• 한국해양바이오학회지
• /
• 제6권2호
• /
• pp.123-130
• /
• 2014

Three types of artificial microparticle diets were developed for rotifer (Brachionus plicatilis) enrichment. The efficacies of enrichment with the artificial diets were evaluated and compared to those with commercial enrichment diets on the growth and survival of flounder larvae. Total lipid content was highest in the rotifer enriched with oil capsule (40.5% in dry weight). The n-3 highly unsaturated fatty acid (n-3 HUFA) content was also highest in the rotifer fed with oil capsule (7.08% in dry weight). The flounder larvae fed on the rotifer enriched with oil capsule showed the highest growth compared to those fed on any other enriched rotifer (P<0.05). The survival ratio of flounder larvae fed on the rotifers enriched with oil capsule and emulsion oil were higher than those fed on any other enriched rotifer (P<0.05). From the feeding study, the growth and survival of flounder larvae were enhanced by feeding rotifer enriched with oil capsule compared to rotifer enriched with any other diets. The rotifer fed on oil capsule containing high contents of n-3 HUFA. Therefore, a significant relationship between the growth and survival of flounder larvae and the n-3 HUFA content of rotifer could be obtained.

• Fisheries and Aquatic Sciences
• /
• 제1권1호
• /
• pp.30-34
• /
• 1998

Rotifer culture fed on five types of artificial microparticle diets were evaluated to substitute the natural diets such as Chlorella or w-yeast. These microparticle diets including solidified blood using squid oil (SBSO), solidified blood using soybean oil (SBSB), nylon protein walled particle (NPW) simple coacervation oil capsule (SCO), complex coacervation oil capsule(CCO), were tested for the evaluation of feeding efficiency. The prepared micro particle diets had diameters ranging from 3 to 30 Jim. Rotifer culturing experiments were carried out in 3-liter beakers for 13-16 days. The initial inoculum density of rotifers was 10 ind./ml. The rotifers fed on Chlorella or $\omega-yeast$ showed maximal densities of 2,000 ind./ml in 9 days or 500 ind./ml in 7 days, respectively. Those fed on SBSO, SBSB or NPW showed maximal densities of 1568 ind./ml, 586 ind./ml or 503 ind./ml, respectively and the reproductive rates for those diets were equivalent to or better than w-yeast. However, the coacervated oil capsule showed lower maximal densities of 400 ind./ml for SCO and less than 100 ind./ml for CCO due to the unbalanced diet formulation and indigestibility.

• 한국식품영양학회지
• /
• 제25권4호
• /
• pp.1027-1032
• /
• 2012

본 연구에서는 혈액 내에서의 오메가-3 지방산의 함량 변화를 측정하여 연질 캡슐과 장용성 캡슐의 차이를 검토하였다. Fish oil 중의 EPA와 DHA의 총합의 함량이 62.87 g/100 g으로 대부분을 차지하고 있다. 연질 캡슐의 경우, 제 1시험액(위액)에서는 내부의 지방이 70% 용출된 반면, 장용성의 경우 10% 정도만이 용출되었다. 제 2액(소장액)에서도 6시간까지 50% 정도 용출되어 90분에 50%가 용출된 일반 연질 캡슐과 대조적이었다. 기존에 사용하였던 연질 캡슐의 EPA 및 DHA는 흡수 초기에 장용성 캡슐에 비하여 다소 높은 비율을 보인 반면, 8시간 이후 장용성 캡슐 섭취한 경우 EPA와 DHA의 혈액내 함량이 비율이 증가하는 경향을 보였다. 경구 투여 48시간 후 지방산 조성은 장용성 캡슐에서는 mono-, poly-unsaturated fatty acid 혈액 내의 함량이 다소 높은 경향을 보였으며, saturated fatty acid 함량 역시 다소 높은 경향을 보였다. EPA와 DHA의 함량은 혈액 내에서 각 지방 분획에서의 함량 차이를 정확하게 측정하기 힘들었다. 이상의 결과에 의하면 장용성 캡슐은 위장관을 통과하여 장에서 용출되는 특성 때문에 지속적인 효과를 기대할 수 있을 것으로 추정된다.

• 한국수산과학회지
• /
• 제48권5호
• /
• pp.589-595
• /
• 2015

Alginates are used to encapsulate various materials, including food, cosmetics, and pharmaceuticals. This study examined the properties and oxidation stability of fish oil capsules manufactured with calcium alginate gels. The fish oil capsules were manufactured by dropping sodium alginate solution and fish oil into a calcium chloride solution through nozzles. The membrane thickness, sphericity, rupture strength and deformation depth of the fish oil capsules were determined. The peroxide value of the fish oil was assayed to determine the oxidation stability of the capsules. The capsules measured approximately 3 mm with a membrane thickness of 90 μm independent of the amount of fish oil added. As the amount of fish oil encapsulated increased, the sphericity, rupture strength and deformation depth of the capsules decreased. The encapsulation efficiency increased until the amount of fish oil was 30%. The oxidation stability of fish oil in capsules was dependent on the type of nozzle, e.g., the oxidation stability of fish oil in capsules made using a double nozzle was greater than with a single nozzle. These results should lead to industrial application of fish oils including eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, as nutraceuticals.

• 한국자원식물학회지
• /
• 제1권1호
• /
• pp.1-7
• /
• 1988

This experiment were carried out to study the heritability, genotypic, pheno-typic and environmental correlation in four edible oil crops. Heritabilities of flowering date, maturing date, plant height and weight of 1, 000 grains in rape were high. A highly significant positive correlation was found between flowering date and maturing date, plant hight and ear length, number of pods per ear and flowering date, maturing date and plant hight, The number of seed per pod showed also significant positive correlation with flowering or maturing date as well as with the relationship between weight of 1, 000 grains and plant height or ear length inrapeseed, respectively. Heritabilities of maturing date, length of stem with eapsule and number of seed per capsule were high, in sesame. Genotypic correlation between plant height and length of stem with capsule, length of stem with capsule and number of capsule per plant, number of capsule per plant and weight of 1, 000 grains, weight of 1, 000 grains and yield were highy positive in sesame. Heritabilities of flowering date, length of main stem, weight of 100 grains and number of shells per square meter in peanut were high, There was positive genotypic correlation between length of main stem and yield, number of shells per quare meter and matured seed ratio, number of shells per square meter and yield, 100 grains weight and yield. On the other hand, a significant negative correlation appeared between flowering date and yield. Heritabilities of days to flowering, stem length, stem diameter and weight of 1, 000 grains in perila were high. There was positive genotypic correlation between stem length and stem diameter, number of pods per plant, stem weight etc, between number of internodes and number of pods per plant, stem weight, number of valid branches and number of pods plant as well, respectively. While, a significant negative correlation was observed between number of valid branches and weight of 1, 000 grains, between number of pods per plant and weight of 1, 000 grains.

• 폴리머
• /
• 제29권4호
• /
• pp.369-374
• /
• 2005

본 실험에서는 레몬 오일을 함유한 알긴산 칼슘 마이크로캡슐을 에멀션-내부 젤화법으로 제조하였으며, 방출실험을 통하여 아로마테라피의 가능성에 대해 살펴보았다. 적외선분광분석(FT-IR)과 시차주사열량계(DSC)를 통해 레몬 오일의 봉입 여부를 확인하였고, 제조된 마이크로캡슐의 직경 및 형태를 주사전자현미경(SEM)으로 관찰하였다. 제조된 마이크로캡슐은 평균 $4\~7$um직경 및 $50\~85\%$의 캡슐화를 보였으며, $37^{circ}C$에서 적외선 수분계(IMDB)를 통한 방출실험 결과. 알긴산 및 $CaCl_2$의 농도가 증가할수록 가교밀도가 증가하므로 레몬 오일의 방출률이 감소함을 관찰할 수 있었다. 또한, 물리적 압력을 통한 방출실험을 실시함으로써, 캡슐벽의 붕괴와 방출속도가 외부적인 요인에 의해 방출속도를 제어할 수 있음을 확인하였다.

• 한국작물학회지
• /
• 제54권1호
• /
• pp.24-28
• /
• 2009

This study was conducted to evaluate the growth characters and yield components of 18 collected sesame cultivars to get basic information on the variation for the sesame breeding using principal component analysis. All characters except days to flowering, days to maturity and 1,000 seed weight showed significantly different. Seed weight per 10 are showed higher coefficient of variance. Capsule bearing stem length and liter weight showed positive correlation with seed yield per 10 are. The principal components analysis grouped the estimated sesame cultivars into four main components which accounted for 83.7% of the total variation at the eigenvalue and its contribution to total variation obtained from principal component analysis. The first principal component ($Z_1$) was applicable to increase plant height, capsule bearing stem length and 1,000-seed weight. The second principal component ($Z_2$) negatively correlated with days to flowering and maturity by which it was applicable to shorten flowering and maturity date of sesame. At the scatter diagram, Yangbaek, Ansan, M1, M2, M4, M7 and M9 were classified as same group, but M10, Yanghuk, Kanghuk, M5, M6, M12 and M13 were classified as different group. This results would be helpful for sesame breeder to understand genetic relationship of some agronomic characters and select promising cross lines for the development of new sesame variety.

• 한국식품영양과학회지
• /
• 제28권4호
• /
• pp.890-894
• /
• 1999

Microencapsulation of anchovy oil as a core material in sodium alginate as a wall material was inves tigated. Microencapsulation was accomplished by injecting an oil/water emulsion, consisting of a mixture of liquefied sodium alginate and emulsifier, under high pressure through an orifice submerged in a calcium lactate solution. Microcapsules suspended in a dispersion fluid were observed under a fluorescence mi croscope to verify the presence of the capsules and to note coalescence or degradation of the capsules. Optimum conditions for microencapsulation of anchovy oil were obtained when 1.0% aqueous solution of sodium alginate contained 3% of a 1:1 ratio of ESPR 25(polyglycerine＋polylinoleate) and TW 20(sorbitan laurate＋ethylene oxide) as an emulsifier in terms of capsule size and size distribution, and emulsion stability. The airless sprayer produced microcapsules with a diameter between 15.9 and 73.9 m with different concentration of a wall material. The optimum mixing ratio of wall material to core material was 90:10(wt/wt). 0.2% calcium lactate was appropriate as a dispersion fluid.

• Biomolecules & Therapeutics
• /
• 제10권3호
• /
• pp.202-207
• /
• 2002

The hepato-protective activity of the GODEX (Hepadif-s capsule) has been studied in the rats against $CCl_4$-ethanol induced liver toxicity. The rats were oral1y treated with $CCl_4$ (corn oil/ $CCl_4$ 1:1, 1 mg/kg). And one week passes, $CCl_4$(0.4 mg/kg) administered two times a week for 7 weeks. The drugs have been administered every two days for 4 weeks after $CCl_4$ injection. The experimental groups have consisted of the GODEX (250 mg/kg), Hepadif (200 mg/kg), DDB complex (DDB 50 mg/kg and garlic oil powder 50 mg/kg), DDB (50 mg/kg), and vehicle control respectively. There was a significant decrement on the serum level of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total bilirubin in all treated groups. Specially, ALT level of GODEX and Hepadif only treated groups was decreased c1early. Also, serum albumin level was significantly enhanced in GODEX treated group compared with control and DDB treated groups. In histological results, hepatocellular vacuolar degeneration, lobular restructure and necrosis of bile duct were severely showed in control. But other treated groups showed centerilobular degeneration and mild hyper-plasia. Hepadif or DDB has a effects of the recovery on serum parameters and structure ill liver injury. When it was compared GODEX to Hepadif alone or DDB complex or DDB, it suggested to have the best activity of the liver recovery.

• 한국응용과학기술학회지
• /
• 제32권4호
• /
• pp.694-701
• /
• 2015

In order to get stabilized pure retinol in skin care cosmetics, developing the three layered matrix bead capsules were studied. This study relates to make a cosmetic composition using the three layered matrix capsule that could increase the stability of the active ingredient. A primary encapsulation, vitamin A (pure retinol) of active ingredient was perfectly capsulated into water-in-oil (Water-in-Oil: W/O) emulsion vesicle using PEG-10 dimethicone copolyol emulsifier. A secondary encapsulation of multiple emulsion of the water-in-oil-in-water (W/O/W) emulsion blending W/O emulsion using sucrose distearate of surfactant was developed using homogenizing emulsifying system. Pure retinol of active ingredient was stably capsulized to inside the W/O/W-multiple emulsion in order to load the triple matrix capsulation. By coating it with a polymer matrix base, encapsulated in the triple layered type, which were developed bead encapsulation of 2~10mm uniformly size. To show beautifully appearance capsulated bead type, these finish particles in this triple matrix layer were developed as a gold, green, dark brown, silver and blue color were encapsulated in the bead types. Structural particle certification of triple matrix layer was observed through SEM analysis. Stability of pure retinol was remained stable more than 99.7% for 30 days at $42^{\circ}C$ incubating conditions compared with non-capsule. This technology was applied in different formulations such as various sizes and colors that by applying the skin care cosmetics. In the future, this technology to encapsulate an unstable active ingredient, we expect to be expanded this application in the food and drug as a time delivery system.