• 대한예방한의학회지
• /
• 제9권1호
• /
• pp.49-63
• /
• 2005

Objectives : Ojayeonjonghwan(五子衍宗丸) is composed of Polygonum multiflorum THUNB, and some medical herbs that are known as formula of senescence delay effects. The purpose of this study is to investigate th effect of Ojayeonjonghwan on antioxidant enzyme activities, such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione preoxidase (GSH-px) in rat erythrocytes and blood plasma. Methods : Sprague-Dawley rats were divided into 3 groups, Normal group (supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Ojayeonjonghwan administered group (D-galactose 50mg/kg and Ojayeonjonghwan extracts 245.0mg/200g 1time/day for 6 weeks, OJY Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : TBARS in plasma concentration of OJY group was significantly lower than that of control group. Red blood cell(RBC) SOD activity of OJY group was significantly higher than that of control group(F=16.057, p=0.0001, ANOVA test), RBC GSH-px activity of OJY group was increased(F=4.271, p=0.034, ANOVA test). RBC catalase activities of all experimental group were not significantly different. Total lipid and triglyceride concentration in plasma of all experimental groups were not significantly different. Total cholesterol concentrations in plasma of OJY group were significantly lower than those of control group(F=4.387, p=0.032, ANOVA test). Conclusions : According to the above results, it is considered that Ojayeonjonghwan is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

• 대한한의학방제학회지
• /
• 제17권1호
• /
• pp.113-120
• /
• 2009

Objectives : This study focuses on the effect of the extract which We manufactured by adding Aliium sativum to Ojayeonjonghwan on the amount of semen, the number and the activity of sperm. Methods : We observed any changes in the amount of semen, the number and the activity of sperm after orally administering the test drug for thirty days and taking semen samples from a selected group of men - healthy men in their 20s, as well as men in their 40s who were feeling a decrease in energy. Results : This experiment showed the result that the amount of semen increased by adding Aliium sativum to Ojayeonjonghwan in the case of men in their 20s, but did not show a statistically significant result. It showed the result that the amount of semen increased by a statistically significant level as well as the activity of sperm. Consequently, the result indicated that orally administering the extract added Aliium sativum to Ojayeonjonghwan increased the amount of semen, the number and the activity of sperm for men in their 20s. Men in their 40s who are feeling a decline in energy generally decreased the amount of semen, the number and the ratio of activity of sperm. However the amount of semen remarkably increased and the number and the activity of sperm increased but the result was not statistically significant after orally administering the test drug for thirty days. Conclution : Eventually, the result after orally administering the test drug to the group of men in their 20s and 40s for thirty days indicated that both groups increased the amount of semen, number of sperm and activity of sperm to a statistically significant degree.

• 대한한의학방제학회지
• /
• 제29권1호
• /
• pp.1-8
• /
• 2021

Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Ojayeonjonghwan (OYH) in GC-1 germ cell lines. Methods : DPPH radical scavenging activity and cell viability assays in GC-1 germ cell lines were performed. In addition, the protective effects of OYH against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines were examined by measuring cell viability after H2O2 treatmet. The formation of ROS and the antioxidant enzymes activity such as SOD and catalase were measured in the same condition. Results : OYH scavenged DPPH radical dose-dependent manner and the IC50 was 63.79 ㎍/ml. OYH showed no cytotoxicity at concentration of 1, 10, 100 ㎍/ml. The hydrogen peroxide-induced cytotoxicity of GC-1 germ cell lines was protected to 53.66% by OYH at concentration of 10 ㎍/ml. OYH effectively inhibited ROS production in GC-1 germ cell lines. Mn SOD and catalase protein expression were significantly increased in GC-1 germ cell lines, but Cu/Zn SOD protein expression was not significantly changed. Conclusions : In conclusion, OYH has antioxidant activities against hydrogen peroxide-induced oxidative stress in GC-1 germ cell lines.