• 한국식품영양과학회지
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• 제28권3호
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• pp.619-624
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• 1999

The aim of this study was to evaluate the effects of oligosaccharides(fructo or isomalto ) contained in sponge cake on blood lipids and intestinal physiology in rats. The experimental diet was mixed with 30% sponge cakes. Normal male Sprague Dawley rats weighing about 530g were randomly assigned to three groups and placed experimental diets and deionized water at free access for 25 days. Rat in control group received a diet mixed with sponge cake containing sucrose only at 21.58g/100g diet and rats in the other two groups received diets mixed with sponge cakes of which 40% sucrose was replaced with each oligosaccharide. The results obtained were as follows: No significant differences in efficiency of food, liver weight and intestinal length were observed among groups. The cecal contents and cecal wall weights were increased more in fructooligosaccharide(FO) group than control. The pH of cecal contents decreased significantly in two oligosaccharide groups. The water contents of fresh feces in FO group were the highest and the those of two oligosaccharide groups were higher than that of control. Dry fecal weight increased significantly in isomaltooligosaccharide(IMO) group. Total serum cholesterol concentration was significantly lower in FO group. Serum triglyceride(TG) was not significantly different among three groups at p<0.05, but serum TG of FO group was lower than those of other groups. In conclusion, 40% replacement of sucrose with oligosaccharides in sponge cake may have beneficial effects on lipid metabolism and intestinal function in rats.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제24권6호
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• pp.501-509
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• 2021

Extensive studies have shown that breast milk is the best source of nutrition for infants, especially during the first six months, because it fulfills almost all of their nutritional needs. Among the many functional building blocks in breast milk, human milk oligosaccharides (HMOs) have been receiving more attention recently. Furthermore, it is the third most common group of compounds in human milk, and studies have demonstrated the health benefits it provides for infants, including improved nutritional status. HMOs were previously known as the 'bifidus factor' due to their 'bifidogenic' or prebiotic effects, which enabled the nourishment of the gastrointestinal microbiota. Healthy gastrointestinal microbiota are intestinal health substrates that increase nutrient absorption and reduce the incidence of diarrhea. In addition, HMOs, directly and indirectly, protect infants against infections and strengthen their immune system, leading to a positive energy balance and promoting normal growth. Non-modifiable factors, such as genetics, and modifiable factors (e.g., maternal health, diet, nutritional status, environment) can influence the HMO profile. This review provides an overview of the current understanding of how HMOs can contribute to the prevention and treatment of nutritional issues during exclusive breastfeeding.

• Animal Bioscience
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• 제35권8호
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• pp.1195-1204
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• 2022

Objective: The objective of this study was to investigate the in vitro fermentation profiles of different soybean oligosaccharides (SBOs) and their effects on skatole production and cecal microbiota of broilers. Methods: Five SBOs with varying main component contents were fermented using an in vitro batch incubation inoculated with broiler cecal microbiota. Gas production was recorded automatically, skatole, indole and short-chain fatty acids (SCFAs) were determined using high-performance liquid chromatography, and microbial changes were analyzed using 16S DNA gene sequencing. Results: The addition of SBOs increased (p<0.05) gas production, suggesting bacterial growth-stimulating activities. In addition, the concentrations of indole were significantly (p<0.05) decreased after SBO supplementation, and SBO III, with higher sucrose and stachyose contents, decreased (p<0.05) the skatole level. Our results also revealed that the fermentation of SBOs by cecal microbiota produced (p<0.05) SCFAs, which were dominated by propionic acid, butyrate acid and lactic acid compared to the control. In addition, SBO III increased (p<0.05) the abundance of Firmicutes and Subdoligranulum and decreased that of Bacteroides. Conclusion: These results suggest that SBOs with higher sucrose and stachyose contents are promising prebiotics in modulating gut microbiota and reducing odor emission in broilers.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제25권6호
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• pp.510-518
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• 2022

Purpose: Recently, great interest has been focused on dietary fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPs) for the treatment of functional abdominal pain (FAP). Several meta-analyses, including those on the adult population, have been published, potentiating its role. However, pediatric studies are scarce. We aimed to evaluate the effect of a low-FODMAP diet on the severity of FAP in children. Methods: This clinical trial included 50 patients aged 3-18 years with irritable bowel syndrome and FAP that were not otherwise specified. The patients were instructed to receive a low FODMAP diet guided by a dietitian. The primary outcome was the percentage of responders after 2 months of dietary intervention compared with baseline. Other outcomes included changes in stool consistency and quality of life (QoL) scores using the KIDSCREEN-10 questionnaire, and weight-for-age z-scores. Results: After the dietary intervention, 74% of patients showed more than 30% lower pain intensity, as examined using the Wong-Baker Faces pain rating scale. Their QoL significantly improved, and patients have gained weight. Conclusion: A low FODMAP diet can improve pain intensity and QoL among children with functional abdominal pain, with no detrimental effects on body weight.

• Applied Biological Chemistry
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• 제36권4호
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• pp.304-309
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• 1993

돼지감자 괴경의 수확시기 및 저장온도에 따른 가용성당의 조성변화를 HPLC 방법으로 조사하였다. 성숙된 괴경의 가용성당은 저온충격을 받은 후 수확한 시료에서부터 급격히 변화하여 봄에 수확한 시료의 경우, 중합도가 높은 GF8이상의 inulin은 총당의 66.4%에서 25.4%로 감소한 반면, GF2부터 GF7까지의 fructo 올리고당은 28.8%에서 61%로, sucrose는 3.4%에서 13.6%로 각각 증가하였다. 이러한 자가효소에 의한 inulin의 fructo 올리고당 및 sucrose로의 전환은 저온 충격을 받지않은 괴경을 $4^{\circ}C$에서 저장할 때에도 나타나서 $25{\sim}34$일 저장으로 거의 비슷한 결과를 보였다. 이와는 달리 fructo 올리고당이 61%에 달하는 3월 수확시료를 각각 $25^{\circ}C{\sim}40^{\circ}C$에서 3일간 저장한 경우에는 fructo 올리고당은 감소하나 sucrose는 증가하며 GF8이상의 inulin도 증가하거나 일정하여 저온저장과는 달리 fructo 올리고당이 감소하고 inulin의 합성이 일어나는 것으로 나타났다. 이상의 결과를 종합해 볼 때 괴경내의 fructo 올리고당 함량을 최대화하기 위해서는 돼지감자괴경을 포장에서 월동시켜 3월 중순에 수확하거나 또는 저온 충격을 받지 않은 것을 저온저장하는 것이 효과적인 방법으로 보인다.

• 한국식품과학회지
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• 제34권1호
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• pp.96-102
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• 2002

대두 제품 중 대두, 두유, 두부, 생대두분말, 분리대두단백(isolated soy protein, ISP), 된장, natto, tempeh의 이소플라본, 수용성 식이섬유, 대두 올리고당, 레시틴, 사포닌의 함량을 분석하였다. 건물 기준으로 생대두분말과 natto, 두유에는 각각 489.1 mg%, 308.3 mg%, 138.1 mg%의 이소플라본이 함유되어 있었다. 이소플라본의 aglycone/glucoside 비율을 natto에 비하여 된장과 tempeh가 더 높았다. 생대두분말은 24.3%의 식이 섬유와 2.87%의 대두 올리고당을 함유하고 있었다. 두유의 경우 식이 섬유의 절대량은 많지 않으나 soluble fiber/total dietary fiber 비가 59%로 가장 높았다. 건물기준으로 두부에는 20.1%의 식이 섬유와 1.13%의 레시틴이 함유되어 상대적으로 많았다. 분리대두단백에는 레시틴과 사포닌이 함유량은 각각 0.63%와 0.65%였다. 비록 대두의 생물학적 활성물질함량은 레시틴을 제외하고 대두 가공품 중 가장 높았으나 이를 일회 분량으로 환산한 경우 두유 내 생물학적 활성물질의 양이 대두와 매우 유사하였다. 즉 두유 일회 분량(200 mL)에는 이소플라본 31.5 mg(raw soybean 15.9 mg), 식이섬유 2.59 g(raw soybean 4.59 g), 올리고당 0.57 g(raw soybean 0.50 g), 레시틴 0.10 g(raw soybean 0.10 g), 사포닌 0.11 g(raw soybean 0.10 g)이 함유되어 있어, 두유는 일상적으로 섭취하기 편하고 대두 생리활성 물질의 좋은 공급원이 될 수 있을 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.187-194
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• 2022

Two α-ʟ-arabinofuranosidases (BfdABF1 and BfdABF3) and a β-ᴅ-xylosidase (BfdXYL2) genes were cloned from Bifidobacterium dentium ATCC 27679, and functionally expressed in E. coli BL21(DE3). BfdABF1 showed the highest activity in 50 mM sodium acetate buffer at pH 5.0 and 25℃. This exo-enzyme could hydrolyze p-nitrophenyl arabinofuranoside, arabino-oligosaccharides (AOS), arabinoxylo-oligosaccharides (AXOS) such as 3²-α-ʟ-arabinofuranosyl-xylobiose (A³X), and 2³-α-ʟ-arabinofuranosyl-xylotriose (A²XX), whereas hardly hydrolyzed polymeric substrates such as debranched arabinan and arabinoxylans. BfdABF1 is a typical exo-ABF with the higher specific activity on the oligomeric substrates than the polymers. It prefers to α-(1,2)-ʟ-arabinofuranosidic linkages compared to α-(1,3)-linkages. Especially, BfdABF1 could slowly hydrolyze 2³,3³-di-α-ʟ-arabinofuranosyl-xylotriose (A²⁺³XX). Meanwhile, BfdABF3 showed the highest activity in sodium acetate at pH 6.0 and 50℃, and it has the exclusively high activities on AXOS such as A³X and A²XX. BfdABF3 mainly catalyzes the removal of ʟ-arabinose side chains from various AXOS. BfdXYL2 exhibited the highest activity in sodium citrate at pH 5.0 and 55℃, and it specifically hydrolyzed p-nitrophenyl xylopyranoside and xylo-oligosaccharides (XOS). Also, BfdXYL2 could slowly hydrolyze AOS and AXOS such as A³X. Based on the detailed hydrolytic modes of action of three exo-hydrolases (BfdABF1, BfdABF3, and BfdXYL2) from Bf. dentium, their probable roles in the hemiceullose-utilization system of Bf. dentium are proposed in the present study. These intracellular exo-hydrolases can synergistically produce ʟ-arabinose and ᴅ-xylose from various AOS, XOS, and AXOS.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.141-141
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• 2005

• 대한지역사회영양학회:학술대회논문집
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• 대한지역사회영양학회 1998년도 추계학술대회 및 제3차 정기총회
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• pp.106-106
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• 1998

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 1998년도 수산관련공동학술대회발표요지집
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• pp.258.2-259
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• 1998