• 한국식품과학회지
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• 제30권6호
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• pp.1476-1479
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• 1998

키토산 올리고당이 ACE 저해활성과 SHR의 혈압에 미치는 영향을 검토하였다. 키토산 올리고당은 모두 ACE 저해활성을 나타내었다. ACE 저해활성$(IC_{50})$은 3량체가 $0.9\;{\mu}mole$로 가장 우수하였고 2량체의 경우에는 $2.4\;{\mu}mole$, 그 외의 올리고당은 모두 $>100\;{\mu}mole$였다. 강력한 ACE 저해제인 Captopril(2-D-mercaptopropanoyl-L-proline)의 인체 투여량을 기준으로 3량체 키토산 올리고당 2.14 mg/kg을 SHR에 강제 경구투여한 바, 4시간 경에 8주령 및 21주령 SHR모두 최저혈압을 보였고 이 때의 혈압 강하는 8주령 SHR $27{\pm}4.8\;mmHg$, 21주령 SHR $36{\pm}4.3\;mmHg$로 나타났다. 따라서 3량체 키토산 올리고당은 2량체 키토산 올리고당과 함께 향후 고혈압 치료제로서 응용가능함이 시사되었다.

• 한국수산과학회지
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• 제36권1호
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• pp.1-5
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• 2003

유기산을 이용한 알긴산의 분해 및 올리고당화 조건을 검색한 결과, 유기산 종류와 농도에 관계없이 $100^{\circ}C$ 이하의 온도 조건은 알긴산을 올리고당으로 분해하는데는 적절하지 못하였다. 반면, $110^{\circ}C$와 $120^{\circ}C$ 조건은 유기산 종류와 농도에 따라 다소의 차이는 있었으나 올리고당화할 수 있는 조건이었으며, 마이크로파 처리나 초음파 처리와 같은 물리적 가열 처리 방법은 알긴산 분해에 효과적인 방법이 아닌 것으로 확인되었다. 분해율의 측면에서 최적 분해 조건은 citrate나 malate로 $120^{\circ}C$에서 120분 가압가열처리하는 조건이었으나, TLC 상에서 $110^{\circ}C$에서는 $3\~4$개, $120^{\circ}C$ 처리 조건은 $7\~8$개의 올리고당 획분이 확인되었다. 이상의 결과로 미루어 보아 유기산을 이용한 알긴산 올리고당 생산은 $0.5\%$ citrate를 이용하여 $120^{\circ}C$에서 90분 동안 가압가열처리하는 것이 최적의 조건이었다.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.272-279
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• 2021

Two genes encoding probable α-ʟ-arabinofuranosidase (E.C. 3.2.1.55) isozymes (ABFs) with 92.3% amino acid sequence identity, ABF51A and ABF51B, were found from chromosomes 3 and 5 of Saccharomycopsis fibuligera KJJ81, an amylolytic yeast isolated from Korean wheat-based nuruk, respectively. Each open reading frame consists of 1,551 nucleotides and encodes a protein of 517 amino acids with the molecular mass of approximately 59 kDa. These isozymes share approximately 49% amino acid sequence identity with eukaryotic ABFs from filamentous fungi. The corresponding genes were cloned, functionally expressed, and purified from Escherichia coli. SfABF51A and SfABF51B showed the highest activities on p-nitrophenyl arabinofuranoside at 40~45℃ and pH 7.0 in sodium phosphate buffer and at 50℃ and pH 6.0 in sodium acetate buffer, respectively. These exoacting enzymes belonging to the glycoside hydrolase (GH) family 51 could hydrolyze arabinoxylo-oligosaccharides (AXOS) and arabino-oligosaccharides (AOS) to produce only ʟ-arabinose, whereas they could hardly degrade any polymeric substrates including arabinans and arabinoxylans. The detailed product analyses revealed that both SfABF51 isozymes can catalyze the versatile hydrolysis of α-(1,2)- and α-(1,3)-ʟ-arabinofuranosidic linkages of AXOS, and α-(1,2)-, α-(1,3)-, and α-(1,5)-linkages of linear and branched AOS. On the contrary, they have much lower activity against the α-(1,2)- and α-(1,3)-double-substituted substrates than the single-substituted ones. These hydrolases could potentially play important roles in the degradation and utilization of hemicellulosic biomass by S. fibuligera.

• Journal of Ginseng Research
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• 제46권2호
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• pp.290-295
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• 2022

Background: Dried and red ginseng are well-known types of processed ginseng and are widely used as healthy food. The dried and red ginseng quality may vary with the storage period of raw ginseng. Therefore, herein, the effect of the storage period of fresh ginseng on processed ginseng quality was evaluated through multicomponent quantification with statistical analysis. Methods: A method based on ultrahigh performance liquid chromatography coupled to triple quadrupole mass spectrometry in multiple-reaction monitoring mode (UPLC-MRM-MS) was developed for quantitation of ginsenosides and oligosaccharides in dried and red ginseng. Principal component analysis and partial least squares discriminant analysis were conducted to evaluate the dynamic distributions of ginsenosides and oligosaccharides after different storage periods. Results: Eighteen PPD, PPT and OLE ginsenosides and nine reducing and nonreducing oligosaccharides were identified and quantified. With storage period extension, the ginsenoside content in the processed ginseng increased slightly in the first 2 weeks and decreased gradually in the following 9 weeks. The content of reducing oligosaccharides decreased continuously as storage time extending, while that of the nonreducing oligosaccharides increased. Chemical conversions occurred during storage, based on which potential chemical markers for the storage period evaluation of fresh ginseng were screened. Conclusion: According to ginsenoside and oligosaccharide distributions, it was found that the optimal storage period was 2 weeks and that the storage period of fresh ginseng should not exceed 4 weeks at 0 ℃. This study provides deep insights into the quality control of processed ginseng and comprehensive factors for storage of raw ginseng.

• Animal Bioscience
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• 제36권6호
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• pp.891-898
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• 2023

Objective: This study aimed to determine the effect of crude oligosaccharide extract from coconut milk meal (CMM) and spent tea leaves (ST) on the performance and gut development of broiler chickens. Methods: A total of 240 one-day-old unsexed broiler chicks (ROSS 308) were raised on litter-floored pens and had ad libitum access to water for 42 days. The experiment was conducted on chicks fed with basal diet (CON), commercial mannan-oligosaccharides (MOS), crude oligosaccharide extract from CMM, and crude oligosaccharide extract from ST. The experimental diets were supplemented with 2 and 1 g/kg oligosaccharides during the starter and grower periods, respectively. Results: The body weight gain (BWG) of birds in the MOS group was higher than that of birds in the other groups (p<0.05) in the starter period. However, during the grower period, ST significantly improved the BWG compared to the MOS (p<0.05). MOS, CMM, and ST showed no influence on the carcass and visceral organ weight and the weight and length of intestine (p>0.05). The digestibility of gross energy was greater (p<0.05) in the CMM group than in the CON group during the grower period. Morphological changes were absent in the dietary treatments (p>0.05). Conclusion: The improvements in the growth performance were partly driven by nutrient digestibility of such oligosaccharides having prebiotic properties. This result can indicate that supplementing broiler diets with crude oligosaccharides from CMM and ST had no negative effect on the growth performance and gut development of broilers.

• 대한화학회지
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• 제42권3호
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• pp.297-301
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• 1998

올리고당의 구조중 연결위치(linkage position)는 충돌 탄뎀 질량분석법(FAB CAD MS/MS: Fast Atom Bombardment Collision Activated Dissociation Mass Spectrometry/Mass Spectrometry)을 이용하여 알 수 있다. 연결위치-이성질체 올리고당을 아세틸화시키면 자유올리고당보다 연결위치의 구별이 쉽고 독특한 분절이온 패턴을 얻을 수 있다. 그 이유는 연결위치에 따라 각 올리고당이 충돌에너지(collision energy)를 흡수하여 glycosidic 결합 주위를 회전하는 회전운동의 자유도가 달라지기 때문이다.

• KSBB Journal
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• 제13권3호
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• pp.284-288
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• 1998

Production of inulo-oligosaccharides from inulin was carried out with the maximum yield of 94% using a purified endoinulinase from Aspergillus ficcum. The optimum reaction temperature and pH were 55-60$^{\circ}C$ and pH 5.5-6.0, respectively. The Michaelis constant and maximum reaction velocity of the endoinuinase for inulin were 13.27 g/L and 0.13 g/L$.$min at 55$^{\circ}C$, pH 5.5, respectively. Inulin source had no significant effect on oligosaccharide yield and product composition, although initial production rate differed according to inulin origins. The reaction pH was a critical factor in inulo-oligosaccharide production because considerable free monosaccharides were released, decreasing oligosaccharide yield at low pH conditions. An empirical relationship describing the reaction performance was developed from kinetic data: the time to reach maximum oligosaccharide yield (tw) as a function of initial concentration of inulin (lo) and enzyme (Eo); i.e., log tM = -1.025 log Eo - 0.011 l0 + 2.655.

• 생명과학회지
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• 제9권4호
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• pp.423-429
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• 1999

Carrageenan oligosaccharides prepared from -carrageenan by carrageenase from Pseudomonas alcaligenes. The oligosaccharides showed three spots on TLC and the degree of Polymerization of the C1, C2 and C3 spot were each 9.0$\pm$1.0, 6.0$\pm$1.5 and 2.5$\pm$1.5, respectively. Each hydrolysates and spots-C1, C2, C3-were tested the several functionalities such as antimicrobial activity, anticavity activity and anticoagulant activity. The antimicrobial and anticavity activity of carrageenan hydrolysates and oligosaccharide fractions were very low, but the anticoagulant activity was identified in all samples.

• Bulletin of the Korean Chemical Society
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• 제26권9호
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• pp.1347-1353
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• 2005

Sets of sialic acid-containing trisaccharides having different internal and terminal linkages have been synthesized to develop a sensitive method for analysis of the reducing terminal linkage positions. The trisaccharides, sialyl($\alpha$ 2-3)Gal($\beta$ 1-3)GalNAc and sialyl($\alpha$ 2-3)Gal($\beta$ 1-X)GlcNAc where X=3, 4 and 6, were synthesized and examined using electrospray ionization (ESI)-collision induced dissociation (CID) tandem mass spectrometry (MS/MS). The compounds chosen for this study are related to terminal groups likely to be found on polylactosamine-like glycoproteins and glycolipids which occur on the surface of mammalian cells. The purpose of this study is to develop tandem mass spectrometral methods to determine detailed carbohydrate structures on permethylated or partially methylated oligosaccharides for future applications on biologically active glycoconjugates and to exploit a faster method of synthesizing a series of structural isomeric oligosaccharides to be used for further mass spectrometry and instrumental analysis.

• BMB Reports
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• 제30권4호
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• pp.253-257
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• 1997

Negative ion fast atom bombardment (FAB) mass spectra were found to allow the determination of the linkage positions in a series of underivatized linkage-isomeric oligosaccharides. A previous work (Laine et al., 1988) reported that ion patterns of linkage-isomeric trisaccharides could be distinguished by a positive ion. Negative ion FAB collison-activated dissociation (CAD) mass spectrometry (MS) spectra of trisaccharides exhibited better sensitivity than the positive ion mode and provided specific fragmentation patterns according to the linkage positions. Especially, the fragmentations, m/z 205 in F6 and m/z 221 in G6, not occuring in 1-3 or 1-4 linkage. were an indication of 1-6 linkage, by changing collision energies from + 10 eV to +60 eV. The survival ratios of molecular ions in each collision energy set gave support to previous results in which the order of bond stability was 1-6>1-4>1-3 linkage.