• 정보관리학회지
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• 제37권4호
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• pp.207-223
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• 2020

본 연구는 국내 문헌정보학 분야 연구자가 인용한 해외 학술지 논문의 OA 접근가능성을 살펴보고자 하였다. 이를 위해 정보관리학회지에 인용된 해외 학술지 논문 1,543편을 수집하였고 해외 학술지의 OA 정책과 OA 정책상 OA로 공개가 가능한 논문 현황을 조사하였다. 그리고 OA finder(Google Scholar, Unpaywall, OA Button)들을 활용하여 실제로 온라인에서 어느 정도까지 접근이 가능한지를 조사하였다. 조사결과, 해외 학술지들은 대부분 그린 OA 정책을 채택하고 있었다. 그리고 해외 학술지 논문의 95.4%에 해당하는 1,476편의 논문이 정책상 OA로 공개가 가능하였다. 이에 OA로 공개가 가능한 논문들에 대해 OA finder들을 활용하여 접근가능여부를 파악한 결과, Google Scholar가 68%로 가장 높은 OA 접근성을 보였고 Unpaywall과 OA Button은 각각 39%와 36%의 비슷한 OA 접근성을 보였다. 그리고 이들 OA finder들을 조합할 경우 최대 72%까지 OA 접근성이 상승하는 것을 확인하였다.

• The Plant Pathology Journal
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• 제38권4호
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• pp.287-295
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• 2022

Citrus tristeza virus (CTV) is efficiently transmitted in a semi-persistent manner by the brown citrus aphid (Toxoptera citricida (Kirkaldy)). Currently, the most sensitive method for detecting plant viruses in insect vectors is reverse-transcription quantitative polymerase chain reaction (RT-qPCR). In this study, the elongation factor-1 alpha (EF-1α) gene and acidic p0 ribosomal protein (RPAP0) gene were confirmed to be suitable reference genes for RT-qPCR normalization in viruliferous T. citricida aphids using the geNorm, NormFinder, and BestKeeper tools. Then the relative CTV titer in aphids (T. citricida) at different post-acquisition feeding times on healthy plants was quantified by RT-qPCR using EF-1α and RPAP0 as reference genes. The relative CTV titer retained in the aphids gradually decreased with increasing feeding time. During the first 0.5 h of feeding time on healthy plants, the remaining CTV titer in aphids showed about 80% rapid loss for the highly transmissible isolate CT11A and 40% loss for the poorly transmissible isolate CTLJ. The relative CTV titer in aphids during more than 12 h post-acquisition times for CT11A was significantly lower than at the other feeding times, which is similar to the trend found for CTLJ. To our knowledge, this is the first report about the relative titer variation of CTV remaining in T. citricida at different post-acquisition feeding times on healthy plants.

• The Plant Pathology Journal
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• 제35권1호
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.