• 제목/요약/키워드: OCM

검색결과 57건 처리시간 0.023초

송이버섯과 동충하초 균사체를 혼합 배양한 한방추출물의 발효에 의한 생리활성 (Physiological Activity of Extracts from Mixed Culture of Medical Herbs and Mycelia of Tricholoma matsutake and Cordyceps militaris by Fermentation)

  • 이형범;김혜자;정명수;조화은;최윤희;임규상;이기남
    • 대한본초학회지
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    • 제23권1호
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    • pp.1-8
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    • 2008
  • Objectives : The purpose of this study was to investigate extracts from mixed culture of Oriental medicines and cereal medium and mycelia of Trichloma matsutake and Cordyceps militaris by fermentation to develop new material for pharmaceutical products and medicinal food, Methods : To evaluate physiological activities of OCM extracts, we examined antioxidant activity(total polyphenol contents, electronic donating ability, SOD-like activity), ${\beta}$-glucan contents, nitric oxide production and cytotoxicity by MTT assay. Results : Total polyphenol contents of fermented OCM(UF) and non-fermented OCM(UM) extracts were more than 40% UM and UF of DPPH radical scavenging activity was 25.67%, 23.43% respectively. Total polyphenol content of non-fermented extract (UME) was 12.57%, while that of fermented extract(UFE) was 7.05%. SOD like activity showed UM 85.35%, UF 76.18%, UME 58.42%, UFE 72.21%. UME, and UFE 31.43%, ${\beta}$-glucan contents of UME and UFE were more than 40%. NO productions of UME, and UFE showed a LPS dose dependent tendency. Cytotoxicity on Raw 264.7 cell showed more than 90% viability. Inhibitory effect of UFE on HT1080 cell growth was higher than UME. Conclusions : These results showed that extracts from mixed culture of mushroom mycelium and OCM have physiological activities which can be used in pharmaceutical products and medicinal food.

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Exploration of Functional Materials from Oriental Medicine Extracts Cultured with Tricholoma Matsutake Mycelium - (1) Physioactivity of Extracts in Accord with Extraction Methods -

  • Kim, Hae-Ja;Kim, Ki-Chul;Choi, Yun-Hee;Cho, Hwa-Eun;Hong, Hak-Gi;Han, Jung-Ho;Lee, Ki-Nam
    • 대한예방한의학회지
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    • 제12권2호
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    • pp.1-12
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    • 2008
  • The purpose of this study was to investigate extract from mixed culture with Trichloloma matsutake mycelium in oriental medicine and cereal medium(OCM) to develop new material for pharmaceutical products and medicinal food. To evaluate of physiological activity of OCM extracts, we examined ${\beta}$-glucan contents, SOD-like activity, nitric oxide production and cytotoxicity by MTT assay. ${\beta}$-glucan contents was found to be 52.85% for crude polysaccharide of hot water extracts of OCM(HEE) and 49.74% for crude polysaccharide of ultra sonic waves, micro waves, and micro bubble extracts of OCM (UEE). SOD like activity was showed UE 74.66%, HE 67.16%, UEE 31.34%, HEE 26.10%, respectively. NO production of UEE and HEE, at LPS 1 ug/mL, 1 mg/mL UEE showed 66.62 uM and HEE 45.68 uM. at LPS 10 ug/mL, 1 mg/mL UEE showed 63.91 uM and HEE 51.74 uM. The inhibitory effect against HT1080 was increased dose-dependently in UEE.

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Design Optimization of an Ozone Contactor Using Ozone Contactor Model (OCM) Software

  • Kim, Doo-Il;Lee, Chae-Young;Joe, Woo-Hyeun;Lee, Seock-Heon
    • Environmental Engineering Research
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    • 제14권4호
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    • pp.244-249
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    • 2009
  • Designing an ozone contactor is complicated because the residual ozone, log C. parvum inactivation, and bromate formation should be optimized with fluctuating water quality. OCM software was developed to assist a plant designer or an operator to fulfill the sophisticated optimization required in the design or operation of a new or an existing plant. In this article, numerical simulations were carried out using the OCM software for the design of a new ozone contactor under diverse design factors (i.e., three pHs, three temperatures, low and high dispersion numbers, and four and ten cells with complete mixing) with kinetic parameters obtained from the sand-filter effluent of a water treatment plant treating water from the Paldang impoundment. The results of the simulation suggested that a high residual ozone concentration at low pH and low temperature would be challenging, and PFR-like hydrodynamics could lower the residual ozone concentration. The inactivation of C. parvum oocysts increased at a lower pH. A lower dispersion number and more cell division increased the inactivation efficiency. Bromate was instantaneously formed during the initial ozonation stage. The effluent concentration was much lower than the regulatory levels imposed by the USEPA because of the low bromide level in raw water.

유분검출기 개발을 위한 빌지 배출수의 광특성 분석 (Optical Characteristic Analysis of Bilge Water for Developing an Oil Content Meter)

  • 최상화;황정웅;정병건
    • Journal of Advanced Marine Engineering and Technology
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    • 제25권2호
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    • pp.311-320
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    • 2001
  • Since 1998 for protection of marine pollution, all ships must have oil filtering equipment and 15ppm bilge alarms which satisfy Requirements of MARPOL 73/78. Oily-water separator used in machinery area of ships usually consists of two parts; one is filtering equipment and the other is oil content meter(OCM). This study presents optical characteristics of bilge were acquired form oil content sensing module. The oil content sensing module consists of IR-LED light source, photo-diode light receivers, and a glass tube for bilge water sample. The experiment with the bilge water demonstrates various valuable optical properties. These optical properties suggest notes and guides to make the low-cost, easy operation and good performance commercial type OCM that satisfy the requirements of MARPOL 73/78.

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운영연속성관리(OCM)관점에서 위기관리통합시스템 구축 (Comprehensive Crisis Management System of Operational Continuity Management)

  • 강희조
    • 한국항행학회논문지
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    • 제14권1호
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    • pp.127-133
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    • 2010
  • 운영연속성관리계획 수립을 위한 프로세스는 비즈니스 위험 평가, 취약성 분석, 업무영향분석, 업무복구전략수립, 상세계획수립, 계획실행, 테스트 및 유지관리(모니터링 포함)의 반복적인 사이클을 구성할 수 있다. 관리체계는 조직에 발생하는 재난 관리 체계를 개발, 수행, 개선, 유지와 업무 연속성 확보 체계를 위한 책임 의무사항 제공과 함께 재난예방, 재난대비, 재난대응, 재난복구를 위한 재난관리체계를 평가할 수 있다. 따라서 본 논문에서는 글로벌 환경변화에 대응하고 사회 안전 시스템의 구축과 운영의 극대화를 위하여 운영연속성관리, 위기관리체제 구축 및 위기관리통합체제 구축에 대하여 전반적으로 기술하고 조직 내에서 운영연속성관리를 이해하고 발전시켜 실행하기 위한 기틀을 제공하고자 운영연속성관리 관점에서의 위기관리통합시스템 구축 방법을 제안하였다.

디아자트리사이클로디온의 X선 결정구조 결정 (X-Ray Crystal Structure Determination of Diazatricyclodione)

  • 김상수;안종일;한보섭
    • 한국결정학회지
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    • 제1권2호
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    • pp.84-90
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    • 1990
  • 1,3-Diazatricyclo (5.2.1.O5.10) decade-2,4dione, C8H10N2O2의 단위세포 상수는a=6.585(7), b=9.089(4), c=12.937(10)A, β=95.72(5)˚, V=770.43, Dc=1.4391c0, 1=1.Ocm-1,7 =295 ˚K, 공간군은 P21/n이고 단사 정계이며 Z =4이 다. λ(Mo K u ) =0.7093A을 사용한 698개 의 회절 반점에 대한 최종 신뢰도 R값은 0.037이다. 본 화합물은 N1-(w-butenyl)uracil의 분자내 (2+2) 광고리화 반응 생성물이며, (5.1. 2.O5.10) tricyclic 계에 속한다. Inversion symmetry에 의해 관련된 한 쌍의 분자들은 uracil 부분의 02와H3간 의 강한 수소 결합을 하고있다

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디지털 필터뱅크 기반 플렉서블 위성중계기를 위한 채널화 기법의 성능비교 연구 (Performance Comparison of Channelization Schemes for Flexible Satellite Transponder with Digital Filter Banks)

  • 이동훈;김기선
    • 한국군사과학기술학회지
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    • 제13권3호
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    • pp.405-412
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    • 2010
  • The purpose of this paper is to compare complexity and to assess flexibility of competing transponder architectures for satellite communication services. For performance comparison, we consider three channelization techniques: digital down converter(DDC) based on the use of the cascaded integrator-comb(CIC) filter, tuneable pipeline frequency transform(T-PFT) based on the tree-structure(TS) and variable oversampled complex-modulated filter banks(OCM-FB) based on the polyphase FFT(P-FFT). The comparison begins by presenting a basic architecture of each channelization method and includes analytical expressions of the number of multiplications as a computational complexity perspective. The analytical results show that DDC with CIC filter requires the heavy computational burden and the perfect flexibility. T-PFT based on the TS provides the almost perfect flexibility with the low complexity over DDC with the CIC filter for a large number of sub-channels. OCM-FB based on the P-FFT shows the high flexibility and the best computational complexity performance compared with other approaches.

해수색 원격탐사에 의한 동해 연근해역 클로로필 a 이상분포 연구 (Study on Abnormal Distribution of High Concentration Chlorophyll a in the East Sea of Korea in Spring Season using Ocean Color Satellite Remote Sensing)

  • 서영상
    • 한국환경과학회지
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    • 제15권1호
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    • pp.59-66
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    • 2006
  • High concentration of chlorophyll a occurred around the Ulleung Warm Eddy off Ulleung Island in the East Sea of Korea in spring season. The abnormal distributions of chlorophyll a were captured by satellite remote sensing and measured field data. The temporal and spatial scale of the abnormal distributions were around 20days and 50km diameter off Ullung Island. The anomalies were quantified b)'estimated chlorophyll a derived from OCM and SeaWiFS ocean color data from 2000 to 2004. The origin of abnormal hish concentrations was estimated by this study. It was that suspended material discharged from the Nakdong River and the coastal water located in the southeastern part of Korean Peninsula moved to northeastern coast, and then moved to off Ullung island, The high chlorophyll a concentrations including inorganic materials were accumulated by anticyclonic eddy such as the Ullung Warm Eddy around Ullung island in the East Sea of Korea in spring season.

금속 산화물 촉매를 이용한 메탄의 Oxidative Coupling 반응 (Oxidative Coupling of Methane by Metal Oxide Catalysts)

  • 김형진;편무실;박홍수;함현식
    • 공업화학
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    • 제4권4호
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    • pp.807-813
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    • 1993
  • 메탄의 직접 전환 기술 중 하나인 OCM(oxidative coupling of methane) 반응을 수행하였다. 사용한 금속 산화물 촉매는 Li/MgO와 Pb/MgO이었다. 온도에 따른 촉매의 반응성을 알아보기 위해 600, 700, $800^{\circ}C$에서 반응을 행하였으며, 반응물 혼합비(메탄:산소)에 따른 반응성, 전환율 및 선택도를 알아보기 위해 $700^{\circ}C$에서 혼합비를 2:1 및 1:1로 하여 실험을 하였다. 그 결과 7wt% Li/MgO 촉매의 경우 반응온도 $700^{\circ}C$이고 혼합비가 2:1일 때 메탄의 전환율과 $C_2$화합물의 선택도가 각각 20%, 65% 정도로서 우수한 반응성을 보임을 알 수 있었다. 7wt% Li/MgO 촉매의 경우 $700^{\circ}C$에서 혼합비가 1:1일 때 메탄의 전환율은 30%로 증가했으나 $C_2$화합물의 선택도는 45%로 감소하였다. Pb/MgO 촉매는 Li/MgO 보다 낮은 선택도를(25%) 나타내었다.

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Comamonas sp. Strain DJ-12로부터 Protocatechuate의 분해에 관여하는 pmcABCDEFT 유전자군의 구조 분석 (Structure Analysis of pmcABCDEFT Gene Cluster for Degradation of Protocatechuate from Comamonas sp. Strain DJ-12)

  • 강철희;이상만;이경;이동훈;김치경
    • 미생물학회지
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    • 제41권3호
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    • pp.195-200
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    • 2005
  • Comamonas sp. strain DJ-12의 pmcABCDEFT 유전자군은 protocatechuate (PCA)의 분해과정에 관여하는 PCA 4,5-dioxygenase, 4-carboxy-2hydroxymuconic semialdehyde (CHMS) dehydrogenase, 2-pyrone04,5-dicarboxylate(PDC) hydrolase, 4-oxalomesaconate (OMA) hydratase, 그리고 4-oxalocitramalate (OCM) aldolase 등의 효소들을 생산하는 유전자들과 transporter의 역학을 하는 유전자로 각각 확인되었다. 이 유전자군은 Comamonas sp. strain DJ-12의 chromosomal DNA로부터 얻은 PCR 산물들을 T-vector에 ligation하여 재조합 플라스미드 pMT1, pMT2, pMT3, pMT4, pMT5, pMT6, pMT7, pMT8, pMT9, pMT10을 제조하였다. 이들 재조합 플라스미드의 염기서열을 분석한 결과 PCA 4,5-dioxygenase 유전자는 alpha(pmcA)와 beta(pmcB) 두 개의 subunit으로 구성 되어있으며, 각각 450 bp와 870 bp이었다. CHMS dehydrogenase 유전자(pmcC)는 960 bp, PDC hydrolase 유전자(pmcD)는 918 bp이였으며, OMA hydratase 유전자(pmcE)는 1029 bp, OCM aldolase 유전자 (pmcF)는 689 bp, 그리고 transporter 유전자(pmcT)는 1,398 bp이였다. 이들 pmc 유전자들은 pmcT-pmcE-pmcF-pmcD-pmcA-pmcB-pmcC의 순서로 배열되어 있었다. Comamonas sp. strain DJ-12의 pmcABCDEFT 유전자산물의 아미노산 서열을 분석한 결과, Comamonas testosteroni BR6020 및 Psedomonas ochraceae NG.J1와 $94{\~}98\%$의 높은 유사성을 보였고, 그 유전자들의 배열 순서도 동일하였다. 그러나 Sphingomonas paucimobilis SYK-6, Sphingomonas sp. LB126, 그리고 Arthrobacter keyser 12B와는 아미노산 서열이 $52{\~}74\%$의 유사성을 보였고, 그 유전자의 배열 구조도 상이하였다.