• Korean Journal of Microbiology
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• v.40 no.3
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• pp.183-188
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• 2004

Stenotrophomonas sp. OK-5 capable of degrading TNT has been found to have three nitroreductase fractions designated as NTR fractions I, II, and III. NTR in a previous study. This study was attempted to reveal physiological and molecular characteristics of NTR fractions I, II, and III in strain OK-5. Several chemicals (e.g., EDTA, NaCl, dithiothreitol, $\beta$-mercaptoethanol) were tested for their effect on enzyme activity of NTRs, demonstrating that enzyme activities of NTR fractions I, II, and III from OK-5 were inhibited in the presence of $\beta$-mercaptoethanol. Substrate specificity test showed that NTR fractions I, II, and III all have over 70% enzyme activities for nitrobenzene or RDX as a substrate. N-terminal amino acid sequence of NTR fraction I from Stenotrophomonas sp. OK-5 was $^1MSDLLNADAVVQLFRTARDS^20$ and exhibited 70% sequence homology with that of NTR from Xanthomonas campestris. NTR I gene from Stenotrophomonas sp. OK-5 (SmOK5nrI) shared extensive sequence homology in deduced amino acid sequence of PCR product with NTRs from Xanthomonas campestris (81 %), X. axonopodis (75%), Streptomyces avermitilis(30%), whereas they had low homology with that from P. putida KT2440 (pnrB) (16%).

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.247-253
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• 2002

The cellular responses of TNT-degrading bacterium, Stenotrophomonas sp. OK-5 to explosive 2,4,6-trini-trotoluene (TNT) as an environmental contaminant were examined. Survival of the strain OK-5 with time in the presence of different concentrations of TNT under sublethal conditions was monitored, and viable counts paralleled the production of the stress shock proteins in this bacterium. Total cellular fatty acids analysis showed that strain OK-5 produced or disappeared several different kinds of lipids when grown on TNT media than when grown on TSA. Under scanning electron microscope, the cells treated with 0.5 mM TNT for 12 hrs showed irregular rod shapes with wrinkled surfaces. Analyses of SDS-PAGE and Western blot using anti-DnaK and anti-GroEL revealed that several stress shock proteins including 70 kDa DnaK and 60 kDa GroEL in strain OK-5 were newly synthesized at different TNT concentrations in exponentially growing cultures. 2-D PAGE of soluble protein fractions from the culture of OK-5 exposed to TNT demonstrated that approximately 300 spots were observed on the silver stained gel ranging from pH 3 to pH 10. Among them, 10 spots significantly induced and expressed in response to TNT were selected and analyzed. As the result of internal amino acid sequencing with ESI-Q TOF, two proteins, spot #1 and spot #10 were assigned the DnaK protein XF2340 of Xylella fastidiosa and stress-induced protein of Mesorhizobium loti, respectively.

• KSBB Journal
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• v.24 no.6
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• pp.556-562
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• 2009

The biological treatment of TNT-containing wastewater, known commonly as pink water, was investigated using a stirred tank reactor with Stenotrophomonas maltophilia OK-5 bacterial culture. S. maltophilia OK-5 exhibited effective degradation of TNT contained in pink water, completely degrading TNT (100 mg/L) within 6 days of incubation. The dark-red brown color derived from Hydride-Meisenheimer complex became more pronounced during the incubation period, which was determined quantitatively. High-pressure liquid chromatography was used to measure residual TNT, which also resolved the metabolic intermediates (i.e., 2,4-dinitrotoluene, 2,6-dinitrotoluene and 2,4-dinitro-6-hydroxytoluene). Gas chromatography-mass spectrometry was used to verify these intermediates. Quantification of the nitroreductase (pnrB) gene isolated from S. maltophilia OK-5 growing in pink water was performed with real-time PCR. The amount of pnrB gene copies increased to $10^3$-fold after 5 days of incubation time.

• KIEAE Journal
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• v.14 no.5
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• pp.29-38
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• 2014

The Purpose of this study is to analyze the investigation of consumer's consciousness for Han-Ok construction of community facilities in an apartment housing. The contents of this study were largely composed of two parts; First was about concept and type of 'New Han-Ok type' public building. Second was about consciousness for Han-Ok construction of community facilities in an apartment housing. The result to this study is as following: 1) the concept of 'New Han-Ok type' public building should not only be defined in terms of cultural property or Han-Ok experience facilities but daily life and practical community. 2) the consciousness for Han-Ok construction of community facilities in an apartment housing was recognized affirmatively more than 75.9%. 3) the strengths of 'New Han-Ok type' public building was as following: daycare centers & small libraries & management office-use of eco materials, senior centers-traditional maintenance, community center-ease of daylight and ventilation. 4) Weaknesses of 'New Han-Ok type' public building was as following: daycare centers-security problem, senior centers-inconvenience of space, community facilities-inconvenience of facilities, small libraries-lack of storage space, management office-inconvenience of moving line. The conscious research for 'New Han-Ok type' public building should succeed for vitalizing for Han-Ok.

• KIEAE Journal
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• v.14 no.5
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• pp.111-116
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• 2014

Han-ok has been forgotten by rapid industrialization, however, recent interest in Korean traditional culture and eco friendly architecture are increasing. Government is also processing various policies and research in Han-ok for historical reasons. Still Han-ok is considered as temporary discover stage not daily space. Moreover, previous weakness of Han-ok made it difficult to supply. There is a need to build new Han-ok style public buildings different from traditional or new Han-ok for revitalization of Han-ok. Moreover, development of new Han-ok style public buildings model are needed for the public which include updated concept and function. From public buildings model development and supply people can visit and explore Han-ok. Therefore Han-ok tradition can be modernized. In addition to this Korean traditional culture and mode of living can be developed and conserved. This research focuses on public buildings of Han-ok supplication and aims design elements and regional trend.

• Tuberculosis and Respiratory Diseases
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• v.60 no.1
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• pp.72-75
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• 2006

Background : This report reviews our experience with persistent air leaks in the peumothorax that were not considered candidates for surgical treatment in order to evaluate the efficacy and risks of the OK-432 plus autoblood or OK-432 pleurodesis. Material & Methods : From March 2004 to July 2005, 8 consecutive patients who had an air leak in the pneumothorax over 5 days and had been treated with OK-432 plus autoblood or OK-432 pleurodesis. The patients were not considered candidates for surgical treatments because the chest CT findings revealed severe chronic lung disease with multiple bullae and/or bullous changes. A prolonged air leak with/without dead space was treated with either OK-432 plus autoblood or OK-432 pleurodesis. The efficacy and side effects of OK-432 pleurodesis were assessed by determining the duration of the air leak, the number of pleurodesis, the patients' symptoms, measurements of the white blood cell count and the c-reactive protein level. Results : All of eight patients were male and the mean age was $72.4{\pm}8.5$. The mean number of pleurodesis was $1.9{\pm}1.1$ and the mean duration of the air leak was $4.6{\pm}4.6days$ after pleurodesis. Side effects after pleurodesis were encountered in 7 patients, which included a chilling sensation in 7 cases, chest pain in 5 cases, headache in 3 cases, local heat sensation in 2 cases, and fever in 1 case. Leukocytosis was observed in 6 patients, and the mean of WBC count and CRP were $14500{\pm}2100$ and $21.9{\pm}11.4mg/dL$, respectively. Conclusion : Either OK-432 plus autoblood or OK-432 pleurodesis has acceptable side effects, and can be considered a treatment option for persistent air leaks in the pneumothorax that are not candidates for surgical treatment.

• KIEAE Journal
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• v.13 no.2
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• pp.3-12
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• 2013

Recently, New Han-ok is in prevail because it has many good qualities as a dwelling. But the New Han-ok dwellers' needs and satisfaction ratio on their houses are not investigated yet. So, surveys on satisfaction ratio for New Han-ok were carried out to know New Han-ok's existing conditions and inhabitant's responses on New Han-ok dwellings. Followings are results. 1) Dwellers response that it is cool in summer and it is cold in winter. So, design and construction method against cold is needed for New Han-ok. 2) Window is the most picking element of New Han-ok for house improvement. 3) Window opening is main control method for cooling in summer. Warming sources in winter are various such as electricity, gas, oil, wood, etc. 4) PVC window structure and glasses were used for New Han-ok's window system as well as wooden window structure and Han-gi. 5) Interviewees are generally satisfied with New Han-ok's exterior view and inner spaces. Almost no humidifier is used. These results could be used for improvement of Han-ok and basic material for New Han-ok design.

• Journal of the Korean Institute of Rural Architecture
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• v.24 no.2
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• pp.21-28
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• 2022

Focusing on the creation of a new han ok, especially a mid-rise hybrid-structured Han-ok, this study proposes a middle-rise (four-story) Han-ok on one and two lots located in Seoun-dong, the existing Han-ok intensive housing site in downtown Cheongju. 1) In terms of layout and function, according to the existing L-shaped Han-ok corresponding to the road and the direction, the parking lots and shops on the 1st floor, the business facility on the 2nd floor, the Han-ok on the 3rd and 4th floors are placed. There are yards, open roof yards, and semi-open Daecheong(大廳, main hall), which can be shared by residents. 2) In terms of structure and form, one or two floors (some 3 floors) are 5.4m square and 5.4×6.6m modules of the RC(Reinforced Concrete) group, and the upper floor reflects the 2.7m module, size and shape of the existing Han-ok. By extending the outer wall of the RC group in the lower floors (1st to 3rd floors) to the wooden exterior of the upper floors (2nd to 4th floors), it is attempted to avoid the awkward appearance of the RC group being exposed to wooden structures. And it is also attempted to reflect the wooden shape and design elements through the elevation elements such as horizontal windows, corner windows, picture frames, and vertical slits. 3) In environmental control and facilities, it is attempted to smooth the ventilation of the building by forming a vertical upward airflow from the dark space of the low floor to the positive of the upper floor. This doubles the effect through a vertical rise of cold air generated in a narrow alleyway, piloti parking lot, and the various voids. In addition to the Daecheong and Numaru(loft) of Han ok, the rooftop yard, the terrace, and the balcony, horizontal natural ventilation is generated through divided doors and transom windows.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.3
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• pp.280-287
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• 1989

In order to know growing habit of the maize bearing tillers, IK//IRI/B68 with tillers was grown and compared with Jinjoo Ok which is not tillering, and several parameters were measured for analysis. The results obtained were summarized as follows. 1. The plant height and fresh weight of IK//IRI/B68 were lower than those of Jinjoo Ok until the tasseling stage of IK/ /IRI/B68, while then plant height and fresh weight of IK//IRI/B68 were higher than those of Jinjoo Ok after the tasseling stage of IK//IRI/B68. 2. The average number of tillers per plant of IK//IRI/B68 at maturity was 3.7 and the average tiller height of IK//IRI/B68 was almost the same as the height of the main stem. 3. The dry weight of IK//IRI/B68 at the early growing stage was lower than the check hybrid Jinjoo Ok, while it was higher than Jinjoo Ok at the later growing stage. 4. The LAI of IK//IRI/B68 after full expansion of the leaves was greater than that of Jinjoo Ok The LAIs of IK//IRI/B68 and Jinjoo Ok were 12.6 and 5.8, respectively. 5. The CGR of Jinjoo Ok was greater than that of IK//IRI/B68 until earey July while the CGR of Jinjoo Ok was lower than that of IK//IRI/B68 as the tillers of IK//IRI/B68 were fully developed. 6. The LAR and RGR of IK//IRI/B68 were greater than those of Jinjoo Ok until mid-July. The RGR of IK//IRI/B68 seemed to be affected by both NAR and LAR. The RGR of Jinjoo Ok seemed to be affected by LAR.

• Clinical and Experimental Pediatrics
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• v.55 no.5
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• pp.177-180
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• 2012

OK-432 (picibanil) is an inactivated preparation of $Streptococcus$ pyogenes that causes pleurodesis by inducing a strong inflammatory response. Intrapleural instillation of OK-432 has recently been used to successfully treat neonatal and fetal chylothorax. Here we report a trial of intrapleural instillation of OK-432 in two preterm infants who were born with hydrops fetalis and massive bilateral pleural effusion. Both cases showed persistent pleural effusion, refractory to conservative treatment, up to postnatal days 26 and 46, respectively. An average of 80 to 140 mL of pleural fluid was drained daily. In case 1, the infant was treated with OK-432 during the fetal period at gestation 28 weeks and 4 days of gestation, but showed recurrence of pleural effusion and progressed into hydrops. Within two to three days after OK-432 injection, the amount of pleural fluid drainage was dramatically decreased and there was no reaccumulation. We did not observe any side effects related to OK-432 injection. We suggest that OK-432 should be considered as a therapeutic option in infants who have persistent pleural effusion for more than four weeks, with the expectation of the early removal of the chest tube and a good outcome.