• 한국동물생명공학회지
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• 제36권4호
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• pp.292-298
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• 2021

Olfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was reported and the possible roles of OR1I1 in the testicular cells were suggested. The objectives of this study were 1) to explore the expression of OR7D4 and OR1I1 in stallion testes, and 2) to define the specific localization of OR7D4 and OR1I1 in the testicular tissues. Stallion testicular tissue samples were used for this study. Western blot was performed to confirm the cross-reactivity of OR7D4 and OR1I1 antibody with stallion testicular tissue samples. OR7D4 and OR1I1 gene expressions were investigated using reverse transcription-polymerase chain reaction (RT-PCR) in stallion testes. Immunofluorescence was performed to investigate the expression of OR7D4 and OR1I1 in stallion testicular tissues. The protein bands for OR7D4 and OR1I1 from the testes were observed at approximately 38 kDa and 43 kDa, respectively. The mRNA of OR7D4 and OR1I1 were detected in stallion testes. Immunolabeling of OR7D4 and OR1I1 in the cytoplasm of both spermatogonia and Leydig cells was observed. In conclusion, androstenone and another odorant chemical, which is recognized by OR1I1, may play an important role in stallion testes.

• Environmental Analysis Health and Toxicology
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• 제25권2호
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• pp.131-143
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• 2010

We performed the tests of acute and subchronic inhalation toxicity of methyl formate, which has limited toxicological data in spite of its widespread use and enhanced hazard consequent on its high volatility. The median lethal concentration ($LC_{50}$) was evaluated to be above 5,000ppm(12.27 mg/L). In the test with subchronic inhalation, there are no deaths, but with reduction of body weight, food intake, organ weight by exposure to 400 (0.98 mg/L) and 1,600 (3.92 mg/L) ppm, dose-dependently. There were statistical differences in some hematological and blood biochemical parameters as compared to control (e.g. neutrophile and lymphocyte in the 1,600 ppm group, calcium and A/G in 1,600 ppm group). Methyl formate under the exposure of 1,600 ppm showed the respiratory findings with nasal, it was confirmed that the chemical has respiratory hazard with 1,600 ppm inhalation exposure, induces nasal epithelial atrophy, olfactory cell degeneration/regeneration and the contraction of olfactory cells, etc. According to the notification with Ministry of Labor (No. 2009-68) for classification, labeling and MSDS of chemicals, it is suggested for methyl formate to be classified as category 4 in acute (10.0$4\leq20.0$ mg/L), category 2 (0.2$\leq$1.0 mg/L/6h, 90 days) in specific target organ-repeated exposure.

• 한국동물생명공학회지
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• 제36권4호
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• pp.270-284
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• 2021

The vomeronasal organ (VNO) is critical for reproduction and social behavior in ruminants, including cattle. The present study examined the structure of the VNO and its epithelial cells in neonatal and adult Korean native cattle (Hanwoo), Bos taurus coreanae, using immunohistochemistry and lectin histochemistry. Histologically, the VNO comprised two types of epithelia: medial vomeronasal sensory (VSE) and lateral vomeronasal non-sensory epithelia (VNSE). Numerous blood vessels and nerve bundles were observed within the vomeronasal cartilage encased lamina propria. Immunohistochemistry revealed high expression level of protein gene product9.5 and moderate expression level of olfactory marker protein in the neuroreceptor cells of the VSE and occasionally in some ciliated cells of the VNSE in both neonates and adults. The properties of the glycoconjugates in the VNO were investigated using 21 lectins, most of which were expressed at varied intensities in the VSE and VNSE, as well as in the lamina propria. Several lectins exhibited variations in their intensities and localization between neonatal and adult VNOs. This study is the first descriptive lectin histochemical examination of the VNO of Korean native cattle with a focus on lectin histochemistry, confirming that the VNO of Korean native cattle is differentiated during postnatal development.

• BMB Reports
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• 제42권10호
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• pp.631-635
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• 2009

The heat shock transcription factor (HSF) family consists of at least three members in mammals and regulates expression of heat shock proteins in response to heat shock and proteotoxic stresses. Especially, HSF1 is indispensable for this response. Members of this family are also involved in development of some tissues such as the brain and reproductive organs. However, we did not know the molecular mechanisms that regulate developmental processes. Involvement of HSFs in the sensory development was implicated by the finding that human hereditary cataract is associated with mutations of the HSF4 gene. Analysis of gene-disrupted mice showed that HSF4 and HSF1 are required for the lens and the olfactory epithelium, respectively. Furthermore, a common molecular mechanism that regulates developmental processes was revealed by analyzing roles of HSFs in the two developmentally-related organs.

• 한국동물학회지
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• 제10권1호
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• pp.10-16
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• 1967

본 연구는 백서후각기의 발생과정을 세포조직학적으로 규명보고하는 바이며 사용된 stage는 stage 20부터 32까지는 Christe(1964)의것을 인용하였으며 그 외에 명시된 stage 33,34, 35 는 저자가 편의상 구분하였다. 재료는 백서 5 필을 온실서 충분한 영양으로 사육후 임신시켜 요구되는 배를 stage 별로 얻어 Bouin's solution 에 고정시켜 paraffin법을 따랐으며 5-7$\mu$의 두께로 절편을 만들어 Delafield's hematoxylin, eosin, thionin 에 염색하였다. Stage 별 후각기의 발생은 state 20 에 후순, state 22에 후와 및 조비기(Jacobson's organ), stage23A에 구비강막(oro-nasal membrane), state 23 B 에 수신경섬유 와 원시내비공, stage 24 에 조비기신경섬유, stage 27에는 골갑개상피에 후세포와 호흡상피상에 섬유 또한 악골갑개상피에서 seromucinous 선들이 최초로 나타났다. 본 실험결과 백서후각기의 형성과정은 인체와 생쥐( mouse) 에서와 유사하나 조비기의 성장, 비갑개의수, 및 후역의 범위에 있어서는 극히 차가 심한 것같다.

• 한국실내디자인학회논문집
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• 제22권1호
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• pp.291-298
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• 2013

A science museum responds independently based on the exhibits and exhibition environments as the visitors are different in purposes, interests and demands. Therefore a science museum should be designed keeping it in mind that there are various ways for visitors to perceive and use the exhibition spaces and exhibits. The purpose of this study is to compare and analyze the characteristics of sensory receptors for the exhibits in National Museum of Nature and Science's Global Gallery, Tokyo, in terms of information transmission and to identify the nature of exhibit medium that can affect the perception and recognition of the exhibits by visitors. Through these 9 sensory receptors, human recognizes first with visual, auditory and olfactory senses and reacts using vestibular organ, proprioceptor (stretch), tangoreceptor, themoreceptor, taste and olfactory senses. Human uses these information processing to recolonize the external environment. This process is similar to the visitor's information transmission process for the exhibition medium. By dividing the analysis results per exhibition theme and developing the information transmission processing types per sensory receptor, we could understand that the distribution conditions are closely connected with the composition of the exhibition scenario in the exhibtion area. Especially, the understanding of how the information transmission is made through sensory receptors could can be the criteria that determines on the factors that can identify the exhibition purposes of a science museum which are eduction and understanding.

• 한국수산과학회지
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• 제31권3호
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• pp.309-316
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• 1998

참복과 (Tetraodontidae) 어류의 외부형태 형질 가운데 후각기관, 옆줄, 피부극. 체형 및 체색의 특징을 조사하기 위하여. 1989년 9월부터 1994년 7월까지 우리나라 20개 지점에서 10속 27종의 표본을 채집하여 관찰한 결과는 다음과 같다. 1. 참복과 어류 가운데 참복속(Takifugu), 밀복속(Lagocephalus), 은띠복속(Plurancanthus). 불룩복속(Spheoides) 및 대치복속 (Feroxodon) 어류의 콧구멍은 2개이며. 수지복속 (Ephippion)과 청복속 (Canthigaster) 어류는 1개이고, 반면에 별복속 (Boesemanichthys), 꺼끌복속(Arothron) 및 첼로복속 (Chelonodon) 어류는 없다. 2. 밀복속, 불룩복속, 별복속, 꺼끌복속 및 대치복속어류의 옆줄은 몸의 옆쪽에 단지 1열로 잘 발달되어 있으며, 참복속과 은띠복속 어류는 2열이고, 첼로복속과 수지복속 어류는 꼬리자루 부분에서 2열로 융합되어 있다. 3. 청복속 어류는 옆줄 및 후각기관의 형태가 참복과의 다른 속 어류와 많은 차이를 보여 구별되었다 4. 수지복(Ephippion guttifer)의 피부극은 비늘이 완전하게 변형되어 등쪽과 옆쪽의 것은 딱딱한 나무껍질 모양을 하며, 꼬리쪽의 것은 긴 막대기 모양으로 특이하였다. 5. 피부극을 가지고 있는 종은 22종으로 밀복속 어류와 참복속의 까칠복 (Takifugu stictonotus) 및 꺼끌복속(Arothron)의 꺼끌복 (A. Stellatus)의 피부극은 등쪽의 것보다 배쪽의 것이 크고, 첼로복 (Chelonodon patoca)과 청복 (Canthigaster tivulata)은 등쪽의 피부극의 뿌리가 3개이었다. 6. 밀복속 어류의 대부분은 피부극을 등쪽과 배쪽에 가지고 있으나, 민밀복 (L. inermis)은 몸 전체에 피부극이 없는 점에서 밀복 (L. lunaris), 은밀복 (L. wheeleri), 흑밀복 (L.gloveri) 및 은민밀복 (L. laevigatus)과 구별된다.

• 한국초등과학교육학회지:초등과학교육
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• 제22권1호
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• pp.121-129
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• 2003

The enhancement of inquiry skills has been emphasized as a important objective of science education for a long time. Of these, the observation is not only a simple and basic skill, but also a very important skill, in aspect of gathering informations about the nature of all things around us, through interaction between the sense organs of body and objectives. The purpose of this study is to investigate the results of observations about the grasshopper(Oxya chinesis), made by the elementary student from the 3rd to the 6th grade, and to make use of them as the basic materials for the observative teaming and the evaluation of the observation ability. Through this study, the collected items of observation are as follows For grasshopper, a total of observation items is 95, 70 using the sight sense, 13 using the tactile sense,7 using the olfactory sense. 3 using the palate sense and 2 using the auditory sense. In this study, the findings of elementary student's observation are as follows. 1. On the whole, most of students have observed mainly by the sight and the tactile sense, when observing the grasshopper. 2. It is showed a tendency that the observation ability of student is increased with the higher grade in elementary school. 3. As the grade ascends. the observations with operating are increased, also the quantitative expression and interpretation about them are increased. 4. In the case of same grade, there is no significant difference between students' gender, though girls' ability of the observation showed somewhat higher than boys' 5. Occasionally, the interpretations on the observative facts made by student, are inaccurate. Basis on the above results, we suggested some directions for the improvement of the observative learning program in science classroom of elementary school. First, the teacher have to serve as a guide and encouragement in the observative learning class, to be accomplished the various observation, which all the sensory organ can be used by student than the sight sense. Second, to get elevated the ability of observation, it is necessary that some experimental tools(magnifying lens, stereoscope, auxiliary implements etc.) are utilized. Third, the teacher have to make often endeavors showing an example of operation, to be activated the atmosphere of operative observation.

• Toxicological Research
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• 제33권4호
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• pp.333-342
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• 2017

Ethyl formate, a volatile solvent, has insecticidal and fungicidal properties and is suggested as a potential fumigant for stored crop and fruit. Its primary contact route is through the respiratory tract; however, reliable repeated toxicological studies focusing on the inhalation route have not been published to date. Therefore, the present study was conducted to investigate the safety of a 90-day repeated inhalation exposure in rats. Forty male and 40 female rats were exposed to ethyl formate vapor via inhalation at concentrations of 0, 66, 330, and 1,320 ppm for 6 hr/day, 5 days a week for 13 weeks. Clinical signs, body weights, food consumption, urinalysis, hematologic parameters, serum chemistry measurements, organ weights, necropsy, and histopathological findings were compared between the control and ethyl formate-exposed groups. Locomotor activity decreased during exposure and recovered afterward in male and female rats exposed to 1,320 ppm ethyl formate. Body weight and food consumption continuously decreased in both sexes exposed to 1,320 ppm ethyl formate from week 1 or 3 compared with the control values. The increases in adrenal weight and decreases in thymus weight were noted in both sexes exposed to ethyl formate at 1,320 ppm. Degeneration, squamous metaplasia of olfactory epithelium in the nasopharyngeal tissue, or both were noted in the male and female rats at 1,320 ppm and female rats at 330 ppm ethyl formate. Taken together, our results indicate that ethyl formate-induced changes were not observed in male and female rats at 330 and 66 ppm, respectively. This indicates that exposure to ethyl formate at concentrations below 66 ppm for 90 days is relatively safe in rats. This is the first report of a full-scale repeated inhalation toxicity assessment in rats and could contribute to controlling occupational environmental hazards related to ethyl formate.

• 농업과학연구
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• 제27권1호
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• pp.13-19
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• 2000

SEM을 이용하여 아메리카잎굴파리 (Liriomyza trifoljj) 의 암 수컷 성충의 외부형태적 특정을 살펴보았다. 더듬이에 있어 감각모의 3가지 형태는 더듬이가는마디(funicle)에서 관찰되었으며, 모상감각기(trichoid sensillum)는 말단 부위가 뽀족한 특정적인 형태를 하고 있고 감각기들 중에서 가장 많이 분포하고 있다. 원추상 감각기(basiconic sensillum)는 길이가 $13{\mu}m$, 지름이 $1.4{\mu}m$에서 $1.2{\mu}m$인 원형곡선형이다. 홈형 감각기 (grooved sensillum)는 간상 (peg) 끝에서 나온 손 모양을 하고 있으며, 이 모든 감각기들은 후각과 연관되어져 있는 것으로 추정된다. 구기는 흡즙형으로 작고 펼쳐진 윗입술(labrum) 과 합쳐져서 하나의 연결된 주둥이(rostrum)을 형성하고 있다. 가느다란 한 쌍의 작은턱수염 (maxillary palp)은 긴 관 모양으로 각각의 세로로 이어져 있다. 발마디는 5마디로 이루어져 있으며 그 한가운데에는 패드모양의 흡착반(pulvillus)과 가시모양의 발톱사이돌기(empodium)가 있다. 아메리카잎굴파리는 흡착반과 발톱사이돌기에서 미끄러운 표변에 부착할 수 있는 물질을 분비한다. 수컷의 생식기는 매우 복잡하며 삽입구(aedeagus)는 생식기관으로서 가장 일반적인 모양이다. 반면에 암컷의 생식기는 상대적으로 간단한데 이 구조는 외부로 돌출된 관모양이었다. 또한 이러한 결과를 이미 발표된 다른 여러 종의 파리와 비교하였다.