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Acute and Subchronic Inhalation Toxicity Evaluation of Methyl Formate in Rats  

Kim, Hyeon-Yeong (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute)
Lee, Sung-Bae (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute)
Han, Jeong-Hee (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute)
Kang, Min-Gu (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute)
Yang, Jeong-Sun (Chemical Safety & Health Research Center, Occupational Safety & Health Research Institute)
Publication Information
Environmental Analysis Health and Toxicology / v.25, no.2, 2010 , pp. 131-143 More about this Journal
Abstract
We performed the tests of acute and subchronic inhalation toxicity of methyl formate, which has limited toxicological data in spite of its widespread use and enhanced hazard consequent on its high volatility. The median lethal concentration ($LC_{50}$) was evaluated to be above 5,000ppm(12.27 mg/L). In the test with subchronic inhalation, there are no deaths, but with reduction of body weight, food intake, organ weight by exposure to 400 (0.98 mg/L) and 1,600 (3.92 mg/L) ppm, dose-dependently. There were statistical differences in some hematological and blood biochemical parameters as compared to control (e.g. neutrophile and lymphocyte in the 1,600 ppm group, calcium and A/G in 1,600 ppm group). Methyl formate under the exposure of 1,600 ppm showed the respiratory findings with nasal, it was confirmed that the chemical has respiratory hazard with 1,600 ppm inhalation exposure, induces nasal epithelial atrophy, olfactory cell degeneration/regeneration and the contraction of olfactory cells, etc. According to the notification with Ministry of Labor (No. 2009-68) for classification, labeling and MSDS of chemicals, it is suggested for methyl formate to be classified as category 4 in acute (10.0 mg/L), category 2 (0.21.0 mg/L/6h, 90 days) in specific target organ-repeated exposure.
Keywords
Methyl formate; Inhalation toxicity; $LC_{50}$;
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1 Peng HM, Raner GM, Vaz AD and Coon MJ. Oxidative cleavage of esters and amides to carbonyl products by cytochrome P450, Arch Biochem Biophys 1995; 318(2): 333-339.   DOI   ScienceOn
2 Thompson M. NTP technical report on toxicity studies of formic acid: Administered by inhalation to F344/N rats and B6C3F1 mice, National Toxicology Program 1992.
3 Boorman GA, Eustis SL, Elwell MR, Montgomery CA, Jr. and Mackenzie WF. Pathology of the Fischer rat, Academic Press 1990.
4 Kittel B, Ruehl-Fehlert C, Morawietz G, Klapwijk J, Elwell MR, Lenz B, O'Sullivan MG, Roth DR, Wadsworth PF. RITA Group and NACAD Group. Revised guides for organ sampling and trimming in rats and mice-Part 2, Exp Toxicol Pathol 2004; 55(6): 413-431.   DOI   ScienceOn
5 OEHHA. Health effects of exposure to methyl formate, a chemical petitioned for exemption from VOC rules. Memorandum to the Air Resources Board from the Office of Environmental Health Hazard Assessment, Available on-line at 2008: http://www.arb.ca.gov/research/reactivity/methyl-formate-letter.pdf.
6 Nihlen A and Droz PO. Toxicokinetic modelling of methyl formate exposure and implications for biological monitoring, Int Arch Occup Environ Health 2000; 73(7): 479-487.   DOI   ScienceOn
7 OECD. OECD Guideline for Testing of Chemicals: 403 Acute Inhalation Toxicity, 2008.
8 OECD. OECD Guideline for Testing of Chemicals: 413 Subchronic Inhalation Toxicity: 90-day study, 2004.
9 British Toxicology Society Working Party on Toxicity. A new approach to the classification of substances and preparations on the basis of their acute toxicity, Human Toxicol 1984; 3(2): 85-92.   DOI
10 CAS(Chemical Abstracts Service; Adivision of the American Chemical Society). 2008.8. Avalilable from: http://www.cas.org/index.html.
11 Giknis MLA and Clifford CB. Clinical Laboratory Parameter for Crl :CD(SD) Rats, Charles River Laboratories 2006.
12 Jones TC, Mohr U and Hunt RD. Respiratory system, Springer 1996.
13 박희찬. 유해.위험성 평가를 위한 우선 평가대상 물질 목록작성 연구, 산업안전보건연구원 2007.
14 한국산업안전보건공단. 물질안전보건자료(MSDS), 한국산업안전보건공단 2009.
15 국립환경과학원. 화학물질유해성시험연구기관의 지정 등에 관한 규정 2004.
16 환경부. 제3차 화학물질 유통량조사 최종보고서 2007a.
17 後藤稠. 産業中毒便覽, 醫療藥出版(株) 1994; 893-894.
18 강부현, 손화영, 하창수, 이현숙, 송시환. Sprague-Dawley 랫드의 혈액학∙혈액생화학치의 자료분석, 한국실험동물학회지 1995; 11: 141-145.
19 강정부, 권오덕, 김덕환, 김 두, 나기정. 수의임상병리, 기전연구사 2000.
20 강진석, 남기택, 박인승, 안병우, 양기화, 윤창용, 이국경, 장동덕, 제정환, 홍충만. 독성병리학 도감, 청구문화사 2001; 80-81.
21 김현영, 이성배, 강민구, 송시환. 메틸사이클로핵산의 흡입 독성과 유해성 평가, 한국환경독성학회 2006; 21(2): 173-184.   과학기술학회마을
22 노동부. 노동부고시 제2008-26 화학물질 및 물리적인자의 노출기준 2009; 26-27.
23 노동부. 노동부고시 제2009-68호 화학물질의 분류.표시 및 물질안전보건자료에 관한 기준 2009.