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Anesthetic Effect and Physiological Response in Olive Flounder (Paralichthys olivaceus) to Clove Oil in a Simulated Transport Experiment

  • Gil, Hyun Woo (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Ko, Min Gyun (Institute of Marine Living Modified Organism (iMLMO), Pukyung National University) ;
  • Lee, Tae Ho (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Park, In-Seok (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Kim, Dong Soo (Institute of Marine Living Modified Organism (iMLMO), Pukyung National University)
  • Received : 2016.08.03
  • Accepted : 2016.09.20
  • Published : 2016.09.30

Abstract

The optimum concentrations of clove oil as an anesthetic for olive flounder (Paralichthys olivaceus) and the stress response of the fish to clove oil anesthesia were determined over a range of water temperatures, and investigated in a simulated transport experiment using analysis of various water and physiological parameters. While the time for induction of anesthesia decreased significantly as both the concentration of clove oil and water temperature increased, the recovery time increased significantly (P<0.05). The plasma cortisol concentration in fish at each temperature increased significantly up to 12 h following exposure (P<0.05), then decreased to 48 h (P<0.05). The DO dissolved oxygen concentrations, pH values, and the fish respiratory frequencies decreased over 6 h following exposure to clove oil in all experimental groups (P<0.05), whereas the $NH_4{^+}$ and $CO_2$ concentrations in all experimental groups increased up to 6 h (P<0.05). The pH values and DO concentrations increased with increasing clove oil concentration (P<0.05) in the 6 h following exposure, and the $CO_2$ and $NH_4{^+}$ concentrations and the respiratory frequencies decreased with increasing clove oil concentration (P<0.05). The results of this experiment suggest that clove oil reduced the metabolic activity of olive flounder, thus reducing $NH_4{^+}$ excretion and $O_2$ consumption. In conclusion, clove oil appears to be a cost-effective and efficient anesthetic that is safe for use and non-toxic to the fish and users. Its use provides the potential for improved transportation of olive flounder.

Keywords

References

  1. Barton BA, Iwama GK (1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis 1:3-26. https://doi.org/10.1016/0959-8030(91)90019-G
  2. Carmichael GJ, Tomasso JR (1988) Survey of fish transportation equipment and techniques. Prog Fish-Cultur 50:155-159. https://doi.org/10.1577/1548-8640(1988)050<0155:CSOFTE>2.3.CO;2
  3. Carmichael GJ, Tomasso JR, Simco BA, Davis KB (1984) Characterization and alleviation of stress associated with hauling largemouth bass. Trans Amer Fisher Soc 113:778-785. https://doi.org/10.1577/1548-8659(1984)113<778:CAAOSA>2.0.CO;2
  4. Constantinos CM, Gloriana C, Irini S, Alberta PM (2005) Comparative efficacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures. Aquaculture 246:467-481. https://doi.org/10.1016/j.aquaculture.2005.02.046
  5. Ferreira JT, Schoonbee HJ, Smith GL (1984) The use of benzocaine-hydrochloride as an aid in the transport of fish. Aquaculture 42:169-174. https://doi.org/10.1016/0044-8486(84)90364-8
  6. Grizzle JM, Mauldin AC, Young D Henderson E (1985) Survival of juvenile striped bass (Morone saxatilis) and Morone hybrid bass (Morone crysops $\times$ Morone saxatilis) increased by addition of calcium to soft water. Aquaculture 46:167-171. https://doi.org/10.1016/0044-8486(85)90201-7
  7. Guo F-C, Teo L-H, Chen T-W (1995) Effects of anaesthetics on the water parameters in simulated transport experiment of playfish, Xiphophorus maculatus (Gunther). Aquacult Res 26:265-271. https://doi.org/10.1111/j.1365-2109.1995.tb00911.x
  8. Han KH, Kim K-S, Lee S-H (2007) Morphological studies on the digestive tracts of the larvae and juveniles of the flounder, Paralichthys olivaceus. Dev Reprod 11:121-125 (in Korean with English abstract).
  9. Hur JW, Park I-S, Chang YJ (2007) Physiological responses of the olive flounder, Paralichthys olivaceus, to a series stress during the transportation process. Ichthyol Res 54:32-37. https://doi.org/10.1007/s10228-006-0370-2
  10. Massee KC, Rust MB, Hardy RW, Stickney RR (1995) The effectiveness of tricaine, quinaldine sulfate and metomidate as anesthetics for larval fish. Aquaculture 134:351-359. https://doi.org/10.1016/0044-8486(95)00057-9
  11. Mommsen TP, Vijayan MM, Moon TW (1999) Cortisol in teleosts: Dynamics, mechanisms of action and metabolic regulation. Rev Fish Biol Fisher 9:211-268. https://doi.org/10.1023/A:1008924418720
  12. Park I-S, Gil HW, Yoo GY, Oh JS (2015) Effects of starvation in rock bream, Oplegnathus fasciatus and olive flounder, Paralichthys olivaceus. Dev Reprod 19:97-109. https://doi.org/10.12717/DR.2015.19.2.097
  13. Park I-S, Hur JW, Choi JW (2012) Hematological responses, survival, and respiratory exchange in the olive flounder, Paralichthys olivaceus, during starvation. Asian-Aust J Anim Sci 25:1276-1284. https://doi.org/10.5713/ajas.2012.12128
  14. Park I-S, Jo JH, Lee SJ, Kim YA, Kim KE, Hur JW, Yoo JS, Song YC (2003) Anaesthetic effect of lidocaine hydrochloride-sodium bicarbonate and MS-222 on the greenling (Hexagrammos otakii). J Kor Fish Soc 36:449-453.
  15. Park I-S, Kim J-M, Kim YH, Kim DS (1988) Influence of lidocaine as an anaesthetic for marine fishes. J Fish Pathol 1:123-130 (in Korean with English abstract).
  16. Park I-S, Lim CH, Choi MS (1998) The evaluation of lidocaine-hydrochloride as anaesthetic for the transportation of Rhynchocypris steindachneri. J Kor Fish Soc 31:785-790 (in Korean with English abstract).
  17. Park I-S, Park MO, Hur JW, Kim DS, Chang YJ, Kim YJ, Park JY, Johnson SC (2009a) Anesthetic effects of lidocaine-hydrochloride on water parameters in simulated transport experiment of juvenile winter flounder, Pleuronectes americanus. Aquaculture 294:76-79. https://doi.org/10.1016/j.aquaculture.2009.05.011
  18. Park I-S, Park SJ, Gil HW, Nam YK, Kim DS (2011) Anesthetic effects of clove oil and lidocaine-HCl on marine medaka, Oryzias dancena. Lab Animal 40:45-51. https://doi.org/10.1038/laban0211-45
  19. Park MO, Hur WJ, Im SY, Seol DS, Lee JH, Park I-S (2008) Anaesthetic efficacy and physiological responses to clove oil-anaesthetized kelp grouper Epinephelus bruneus. Aquacult Res 39:877-884. https://doi.org/10.1111/j.1365-2109.2008.01941.x
  20. Park MO, Im S-Y, Seol D-W, Park I-S (2009b) Efficacy and physiological responses of rock bream (Oplegnathus fasciatus) to anesthetization with clove oil. Aquaculture 287:427-430. https://doi.org/10.1016/j.aquaculture.2008.10.049
  21. Raabo E, Terkildsen TC (1960) On the enzymatic determination of blood glucose. Scandin J Clinic Lab Invest 12:402-407. https://doi.org/10.3109/00365516009065404
  22. Schreck CB (1982) Stress and rearing of Salmonids. Aquaculture 28:241-249. https://doi.org/10.1016/0044-8486(82)90026-6
  23. Sladky KK, Swanson CR, Stoskopf MK, Loomis MR, Lewbart GA (2001) Comparative efficacy of tricaine methanesulfonate and clove oil for use as anesthesia in red pacu (Piaractus brachypomus). Am J Vet Res 62:337-342. https://doi.org/10.2460/ajvr.2001.62.337
  24. Staurnes M, Sigholt T, Pedersen HP, Rustad T (1994) Physiological effects of simulated high-density transport of Atlantic cod (Gadus morhua). Aquaculture 119:381-391. https://doi.org/10.1016/0044-8486(94)90302-6
  25. Summerfelt RC, Smith LS (1990) Anesthesia, surgery, and related techniques. In: Methods for Fish Biology, American Fisheries Society, Bethesda, MD, USA, pp 213-272.
  26. Tomasso JR, Davis KB, Parker NC (1980) Plasma corticosteroid and electrolyte dynamics of hybrid striped bass (white bass$\times$striped bass) during netting and hauling stress. Proceed World Maricult Soc 11:303-310.
  27. Woody CA, Nelson J, Ramstad K (2002) Clove oil as an anesthetic for adult sockeye salmon (Oncorhynchus nerka): field trials. J Fish Biol 60:340-347. https://doi.org/10.1111/j.1095-8649.2002.tb00284.x