• 제목/요약/키워드: OFS

검색결과 31건 처리시간 0.021초

유산균 발효에 의한 손바닥선인장 줄기추출물의 지표물질 함량 변화 분석 (Analysis of Marker Components of Fermented Opuntia ficus-indica var. saboten Stem Extracts)

  • 신동원;이상호;이소연;한은혜
    • 분석과학
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    • 제31권6호
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    • pp.219-224
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    • 2018
  • 손바닥선인장(Opuntia ficus-indica var. saboten)은 제주도 자생식물로 섭취가 가능하고 항염 및 항산화, 인지개선 등 다양한 효능이 보고되었다. 본 연구에서는 손바닥선인장 줄기추출물의 지표물질 함량과 인지개선 효능을 높이기 위해 유산균 발효를 수행하였으며, 비발효추출물과 발효추출물 간 지표물질 함량 차이를 비교하고 acetylcholinesterase (AChE)의 저해 활성도를 평가하여 유산균 발효가 인지장애 개선에 미치는 효과를 연구하였다. 지표물질로는 손바닥선인장 줄기 중 함량이 높은 dihydrokaempferol(DHK)과 항산화 효능이 우수하다고 알려진 quercetin-3-methyl ether (3-MeQ)를 선정하였으며, 함량은 HPLC-PDA (280 nm, 360 nm)로 측정하였다. 실험 결과, 발효추출물의 DHK 함량은 비발효추출물에 비해 16.7 % 증가하였고, 3-MeQ 함량은 L. plantarum과 L. fermentum 발효추출물에서 각각 28.6 %, 21.4 % 증가하였다. 또한, 발효추출물의 AChE 저해 활성이 비발효추출물 ($IC_{50}=28.35mg/mL$)보다 높았으며, 발효추출물 중에서도 L. plantarum 발효추출물 ($IC_{50}=12.56mg/mL$)이 L. fermentum 발효추출물 ($IC_{50}=17.71mg/mL$)보다 높은 AChE 저해 활성을 보였다. 따라서 손바닥선인장 줄기추출물은 유산균 발효에 의해 지표성분 함량이 증가되었으며, AChE 저해 활성이 높아지고 이로 인해 인지장애 개선 효과가 나타난 것으로 사료된다. 이러한 결과는 효능이 향상된 고부가가치의 손바닥선인장 제품 개발에 유용한 자료로 활용될 수 있을 것이다.

수중에서 펄스초음파 신호에 대한 Sagnac 간섭형 광섬유센서의 특성 연구 (A Study on Characteristics of Sagnac Interferometric Optical Fiber Sensor to Pulsed Ultrasonic Signal in Underwater)

  • 이준호;신대용
    • 대한전기학회논문지:전기물성ㆍ응용부문C
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    • 제53권1호
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    • pp.15-19
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    • 2004
  • In this paper, we present the fabrication and characteristic analysis of Sagnac interferometric optical fiber senior(OFS) system for detecting pulsed ultrasonic signal in underwater. The hollow cylindrical mandrel wound round by single mode optical fiber is used as sensing component. The ultrasonic signal source is simulated by the PZT actuator operated by an function generator. The distance dependency of the OFS's sensitivity was measured. The sensitivity has been shown to be inversely propotional to the square-root of distance between ultrasonic source and sensing component. It has also been shown that the OFS could detect the signals less affected by ultrasonic path comparing to conventional acousto-electric sensor. and accurate location of ultrasonic signal could be carried out using two OFSs.

선인전 및 감귤염색에 의한 단백질 직물의 물성 및 색상에 관한 연구 (Effect of Opuntia Ficus-indicts variety Saboten Makino and Satsuma Mandarin Dyeing on Physical Characteristics and Colors of Protein Fabrics)

  • 박순자;박덕자
    • 한국의류학회지
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    • 제26권3_4호
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    • pp.473-484
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    • 2002
  • The physical characteristics of silk and wool fabrics dyed with OFS-FR and SM-FR were examined and color difference and colorfastness of these fabrics were analyzed. The results are as follows; 1. The thickness, density and weight of both dyed fabrics were increased than those of undyed fabrics independently of dyeing materials and mordants. 2. Air permeability of all the silk samples increased than that of undyed silk, on the other hand, air permeability of most of wool samples decreased than that of undyed wool. 3. Thermal insulation values of silk samples were not consistent with dyeing conditions, but those of wool samples increased than those of undyed fabrics regardless of dyeing materials and mordants. 4. Color difference was clear between OFS-FR and SM-FR dyed fabrics. The colors dyed with OFS-FR-Al and OFS-FR-chitos an showed violet, those dyed with SM-FR displayed yellowish, in addition to in case of wool, dyed with OFS-FR-Cu the color was greenish yellow, in case of silk dyed with SM-FR-Cu that was yellowish red. 5. Sense of silk fabrics by sensory-test was soft and smooth. Feeling of colors in silk fabrics was wick, warm and slightly brilliant. In case wool, sense of dyed fabrics was slightly soft and slightly rough. Feeling of colors in wool fabrics was warm but not elegant. Therefore the result of sensory-test in silk might be better than that of in wool. 6. Colorfastness to dry cleaning and perspirations was good, but that to washing and sunlight was not fine.

Response of Soil Microbial Communities to Different Cultivation Systems in Controlled Horticultural Land

  • Lee, You-Seok;Kang, Jeong-Hwa;Choi, Kyeong-Ju;Lee, Seong-Tae;Kim, Eun-Seok;Song, Won-Doo;Lee, Young-Han
    • 한국토양비료학회지
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    • 제44권1호
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    • pp.118-126
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    • 2011
  • Ester-linked fatty acid methyl ester (EL-FAME) profiles were used to describe differences in soil microbial communities influenced by conventional farming system (CFS), and organic farming system (OFS) in controlled horticultural land. Soil physicochemical properties and soil microbial communities were determined in the experimental fields. Higher organic matter content in OFS reduced soil bulk density which in turn increased the soil porosity. Generally, soil chemical properties in OFS were higher than those of CFS, but EC value in OFS was significantly lower than that of CFS. With the exception of Fe content, other macronutrient contents and pH in both farming system decreased with the soil depth. Soil microbial biomass of OFS was approximately 1.3 times in topsoil and 1.8 times in subsoil higher than those of CFS. Lower ratios of cy17:0 to $16:1{\omega}7c$ and cy19:0 to $18:1{\omega}7c$ were found in the CFS soils than the OFS soils, indicating that microbial stress decreased. The ratio of MUFA to SFA was higher in OFS due to organic input to the soil. In principal components analysis (PCA), the first variable accounted for 54.3%, while the second for 27.3%, respectively. The PC1 of the PCA separated the samples from CFS and OFS, while the PC2 of the PCA separated the samples from topsoil and subsoil. EL-FAMEs with the positive eigenvector coefficients for PC1 were cy17: 0 to $16:1{\omega}7c$ ratio, cy19:0 to $18:1{\omega}7c$ ratio, soil pH, soil organic matter, and soil $NO_3$-N content. Our findings suggest that the shifting cy19:0 to $18:1{\omega}7c$ ratio should be considered as potential factors responsible for the clear microbial community differentiation observed between different cultivation systems and soil depth in controlled horticultural land.

손바닥 선인장 열매 및 줄기 추출물의 생리활성(I)-일반약리검색 (Screening on Biological Activities of the Extracts from Fruit and Stem of Prickly Pear(Opuntia ficus-indica var. saboten))

  • 이정규;이영철;문영인;박희준;한용남;최종원
    • 생약학회지
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    • 제32권4호통권127호
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    • pp.330-337
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    • 2001
  • Prickly pear(Opuntia ficus-indica var. saboten Makino, Cactaceae) is a tropical or subtropical plant, which is widely used as folk medicine for burned wound, edema and indigestion. Screening on the biological properties of the fruits(OFS-Fr) and stems(OFS-St) of the plant was carried out to prove the pharmacological significance. By hot plate and acetic acid-inducing writhing methods, significant analgesic effects of OFS-Fr and OFS-St were found in mice and anti-edemic effect was observed in carrageenin-induced inflammatory rats. However, the extracts showed no significant actions on central and autonomic nervous system and blood circulatory system, which imply no toxic effects to animal.

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신 장애물제한표면에 관한 이론적 고찰과 실증분석 - 인천국제공항을 중심으로 - (A Theoretical Study and Empirical Analysis of New Obstacle Limitation Surface (OLS) - The Case of Incheon International Airport -)

  • 최상일;유수정;곽기열;김현미;김휘양
    • 한국항공운항학회지
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    • 제30권3호
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    • pp.28-37
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    • 2022
  • Obstacle Limitation Surface (OLS) is conceptual surface establishing the airspace around aerodromes to be maintained from obstacles to ensure safe aircraft operations. Despite advances in the technologies for aircraft, navigation systems and the development of new flight procedures, the criteria defining OLS have not been amended since its initial establishment, resulting in the overestimation of areas for height restriction. As there were requests to examine OLS at the 12th Air Navigation Conference and the 38th ICAO Assembly, the research on the OLS revision began in earnest and ICAO has proposed Obstacle Free Surface (OFS) and Obstacle Evaluation Surface (OES) as an alternative of the existing OLS. OFS is surfaces where obstacles shall not be permitted, and OES is ones where obstacles be evaluated with an aeronautical study and could be permitted under some conditions. The purpose of this study is to preemptively assess the efficiency and safety of OFS and OES by applying them to the second runway (15L/33R) of Incheon International Airport. The results show that OFS and OES are capable of serving the instrument flight procedure safely with a smaller obstacle clearance area compared to the existing OLS.

수중에서 2개의 광섬유센서를 이용한 초음파 음원의 위치 검출에 관한 연구 (A Study on Ultrasonic Location in Underwater Using the Two Optical Fiber Sensors)

  • 권태호;신대용;이준호;이종길
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2002년도 추계학술대회 논문집 Vol.15
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    • pp.62-66
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    • 2002
  • The preliminary results on the application of optical fiber sensor(OFS) for locations of ultrasonic signals in underwater are presented and analyzed. The OFS utilizing the principle of Sagnac interferometry was designed and the hollowed cylindrical mandrel wound by single mode optical fiber was used as sensing component. The ultrasonic signal source was simulated by the PZT actuator operated with function generator. It has been shown that the OFS could detect the signals less affected by ultrasonic path comparing to conventional acousto-electric sensor and accurate location of ultrasonic signal could be carried out using two OFSs.

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광섬유 센서를 이용한 절연유내의 부분방전 위치검출에 관한 연구 (A Study on Partial Discharge Location in Insulating Oil using Optical Fiber Sensors)

  • 권태호;이종길;이준호
    • 한국전기전자재료학회논문지
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    • 제16권9호
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    • pp.838-841
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    • 2003
  • This paper shows results on the application of optical fiber sensors(OFS) for locations of ultrasonic signals in silicon insulating oil. The OFS system based on the principle of Sagnac interferometry has been designed and established for this work. The hollowed cylindrical mandrel wound by single mode optical fiber was used as a sensing component and ultrasonic signals which simulate the partial discharge In the oil have been generated by PZT actuator operated with function generator. The experimental results shows that the OFS has a excellent performance for the PD location with resolutions less than 1$^{\circ}C$ error range in the miniature insulating oil tank.

손바닥선인장 추출물의 플라보노이드 구조 규명 및 HPLC-PDA를 이용한 지표성분의 함량 분석 (Identification of Flavonoids from Extracts of Opuntia ficus-indica var. saboten and Content Determination of Marker Components Using HPLC-PDA)

  • 박승배;강동현;진창배;김형자
    • 한국식품영양과학회지
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    • 제46권2호
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    • pp.210-219
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    • 2017
  • 손바닥선인장으로부터 기능성식품 개발을 위하여 최적의 에탄올 추출물 탐색과 HPLC-PDA 분석방법에 의한 validation을 실시하였다. 지표성분으로 dihydrokaempferol (DHK)과 3-O-methylquercetin(3-MeQ)을 선정하여 표준화를 실시하였으며 검출법 확립을 위한 정량분석은 Luna RP-18 칼럼($4.6{\times}250mm$, $5{\mu}m$)을 이용하여 1% 인산용액과 아세토니트릴을 전개 용매로 사용하였다. 용출은 1.0 mL/min의 유속으로 기울기 용출(gradient elution) 방법을 이용하였으며, 280 nm 파장에서는 DHK를, 360 nm 파장에서는 3-MeQ를 검출한 피크 면적을 이용하여 검량곡선을 작성하여 분석하였다. 본 연구에서 확립한 분석법으로 특이성, 직선성, 정밀성, 정확성, 회수율을 검색하였다. DHK의 검량선으로부터 상관계수($R^2$) 0.9998의 우수한 직선성과 intra-day와 inter-day 분석에서 97% 이상의 회수율과 3% 미만의 RSD를 나타내 정밀성과 정확성을 입증하였다. 3-MeQ의 검량선으로부터 상관계수($R^2$) 1의 우수한 직선성과 intra-day와 inter-day 분석에서 95% 이상의 회수율과 7% 미만의 RSD를 나타내 정밀성과 정확성을 입증하였다. DHK의 검출한계는 $1.38{\mu}g/mL$, 정량한계는 $4.18{\mu}g/mL$로 나타났으며, 3-MeQ의 검출한계는 $3.49{\mu}g/mL$, 정량한계는 $10.6{\mu}g/mL$로 나타났다. 손바닥선인장 에탄올 추출물(OFSEs)은 70과 $80^{\circ}C$에서 50, 70, 80% 에탄올로 3, 4, 5, 6시간 동안 각각 추출하였으며, 지표물질의 검량곡선을 활용하여 각각의 OFSEs로부터 두 종의 지표물질 함량을 분석하였다. 본 시험법으로 분석한 지표물질의 함량은 $80^{\circ}C$에서 추출한 70% OFSE가 DHK $26.42{\pm}0.65mg/OFS100g$, 3-MeQ $3.88{\pm}0.29mg/OFS100g$의 함량을 나타내 가장 우수하게 나타났다. 다양한 OFSEs에 대하여 DPPH 자유 라디칼 소거효능과 쥐의 간 균질액을 이용한 지질과산화 저해 효능에 대한 항산화 효능은 지표물질의 함량이 가장 높은 70% OFSE에서 우수한 효능을 나타내 본 연구에서 확립한 원료 표준화를 위한 적합한 분석법임이 검증되었다. 따라서 본 연구를 통하여 HPLC-PDA를 이용한 손바닥선인장 에탄올 추출물의 DHK와 3-MeQ의 분석법은 개별인정형 건강 기능식품 기능성 원료 개발을 위한 유용한 자료로 활용될 것으로 생각한다.