• Title/Summary/Keyword: OCM

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Physiological Activity of Extracts from Mixed Culture of Medical Herbs and Mycelia of Tricholoma matsutake and Cordyceps militaris by Fermentation (송이버섯과 동충하초 균사체를 혼합 배양한 한방추출물의 발효에 의한 생리활성)

  • Lee, Hyoung-Beom;Kim, Hae-Ja;Chong, Myong-Soo;Cho, Hwa-Eun;Choi, Yun-Hee;Lim, Kyu-Sang;Lee, Ki-Nam
    • The Korea Journal of Herbology
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    • v.23 no.1
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    • pp.1-8
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    • 2008
  • Objectives : The purpose of this study was to investigate extracts from mixed culture of Oriental medicines and cereal medium and mycelia of Trichloma matsutake and Cordyceps militaris by fermentation to develop new material for pharmaceutical products and medicinal food, Methods : To evaluate physiological activities of OCM extracts, we examined antioxidant activity(total polyphenol contents, electronic donating ability, SOD-like activity), ${\beta}$-glucan contents, nitric oxide production and cytotoxicity by MTT assay. Results : Total polyphenol contents of fermented OCM(UF) and non-fermented OCM(UM) extracts were more than 40% UM and UF of DPPH radical scavenging activity was 25.67%, 23.43% respectively. Total polyphenol content of non-fermented extract (UME) was 12.57%, while that of fermented extract(UFE) was 7.05%. SOD like activity showed UM 85.35%, UF 76.18%, UME 58.42%, UFE 72.21%. UME, and UFE 31.43%, ${\beta}$-glucan contents of UME and UFE were more than 40%. NO productions of UME, and UFE showed a LPS dose dependent tendency. Cytotoxicity on Raw 264.7 cell showed more than 90% viability. Inhibitory effect of UFE on HT1080 cell growth was higher than UME. Conclusions : These results showed that extracts from mixed culture of mushroom mycelium and OCM have physiological activities which can be used in pharmaceutical products and medicinal food.

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Exploration of Functional Materials from Oriental Medicine Extracts Cultured with Tricholoma Matsutake Mycelium - (1) Physioactivity of Extracts in Accord with Extraction Methods -

  • Kim, Hae-Ja;Kim, Ki-Chul;Choi, Yun-Hee;Cho, Hwa-Eun;Hong, Hak-Gi;Han, Jung-Ho;Lee, Ki-Nam
    • Journal of Society of Preventive Korean Medicine
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    • v.12 no.2
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    • pp.1-12
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    • 2008
  • The purpose of this study was to investigate extract from mixed culture with Trichloloma matsutake mycelium in oriental medicine and cereal medium(OCM) to develop new material for pharmaceutical products and medicinal food. To evaluate of physiological activity of OCM extracts, we examined ${\beta}$-glucan contents, SOD-like activity, nitric oxide production and cytotoxicity by MTT assay. ${\beta}$-glucan contents was found to be 52.85% for crude polysaccharide of hot water extracts of OCM(HEE) and 49.74% for crude polysaccharide of ultra sonic waves, micro waves, and micro bubble extracts of OCM (UEE). SOD like activity was showed UE 74.66%, HE 67.16%, UEE 31.34%, HEE 26.10%, respectively. NO production of UEE and HEE, at LPS 1 ug/mL, 1 mg/mL UEE showed 66.62 uM and HEE 45.68 uM. at LPS 10 ug/mL, 1 mg/mL UEE showed 63.91 uM and HEE 51.74 uM. The inhibitory effect against HT1080 was increased dose-dependently in UEE.

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Design Optimization of an Ozone Contactor Using Ozone Contactor Model (OCM) Software

  • Kim, Doo-Il;Lee, Chae-Young;Joe, Woo-Hyeun;Lee, Seock-Heon
    • Environmental Engineering Research
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    • v.14 no.4
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    • pp.244-249
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    • 2009
  • Designing an ozone contactor is complicated because the residual ozone, log C. parvum inactivation, and bromate formation should be optimized with fluctuating water quality. OCM software was developed to assist a plant designer or an operator to fulfill the sophisticated optimization required in the design or operation of a new or an existing plant. In this article, numerical simulations were carried out using the OCM software for the design of a new ozone contactor under diverse design factors (i.e., three pHs, three temperatures, low and high dispersion numbers, and four and ten cells with complete mixing) with kinetic parameters obtained from the sand-filter effluent of a water treatment plant treating water from the Paldang impoundment. The results of the simulation suggested that a high residual ozone concentration at low pH and low temperature would be challenging, and PFR-like hydrodynamics could lower the residual ozone concentration. The inactivation of C. parvum oocysts increased at a lower pH. A lower dispersion number and more cell division increased the inactivation efficiency. Bromate was instantaneously formed during the initial ozonation stage. The effluent concentration was much lower than the regulatory levels imposed by the USEPA because of the low bromide level in raw water.

Optical Characteristic Analysis of Bilge Water for Developing an Oil Content Meter (유분검출기 개발을 위한 빌지 배출수의 광특성 분석)

  • 최상화;황정웅;정병건
    • Journal of Advanced Marine Engineering and Technology
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    • v.25 no.2
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    • pp.311-320
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    • 2001
  • Since 1998 for protection of marine pollution, all ships must have oil filtering equipment and 15ppm bilge alarms which satisfy Requirements of MARPOL 73/78. Oily-water separator used in machinery area of ships usually consists of two parts; one is filtering equipment and the other is oil content meter(OCM). This study presents optical characteristics of bilge were acquired form oil content sensing module. The oil content sensing module consists of IR-LED light source, photo-diode light receivers, and a glass tube for bilge water sample. The experiment with the bilge water demonstrates various valuable optical properties. These optical properties suggest notes and guides to make the low-cost, easy operation and good performance commercial type OCM that satisfy the requirements of MARPOL 73/78.

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Comprehensive Crisis Management System of Operational Continuity Management (운영연속성관리(OCM)관점에서 위기관리통합시스템 구축)

  • Kang, Heau-Jo
    • Journal of Advanced Navigation Technology
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    • v.14 no.1
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    • pp.127-133
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    • 2010
  • The process for establishment of Operational Continuity Management Plan is organized repeatedly of Business Risk Assessment, Crisis Analysis, Business Impact Analysis, Establishing Business Recovery Strategies, Detailed Planning, Plan Execution, Test and Maintenance(Including Monitoring). Therefore, in this paper in response to global environmental change and the construction and operation of social security systems to maximize operational continuity management, crisis management and crisis management systems, building integrated systems for building technology in general and operational continuity management within an organization to understand developed to provide a framework for implementing operational continuity management in terms of crisis management has proposed to build an integrated system.

X-Ray Crystal Structure Determination of Diazatricyclodione (디아자트리사이클로디온의 X선 결정구조 결정)

  • Kim, Sang-Su;An, Jong-Il;Han, Bo-Seop
    • Korean Journal of Crystallography
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    • v.1 no.2
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    • pp.84-90
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    • 1990
  • 1,3-Diazatricyclo (5.2.1.Os .to) decane-2,11-dione , C8H10N2O2. MW=166.15, Monoclinic, P2/n a= 6.585(7) , b=9.089(4), c=12.937(10)A, β=95.72(5)˚, V=770.4A3, Z=4, Dc=1.43(4) g/cm3, λ (Mo Ka)=0.7093A, r=1.Ocm-1, T=295˚K, final R=0.037 for 698 unique observed reflections. The compound is the product of the intramolecular (2+2) photocycloaddition of Nl-(w-butenyl)uracil and belongs to a (5.2.1.O5.10) tricyclic system. A pair of molecules related by the inversion symmetry are held together by the strong hydrogen bonding interactions between 02 and H3 of the uracil moiety.

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Performance Comparison of Channelization Schemes for Flexible Satellite Transponder with Digital Filter Banks (디지털 필터뱅크 기반 플렉서블 위성중계기를 위한 채널화 기법의 성능비교 연구)

  • Lee, Dong-Hun;Kim, Ki-Seon
    • Journal of the Korea Institute of Military Science and Technology
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    • v.13 no.3
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    • pp.405-412
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    • 2010
  • The purpose of this paper is to compare complexity and to assess flexibility of competing transponder architectures for satellite communication services. For performance comparison, we consider three channelization techniques: digital down converter(DDC) based on the use of the cascaded integrator-comb(CIC) filter, tuneable pipeline frequency transform(T-PFT) based on the tree-structure(TS) and variable oversampled complex-modulated filter banks(OCM-FB) based on the polyphase FFT(P-FFT). The comparison begins by presenting a basic architecture of each channelization method and includes analytical expressions of the number of multiplications as a computational complexity perspective. The analytical results show that DDC with CIC filter requires the heavy computational burden and the perfect flexibility. T-PFT based on the TS provides the almost perfect flexibility with the low complexity over DDC with the CIC filter for a large number of sub-channels. OCM-FB based on the P-FFT shows the high flexibility and the best computational complexity performance compared with other approaches.

Study on Abnormal Distribution of High Concentration Chlorophyll a in the East Sea of Korea in Spring Season using Ocean Color Satellite Remote Sensing (해수색 원격탐사에 의한 동해 연근해역 클로로필 a 이상분포 연구)

  • Suh Young-Sang
    • Journal of Environmental Science International
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    • v.15 no.1
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    • pp.59-66
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    • 2006
  • High concentration of chlorophyll a occurred around the Ulleung Warm Eddy off Ulleung Island in the East Sea of Korea in spring season. The abnormal distributions of chlorophyll a were captured by satellite remote sensing and measured field data. The temporal and spatial scale of the abnormal distributions were around 20days and 50km diameter off Ullung Island. The anomalies were quantified b)'estimated chlorophyll a derived from OCM and SeaWiFS ocean color data from 2000 to 2004. The origin of abnormal hish concentrations was estimated by this study. It was that suspended material discharged from the Nakdong River and the coastal water located in the southeastern part of Korean Peninsula moved to northeastern coast, and then moved to off Ullung island, The high chlorophyll a concentrations including inorganic materials were accumulated by anticyclonic eddy such as the Ullung Warm Eddy around Ullung island in the East Sea of Korea in spring season.

Oxidative Coupling of Methane by Metal Oxide Catalysts (금속 산화물 촉매를 이용한 메탄의 Oxidative Coupling 반응)

  • Kim, Hyung-Jin;Pyun, Moo-Sil;Park, Hong-Soo;Hahm, Hyun-Sik
    • Applied Chemistry for Engineering
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    • v.4 no.4
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    • pp.807-813
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    • 1993
  • Oxidative coupling of methane(OCM), one of the methods of direct methane conversion, was performed. Metal oxide catalysts used were Li/MgO and Pb/MgO. To investigate the reactivity of the catalysts with temperature, the reaction was carried out at 600, 700 and $800^{\circ}C$; and to investigate the effect of the feed ratio of the reactants($CH_4:O_2$) on reactivity, conversion, and selectivity the reaction was performed at $700^{\circ}C$ with the feed ratio of 2:1 and 1:1. The results indicate that 7wt% Li/MgO catalyst is a good catalyst for OCM reaction with 20% conversion and 65% selectivity at $700^{\circ}C$ with the feed ratio of 2:1. As feed ratio was 1:1, methane conversion was increased to 30% while $C_2$ selectivity decreased to 45% at $700^{\circ}C$ with 7wt% Li/MgO catalyst. The Pb/MgO catalyst showed less selectivity(25%) than Li/MgO did.

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Structure Analysis of pmcABCDEFT Gene Cluster for Degradation of Protocatechuate from Comamonas sp. Strain DJ-12 (Comamonas sp. Strain DJ-12로부터 Protocatechuate의 분해에 관여하는 pmcABCDEFT 유전자군의 구조 분석)

  • Kang Cheol-Hee;Lee Sang-Mhan;Lee Kyoung;Lee Dong-Hun;Kim Chi-Kyung
    • Korean Journal of Microbiology
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    • v.41 no.3
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    • pp.195-200
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    • 2005
  • Comamonas sp. strain DJ-12 is a bacterial isolate capable of degrading of 4-chlorobiphenyl (4CB) as a carbon and energy source. The degradation pathway was characterized as being conducted by consecutive reactions of the meta-degradation of 4CB, hydrolytic dechlorination of 4-chlorobenzoate (4CBA), hydroxylation of 4-hydroxybenzoate, and meta-degradation of protocatechuate to product TCA metabolites. The 6.8 kb fragment from the chromosomal DNA of Comamonas sp. strain DJ-12 included the genes encoding for the meta-degradation of PCA; the genes of protocatechuate 4,5-dioxygenase alpha and beta subunits (pmcA and pmcB), 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (pmcC), 2-pyrone-4,6-dicarboxylate hydrolase (pmcD), 4-oxalomesaconate (OMA) hydratase(pmcE), 4-oxalocitramalate (OCM) aldolase (pmcF), and transporter gene (pmcT). They were organized in the order of pmcT-pmcE-pmcF-pmcD-pmcA-pmcB-pmcC. The amino acid sequences deduced from the nucleotide sequences of pmcABCDEFT genes from Comamonas sp. strain DJ-12 exhibited 94 to $98\%$ homologies with those of Comamonas testosteroni BR6020 and Pseudomonas ochraceae NGJ1, but only 52 to $74\%$ with homologies Sphingomonas paucimobilis SYK-6, Sphingomonas sp. LB126, and Arthrobacter keyseri 12B.