• 제목/요약/키워드: OIM

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방선균 항생제 고생산 산업균주를 기반으로 한 모델 폴리케타이드의 이종숙주 발현 (Heterologous Expression of a Model Polyketide Pathway in Doxorubicin-overproducing Streptomyces Industrial Mutants)

  • 김혜진;이한나;김응수
    • 한국미생물·생명공학회지
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    • 제40권1호
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    • pp.10-16
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    • 2012
  • 방선균 Streptomyces peucetius OIM ($\underline{O}$verproducing $\underline{I}$ndustrial $\underline{M}$utant)은 반복적인 돌연변이를 통하여 폴리케타이드 항생제인 독소루비신(DXR)의 생산성이 최적화 된 고생산성 산업균주이다. 이 S. peucetius OIM 변이종을 대리의 숙주로 이용하여, 생합경로 크기가 작은 모델 폴리케타이드인 알로에사포나린 II(액티노로딘의 합성경로 유도체)의 생합성 유전자군을 고복제수 플라스미드에 클로닝하여 알로에사포나린 II의 기능적 발현을 확인하여 정량분석을 수행하였다. OIM 균주의 알로에사포나린 II의 생산량은 조절 네트워크가 극대화된 S. coelicolor 변이종 뿐만 아니라 야생형S. peucetius 보다 매우 높은 수준으로 생산되는 것으로 확인되었다. 또한 알로에사포나린 II의 생산 수준은 다운-조절자 $wblA_{spe}$가 제거된 S. peucetius OIM 균주에서 가장 높은것으로 측정되었으며, 이는 합리적으로 유전체를 재설계한 S. peucetius OIM 변이종 균주가 이종의 폴리케타이드 생합성을 높은 수준으로 발현할 수 있는 대리의 숙주로서 충분히 활용 가능함을 보여준다.

A Study on Data Classification of Raman OIM Hyperspectral Bone Data

  • Jung, Sung-Hwan
    • 한국멀티미디어학회논문지
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    • 제14권8호
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    • pp.1010-1019
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    • 2011
  • This was a preliminary research for the goal of understanding between internal structure of Osteogenesis Imperfecta Murine (OIM) bone and its fragility. 54 hyperspectral bone data sets were captured by using JASCO 2000 Raman spectrometer at UMKC-CRISP (University of Missouri-Kansas City Center for Research on Interfacial Structure and Properties). Each data set consists of 1,091 data points from 9 OIM bones. The original captured hyperspectral data sets were noisy and base-lined ones. We removed the noise and corrected the base-lined data for the final efficient classification. High dimensional Raman hyperspectral data on OIM bones was reduced by Principal Components Analysis (PCA) and Linear Discriminant Analysis (LDA) and efficiently classified for the first time. We confirmed OIM bones could be classified such as strong, middle and weak one by using the coefficients of their PCA or LDA. Through experiment, we investigated the efficiency of classification on the reduced OIM bone data by the Bayesian classifier and K -Nearest Neighbor (K-NN) classifier. As the experimental result, the case of LDA reduction showed higher classification performance than that of PCA reduction in the two classifiers. K-NN classifier represented better classification rate, compared with Bayesian classifier. The classification performance of K-NN was about 92.6% in case of LDA.

다국적기업 자회사 현지 직원의 외국인 임직원과의 접촉이 조직일체감에 미치는 영향: 자회사 현지화 수준의 조절효과 (The Effect of MNC subsidiary Host Country Nationals' Contact with Foreign Managers on Organizational Identification: Moderating Effect of MNC subsidiary Localization)

  • 원지선;유치연;이강표
    • 무역학회지
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    • 제46권2호
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    • pp.39-54
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    • 2021
  • The purpose of this study is as follows. First we investigate the relationship between contact with foreign managers (CFM) of host country nationals (HCNs) working at MNC subsidiaries and their organizational identification with MNC (OIM). Second we examine the moderating effect of MNC subsidiary localization (MSL) on the relationship between CFM and OIM. For this purpose, we set hypotheses on the relationship among CFM, OIM, and MSL. To verify theses hypotheses, we conducted hierarchical regression analysis on the data from 374 HCNs in 56 MNC subsidiaries. The results of this study are as follows: Frist, CFM positively affects OIM. Second, there is a significant moderating effect of MSL on the relationship between CFM and OIM. Our findings have critical implications in that a subsidiary-level variable that has a significant impact on organizational identification with MNC is presented and in that specific managerial guidelines for subsidiaries can be drawn.

Three-dimensional reconstruction of polycrystals using a series of EBSD maps obtained from Dual-beam experiments

  • Kim, MinJi;Son, Youngkyun;Lee, Myeongjin;Jeon, Youngju;Lee, Sukbin
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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    • pp.172-172
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    • 2016
  • Dual-beam experiments (Focused ion beam - Orientation mapping microstructure, FIB-OIM) is a widely used experimental tool because this experiments tool available alternates between automated serial sectioning and EBSD with the help of dual beams. We investigated the reconstruction procedure for analysis tool which three-dimensional internal microstructure using Ni superalloy(IN100) and ZrO2. As a results, we observed annealing twin boundary each layer in Ni superalloy(IN100) and fairly isotropic internal microstructure in ZrO2 using marching cubes algorithm. According to these results, this procedure is reconstructed well and we gained ability to arrange the EBSD map and internal microstructure.

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전광 패킷 교환기의 성능 해석을 위한 통합적 모델 (An Integrated Model for the Performance Analysis of All-Oprical Multi-Hop Packet Switches)

  • 정한유;이충근;서승우
    • 한국광학회:학술대회논문집
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    • 한국광학회 2000년도 제11회 정기총회 및 00년 동계학술발표회 논문집
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    • pp.116-117
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    • 2000
  • 전광 네트워크(All-optical Network)에서 중요한 기능을 담당하는 전광 교환기는 전광 교환 소자 (All-optical Switching Element)로 이루어져 있는데, 각 교환 소자들은 스위칭 모듈(SM), 라우팅 제어 처리기(RCP), 헤더 처리 모듈(HPM), 그리고 입/출력 인터페이스 모듈(IIM, OIM)로 이루어져 있다. 스위칭 시스템 내에 도착하는 패킷은 원하는 스위치의 출력단에 도착하기 전에 많은 교환 소자(SE)들을 지나가게 되는데, 이 때 수많은 패킷들이 서로 충돌하게 되며, 이 과정에서 패킷이 손실된다. (중략)

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강소성 가공 공정을 이용한 튜브 형상 재료의 결정립 미세화에 대한 연구 (Development of Ultrafine-Grained Aluminum Tubes Using Severe Plastic Deformation Process)

  • 김경진;윤정환;양동열
    • 대한기계학회논문집A
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    • 제33권10호
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    • pp.1087-1090
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    • 2009
  • A unique process was developed to improve the mechanical properties of a circular tube. In this proposed process, a large amount of strain is applied to the wall of tube, leading to grain refinement in the material. In order to investigate characteristics of microstructural evolution such as the distribution of grain size and misorientation angle during the process, an EBSD OIM analysis was carried out. The analysis confirms that the proposed process can very effectively produce a circular tube with ultrafine-grains.