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Flying Aphid Population at the Horticultural Experiment Station, Suweon (원예시험장 주변의 진딧물)

  • Paik Woon Hah;Song Ki Won;Choi Seong Sik
    • Korean journal of applied entomology
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    • v.13 no.1 s.18
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    • pp.25-31
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    • 1974
  • This survey was aimed to accumulate basic data of aphid population at the Horticultural Experiment Station at Suweon. The yellow pan traps were setted at five locations (Fig.1.), and ran from May 1 to October 31. 1970. About one hundred and twenty species of aphids were trapped, including 24 species of plant vims vectors. Of these, dominant species were as follows: (Asterisk shows virus vector) Aphid species No. of catches * Aphis spiraecola PATCH 2,635, * Aphis craccivora KOCH 2,377, * Myzus persicae SULXER 2,111, Capitophorus hippophaes javanicus H.R. LAMBERS 2,051, Anoecia fulviabdominalis SASAKI 1,480, * Aphis gossypii GLOVER 867, * Macrosiphum avenae FABRICIUS 859, Cervaphis quercus TAKAHASHI 692, * Lipaphis erysimi KALTENBACH 645, Pleotrichophorus chrysanthemi THEOBALD 489, The above 10 species consisted $76.5\%$ of total catches and the 24 vector species consisted $55.5\%$. The curve of the seasonal occurrence of flying aphids at Horticultural Experiment Station shows bimodal, typical for the temperate region. The total number of trapped aphids at the Station from May to September, 1970, were less than that of average yearly catches at the College of Agriculture from 1967 to 1970. Thi, low numbers at Horticultural Experiment Station may attribute to the frequent spraying of insecticides from Spring to Summer on growing crops there. But the aphids population increase suddenly in the middle of October. This might be resulted from cease of insecticide applications and migration of aphids from summer host to winter host plants.

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An Analysis of Preservice Earth Science Teachers' Mental Models about Coriolis Force Concept (예비 지구과학 교사의 전향력 개념에 대한 정신모형 변화 분석)

  • Kim, Eunju;Lee, Hyundong;Lee, Hyonyong
    • Journal of The Korean Association For Science Education
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    • v.36 no.3
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    • pp.423-434
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    • 2016
  • The purpose of this study is to investigate preservice earth science teachers' mental models through applications of Coriolis force experiment apparatus. After the root of preconception was examined by face to face interviews based on the questionnaire, five preservice earth science teachers were finally selected for this study. The mental models about concept of Coriolis force was classified into naive mental model, static unstable mental model, dynamic unstable mental model, and scientific mental model through the result of individual interviews and their drawings. According to the mental model analysis about Coriolis' force conception, students C and M showed naive mental model about concept of Coriolis force before experiment. After the experiment, student M's model changed to static unstable mental model. Student C's model improved to dynamic unstable mental model. In adiition, students D and O's model improved from static unstable mental model to dynamic unstable mental model. In the case of student B, the dynamic unstable mental model was maintained after the experiment, however, student B's preconception changed to scientific concept. It turned out that a change occurred from low mental model level to integrated mental model after the application of the developed Coriolis' force experiment apparatus. According to the results, national curriculum is similar to static unstable mental model and the result of developed Coriolis' force experiment apparatus is similar to dynamic unstable mental model. It is suggested that it become the theoretical foundation to develop more comfortable and advanced Coriolis force experiment apparatus by improving the experiment apparatus.

Purification and Some Characteristics of the Proteolytic Enzyme in Fruitbody of Neungee [Sarcodon aspratus (Berk.) S. Ito] (능이 [Sarcodon aspratus (Berk.) S. Ito]중 단백질(蛋白質) 가수분해(加水分解) 효소(酵素)의 정제(精製) 및 성질(性質)에 관하여)

  • Lee, Tae-Kyoo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.3
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    • pp.276-285
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    • 1986
  • This study was undertaken to investigate the characteristics of the proteolytic enzyme extracted from Neungee mushroom [Sarcodon aspratus (Berk.) S. Ito]. The enzyme was purified by using Tris-acryl CM-cellulose ion exchange, gel filtration on Ultrogel AcA 54, Hydroxy apatite column chromatography and preparative isoelectic focusing. The specific activity of the purified enzyme increased 8 times as compared with that of the crude enzyme. The enzyme was homogeneous on polyacrylamide gel electrophoresis (PAGE). The optimum pH was 10.1, indicating the enzyme to be alkaline protease and the optimum temperature was $57^{\circ}C$. The enzyme was stable at temperatures lower than $50^{\circ}C$and at pH values ranging from 4.0 to 10.8. However, the enzyme activity decreased by 26 and 65% at 60 and $65^{\circ}C$, respectively, when incubated for 30 minutes. The enzyme activity was activated by $Mn^{++}$ and inhibited by $Cu^{++}$ and $Hg^{++}$. The enzyme was consisted of monomer and its molecular weight estimated to be about 30,100 when determined by sodium dodecyl sulfate PAGE. Isoelectric point of the enzyme was determined to be 9.80.

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Ecological Studios on the Bark Beetles on Plum and Apricot (자두와 살구나무에 기생하는 나무좀의 생태에 관한 연구)

  • Yoon J. K.;Kim K. C.;Cheon S. J.;Kim Y. S.
    • Korean journal of applied entomology
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    • v.21 no.2 s.51
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    • pp.78-86
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    • 1982
  • The ecological studies were conducted to identify some bark-beetles on apricot and plum trees in Jeonnam province. Harmful bark-beetles caught from apricot and plum trees were identified as Scolytus seulensis. Xyleborus atratus., X. germanus, X. rubricollis and X. saxeseni. The seoul barkbeetle emerged during the period from early May to late October $1980\~1981$, it happens twice in a year, with the peak July 10 and August 25 in 1950, July 15 and August 20 in 1981. A daily peak emerged for adults was at 11 AM to 1 PM. Distribution of holes on the apricot stems varied; more than 100 holes per meter in Henderson (Apricot) and Y-49057 (Apricot), 12 holes per meter in Derbyroyal (Apricot). Comparatively more holes were found in the European variety, and the cardinal distribution was in the order of east, south, west and north. The longer the longth of the mother-gallery, the greater number of egg-gallery found. Optimum temperature for mating was $27^{\circ}C$. The time for mating was longer during May-June than July-August. The fruit weight of damaged plum tree reached 12.25g around four weeks after flowering; where as that of normal tree increased up to 63.5g at harvest time. The natural enemy of the seoul bark beetle was Eurytoma sp. They were found as mature larva or pupa and they emerged from early May to the middle of June. Adult longevity was about a week.

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Production of Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid by Pseudomonas sp. HJ (Pseudomonas sp. HJ에 의한 Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid의 생산)

  • 손홍주;민관필이상준
    • KSBB Journal
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    • v.10 no.4
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    • pp.349-356
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    • 1995
  • To produce PHA(polyhydroxyalkanoic acid) from microbr, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source (glucose) that was not generally considered as precursor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were $37^{\circ}C$ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH4) 2SO4 0.2% as a nitrogen source, K2HPO4 0.3%, and KH2PO4 0.45%. TO investigate she optimal condition for PHA production two-step cultivation method was employed. PHA production was inducted by deficiency of NH4+, SO4-2, Mg+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH4+ stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to pyoduc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. The contents of PHA and she proportions of hydroxyvalerate monomer units varied depending on the carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by HNMR analysis.

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Effects of Endoglucanase and Exoglucanase from Trichoderma viride on Brightness and Physical Properties of Deinked Old Newsprint (Trichoderma viride로부터 분리한 Endoglucanase 및 Exoglucanase가 탈묵 펄프의 백색도 및 물리적 강도에 미치는 영향)

  • 김동원;정영규장영훈손기향
    • KSBB Journal
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    • v.11 no.6
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    • pp.718-725
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    • 1996
  • Old newsprint was deinked with endoglucanase, exoglucanase and their various compositions from Trichoderma viride. The yield decreased with an increase in enzyme concentration. Especially, it was the lowest in the treatment of endo-exo mixture(1:1). It may be regarded as a synergistic actions of the cellulase components. The brightness was the highest in pulp deinked with endo-exo mixture(1:1). Maximum brightness was observed when 0.5mg/mL of the endo-exo mixture was used. The physical strength increased with increasing concentration in exoglucanase. But, it decrease with increasing concentration in endoglucanse and endo-exo mixture(1:1). Also, we investigated the yield, brightness and physical strength of endoglucanase in combination with exoclucanase(12:1, 8:1, 4:l, 1:1, 1:4, 1:8, 1:12). Maximal deinking conditions, obtained at a specific optimal ratio of endoglucanase to exoglucanse are as follow ; 12:1 for yield, 12:1 for brightness, 4:1 for tensile strength, 12:1 for bursting strength, and 8:1 for tearing strength. These results indicated that the deinking depended largely upon the action of endoglucanase. Exoglucanase was occupying more than 60% of the total crude cellulase contents. Therefore, the most effective deinking must repress the action of exoglucanase.

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Quantitative Structure-Toxicity Relationships (QSTRs) of Fungicidal Phenylthionocarbamate Derivatives (살균성, Phenylthionocarbamate 유도체들의 정량적인 구조와 독성과의 관계)

  • Sung, Nack-Do;Yang, Sook-Young;Park, Kwaun-Yong
    • Korean Journal of Agricultural Science
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    • v.28 no.1
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    • pp.33-40
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    • 2001
  • The authors attempted to derive a comprehensive quantitative structure-toxicity relationships (QSTRs) between various physicochemical parameters of phenyl substituents in fungicidal phenylthionocarbamate derivatives and toxicity evaluated using TOPKAT calculation. On the basis of this approach we made preditions for toxicity values for not yet tested substances with respect to these systems. The results suggested that the optimal values, $(B_2)_{opt.}=1.54_{\AA}$(Ames mutagenicity), $(R)_{opt.}=0.16$ (car-cinogenicity of male rat), $(\pi)_{opt.)=0.16$ (carcinogenicity of male mouse), $({\varepsilon}LOMO)_{opt}=-0.52e.v.$ ($LD_{50}$ of rat oral), $(B_3){opt.}=1.54_{\AA}$(chronic LOAEU), $(logP)_{opt.}=4.25$ ($LC_{50}$ of Fathead minnow) and $({\sigma})_{opt}=-0.68$ ($EC_{50}$ of Daphnia magna) of phenyl substituents were strongly correlated with the acute and chronic toxicities.

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Study on maintaining healthy body and changes of human body by circadian rhythm (인체(人體)의 일주리듬에 따른 변화(變化)와 건강법(健康法)에 관한 연구(硏究))

  • Jeong, Sang Jee;Kang, Jung Soo
    • Journal of Haehwa Medicine
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    • v.12 no.1
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    • pp.103-121
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    • 2003
  • Human being can't live without nature, then the changes of nature affect human body. It means that human body has corresponding changes to the KI(vital energy) of nature. There is a stream of changes in human body which circulate mysteriously and punctually by the laws of nature. If this stream of changes fits into human's life style, it would be most effective. It has a certain mode continuously. So if a person has a habit fitting into it, he will get the healthiest body. Then the researcher tries to explain the changes in human body by the time, mainly focused on within 24 hours. it is showing not only the oriental view, but also the western's. The researcher can find the coincidence as followings. At In-Si(3-5 am), the body function and the body temperature get to the bottom, therefore it's good for him to wake up and to run the vital energy. At Sa-Si(9-11 am), the patience on pain anxiety and the psychic concentration get to the top, he'd better start the work. At O-Si(11am-1pm), the heart energy has a vital move, then the blood concentration of Hb(hemoglobin) gets to the top. At Mi-Si(1-3 pm), the muscle strength, the squeeze, and the breathing rate increase. The reflex nerve sensitivity gets to the top. Creativity, observation, and working efficiency go high, so it's time to work hard. At Hae-Si(9pm-1am), the body function falls, sleeping is needed. At Chuck-Si(1-3 am), the cell spontaneity gets to the top, immune lymphocyte moves actively, and the blood concentration of growth hormone gets to the top. These are liver's work. In west, there has been active studies on how to reduce the side effect by using a person's bio-rhythm based on the 'time treatment', and how to reorganize the bio-rhythm by using the machine and the age resistance based on the 'bio-watch'. Though the 'time treatment' means something, the artificial resistance on bio-rhythm seems to give bad effects to human body. If a person lives by regimen of oriental medicine, he will maintain the healthiest body. Regimen is that human body follows the laws of nature, and moves its mysterious, Punctual and periodical changes.

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Survey on Soybean Parasitic Nematodes (대두증산을 위한 기생선충 조사)

  • Choi Young-Eoun;Choi Dong-Ro
    • Korean journal of applied entomology
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    • v.22 no.4 s.57
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    • pp.251-261
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    • 1983
  • Survey on soybean parasitic nematodes has been undertaken in order to get an ideas about the distribution of nemic fauna. Total 71 soil samples were collected from soybean fields in Gyeongbug and Chungbug provinces. Eighteen species belonging to fourteen different genera were found associated with soybean, Glycine max. The results were as follows: Aphelenchus avenae, Circonemoides informis, Ditylenchus dipsaci, Helicotylenchus pseudorobustus, Heterodera glycines, Hirschmanniella imamuri, Meloidogyne hapla, Meloidogyne incognita, Paratylenchus pandus, Pratylenchus thornei, Pratylenchus vulnus, Psilenchus hilarulus, Rotylenchus sp. Tylenchorhynchus clay toni, Tylenchorhynchus dubius, Tylenchorhynchus nudus, Tylenchus sp. Xiphinema americanum were found in soybean fields. In frequency of occurring nematodes by localities, soybean cyst nematode was higher in Chungbug by $70\%$, while $50\%$ in Gyeongbug. But rook-knot nematode was higher in Gyeongbug by $54.1\%$, while $19.1\%$ in Chungbug. In density of nematode, Heterodera was the most dominant as 200.4 populations per 100ml soil, Meloidogyne as 107.1 populations, Rotylenchus, Xiphinema, Aphelenchus in decreasing order. In survey of loss, density of soybean cyst nematode in heavily infested fields was 155 cysts per 100ml soil while 35 cysts in slightly infested fields. Seventy percent of soybean yields was decreased when comparing with average 64.2kg/10a in heavily infested fields and average 209.5kg/10a in slightly infested. Correlation coefficients among number of soybean cyst and yields, pod weight, number of pod was negative as r- 0.57, 0.47, 0.38 over all localities.

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Establishment and Validation of Gold Amalgamation Method for the Quantitation of Thimerosal in Biological Products (생물학적제제의 치메로살 함량 정량을 위한 가열기화 아말감 흡광도법의 확립 및 검증)

  • Kim, Byung-Chul;Kim, Do-Keun;Hong, Sung-Hwa;Kim, Yeon-Hee;Lim, Jong-Mi;Won, Yun-Jung;Kim, Seok-Hwan;Hong, Ji-Young;Yun, Young-Min;Kim, Jae-Ok
    • YAKHAK HOEJI
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    • v.55 no.4
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    • pp.284-288
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    • 2011
  • The test method for biologics of lot release system is based on 'Test procedure and specification for biological products,' generally, thimerosal content is measured by chemical analysis using O.D. In this study, the comparative analysis was carried out using the gold amalgamation method for thimerosal content was compared to the existing methods, which are described above. The gold amalgamation method, which uses atomic absorption spectrophotometry, was meets all the method validation acceptance criteria. It is considered to be proper as the assay and identification test for thimerosal. In this study, the comparative analysis was performed three times. As a result, gold amalgamation method is more convenient and easy to perform as this assay doesn't have pre-treatment procedure. Also this assay showed good precision and reproducibility compared to the conventional method. Therefore, it is appropriate to alternate the assay method of thimerosal from the conventional chemical analysis to gold amalgamation method to improve the credibility of lot release system and the quality control of biologics, by standardizing test method.