 미역함량을 증가시킴에 따라서, 일반성분은 수분 함량의 감소를 제외한 나머지 성분은 거의 변화가 없었다. 계란의 무게는 점차 감소하는 경향이었는데, 특히 난백에서 크게 감소하는 경향이었다. 난황 및 난백의 색깔은 검붉은 색으로 변화하는 경향이고, 특히 난황에서의 색깔 변화는 명확하고 유의 차가 인정되었다. 칼슘 함량은 $266.5\;mg\%$에서 $286.6\;mg\%$로, 철 함량은 $4,1\;mg\%$에서 $7.2\;mg\%$로, 칼륨 함량은 $360.5\;mg\%$에서 $430.6\;mg\%$로 각각 증가하였으나, 인의 함량은 $187.5\;mg\%$에서 $134.2\;mg\%$로 오히려 감소하는 경향이다. 총 콜레스테롤 함량은 $1828.3\~1850.2\;mg\%$로 거의 변화가 없었다. 주요 지방산은 palmitic acid, oleic acid, linoleic acid이고, $\omega6$ 지방산은 $0.9\%$에서 $2.5\%$로, $\omega6$ 지방산은 $14.2\%$에서 $17.6\%$로, 그리고 필수지방산 함량은 $14.5\%$에서 $18.4\%$로 증가하는 경향이었다.

• ALGAE
• /
• 제27권3호
• /
• pp.197-203
• /
• 2012

A eukaryotic microalga, Asterarcys quadricellulare KNUA020, was isolated from garden soil at Kyungpook National University in Daegu, South Korea and its biotechnological potential was assessed. Optimal growth was obtained when the culture was incubated at $25^{\circ}C$ and around pH 7.0. The total lipid content of the isolate was 15.5% of dry weight and its most abundant fatty acid was nutritionally important C18:3 ${\omega}3$ (${\alpha}$-linolenic acid, ALA). In addition, a high-value fatty alcohol, hexadecenol ($C_{20}H_{40}O$), was also identified in this photosynthetic microorganism. Hence, A. quadricellulare KNUA020 appears to be promising for use in the production of microalgae-based biochemicals.

• 생명과학회지
• /
• 제27권9호
• /
• pp.1020-1030
• /
• 2017

대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 오메가-3를 많이 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 오메가-3는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA의 항침윤, 항혈관 신생 및 항종양 형성능 억제의 기전을 규명하여 다음과 같은 결과를 얻었다. DHA는 인체 대장암 세포주 HT29 의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었다. FACS 분석에서 DHA 처리했을 때 Sub G1 phase의 세포가 DHA의 농도 의존적으로 증가 하였다. DHA 처리 후 cleaved PARP가 증가하고, uncelaved caspase-3가 감소 하였다. HT29 세포의 침윤능은 DHA 처리에 의해 억제 되었다. DHA 처리 후 MMP-9 및 MMP-2 mRNA양이 감소 되었을 뿐만 아니라 그 promoter의 reporter 활성도 억제하였으며 VEGF promoter 활성도 DHA에 의해 억제 되었다. NF-kB promoter 활성 및 핵으로의 이동도 DHA에 의해 억제 되었다. In vivo 동물실험에서 생쥐 대장암 세포주인 MCA38에 대한 Fat-1 transgenic mice에서의 종양 형성능은 현저히 억제 되었다. 면역형광염색법을 이용한 Fat-1 transgenic mice의 종양 조직에서의 TUNEL 양성세포는 wild type mice에 비해 현저히 증가하였으나 CD31의 형광강도는 감소 되었다. 이상의 결과로 오메가-3는 대장암 세포에서 NF-kB 억제에 따른 COX-2, MMP-2 및 MMP-9 등 matrix matalloproteinase의 억제를 통한 침윤능의 억제, VEGF 억제를 통한 혈관신생의 억제등 복합적 기전에 의해 항암작용을 나타내리라 생각되며, 따라서 오메가-3는 대장암의 예방 및 치료에 유용하게 사용될 수 있으리라 생각된다.

• ALGAE
• /
• 제37권2호
• /
• pp.175-183
• /
• 2022

Polar microorganisms produce physiologically active substances to adapt to harsh environments, and these substances can be used as biomedical compounds. The green microalga Micractinium variabile KSF0031, which was isolated from Antarctica, produced phytol, a natural antimicrobial agent. Furthermore, several polyunsaturated fatty acids (PUFAs), including omega-3, exhibit antioxidant properties. Here statistical methods (Plackett-Burman design and Box-Behnken design) were used to optimize the culture medium of KSF0031 to improve biomass production, and K₂HPO₄, MgSO₄·7H ₂O, and ammonium ferric citrate green (AFCg) were selected as significant components of the culture medium. Changes in the concentration of K₂HPO₄ and MgSO₄·7H ₂O as positive factors and AFCg as a negative factor affected cell growth to a remarkable degree. The biomass production in a 100 L culture using the optimized medium for 24 d at 18℃ was improved by 37.5% compared to that obtained using the original BG-11 medium. The quantities of PUFAs and phytol obtained were 13 mg g^-1 dry cell weight (DCW) and 10.98 mg g^-1 DCW, which represent improved yields of 11.70% and 48.78%, respectively. The results of this study could contribute to an improved production of phytol and fatty acids from Antarctic microalgae in the biomedical industry.

• Journal of Nutrition and Health
• /
• 제32권5호
• /
• pp.598-607
• /
• 1999

The purpose of this study was to develop a software system for computer nutrition counseling based on food intake and level of exercise measurements. Various software programs were developed using Powerbuilder 5.0 and categorized according to their function: 1) inputting general data including age, sex, weight, height, degree of acitivity and exercise amount of individuals, 2) inputting food intake based upon a 24-hour dietary recall method, 3) calculating energy and general nutrient intake and evaluating dietary status with respect to the Korean recommended dietary allowances, 4) calculating dietary intake of fatty acids, 5) calculating PUFA : MUFA : SFA and $\omega$6 :$\omega$3 ratios, 6) reporting the results of nutrient analysis, and 7) assessing the nutritional status of individuals and practicing nutrition education. This study provides various information on the assessment of nutritional status.

• 한국동물생명공학회지
• /
• 제34권2호
• /
• pp.75-85
• /
• 2019

Omega-3 α-linolenic acid and omega-6 linoleic acid are essential fatty acids for health maintenance of human and animals because they are not synthesized in vivo. The purpose of this study was to evaluate the effect of α-linolenic acid and linoleic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of α-linolenic acid and linoleic acid were added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, oocyte nuclear-maturation rate, blastocyst rate, blastocyst quality, and levels of prostaglandin E₂, 17β-estradiol, and progesterone in the spent medium. High doses (100 μM) of α-linolenic acid and linoleic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E₂ synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 μM α-linolenic acid and 10 μM linoleic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17β-estradiol / progesterone also significantly increased compared with control group (3.59 ± 0.22 vs. 2.97 ± 0.22, 3.4 ± 0.28 vs. 2.81 ± 0.19, respectively; p < 0.05). Our results indicated that supplementation with appropriate levels of α-linolenic acid and linoleic acid beneficially affects the change of hormone synthesis (in particular, an appropriate increase in the 17β-estradiol / progesterone synthesis ratio) for controlling oocyte maturation, leading to improved embryo quality. However, high doses of α-linolenic acid and linoleic acid treatment results in detrimental effects.

• Animal Bioscience
• /
• 제36권9호
• /
• pp.1414-1425
• /
• 2023

Objective: Long chain n-3 polyunsaturated fatty acids (PUFA) exert positive effects on human health. The long chain n-3 PUFA of pork can be increased by adding fish oil to the diet. Due to the cost and availability of fish oil an alternative source must be found. Methods: This study evaluated the effect of five dietary oils on meat quality, fatty acid composition and lipid stability. The five diets contained 1% palm oil (Control), 1% soya oil, 1% linseed oil, 1% fish oil, and 1% Echium oil, respectively. The trial consisted of 60 gilts, randomly allocated to five groups. Results: All color parameters, extractable fat content, fat free dry matter, and moisture content of the m. longissimus muscle were unaffected by dietary treatment. Consumers and a trained sensory panel could not detect a difference between the control samples and the Echium oil sample during sensory analysis. Samples containing higher levels of PUFA (soya, linseed, fish, and Echium oil) had higher levels of primary and secondary lipid oxidation products after refrigerated and frozen storage. However, these values were still well below the threshold value where off flavors can be detected. The Echium oil treatment had significantly higher levels of long chain PUFA than the linseed oil treatment, but it was still significantly lower than that of the fish oil treatment. Conclusion: Echium oil supplementation did not increase the levels of n-3 to the same extent as fish oil did. The result did however suggest that Echium oil can be used in pig diets to improve muscle long chain n-3 fatty acid content without any adverse effects on meat quality when compared to linseed, soya, and palm oil.