• Molecules and Cells
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• v.27 no.6
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• pp.689-695
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• 2009

Chemically synthesized small interfering RNAs (siRNAs) can specifically knock-down expression of target genes via RNA interference (RNAi) pathway. To date, the length of synthetic siRNA duplex has been strictly maintained less than 30 bp, because an early study suggested that double-stranded RNAs (dsRNAs) longer than 30 bp could not trigger specific gene silencing due to the induction of non-specific antiviral interferon responses. Contrary to the current belief, here we show that synthetic dsRNA as long as 38 bp can result in specific target gene silencing without non-specific antiviral responses. Using this longer duplex structure, we have generated dsRNAs, which can simultaneously knock-down expression of two target genes (termed as dual-target siRNAs or dsiRNAs). Our results thus demonstrate the structural flexibility of gene silencing siRNAs, and provide a starting point to construct multifunctional RNA structures. The dsiRNAs could be utilized to develop a novel therapeutic gene silencing strategy against diseases with multiple gene alternations such as viral infection and cancer.

• Journal of Advanced Navigation Technology
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• v.26 no.5
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• pp.350-357
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• 2022

With the recent prediction of 'Cycle 25', the importance of space weather research increases. Accordingly, the World Radiocommunication Conference (WRC)-23 has adopted Agenda Item 9.1a) and carried out sharing researches between active/passive space weather observation systems and existing services. Therefore, in this paper, in order to increase the precision of space weather environment data and secure the frequency spectrum for observation systems, the direct interference effect from the weather radar in Gosan, Jeju on the space weather observation receiver in Hallim, Jeju, and the indirect interference on the observation receiver by diffraction and scattering from the radar target have been analyzed. As a result, it can be known that the radar direction, the propagation direction diffracted and scattered from the target, and the Rradar Cross Section (RCS) of the radar target, the reception area of the space weather observation antenna, and the antenna off-boresight are important parameters for the interference effect analysis.

• Progress in Medical Physics
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• v.25 no.4
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• pp.288-297
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• 2014

Dynamic conformal arc therapy (DCAT) and flattening-filter-free (FFF) beams are commonly adopted for efficient conformal dose delivery in stereotactic body radiation therapy (SBRT). Off-axis geometry (OAG) may be necessary to obtain full gantry rotation without collision, which has been shown to be beneficial for peripheral targets using flattened beams. In this study dose distributions in OAG using FFF were evaluated and the effect of mechanical rotation induced uncertainty was investigated. For the lateral target, OAG evaluation, sphere targets (2, 4, and 6 cm diameter) were placed at three locations (central axis, 3 cm off-axis, and 6 cm off-axis) in a representative patient CT set. For each target, DCAT plans under the same objective were obtained for 6X, 6FFF, 10X, and 10FFF. The parameters used to evaluate the quality of the plans were homogeneity index (HI), conformality indices (CI), and beam on time (BOT). Next, the mechanical rotation induced uncertainty was evaluated using five SBRT patient plans that were randomly selected from a group of patients with laterally located tumors. For each of the five cases, a plan was generated using OAG and CAG with the same prescription and coverage. Each was replanned to account for one degree collimator/couch rotation errors during delivery. Prescription isodose coverage, CI, and lung dose were evaluated. HI and CI values for the lateral target, OAG evaluation were similar for flattened and unflattened beams; however, 6FFF provided slightly better values than 10FFF in OAG. For all plans the HI and CI were acceptable with the maximum difference between flattened and unflattend beams being 0.1. FFF beams showed better conformality than flattened beams for low doses and small targets. Variation due to rotational error for isodose coverage, CI, and lung dose was generally smaller for CAG compared to OAG, with some of these comparisons reaching statistical significance. However, the variations in dose distributions for either treatment technique were small and may not be clinically significant. FFF beams showed acceptable dose distributions in OAG. Although 10FFF provides more dramatic BOT reduction, it generally provides less favorable dosimetric indices compared to 6FFF in OAG. Mechanical uncertainty in collimator and couch rotation had an increased effect for OAG compared to CAG; however, the variations in dose distributions for either treatment technique were minimal.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.205-216
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• 2019

Since the RNA interference (RNAi) had been discovered in many organisms, small non-coding RNA-mediated gene silencing technology, including RNAi have been widely applied to analysis of gene function, as well as crop improvement. Despite the usefulness of RNAi technology, RNAi transgenic crops have various potential environmental risks, including off-target and non-target effects. In this study, we developed methods that can be effectively applied to environmental risk assessment of RNAi transgenic crops and verified these methods in 35S::dsRNAi_eGFP rice transgenic plant we generated. Off-target genes, which can be non-specifically suppressed by the expression of dsRNAi_eGFP, were predicted by using the published web tool, pssRNAit, and verified by comparing their expressions between wild-type (WT) and 35S::dsRNAi_eGFP transgenic rice. Also, we verified the non-target effects of the 35S:: dsRNAi_eGFP plant by evaluating horizontal and vertical transfer of small interfering RNAs (siRNAs) produced in the 35S::dsRNAi_eGFP plant into neighboring WT rice and rhizosphere microorganisms, respectively. Our results suggested that the methods we developed, could be widely applied to various RNAi transgenic crops for their environmental risk assessment.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.47 no.3
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• pp.187-194
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• 2019

The off-axis Infra-Red(IR) seeker is mounted on the nose cone side of the anti-air high speed missile to alleviate thermal shield effect due to aerodynamic heating. The seeker output can not be regarded as the Line-of-Sight(LOS) rate any more as missile's roll motion to keep the target tracking is associated. In this paper, we propose a method to calculate the LOS rate for off-axis seeker on a 2-axis gimbal. Firstly, true LOS rate equations are analytically derived but not implementable because boresight error rate is not measurable. And then the first order lag approximation to obtain boresight error rate is proposed. The proposed LOS rate calculation method can compensate the coupling effect by considering the rotations of missile and gimbal. The performance of the proposed method is verified via full nonlinear 6-DOF(Degree of Freedom) simulations.

• Physical Therapy Korea
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• v.13 no.4
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• pp.23-29
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• 2006

The purpose of the present study was to examine gaze effects on spatial and kinematic characteristics during a pointing task. Subjects were asked to watch and point to an aimed target (2 mm in diameter) displayed on a vertically mounted board. Four gaze conditions were developed as combinations of "seeing-aiming" in terms of the eye movements: Focal-Focal (F-F), Focal-Fixing (F-X), Fixing-Focal (X-F), and Fixing-Fixing (X-X). Both the home target and an aimed target were presented for 1 second and then were disappeared in F-F and X-F. In X-F and X-X, only an aimed target disappeared after 1 second. Subjects were asked to point (with index finger tip) to an aimed target accurately as soon as the aimed target was removed. A significant main effect of gaze was found (p<.01) for normalized movement time. Peripheral retina targets had significantly larger absolute error compared to central retina targets on the x (medio-lateral) and z (superior-inferior) axes (p<.01). A significant undershooting to peripheral retina targets on the x axis was found (p<.01). F-F and X-F had larger peak velocities compared to F-X and X-X (p<.01). F-F and X-F were characterized by more time spent in the deceleration phase compared to F-X and X-X (p<.01). The present study demonstrates that central vision utilizes a form of on-line visual processing to reach to an object, and thus increases spatial accuracy. However, peripheral vision utilizes a relatively off-line visual processing with a dependency on proprioceptive information.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.7
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• pp.115-121
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• 2013

We fabricated thin film transistors (TFTs) using TiInZnO(TiIZO) thin films as active channel layer. The thin films of TiIZO were deposited at room temperature by RF-magnetron co-sputtering system from InZnO(IZO) and Ti targets. We examined the effects of titanium addition by X-ray diffraction, X-ray photoelectron spectroscopy and the electrical characteristics of the TFTs. The TiIZO TFTs were investigated according to the radio-frequency power applied to the Ti target. We found that the transistor on-off currents were greatly influenced by the composition of titanium addition, which suppressed the formation of oxygen vacancies, because of the stronger oxidation tendency of Ti relative to that of Zn or In. A optimized TiIZO TFT with rf power 40W of Ti target showed good performance with an on/off current ratio greater than $10^5$, a field-effect mobility of 2.09 [$cm^2/V{\cdot}s$], a threshold voltage of 2.2 [V] and a subthreshold swing of 0.492 [V/dec.].

• Journal of Positioning, Navigation, and Timing
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• v.4 no.2
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• pp.57-65
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• 2015

Global Navigation Satellite System (GNSS) including Global Positioning System (GPS) is an important element for navigation of both the military and civil Unmanned Aerial Vehicle (UAV). Contrary to the military UAVs, the civil UAVs use the civil signals which are unencrypted, unauthenticated and predictable. Therefore if the civil signals are counterfeited, the civil UAV’s position can be manipulated and the appropriate movement of the civil UAV to the target point is not achieved. In this paper, spoofing on the autonomous navigation UAV is implemented through field experiments. Although the demanded conditions for appropriate spoofing attack exists, satisfying the conditions is restricted in real environments. So, the Way-point of the UAV is assumed to be known for experiments and assessments. Under the circumstances, GPS spoofing signal is generated based on the Software-based GNSS signal generator. The signal is emitted to the target UAV using the antenna of the spoofer and the effect of the signal is analyzed and evaluated. In conclusion, taking the UAV to the target point is hardly feasible. To implement the spoofing as expectation, the position and guidance system of the UAV has to be known. Additionally, the GPS receiver on the UAV could be checked whether it appropriately tracks the spoofing signal or not. However, the effect of the spoofing signal on the autonomous UAV has been verified and assessed through the experimental results. Spoofing signal affects the navigation system of the UAV so that the UAV goes off course or shows an abnormal operation.

• International journal of advanced smart convergence
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• v.7 no.1
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• pp.33-41
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• 2018

This study was designed to provide basic data for developing exercise program that helps correcting posture by knowing the effect of strengthening and elongation exercises of upper extremity muscle to forward head posture correction. In this study determined subjects whether they had forward head posture or not. On the basis of the New York state posture rating, if a subject's posture is match up with the normal standard posture, gives 5 points and if the posture is slightly get out of the normal standard posture, gives 3 points and if the posture is apparently get out of the standard, gives 1 points. When determining the forward head posture, if talus, humerus and outer ear center are on the same line, it is determined as normal and if outer ear center is off the line less than 1.0cm, it is a slight deformation and if outer ear center is off the line more than 1.0cm, it is a high deformation. In the study selected people who have more than 1 cm gap between two vertical lines start from outer ear center and acromion separately as subjects. Length between the ideal alignment line measured by using goniometer and temporal region showed statistically significant decrease as $2.36{\pm}1.07cm$ before the intervention and $1.06{\pm}0.88cm$ after the intervention. After 4 weeks of neck and chest extensor muscle exercise, the group who exercised both showed increase in range of neck joint motion and neck flexion of the forward head posture. Meanwhile the group who only exercised neck extensor muscle only and the group who only exercised chest extensor muscle didn't showed statistically significant result. That only the group who exercised both muscles showed significant result is the different with studies before. Because this study didn't target patient who had a lesion, couldn't compare effect of the conservative manner and exercise. However, this study provides the fact that the group who exercised both neck and chest muscle had more effect than the control group.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.5A
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• pp.440-446
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• 2010

Convergence of IT and BT is considered in many area, especially in medical care industry. The example of this trend is a capsule endoscope. But in a capsule endoscope, communication through human body has a few restrictions. At first, the transmit power should be limited not to have a bad effect on human organs and for the battery capacity. Second, the channel characteristic of human body has not been examined exactly. Third, general modulation / demodulation techniques which require a channel estimation cannot be used because of battery limit. There also may be a lot of interference signals because a capsule endoscope uses UWB bandwidth. In this paper, we introduce Chirp Spread Spectrum Differential Binary Phase Shift Keying(CSS-DBPSK) and propose Chirp Spread Spectrum On-Off Keying(CSS-OOK) which don't require a channel estimation and robust to interference signals. Using CSS-DBPSK or CSS-OOK, we can get 5 dB or 2~3 dB of Eb/N0 gain at 10-5 target BER. And if there are interference signals, those gains of CSS-DBPSK and CSS-OOK are increased.