• Korean Journal of Veterinary Research
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• v.48 no.4
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• pp.431-440
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• 2008

Brucellosis is one of the most important zoonosis in worldwide. As one of the control measures, attempts have been made to develop new diagnostic methods using filed isolates as a national policy in many countries. Currently, bovine brucellosis in Korea have been received attention in both public health and economical aspects due to sudden increase of outbreak. Based on the situation, we compared standard strain (B. abortus 1119-3) with field isolates to reveal the differences among them. Biological and biochemical charateristics, antibiotic resistance profiles, outer membrane proteins (OMPs) and lipopolysaccharide analysis of the strains were included in this study. For the diagnostic purpose, an attempt was made to find out a novel antigen from the Korean isolates by serological analysis. There were differences about 55 kDa, 36-38 kDa and 20 kDa in analysis of OMPs by SDS-PAGE and Western blot with positive sera ($\geq$ 1:400 in SAT titer). Also, a serological diagnostic method, ELISA was conducted using OMPs of the strains as novel antigen. Relationships between O.D. and SAT titer were analyzed using field sera showing different SAT titer. High correlation coefficient was observed between SAT titer and ELISA. Results from this study suggested that a new diagnsotic method should be developed using their own field isolates in each country.

• Journal of Cadastre & Land InformatiX
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• v.46 no.2
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• pp.27-42
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• 2016

Forest accounts for almost 64 percents of total land cover in South Korea. For inventorying, monitoring, and managing such large area of forest, application of remote sensing and geographic information system (RS/GIS) technology is essential. On the basis of spectral characteristics of satellite imagery, forest cover and tree species can be classified, and forest cover map can be prepared. Using three dimensional data of LiDAR(Light Detection and Ranging), tree location and tree height can be measured, and biomass and carbon stocks can be also estimated. In addition, many indices can be extracted using reflection characteristics of land cover. For example, the level of vegetation vitality and forest degradation can be analyzed with VI (vegetation Index) and TGSI (Top Grain Soil Index), respectively. Also, pine wilt disease and o ak w ilt d isease c an b e e arly detected and controled through understanding of change in vegetation indices. RS and GIS take an important role in assessing carbon storage in climate change related projects such as A/R CDM, REDD+ as well. In the field of climate change adaptation, impact and vulnerability can be spatio-temporally assessed for national and local level with the help of spatio-temporal data of GIS. Forest growth, tree mortality, land slide, forest fire can be spatio-temporally estimated using the models in which spatio-temporal data of GIS are added as influence variables.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.1
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• pp.28-35
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• 2010

In this paper, we designed and fabricated a ultra-wideband(UWB) bandpass filter with a good performance of a stopband, and a notched band in passband. The transformed equivalent circuit of the highpass filter was realized by distributed element. A wide-passband with 3-dB fractional bandwidth of more than 100 % was achieved by using optimum response of the HPF. For improving lower and upper stopband characteristic, a cross coupling between feed lines was employed, which was analyzed by desegmentation technique. In order to reject interference of Wireless LAN and Hyper LAN(5.15~5.825 GHz), the narrow notched(rejection) band was realized by a spurline. The fabricated BPF indicated the passband from 3.1 to 10.55 GHz and the flat group delay of less than 0.94 ns over the entire passband except the rejection band. The filter shown sharp attenuation both inside and outside the band and notched band from 5.2 to 6.12 GHz.

• Journal of Korean Society for Atmospheric Environment
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• v.21 no.2
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• pp.243-257
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• 2005

The effects of Traffic Control Program (TCP) on the ambient urban air quality of SO$_{2}$, NO$_{2}$, O$_{3}$, and PM$_{10}$ were evaluated in Seoul metropolitan area by using the lower atmospheric vertical stability index and daily mean wind speeds. The vertical stability index; temperature lapse rate between 1000 hPa and 850 hPa geopotential height fields, were used to identify daily vertical stability index during the 2002 World Cup period where traffic amount was reportedly reduced to half the number of vehicles. The indicated air quality levels of TCP days were then compared with those of the cases observed with analogous vertical stability during the recent 3 years from 2000 to 2002. The result indicates that the effect of TCP on the primary air pollutants are found to be approximately 39$\%$, 23$\%$ and 20$\%$ lower for SO$_{2}$, NO$_{2}$ and PM$_{10}$, respectively. The secondary air pollutant; ozone, showed relatively smaller decreasing rate (13$\%$) of daily mean concentrations (even increased during the night time). The comparison of daily maximum or peak concentrations reveals that the pronounced decreasing effects of TCP on the ambient air quality for both primary and secondary air pollutants, suggesting that TCP is one of the effective strategies to control peak or higher concentrations for most urban scale air pollutants in and around the Seoul metropolitan area.

• Microbiology and Biotechnology Letters
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• v.41 no.4
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• pp.442-448
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• 2013

Lactic acid bacteria (LAB) are a representative group of probiotics and used in many fermented foods and beverages. Several recent studies have shown that LAB are present in makgeolli which is a traditional Korean alcoholic beverage. However, most LAB are intolerant of more than 6% (v/v) alcohol concentrations. For this reason, alcohol-tolerant LAB are isolated from kimchi, makgeolli and nuruk using alcohol containing selective media. After being cultured in MRS broth containing 13% (v/v) alcohol, the two strains which showed the highest increasing O.D values, were finally selected. As results of 16S rRNA gene sequencing and biochemical characterization using an API kit, the two species were identified as Pediococcus acidilactici K3 and S1. In addition, the identified two strains produced bacteriocins against Staphylococcus aureus. When compared with the P. acidilactici type strain, the two selected strains possessed two to three time higher growth on 12-13% (v/v) alcohol containing MRS broth. The viability of P. acidilactici K3 and S1 when inoculated in makgeolli and stored at $10^{\circ}C$ did not decrease through a period of one month indicating that the selected strains can be used for LAB containing makgeolli.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.10
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• pp.1825-1832
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• 2000

Column experiments were conducted by using soil columns, to investigate feasibility and efficiency of in-situ ozone enhanced remediation for diesel-contaminated soil. The injection of gaseous ozone into soil column revealed the enhanced decomposition of ozone due to the catalytic reaction between ozone and metal (e.g., Fe, Mn etc.) oxides as evidenced by as much as 25 times shorter half-life of ozone in a sand packed column than in a glass beads packed column. Substantial retardation in the transport of and the consumption of ozone were observed in the diesel contaminated field soil and sand packed columns. After 16 hrs ozonation, 80% of the initial mass of diesel (as diesel range organic) concentration of $800{\pm}50mg/kg$, was removed under the conditions of the flow rate of 50mL/min and $6mg-O_3/min$. Whereas, less than 30% of diesel was removed in the case of air injection. Analysis of the residual TPH(total petroleum hydrocarbon) and selected 8 aliphatics of diesel compounds in the inlet and the outlet of the column confirmed that diesel nonselectively reacted with ozone and then shifted to lower carbon numbered molecules. Water content also was found to be an important parameter in employing ozone to the hydrocarbon-contaminated soil.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.499-506
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• 2011

The purpose of this study was to search available resources for new natural colorants. The extraction efficiency of colorants from black cowpea seed coats and their storage stability were examined according to the various extraction and storage conditions in this study. The results obtained were as follows: the optical density (O.D.) values of the extracted colorants increased with increasing extract time and temperature. Extraction at pH 4 was seen to be the most efficient among the various pH conditions. The color of the extract solutions were seen to change with variation in pH, for example, anthocyanins display color changes from orange-red, to orange, to blue, to greenish-blue at pH 3.0, 4.0-6.0, 7.0 and 9.0-11.0, respectively. The color changes of the extract solutions over various storage periods were determined using UV/Vis spectra these color changes indicate characteristic absorption patterns and a discoloration index which indicates the rate of absorbance (532 nm/454 nm). Methionine addition influenced the storage stability of the colorant solutions and this addition led to better storage stability than non-addition. In paper chromatography of juice extracted colorant, a long stripe was seen on development. Among three colorants obtained via paper chromatography according to development rate, at least two different colorants were mixed indicated by the appearance, or not, of a shoulder at 552 nm depending on the extent of development.

• Journal of Life Science
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• v.19 no.3
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• pp.378-383
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• 2009

Agrobacterium tumefaciens-mediated transformation procedure for the phalaenopsis orchid, established by using Protocorm-like bodies (PLBs), was aimed at the introduction of target genes into individuals with divergent genetic backgrounds. PLBs obtained from the axillary bud of a peduncle were maintained on a hyponex medium supplemented with 1 g/l of activated charcoal, 30 g/l of sucrose and 0.1 mg/l thiamine. The multiplication rate of PLBs was about 90% in case of subculture PLBs to be cut transversely into 1/3 part from top position. The PLBs were inoculated with Agrobacterium strain EHA105 harboring both $\beta$-glucuronidase (GUS) and hygromycin-resistant genes for 20 minutes after dipping treatment. Transformation efficiency was the highest with a Agrobacterium culture medium and dipping treatment of O.D. 0.8. Newly induced PLBs were put on selection medium containing 1 mg/l hygromycin for 2 months. Hygromycin-resistant phalaenopsis plants that regenerated after the selection culture of PLBs showed histochemical blue staining due to GUS. Transgene integration of the hygromycin-resistant plants was confirmed by PCR and Southern blot using GUS specific primers and probe.

• Journal of Korea Port Economic Association
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• v.23 no.4
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• pp.71-90
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• 2007

To induce container cargos to Gunsan port, we should struggle on securing the quantity of goods transported from the competitive Jeonbuk and Chungcheong areas by reducing transportation expenses by land, and make an activating plan for the international exchange between Gunsan port and nearby ports in China. As a result, if the plan to induce container cargos to Gunsan port is activated, the regular liners will stop at Gusan port. So the new sea route will be opened so that consignors can ship cargos in the proper time. The consignors will use Gunsan port frequently because of the reduction of distribution expenses. To do so, for the first, many incentives should be implemented to attract container cargos. In addition, various facilities should be fully equipped and improved. This paper is to improve the problems for which consignors, the shipping lines and forwarders may feel uncomfortable when they use Gunsan port. According to the research on the actual condition, the biggest problem in Gunsan port is the depth of water. The management, relation system, infrastructure and information system of Gunsan port are also insufficient. Therefore, this paper will make an alternative plan to improve these problems and help the container cargos induce to Gunsan port.

• Korean Journal of Materials Research
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• v.8 no.11
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• pp.1055-1060
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• 1998

Thermoelectric properties of ($Pb_{1-x}Sn_x$)Te ($0\leq{x}\leq{0.4}$) alloys, fabricated by mechanical alloying and hot pressing, were investigated with variation of the SnTe content. For the hot-pressed PbTe and ($Pb_{0.9}Sn_{0.1}$)Te. transition from p-type to n-type occurred at $200^{\circ}C$ and $300^{\circ}C$, respectively. However, the specimens containing SnTe more than 0.2mole exhibited p-type conduction up to 450'C. In extrinsic conduction region, the Seebeck coefficient and electrical resistivity of the hot-pressed ($Pb_{1-x}Sn_x$)Te decreased with increasing the SnTe content. The temperature at which the hot-pressed (Pbl-,Sn,)Te exhibited a maximum figure-of-merit was shifted to higher temperature with increasing the SnTe content The hot-pressed (Pbo ,Sno dTe exhibited a maximum figure-of-merit of $0.68\times10_{-3}/K$ at $200^{\circ}C$.