• Development and Reproduction
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• v.20 no.3
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• pp.255-266
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• 2016

The optimum concentrations of clove oil as an anesthetic for olive flounder (Paralichthys olivaceus) and the stress response of the fish to clove oil anesthesia were determined over a range of water temperatures, and investigated in a simulated transport experiment using analysis of various water and physiological parameters. While the time for induction of anesthesia decreased significantly as both the concentration of clove oil and water temperature increased, the recovery time increased significantly (P<0.05). The plasma cortisol concentration in fish at each temperature increased significantly up to 12 h following exposure (P<0.05), then decreased to 48 h (P<0.05). The DO dissolved oxygen concentrations, pH values, and the fish respiratory frequencies decreased over 6 h following exposure to clove oil in all experimental groups (P<0.05), whereas the $NH_4{^+}$ and $CO_2$ concentrations in all experimental groups increased up to 6 h (P<0.05). The pH values and DO concentrations increased with increasing clove oil concentration (P<0.05) in the 6 h following exposure, and the $CO_2$ and $NH_4{^+}$ concentrations and the respiratory frequencies decreased with increasing clove oil concentration (P<0.05). The results of this experiment suggest that clove oil reduced the metabolic activity of olive flounder, thus reducing $NH_4{^+}$ excretion and $O_2$ consumption. In conclusion, clove oil appears to be a cost-effective and efficient anesthetic that is safe for use and non-toxic to the fish and users. Its use provides the potential for improved transportation of olive flounder.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.532-539
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• 2004

For efficient lincomycin production from Streptomyces lincolnensis L1245, various vegetable oils, natural nitrogen sources, and surfactants were investigated at the pilot-scale level in the flask. Olive oil as the sole carbon source was the most suitable one for producing lincomycin. When 20 g/lof olive oil was used, the lincomycin concentration and lipase activity reached 1.01 g/land 182 U/ml, respectively, after 5 days of culture. Among the various unsaturated fatty acids, when linolenic acid was used, the cell growth and lincomycin production were markedly decreased. On the other hand, when 0.2 g/l of oleic acid was added to the culture broth, the maximum lincomycin concentration was 1.0 g/l, which was about 1.7-fold higher than that obtained without the addition of oleic acid. Among the various natural nitrogen sources, pharmamedia or soybean meal was the most suitable nitrogen source. In particular, in the case of a mixture of 10 g/l of pharmamedia and soybean meal, 1.5 g/l of lincomycin concentration and 220 U/ml of lipase activity were obtained. When Span 180 was used as the surfactant, lincomycin production, lipase activity, and oil consumption increased. The correlation between the consumption rates of oil and lincomycin production in a culture using olive oil as the sole carbon source was also investigated. The lincomycin production depended on the consumption rate of olive oil. Using these results, fed-batch cultures for comparing the use of olive oil and starch as a conventional carbon source were carried out in a 5-1 fermentor. When olive oil was used as the sole carbon source, 34 g/l of olive oil was consumed after 7 days of culture. The maximum lincomycin concentration was 3.0 g/l, which was about 2.0-fold higher than that of starch medium after 7 days of culture. The product yield was 0.09 gig of consumed carbon source, which was about 3.0-fold higher than that of starch medium after 7 days of culture.

• Journal of Soil and Groundwater Environment
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• v.21 no.1
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• pp.72-79
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• 2016

Bioremediation is one of the most effective ways to remediate TPH-contaminated sites. However, under actual field conditions that are not at the optimum temperature, degradation of microorganisms is generally reduced, which is why the efficiency of biodegradation is known to be significantly affected by the soil temperature. Therefore, in this study, the labscale experiment was conducted using indigenous crude oil degrading microorganisms isolated from crude oil contaminated site to evaluate the remediation efficiency. Crude oil degrading microorganisms were isolated from crude oil contaminated soil and temperature, which is a significant factor affecting the remediation efficiency of land farming, was adjusted to evaluate the microbial crude oil degrading ability, degradation time, and remediation efficiency. In order to assess the field applicability, the remediation efficiency was evaluated using crude oil contaminated soil (average TPH concentration of 10,000 mg/kg or more) from the OO premises. Followed by the application of microorganisms at 30℃, the bioremediation process reduced its initial TPH concentration of 10,812 mg/kg down to 1,890 mg/kg in 56 days, which was about an 83% remediation efficiency. By analyzing the correlation among the total number of cells, the number of effective cells, and TPH concentration, it was found that the number of effective microorganisms drastically increased during the period from 10 to 20 days while there was a sharp decrease in TPH concentration. Therefore, we confirmed the applicability of land farming with isolated microorganisms consortium to crude oil contaminated site, which is also expected to be applicable to bioremediation of other recalcitrant materials.

• Journal of The Korean Society of Integrative Medicine
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• v.6 no.3
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• pp.131-139
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• 2018

Purpose : The study objective was to assess the antibacterial activity of essential oil of basil against S. mutans and P. gingivalis and to explore its potential to prevent dental caries and peridontal disease. Method : Essential oil of basil, extracted using steam distillation, was diluted with triple distilled water and Tween 20 to generate samples at various concentration, that is 30%, 50%, and 70% (v/v). Strains of S. mutans and P. gingivalis were incubated in the medium under anaerobic condition. Broth microdilution susceptibility testing and plate incubation diffusion were utilized to determine the minimum inhibitory concentration (MIC) and to measure antibacterial activity, respectively. Result : An upsurge in antibacterial activity was seen to correlate with and increase in the concentration used for both bacterial strains, but was more significant with S. mutans. A statistically significant growth inhibition effect and reduction in the number of colonies was also observed with both strains dependent on the concentration used following 24 hours of incubation. Conclusion : Thus, the current study finding was that essential oil of basil was effective against dental caries and periodontal disease and could be used in dentifrice to help prevent oral disease.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.1
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• pp.60-66
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• 1995

The oxidation of purified pinenut oil containing various concentration of tocopherols and β-carotene were studied. α-tocopherol revealed an antioxidant activity at the concentration of lower than 0.05%, however, it showed a prooxidant activity when the concentration was higher than 0.05%. The antioxidant activity of γ-tocopherol was not affected by the concentraitons in the range of 0.01∼0.10% in pinenut oil. γ -Tocopherol resulted in higher antioxidant activity than that of α-tocopherol. β -carotene seemed to be a prooxidant when 0.01% of β-carotene was added. The fatty acids composition of purified pinenut oil have been changed during autoxidation. The concentration of linoleic acid decreased readly while oleic acid seemed to increase. And the concentration of saturated fatty acid has'nt been changed much during autoxidation.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.12 no.9
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• pp.791-801
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• 2000

An experimental study was conducted to analyze the effects of oil on refrigerant flow through adiabatic capillary tubes, and to develop a model for mass flow rates of refrigerant/oil mixture at various capillary tubes and flow conditions. Mass flow rates and the profiles of the pressures and temperatures along the capillary tubes was obtained with the oil concentration of R-22/SUNISO 4GS oil mixture at various test conditions. The flow trends as a function of geometry and flow conditions for pure refrigerant and refrigerant/oil mixture were similar in adiabatic capillary tubes. Mass flow rate of the refrigerant/oil mixture was less than that of pure refrigerant at the same test conditions.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.3
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• pp.87-99
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• 1999

Manuka oil named New Zealand’s tea tree oil is oil-soluble and comes firom nature. Manuka oil and its extract $\alpha$-pinene, Oxy’less clear, R-limonene which is one of the component of Citron extracted from Grapefruit seed and Citrex were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion method was used to measure the antimicrobial activity. Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus was similar when the concentration of Manuka oil and $\alpha$-pinene are 10u/paper disk. However, antimi-crobial activity of Manuka oil fDr Escherichia coli, Staphylococcus aurem was better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene was low. Antimicrobial activity of Oxy’less clear is better than that of propyl para hydroxybenzoate(PPHB), R-limonene at all the concentration and is similar to that of $\alpha$-pinene. Antimicrobial activity.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1999.06a
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• pp.189-194
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• 1999

The antiwear performance of several organic phosphates ,such as tricrecylphosphate(TCP), tributylphosphate(TBP), diphenylhydrogenphosphate(DPHP) ,dissolved in polyol ester based oils is studied. These organic phosphates are well known for antiwear additive for lubricating oil that produce reacted surface protective film. These antiwear additives can drastically reduce wear with their concentration increasing, because the amount of additive adsorbed on metal sur(ace increases. But in the higher concentration region, the wear is increased by excessive and corrosive reaction of the metal surface with these additives. That is to say, there is an optimum concentration for minimum wear. The optimum concentration was different with the kinds of base oils and additives. Different polyolesters showed different optimum concentrations of the additive. The order of optimum concentration among the polyolesters was different with different phosphates. The order of the optimum concentration is shown that the effect of the concentration of additives on the antiwear performance. It can be explained by the interaction between additives and base oils using the solubility parameter.

• Journal of the Korean Ceramic Society
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• v.37 no.5
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• pp.403-409
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• 2000

The permeation characteristics and reclamation efficiency of waste lubricating oil were studied as a function of the types of ceramic composite membranes and the membrane separation process variables. The oil permeability of the TiO2 composite membrane(pore size 0.015 $\mu\textrm{m}$) was directly proportional to the crossflow velocity(0.22∼0.9 m/s) and temperature(150$^{\circ}C$∼200$^{\circ}C$). In the batch concentration process, as the concentration factor increased, both the permeability and the ash content of the permeate decreased. The average ash contents of the total permeate through the A6 alumina membrane(average pore size 0.8$\mu\textrm{m}$), Z1/A6 and Z1/A4(pore size 0.23$\mu\textrm{m}$)/A7(pore size 6$\mu\textrm{m}$) zirconia composite membrances(average pore size 0.07$\mu\textrm{m}$) were about 0.063 wt%, 0.045wt% and 0.08wt% in the region of 1∼2 concentration factor, respectively. The ash content of the mixed permeate through the A6 alumina and zirconia composite membrane was about 0.06 wt% and it can be also reduced to 0.06 wt% in the Z1/A6 membrane and below 0.003 wt% in the TiO2/Z1/A6 membrane. It was concluded that the treated oil obtained from the multi-step membrane separation process could be used as reclaimed lubricating oil as well as reclained fuel oil.

• Food Science and Biotechnology
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• v.16 no.6
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• pp.999-1005
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• 2007

An oil-in-water (O/W) emulsion [20 wt% com oil, 0.5-6.0 wt% fish gelatin (FG), pH 3.0] was produced by high pressure homogenization, and the influence of pH, protein concentration, and homogenization condition on the formation of FG-stabilized emulsions was assessed by measuring particle size distribution, electrical charge, creaming stability, microstructure, and free FG concentration in the emulsions. Optical microscopy indicated that there were some large droplets ($d>10\;{\mu}m$) in all FG-emulsions, nevertheless, the amount of large droplets tended to decrease with increasing FG concentration. More than 90% of FG was present free in the continuous phase of the emulsions. To facilitate droplet disruption and prevent droplet coalescence within the homogenizer, homogenization time was adjusted in O/W emulsions stabilized by 2.0 or 4.0 wt% FG. However, the increase in the number of pass rather promoted droplet coalescence. This study has shown that the FG may have some limited use as a protein emulsifier in O/W emulsions.