• BMB Reports
• /
• 제52권2호
• /
• pp.133-138
• /
• 2019

Upon viral infection, the 2', 5'-oligoadenylate synthetase (OAS)-ribonuclease L (RNaseL) system works to cleave viral RNA, thereby blocking viral replication. However, it is unclear whether OAS proteins have a role in regulating gene expression. Here, we show that OAS1 and OAS3 act as negative regulators of the expression of chemokines and interferon-responsive genes in human macrophages. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein-9 nuclease (Cas9) technology was used to engineer human myeloid cell lines in which the OAS1 or OAS3 gene was deleted. Neither OAS1 nor OAS3 was exclusively responsible for the degradation of rRNA in macrophages stimulated with poly(I:C), a synthetic surrogate for viral double-stranded (ds)RNA. An mRNA sequencing analysis revealed that genes related to type I interferon signaling and chemokine activity were increased in $OAS1^{-/-}$ and $OAS3^{-/-}$ macrophages treated with intracellular poly(I:C). Indeed, retinoic-acid-inducible gene (RIG)-I- and interferon-induced helicase C domain-containing protein (IFIH1 or MDA5)-mediated induction of chemokines and interferon-stimulated genes was regulated by OAS3, but Toll-like receptor 3 (TLR3)- and TLR4-mediated induction of those genes was modulated by OAS1 in macrophages. However, stimulation of these cells with type I interferons had no effect on OAS1- or OAS3-mediated chemokine secretion. These data suggest that OAS1 and OAS3 negatively regulate the expression of chemokines and interferon-responsive genes in human macrophages.

• 한국축산시설환경학회지
• /
• 제14권1호
• /
• pp.1-8
• /
• 2008

This study was conducted to solve the problem of public grievance owing to odor of a pig farm. Odor emissions from pig production systems mainly originate from liquid manure storage and solid manure fermentation. The low-cost odor abatement system (OAS) for application at liquid manure storage tank and solid manure fermentation facilities was developed in this study. The OAS adapted odor removing principles of a biofilter and biotrickling filter. The OAS is very simplified in structure. The appearance of the OAS had a form of cylindrical or cubical shape. The system performance was monitored for about one year after stabilization. A 7 seconds empty bed contact time for the OAS was adapted to achieve the odor reduction levels. The commercial type of OAS was constructed with media comprised of wood chips. Moisture content always remained above 50% wet basis. Average ammonia removal efficiency for the developed design was 89% at the liquid manure storage tank. Also, the removal efficiency at a solid manure fermentation facility was 86% on ammonia.

• BMB Reports
• /
• 제29권3호
• /
• pp.210-214
• /
• 1996

The pH dependence of the kinetic parameters of mutant O-acetylserine sulfhydrylase (OASS) from Salmonella typhimurium LT-2 has been determined in order to obtain information on the chemical mechanism. The initial velocity pattern obtained by varying the concentrations of OAS at several fixed concentrations of TNB, shows an intersection on the left of the ordinate at pH 7.0, indicating that the kinetic mechanism is a sequential mechanism in which substrate inhibition by OAS is observed while the wild type enzyme showed a ping pong mechanism. The values of $V/E_t$, $V/K_{OAS}E_{t}$ and $V/K_{TNB}E_{t}$ decreased by about 68%, 14% and 16% as compared with the wild type enzyme. The $V/K_{OAS}E_{t}$ is a pK of 6.5 on the acid side of the pH profile, and the $V/K_{TNB}$ is pH independent. As compared with the wild type enzyme, the pKs in the V/K profiles are shifted, reflecting that binding of the cofactor in free E:OAS is less asymmetric.

• Journal of Microbiology and Biotechnology
• /
• 제9권3호
• /
• pp.235-242
• /
• 1999

We investigated the growth-inhibitory mechanism of Helicobacter pylori by omeprazole (OMP) and its activated sulfenamide (OAS). Using dithiothreitol (DTT) and 5,5'-dithio-bis[2-nitrobenzoic acid] (DTNB; Ellman's reagent), we first determined the relationship between the binding capacity of these compounds to H. pylori membrane and its significance to membrane P-type ATPase activity. After incubation of the intact H. pylori cells with either OMP or OAS, the residual quantity of free SH-groups on the cell membrane was measured, and, the resulting values were plotted as a function of time. From this experiment, we found that there was a considerable difference in the membrane-binding rates between OMP and OAS. At neutral pH, the disulfide bond formation on H. pylori membrane was completed within 2 min of incubation of the intact cells with OAS. By OMP, however, it was gradually formed, exceeding 10 min of incubation for completion, whereby, the extent of P-type ATPase inhibition appeared to be proportional to the disulfide forming rate. From this data, it was suggested that the disulfide formation might directly affect enzyme activity. Since OMP per se cannot yield a disulfide bond with cysteine, it is predicted that the enzyme inactivation must be caused by the OAS form. Accordingly, we postulated that, under the neutral pH, OMP could be converted to OAS in the course of transport. By extrapolating the inhibitory slopes, we could evaluate K₁ values, relating to their minimal inhibitory concentrations (MICs) for H. pylori growth. In these MIC ranges, H. pylori uptake or vesicular export of nutrients such as peptides were totally prohibited, but their effect in Escherichia coli were negligible. From these observations, we strongly suggest that the P-type ATPase activity is essential for the survival of H. pylori cells in particular.

• 한국지능시스템학회논문지
• /
• 제22권2호
• /
• pp.218-224
• /
• 2012

최근에는, 초소형 AUV(Autonomous Underwater Vehicle)의 개발에 대한 요구가 증가하고 있으므로 그 요소 기술의 확보가 시급하다. 요소 기술의 하나로서 국부경로제어의 기존 연구에서는 주로 전방감시소나(Forward Looking Sonar : FLS)의 정보를 활용하고 있는데, FLS의 크기는 초소형 AUV에 적합하지 않으므로 장애물회피소나(Obstacle Avoidance Sonar : OAS)를 이용하는 것이 바람직하다. 요약하면, 초소형 AUV를 위한 OAS 기반의 국부경로제어 시스템은 다음과 같은 문제점들을 가지고 있다. 즉, OAS는 낮은 방위(bearing) 분해능 및 지역적인 거리(range) 정보를 제공하며, 임무시간을 증대하기 위해서 에너지 소비가 적은 시스템을 필요로 한다. 나아가, 구조 및 파라메터 관점에서 용이한 설계 절차를 요구한다. 이 문제를 해결하기 위해서 OAS 빔 모델링을 기반으로 진화 전략(Evolution Strategy : ES) 및 퍼지논리 제어기(Fuzzy Logic Controller : FLC)를 이용하는 지능형 국부경로제어 기법이 제안되었다. 제안된 기법의 성능을 검증하고 특성을 분석하기 위해서 수중비행체(Underwater Flight Vehicle : UFV)의 수평면 침로(course) 제어가 수행되었다. 시뮬레이션 결과는 제안된 기법에 있어서 실제 적용의 가능성과 추가 연구의 필요성을 보여준다.

• 컴퓨터교육학회논문지
• /
• 제21권1호
• /
• pp.51-58
• /
• 2018

인공 신경망은 최근 다양한 분야에서 뛰어난 성능을 보여주고 있다. 하지만 인공 신경망이 학습한 지식이 정확히 어떤 내용인지를 사람이 파악하기 어렵다는 문제점이 존재하는데, 이를 해결하기 위한 방법 중 하나로 학습된 인공 신경망에서 규칙을 추출하는 방법들이 연구되고 있다. 본 연구에서는 학습된 인공 신경망으로부터 규칙을 추출하는 방법 중 하나인 ordered-attribute search(OAS) 알고리즘을 사용하여 인공 신경망으로부터 규칙을 추출해보고, 추출된 규칙을 개선하기 위해 규칙들을 분석하였다. 그 결과로 은닉 층의 출력값 분포가 OAS 알고리즘을 이용해 추출된 규칙의 정확도에 영향을 주는 것을 파악하였고, 은닉 유닛 명확화 기법을 통해 은닉 층 출력값을 이진화하여 효율적인 규칙을 추출할 수 있음을 제시하였다.

• 생명과학회지
• /
• 제19권10호
• /
• pp.1403-1409
• /
• 2009

본 연구에서는 중년비만여성을 대상으로 비만의 발생요인으로 예상되는 요인을 이용하여 비만의 상태를 연령 척도화하여 비만연령(Obesity Age: OA)이라는 연령 추정식을 산출함과 동시에 비만도(비만연령)를 실제의 역연령과 검토하였고 비만연령의 추정식은 다음과 같다. OAS (Obesity Age Score)=$0.106*X_1+0.035*X_2+0.048*X_3+0.041*X_4+0.003*X_5-0.037*X_6-10.66$ (1) ($X_1$: BMI, $X_2$: 체중, $X_3$: 체지방률, $X_4$: 배둘레, $X_5$: 중성지방, $X_6$: $VO_{2max}$) OA (Obesity Age)=7.3*OAS+49.6*(-1) (2) Z=(CA-49.6)(1-0.03) (3) OAc (Obesity Age corrected)=1.03*CA-7.3*OAS+1.47 (4) 그리고 역연령과 추정된 비만연령(Obesity Age corrected: OAc)의 비교에서는 유의한 차이를 나타내지 않아 타당성이 확인되었으며, 총 6개의 측정항목으로 구성된 비만연령을 통해 비만자 개개인의 비만건강도(상태)를 용이하게 평가할 수 있을 것으로 기대하며, 추후에는 다른 집단에서의 적용가능 여부를 알아보기 위해 교차타당성을 검토해야할 것이라고 사료된다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.36-36
• /
• 2017

Out of twenty common protein amino acids, there are many kinds of non protein amino acids (NPAAs) that exist as secondary metabolites and exert ecological functions in plants. Mimosine (Mim), one of those NPAAs derived from L. leucocephala acts as an iron chelator and reversely block mammalian cell cycle at G1/S phases. Cysteine (Cys) is decisive for protein and glutathione that acts as an indispensable sulfur grantor for methionine and many other sulfur-containing secondary products. Cys biosynthesis includes consecutive two steps using two enzymes-serine acetyl transferase (SAT) and O-acetylserine (thiol)lyase (OASTL) and appeared in plant cytosol, chloroplast, and mitochondria. In the first step, the acetylation of the ${\beta}$-hydroxyl of L-serine by acetyl-CoA in the existence of SAT and finally, OASTL triggers ${\alpha}$, ${\beta}$-elimination of acetate from OAS and bind $H_2S$ to catalyze the synthesis of Cys. Mimosine synthase, one of the isozymes of the OASTLs, is able to synthesize Mim with 3-hydroxy-4-pyridone (3H4P) instead of $H_2S$ for Cys in the last step. Thus, the aim of this study was to clone and characterize the cytosolic (Cy) OASTL gene from L. leucocephala, express the recombinant OASTL in Escherichia coli, purify it, do enzyme kinetic analysis, perform docking dynamics simulation analysis between the receptor and the ligands and compare its performance between Cys and Mim synthesis. Cy-OASTL was obtained through both directional degenerate primers corresponding to conserved amino acid region among plant Cys synthase family and the purified protein was 34.3KDa. After cleaving the GST-tag, Cy-OASTL was observed to form mimosine with 3H4P and OAS. The optimum Cys and Mim reaction pH and temperature were 7.5 and $40^{\circ}C$, and 8.0 and $35^{\circ}C$ respectively. Michaelis constant (Km) values of OAS from Cys were higher than the OAS from Mim. Inter fragment interaction energy (IFIE) of substrate OAS-Cy-OASTL complex model showed that Lys, Thr81, Thr77 and Gln150 demonstrated higher attraction force for Cys but 3H4P-mimosine synthase-OAS intermediate complex showed that Gly230, Tyr227, Ala231, Gly228 and Gly232 might provide higher attraction energy for the Mim. It may be concluded that Cy-OASTL demonstrates a dual role in biosynthesis both Cys and Mim and extending the knowledge on the biochemical regulatory mechanism of mimosine and cysteine.

• 대한한방내과학회지
• /
• 제26권1호
• /
• pp.74-92
• /
• 2005

Objectives/Methods : To analyze the effect of Injinchunggantang(IJCGT) to Interferon-${\alpha}/{\beta}$ signal transmission system in HepG2 cells, HepG2 Cell were treated with IJCGT. Also, revelation of MxA, 2'5'-OAS mRNA leaded by Interferon-${\alpha}/{\beta}$ and revelation and activation of Jak1, TYK1, and STAT 1, all main signal transmission factors, were analyzed. Results : The analysis resulted in the following 1. With interferon ${\alpha}/{\beta}$ there was no affect cell propagation of Hep G2 cells. With IJCGT alone, cell propagation of HepG2 was promoted, and cell propagation control function was recovered. 2. With interferon ${\alpha}/{\beta}$ cell death was unaffected. With IJCGT apoptosis of HepG2 cell was restrained, and the cell's reaction to interferon was unaffected. 3. With interferon ${\alpha}/{\beta}$ treatment mRNA revelation of MxA and 2'5'-OAS was induced. When HepG2 cells were injected with IJCGT without interferon ${\alpha}/{\beta}$ treatment, mRNA revelation of MxA and 2'5'-OAS increased in proportion to the treatment density. With pre-treatment of IJCGT, leaded with interferon ${\alpha}/{\beta}$, promoted revelation of MxA, 2'5' -OAS mRNA. 4. Though mRNA revelation of lakl, TYK1 and STAT1 was unaffected with IJCGT, activation of STAT1 was promoted with an increase of phosphorylation of STAT1 protein. With pre-treatment of IJCGT, Jak1, TYK2, STAT1 phosphorylation, leaded with interferon, strengthened. 5. TNF-a, IL-1b and LPS present, revelation of MxA and 2'5'-OAS mRNA leaded by interferon was restrained when HepG2 cells were treated with IJCGT, and the interferon signal transmission system restraint action leaded by inflammatory cytokines was moderated. Conclusion : These results support a role for IJGCT in promotion of anti-virus action through maintainance of the liver's sensibility toward interferon. A clinical study of an interferon treated patient treated also with IJGCT is needed to determine its efficacy.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2002년도 ITC-CSCC -2
• /
• pp.751-754
• /
• 2002

This paper introduces a way to realize biquadratic transfer functions using Operational Amplifiers (OAs) and Operational Transconductance Amplifiers (OTAs). The basic circuit configuration is constructed with two OAs and five OTAs. It is shown that low-pass, band-pass, high-pass, band-stop and all-pass transfer functions can be realized by suitably choosing the input terminals. And the circuit parameters can also be set by the transconductance gains of the OTAs independently. An example is given together with simulated results by PSPICE.