• 대한수의학회지
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• 제50권3호
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• pp.239-246
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• 2010

Among 203 avian pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis in korea, 14 isolates were selected from total 68 isolates transferred R plasmid and classified into 5 groups on the basis of antimicrobial minimal inhibitory concentration (MIC) pattern, farm source and O serotype. An association between clonal origin and R plasmid of them was investigated by R plasmid profile, restriction endonuclease analysis and pulsed-field gel electrophoresis (PFGE). The strains that showed the same or very similar antimicrobial MIC pattern, but different farm source and O serotype, revealed different PFGE pattern, which seemed to be different clonal origin. And the strains that showed the same MIC pattern and O serotype, revealed different PFGE pattern, seemed to be originated from different clone. Also the strains showing the same MIC pattern and farm source, but different O serotype, revealed to be different clonal origin. The strains that showed the same or similar MIC pattern, farm source, and O serotype, revealed identical or similar PFGE pattern, which seemed to belong to be one clone. Meanwhile, horizontal transfer of R plasmid seems to be common in APEC with regardless of O serotype and clone of the strains. These results indicate that rapid and accurate epidemiological survey of APEC can be possible by the combination of O serotyping, plasmid profiling and PFGE analysis following the classification of them into groups of antimicrobial drug resistance pattern.

• Restorative Dentistry and Endodontics
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• 제21권1호
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• pp.121-135
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• 1996

Actinomyces are Gram-positive, non-acid-fast, anaerobic or microaerophilic filamentous bacteria. These organisms are frequently detected from infected root canals and periapical lesion. The purpose of this study was to use indirect immunofluorescence to determine the prescence of select Actinomyces species in a survey of teeth associated with periapical lesion, to clarify the relationship between clinical symptoms of periapical lesions and the Actinomyces species and to study on the cross reaction among Actinomyces. Actinomyces israelii serotype I (ATCC 12102), Actinomyces israelii serotype II (ATCC 29322), Actinomyces viscosus serotype II (ATCC 19246), Actinomyces naslundii serotype I (ATCC 12104) were cultured in anaerobic condition. Rabbit antisera were prepared by intravenous injection of formalized whole cells. Indirect immunofluorescence method was used to achieve the purpose. The following results were obtained. 1. There was a relationship between Actinomyces and periapical disease. 2. A. israelii serotype I, II were frequently identified with Indirect Immunofluorescence and most often assosiated with periapical disease. In culture finding, there was no significant difference between each group. 3. Indirect Immunofluoresence is both more sensitive and more rapid than culture for identification of Actinomyces species in patients with periapical lesion. 4. A. israelii serotype I, II was highly isolated in infected root canals with local swelling, A. naslundii serotype I was highly isolated in those with foul odor, and A. israelii serotype I was found in higher frequncy in those with exudate than other bacteria. 5. In the Indirect Immunofluorescence (1 : 320), A positive cross reaction was obtained between A. israelii serotype I and A. israelii serotype II, also, A. viscosus serotype II and A. naslundii serotype I. There was no cross reaction between A. israelii serotype I, II and A. viscosus serotype II, A. naslundii serotype I.

• 한국수산과학회지
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• 제51권3호
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• pp.254-261
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• 2018

From 2014 to 2017, mortalities of seawater-cultured rainbow trout Oncorhynchus mykiss, were observed in the Goheung and Jeju areas of Korea, with Vibrio anguillarum (seven strains: RT1, 2, 3, 4, 5, 6, and 7) identified as the etiological agent. The phenotypic (based on API 20NE, API ZYM, and E-test kits), serotypic (slide agglutination tests with O1, O2, O3, O4, and O7 antisera), and genotypic (16S rRNA and ompU sequencing) characteristics of the seven RT strains were analyzed and compared to those of seven additional V. anguillarum stains (SF, isolated from sweet fish; FM, isolated from flathead mullet; ATCC43305; ATCC43311; ATCC43307; ATCC43308; and KCTC2711). The phenotypes of the RT strains showed variance, while the slide agglutination tests of the RT1-7, SF, and FM strains all showed positive reactions with serotype O1 antiserum. The 16S rRNA and ompU sequences of the RT1-7, SF, and FM strains were affiliated with V. anguillarum ATCC43305 (Serotype O1), but the ompU sequence of the SF strain differed from those of the RT1-7, FM, and ATCC43311 strains, including one amino acid substitution. We thus confirmed that serotype O1 V. anguillarum, with multiple phenotypes, continues to infect seawater-cultured rainbow trout in Korea.

• 미생물학회지
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• 제29권3호
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• pp.199-202
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• 1991

The O serotypes of uropathogenic Escherichia coli isolated in Korea were studied using a complete set of rabbit O antisera raised with reference O antigen type strains of E. coli. The distribution of "O" serotypes found in this survey was grossly similar with the prevalence of "O" types observed in other parts of the world, and some differences were also noted. A total of 31 "O" serotypes were identified and the most frequent serotype associated with urinary tract infections was O75(11.5%), which was followed by O6(7.4%), O10 and O40(5.7%, respectively).0 and O40(5.7%, respectively).

• Restorative Dentistry and Endodontics
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• 제20권2호
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• pp.645-654
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• 1995

Porphyromonas endodontalis is a black-pigmented anaerobic Gram negative rod which is associated with endodontal infections. It has been isolated from infected dental root canals and submucous abscesses of endodontal origin. DNA probe is an available alternative, offering the direct detection of a specific microorganism. Nucleic-acid probes can be off different types: whole different: whole-genomic, cloned or oligonucleotide probes. Wholegenomic probes are the most sensitive because the entire genome is used for possible hybridization sites. However, as genetically similar species of bacteria are likely to be present in specimences, cross-reactions need to be considered. Cloned probes are isolated sequences of DNA that do not show cross-reactivity and are produced in quantity by cloning in a plasmid vector. Cloned probes can approach the sensitivity found with whole-genomic probes while avoiding known cross-reacting species. Porphyromonas endodontalis ATCC 35406 (serotype $O_1K_1$) was selected in this experiment to develop specific cloned DNA probes. EcoR I-digested genomic DNA fragments of P. endodontalis ATCC 35406 were cloned into pUC18 plasmid vector. From the E. coli transformed with the recombinant plasmid 4 clones were selected to be tested as specific DNA probes. Restriction-digested whole-genomic DNAs prepared from P. gingivalis 38(serotype a), W50(serotype b), A7A1-28(serotype C), P. intermedia 9336(serotype b), G8-9K-3(serotype C), P. endodontalis ATCC 35406(serotype $O_1K_1$), A. a Y4(serotype b), 75(serotype a), 67(serotype c), were each seperated on agarose gel electrophoresis, blotted on nylon membranes, and were hybridized with digoxigenin-dUTP labeled probe. The results were as follows: 1. Three clones of 1.6kb(probe e), 1.6kb(probe f), and 0.9kb(probe h) in size, were obtained. These clones were identified to be a part of the genomic DNA of P. endodontalis ATCC 35406 judging from their specific hybridization to the genomic DNA fragments of their own size on Southern blot. 2. The clones of 4.9kb(probe i) was identified to be a part of the genomic DNA of P. endodontalis ATCC 35406. but not to specific for itself. It was hybridized to P. gingivalis A7A1-28, P. intermedia G89K-3.

• 한국동물위생학회지
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• 제32권4호
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• pp.315-323
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• 2009

Foot-and-mouth disease (FMD) virus exists in seven serotypes and is known to be a highly contagious disease that is hard to eradicate from the world. The O, A, Asia1 and SAT2 serotypes commonly infected cattle, sheep and goats during 2007~2009 throughout the world. In particular, the outbreak of the Asia1 serotype in China appeared in all areas from 2005 and is still present. Surprisingly, in 2009, Taiwan reported the first outbreak of the type O serotype since 2001. Then type A appeared in China for the first time since the early 1960s. The virus shows a close relationship to the viruses from Southeast Asia suggesting one or more recent introductions into China in the OIE reports. Recently the subtype of A/Iran05 spread to nearby countries exhibiting genomic evolution. The use of molecular epidemiology is an important tool in understanding and consequently controlling the FMD virus. The phylogenetic analysis with VP1 gene was especially useful for molecular epidemiological studies and showed the same pattern which matches with serotype classification. This paper describes basic information about the disease, and the serotype-specific characteristics and evolution to perform molecular epidemiological analysis. Furthermore, we show the importance of the genetic evolution on the FMD serotypes in global surveillance and molecular epidemiology of FMD for outbreak investigation.

• 한국식품과학회지
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• 제32권6호
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• pp.1336-1340
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• 2000

서울등 5개지역에서 수거한 냉동식품 624건을 대상으로 Y. enterocolitica를 분리하였다. 분리대상 식품군 중 해산물가공품중에서 Y. enterocolitica의 분리율(9.1%)이 가장 높았고, 다음으로 식육가공품(7.7%), 만두류(3.5%), 기타 냉동식품(3.4%)의 순이었으며 냉동피자류(1.7%)는 분리율이 가장 낮았다. 그리고 상반기와 하반기의 분리율이 차이를 보였는데 각각 1.6%와 9.6%로 하반기가 상반기에 비하여 분리율이 6배가 높았다. 냉동식품에서 분리된 Y. enterocolitica 균주는 모두 35개(5.6%)였고, 분리균주의 serotype은 O:5(9균주)와 O:1,2(1균주)이었다. 이외의 나머지 25개 균주는 본 연구에서 사용한 항혈청에 응집반응을 보이지 않아 혈청형을 typing할 수 없었다. 그리고 이들 균주를 biotype한 결과 모두 비병원성인 biotype 1A이었으며 중합효소연쇄반응(PCR)으로 확인 결과 또한 모두 비병원성으로 판명되었다.

• 한국어병학회지
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• 제1권1호
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• pp.45-50
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• 1988

1985년(年) 7월(月)부터 1986년(年) 12월사이에 양식(養殖)방어, 참돔 및 돌돔으로 부터 분리(分離)한 26균주(菌株)의 Vibrio anguillarum에 대한 혈청학적(血淸學的) 동정(同定)의 실시(實施)하였다. 이 분리균(分離菌)들을 Kitao et al. (1983)이 보고(報告)한 6가지 혈청형(血淸型)의 V. anguillarum에 대하여 내열성(耐熱性) O항원(抗原)을 이용(利用)하여 상호응집반응(相互凝集反應)시켜본 결과(結果), 1. 본(本) 분리균(分離菌)은 모두 하나의 동일(同一)한 혈청형(血淸型)을 가지고 있었으며 Kitao et al. (1983)이 보고(報告)한 혈청형(血淸型)C(대표균주(代表菌株) PT-213)와 만이 응집반응(凝集反應)하였다. 2. 본(本) 분리균(分離菌)은 모두 PT-213혈청(血淸)에 대하여 동일(同一)한 2중(重)의 침강선(沈降線)을 형성(形成)하였다. 3. 본(本) 분리균(分離菌)은 모두 PT-213의 인자항혈청(因子抗血淸)에 대하여만 특이반응(特異反應)을 하였다. 따라서 본(本) 분리균(分離菌)들의 혈청형(血淸型)은 모두 Kitao et al.(1983) 이 보고(報告)한 혈청형(血淸型)C와 동일한 것으로 판단되었다.

• Journal of Veterinary Science
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• 제24권3호
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• pp.40.1-40.6
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• 2023

Analysis of the VP1 gene sequence of the foot and mouth disease virus (FMDV) is critical to understanding viral evolution and disease epidemiology. A standard set of primers have been used for the detection and sequence analysis of the VP1 gene of FMDV directly from suspected clinical samples with limited success. The study validated VP1-specific degenerate primer-based reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and sequencing of serotype O FMDV lineages circulating in India. The novel degenerate primer-based RT-PCR amplifying the VP1 gene can circumvent the genetic heterogeneity observed in viruses after cell culture adaptation and facilitate precise viral gene sequence analysis from clinical samples.

• 한국식품영양과학회지
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• 제30권5호
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• pp.959-963
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• 2001

식품 가검물로부터 분리 동정된 L.monocytogenes에 대하여 황금추출물의 항균력을 검토하였다. 먼저 오렴실태를 조사하였는데, 검사대상 가검물 178검 중 30건이 검출되어 16.9%의 검출율을 보였다. 쇠고지에서 93건 중 13건(14.0%)이 검출되었고, 돼지고기에서는 29건중 9건(39.2%)이 검출되어 가장 높은 검출율을 나타내었다. 가공식품에 있어서 육제품인 소시지류 및 햄류와 유제품인 치즈, 시유등의 살균제품에서는 L.monocytogenes 균이 검출되지 않은 반면, 냉동식품인 분쇄가공품에서는 검사시료 12건중 2건(16.7%)이 검출되었다. 분리된 L.monocytogenes 30균주를 슬라이드응집법으로 혈청형으로 확인한 결과 30균주에서 serotype O-1은 23건(76.7%)이 확인되었으면 serotype O-4은 7건(23.3%)으로 나타났다. 식품에 있어서 생육관계를 조사하기 위하여 시판되는 무방부제햄을 시료로 분리균주들을 각각 접종한 다음 35$^{\circ}C$에서 0, 3, 6, 24, 48, 72, 96및 120시간 동안 배양하여 얻은 생육곡선에서 L.monocytogenes균주들 모두가 유도기, 대수기, 정지기, 사멸기를 거치는 전형적인 S자 모양의 생육곡선을 보였다. 식품에 대한 황금추출물의 항균작용을 알아보기 위하여 증기멸균된 무방부제햄과 동량긔 멸균생리식염수를 넣고 황금추출물의 농도가 0, 500, 1000 및 5,000 ppm(mg/kg)이 되도록 균질화시킨 시료에서 분리균주들을 각각 접종한 다음 35$^{\circ}C$에서 0, 3, 6, 24, 48시간 동안 배양하여 얻은 생육저해곡선에서 L.monocytogenes 균주들 모두가 유도기를 거친 다음 6시간이 경과된 후 황금추출물의 농도에 따라 시험균주의 균수 차이를 보였고 식품중의 L.monocytogenes에 있어서 생육이 억제되는 황금추출물 유효저해농도는 1000 ppm 이었다. 세포형태변화를 관찰하기위한 투과형전자현미경(TEM)상에서 황금추출물 처리로 인한 L.monocytogenes 균주들의 세포막기능의 파괴로 세포내용물들이 용출된 것을 볼수 있었다.