• Journal of the Korean Society for information Management
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• v.37 no.4
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• pp.207-223
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• 2020

This study aims to review the possibility of OA to international journal articles cited by researchers of the library and information science field in Korea. For this, the international journal articless cited to the articles (1,543) of the Korean Society for Information Management were collected, and the investigation was carried out regarding the OA policy of the international journals and the status of journals that can be open to the public through the OA according to the OA policy. In addition, this study analyzed the actual accessibility by utilizing the OA finders (Google Scholar, Unpaywall, OA Button). The analysis result indicated that the majority of the international journals were using the green OA policy. Also, 1,476 journal articles which is 95.4% of the total international journal articles were allowed to be accessed officially with the OA. The results of reviewing the actual accessibility rate of the journal articles open to the public through the use of the OA finders indicated that accessibility was up to 68% when using Google Scholar, and the maximum accessibility rate was 72% when mixing the OA finders. Among the OA finders, Google Scholar had the greatest OA accessibility rate, but it was desirable to mix the OA finders in order to expand the OA accessibility rate to the maximum level.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.287-295
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• 2022

Citrus tristeza virus (CTV) is efficiently transmitted in a semi-persistent manner by the brown citrus aphid (Toxoptera citricida (Kirkaldy)). Currently, the most sensitive method for detecting plant viruses in insect vectors is reverse-transcription quantitative polymerase chain reaction (RT-qPCR). In this study, the elongation factor-1 alpha (EF-1α) gene and acidic p0 ribosomal protein (RPAP0) gene were confirmed to be suitable reference genes for RT-qPCR normalization in viruliferous T. citricida aphids using the geNorm, NormFinder, and BestKeeper tools. Then the relative CTV titer in aphids (T. citricida) at different post-acquisition feeding times on healthy plants was quantified by RT-qPCR using EF-1α and RPAP0 as reference genes. The relative CTV titer retained in the aphids gradually decreased with increasing feeding time. During the first 0.5 h of feeding time on healthy plants, the remaining CTV titer in aphids showed about 80% rapid loss for the highly transmissible isolate CT11A and 40% loss for the poorly transmissible isolate CTLJ. The relative CTV titer in aphids during more than 12 h post-acquisition times for CT11A was significantly lower than at the other feeding times, which is similar to the trend found for CTLJ. To our knowledge, this is the first report about the relative titer variation of CTV remaining in T. citricida at different post-acquisition feeding times on healthy plants.

• The Plant Pathology Journal
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• v.35 no.1
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.