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Complement-dependent Cytotoxic Crossmatch for Prevention of Hyperacute Rejection in Canine Renal Allografts (개의 신장 동종 이식에서 초급성 거부반응의 예방을 위한 보체의존성 세포독성 DLA 플래스 I 교차 반응)

  • Ghil Heh Myung;Woo Heung-Myung
    • Journal of Veterinary Clinics
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    • v.22 no.4
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    • pp.322-327
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    • 2005
  • DLA class I complement-dependent cytotoxicity (CDC) cross-match method was established to control hyperacute rejections in organ transplantation. The aim of the present study is to investigate if DLA class 1 CDC corssmatch method is effective to prevent hyperacute rejections in canine renal allografts. Seven mongrel dogs of similar age and weight were used. Erythrocyte crossmatch was first performed and only the negatives were used. Among the same blood types, CDC cross-match was performed. Anti-dog serum, Hank's balanced salt solution(HBSS), and tile self-serum was used as a positive-, a negative-, and all auto-control respectively. After the reaction with class I complement, it was stained with eosin and scored by international cytotoxicity scoring system under inverted phase microscope. According to these results, kidneys oi CDC negatives among same blood types were cross-transplanted to observe the incidence of hyperacute rejections. One of four 1.2 B blood type dogs had autoantibodies. here were negative CDC results among each blood type, and also there were negative results between different blood types. Two pairs with the same blood types and negative CDC results underwent allo-transplantation each other. There were no hyperacute rejections. DLA cross-match method studied in this experiment for canine renal allograft can be effective to prevent hyperacute rejections. it may be applicable for the future studies of histocompatibility testing in canine renal allografts.

A Survey of the Adaptation and Preference for South Korean Food in North Korean Refugees (새터민들의 남한음식에 대한 수용과 선호도 조사)

  • Lee, Eun-Jung;Pei, Yong-Qin;Kim, Eun-Mi
    • Culinary science and hospitality research
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    • v.19 no.2
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    • pp.93-110
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    • 2013
  • The purpose of this study was to investigate the adaptation and preference for South Korean food among North Korean refugees. A survey was conducted on 220 North Korean refugees regarding general questions, adaptations, and preference on consuming dishes in South Korea. The results of the survey showed that the average difficulty degree scored with the adaptation to South Korean food was 3.06 points out of 5.00 points. The adaptation to Korean food was investigated as 'average' and some North Korean refugees had some difficulties in South Korean food. Some respondents described South Korean food as having very strong flavor that tastes more sweetened, seasoned, and spicy than North Korean food. The extended residency in South Korea allowed North Korean refugees to become more familiar with sweetened flavors. Most of them enjoy an oily and spicy taste more than South Koreans. North Korean refugees like Ssal bap, Naengmyeon, Doenjang-guk, Galbi-jjim, Bulgogi(beef, pork), Chaesobokkeum, Oi-saengchae, Chaeso-jeon, Baechu-kimchi, Saengsun-twigim, Soondae, pears, fruit juice, and spring water. North Korean refugees over the age of 30 years enjoy classic foods such as Japgokbap, Gimbap, Jumeokbap, Hwedeopbap, and Chobap. On the other hand, North Korean refugees less than 30 years old enjoy new foods like ramen and spaghetti.

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Design & Implementation of a Motion Capture Database Based on Motion Ontologies (온톨로지 기반의 모션 캡처 데이터베이스 설계 및 구현)

  • Chung Hyun-Sook
    • Journal of Korea Multimedia Society
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    • v.8 no.5
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    • pp.618-632
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    • 2005
  • A framework for semantic annotation oi human motion sequences is proposed in this paper. Motion capture technology is widely used for manuiacturing animation since it produces high qualify character motion similar to the actual motion of the human body. However, motion capture has a significant weakness due to the lack of an industry wide standard for archiving and retrieving motion capture data. It is difficult for animators to retrieve the desired motion sequences from motion capture files as there is no semantic annotation on already captured motion data. Our goal is to improve the reusability of motion capture data. To archive our goal first, we propose a standard format for integrating different motion capture file formals. Our standard format is called MCML (Motion Capture Markup Language). It is a markup language based on XML (extensible Markup Language). The purpose of MCML is not only to facilitate the conversion or integration of different formats, but also to allow for greater reusability of motion capture data, through the construction of a motion database storing the MCML documents Second, we define motion ontologies that are used to annotate and semantically organize human motion sequences. This ontology-based approach provides the means for discovering and exploiting the information and knowledge surrounding motion capture data.

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The Relationship among Health Promotion Behaviors, Self-Respect and Life Satisfaction of Middle-aged Men and Women Who use the Life Gym (생활체육관을 이용하는 중년남녀의 건강증진행위, 자아존중감 및 생활만족도와의 관계)

  • Lee, Oi-Sun;Park, Sung-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.327-334
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    • 2016
  • This study was conducted to identify the relationship among health promotion behaviors, self-respect, and life satisfaction of middle-aged men and women who use the life gym. The participants included 208 middle-aged men and women who use the life gym in Korea. The data were collected between July 26 and September 10, 2014, using a self-report questionnaire, and were analyzed by frequencies, t-test, ${\chi}^2$ test, ANOVA, and Pearson's correlation using SPSS Win 18.0. The score for health promotion behaviors was 3.41, with men scoring 3.31 and women scoring 3.47 (t = -2.09, p=.038). Self-realization was the highest and physical activity was the lowest in the sub-region. The score for self-respect was 3.42, with men scoring 3.39 and women scoring 3.34. The life satisfaction score was 3.35, with men scoring 3.34 and women scoring 3.36. Health promotion behavior was positively correlated with self-respect (r = .427, p<.001) and life satisfaction (r= -.760, p<.001). Self-respect showed a significant positive correlation with life satisfaction (r = .469, p<.001). Moreover, higher health promotion behavior resulted in higher self-respect and life satisfaction. Moreover, it was found that higher self-respect led to higher life satisfaction. Therefore, to increase self-respect and life satisfaction of middle-aged men and women, it is necessary to develop and test programs for health promotion behaviors.

Development of self-sealing waterproof materials using GRT powder (폐타이어 분말을 이용한 자체보수성 방수재 개발)

  • Lee Dong-Min;Choi Joong-So
    • Resources Recycling
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    • v.14 no.4 s.66
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    • pp.22-33
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    • 2005
  • Four sheet-shaped and one soft-shaped self-sealing waterproof materials were prepared to recycle some GRT(Ground Rubber Tires). Their physical properties were tested to consider characteristics of them. The self-sealing waterproof materials were consisted of GRT/super absorbent polymer(SAP)/binder and mold by a hot press after mixing with a batch-typed internal mixer. The average size of GRT particles was -40 mesh, SAPs were commercial GE-500F and poly(AM-SAS-AA) prepared in this work. Binders were PU. EVA, LDPE, SBR, and poly(2-EHA). And PU film was attached to improve the properties of waterproof materials. Characteristics of self-sealing waterproof materials consisted of by GRT/GE-500F/EV-600/PU film and GRT/GE-500F/SBR(vulcanization)/PU film among the developed self-sealing waterproof materials were similar to the commercial products. And properties of the soft-shaped self-sealing waterproof materials consisted of by GRT/GE-500F/Po1y(2-EHA) and CRT/Poly(AM-SAS-AA)/poly(2-EHA) were improved within from four times to twenty times compared to the one oi the commercial products.

Influence of Perception of Importance of Communication, Self-esteem and Communication skill on Patient Safety attitude of Nursing student (간호대학생의 의사소통 중요성 인식, 자존감 및 의사소통능력이 환자안전태도에 미치는 영향)

  • Lee, Oi Sun;Lee, Kyoung Sook;Gu, Hye Ja
    • Journal of Digital Convergence
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    • v.18 no.10
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    • pp.307-314
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    • 2020
  • This purpose of the study is to investigate the influence of perception of importance of communication, self-esteem and communication skill on patient safety attitude of nursing students. Subjects were 164 associate nursing students associate nursing students(3·4rd). The data were collected using self -report questionnaire from May 17 to June 14, 2019. Data were analyzed by descriptive statistics, t-test, ANOVA, Pearson correlation and hierarchical multiple regression with SPSS/WIN 23.0. Mean point of perception of importance of communication(5 point scale) was 3.75. self-esteem(4 point scale) was 3.64, and communication skill(5 point scale) was 3.60 and patient safety attitude(5 point scale) was 4.44. Patient safety attitude were positively correlated with perception of importance of communication (r=.57, p<.001), self-esteem(r=.32. p <.001) and communication skill(r=.34. p <.001) of nursing students. Perception of importance of communication(β=.520, p<.001), self-esteem(β=.165, p=.039) explained 34.76% of total variance of patient safety attitude of nursing students. Therefore, To increase patient safety attitude of nursing students, it is necessary to develop and test the program for improving perception of importance of communication and self-esteem of nursing students.

Effects of Nonylphenol on CYP17 and CYP19 Expression in the Ovary of Sprague-Dawley Female Rats (Nonylphenol이 CYP17 및 CYP19발현에 미치는 영향)

  • Kim Hee Jin;Ahn Mee Young;Kim In Young;Kang Tae Seok;Kim Tae Sung;Kang Il Hyun;Moon Hyun Ju;Kil Hoyun;Kim Soon Sun;Lee Rhee Da;Park Kui Lea;Han Soon Young;Kim Hyung Sik
    • Environmental Analysis Health and Toxicology
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    • v.20 no.3 s.50
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    • pp.195-203
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    • 2005
  • Cytochrome P45O 17$\alpha$-hydroxylase (CYPI 7) and cytorhrome P45O aromata.ie (CYPI 9) are key steroidogenic enzymes in androgen and estrogen synthesis. ThiL study evaluated the effects of nonylphenol (NP) on CYP17 and CYP19 expression in the ovary of Sprague-Dawley rats. All female rats were administered orally with the vehicle (control, corn oil), diethylstilbestrol (DES, 5.0 $\mu$g/kg) and NP (50, 100, or 200 mg/kg/day), which was startinB when they were weaned at 21 days of age for 20 days. Twenty four hours after final dose, the animals were anelthetized with ether. Significant decreases in the uterus (wet weight) were observed with 5.0 $\mu$g/kg/day DES (78$\%$, of control) and 200 mg/kg/day NP (62$\%$ of control), respectively Additionally, ovarian weight was significantly decreased with 5.0 $\mu$g/kg/day DES (63$\%$ of control) and 200 mg/kg/day NP (72$\%$ of control). The serum estradiol levels were sligHtly lower in DES and high dose NP treatment groups, but the 74 levels were not affected by DES and NP. The expression of the ovarian CYP19 gene increased with low doses (50 and 100 mg/kg/day) of NP. while DES and high dose oi NP (200 mg/kg/day) did not affect on the CYP19 mRNA levels. In contrast to the CYP19 gene, the CYP17 gene expreLsion level was significantly down-regulated by the DES and 200 mg/ks/day NP. This result suggestE that NP inhibits ovarian estrogen synthelis by supprelsing CYP17 mRNA efprelsion, And different mechanisml might exist for the expression of Lteroidogenic CYP17 and CYP19 genes in the ovary of Sprague-Dawley rats in response to NP.

Study on Preparation of Yogurt from Milk and Rice (우유와 쌀을 이용한 요구르트의 제조에 관한 연구)

  • Hong, Oi-Sook;Ko, Young-Tae
    • Korean Journal of Food Science and Technology
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    • v.23 no.5
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    • pp.587-592
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    • 1991
  • The curd yogurt was prepared from milk or milk added with skim milk powder or four types of rice powder. Acid production by lactic acid bacteria in milk containing additive of 2% (w/v) was investigated and quality of curd yogurt (sensory property and keeping quality) was examined. Some organic acids in curd yogurt were analyzed by HPLC. Four types of rice powder, particularly brown rice, stimulated acid production by lactic acid bacteria more than control (milk yogurt). Among four organisms tested, Leuconostoc mesenteroides and Lactobacillus bulgaricus produced more acid than L. casei and L. delbrueckii. HPLC analysis of organic acids in curd yogurt showed that the amount of lactic acid markedly increased during the fermentation by L. bulgaricus for 24 hours while the amount of citric acid markedly decreased. Addition of rice powders to milk slightly reduced sensory property of curd yogurt. Among four types of rice powder tested, tongil rice added sample showed better sensory acceptability than other samples. When curd yogurt was kept at $5^{\circ}C$ for two weeks, acidity and number of viable cells of curd yogurt were not changed.

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Formulation of Sustained-release Tablets of Felodipine using Hydrophilic Polymers and Non-ionic Surfactants (친수성고분자 및 비이온성 계면활성제를 이용한 펠로디핀 서방정제의 설계)

  • Lee, Jin-Kyo;Yang, Sung-Woon;Lee, Bong-Sang;Jeon, Hong-Ryeol;Lee, Jae-Hwi;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
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    • v.36 no.4
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    • pp.271-276
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    • 2006
  • Felodipine, a calcium-antagonist of dihydropyridine type, is a poorly water soluble drug and has very low bioavailability. As preceding studies, use of solid dispersion systems and surfactants(solubilizers) has been suggested to increase dissolution and to improve bioavailability of felodipine. But in case of solid dispersion systems, large amount of toxic organic solvents should be used and manufacturing process time become longer than conventional process. In case of using surfactants, as time elapsed, decreasing of dissolution rate of felodipine due to crystallization has been reported. In this study, Copovidon as a hydrophilic polymer and $Transcutol^{\circledR}$ as a surfactant were combined to formulations if order to increase dissolution of felodipine and conventional wet granulation process were applied to manufacturing of formulations. The effect of Copovidon and $Transcutol^{\circledR}$ on the dissolution oi felodipine was investigated in-vitro. When Copovidon and $Transcutol^{\circledR}$ used simultaneously, the dissolution rate of felodipine was prominently increased compared with when used separately and the maximum increase in the dissolution of felodipine was 5.8 fold compared to control. This is most probably due to synergy effect by combination of Copovidon and $Transcutol^{\circledR}$. Felodipine sustained release tablets were successfully formulated using several grades of HPMC as a release retarding agent. The stability of felodipine sustained release tablet was evaluated after storage at accelerated condition($40^{\circ}C/75%\;RH$) for 6months in HDPE(High density polyethylene) bottle. Neither significant degradation nor change of dissolution rate for felodipine was observed after 6months. In conclusion, felodipine sustained release tablet was successfully formulated and dissolution of felodipine, poorly water soluble drug, was prominently increased and also stability was guaranteed by using combination system of hydrophilic polymer and surfactant.

A Multistage Authentication Strategy for Reliable N-to-N Communication in CGSR based Mobile Ad Hoc Networks (CGSR 기반의 이동 애드 흑 네트워크에서 신뢰성 있는 통신을 위한 노드간 인증 기법)

  • Lee Hyewon K.;Mun Youngsong
    • Journal of KIISE:Information Networking
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    • v.32 no.6
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    • pp.659-667
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    • 2005
  • A Mobile Ad Hoc Network(MANET) is a multi hop wireless network with no prepared base stations or centralized administrations, where flocks of peer systems gather and compose a network. Each node operates as a normal end system in public networks. In addition to it, a MANET node is required to work as a router to forward traffic from a source or intermediate node to others. Each node operates as a normal end system in public networks, and further a MANET node work as a router to forward traffic from a source or intermediate node to the next node via routing path. Applications of MANET are extensively wide, such as battle field or any unwired place; however, these are exposed to critical problems related to network management, node's capability, and security because of frequent and dynamic changes in network topology, absence of centralized controls, restricted usage on network resources, and vulnerability oi mobile nodes which results from the special MANET's character, shared wireless media. These problems induce MANET to be weak from security attacks from eavesdropping to DoS. To guarantee secure authentication is the main part of security service In MANET because networks without secure authentication are exposed to exterior attacks. In this paper, a multistage authentication strategy based on CGSR is proposed to guarantee that only genuine and veritable nodes participate in communications. The proposed authentication model is composed of key manager, cluster head and common nodes. The cluster head is elected from secure nodes, and key manager is elected from cluster heads. The cluster head will verify other common nodes within its cluster range in MANET. Especially, ID of each node is used on communication, which allows digital signature and blocks non repudiation. For performance evaluation, attacks against node authentication are analyzed. Based on security parameters, strategies to resolve these attacks are drawn up.