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Molecular epidemiologic analysis of pathogenic Escherichia coli isolated from poultry in Korea  

Sung, Myung-Suk (College of Veterinary Medicine, Kyungpook National University)
Kim, Jin-Hyun (College of Veterinary Medicine, Kyungpook National University)
Kim, Ki-Seuk (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Korean Journal of Veterinary Research / v.50, no.3, 2010 , pp. 239-246 More about this Journal
Abstract
Among 203 avian pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis in korea, 14 isolates were selected from total 68 isolates transferred R plasmid and classified into 5 groups on the basis of antimicrobial minimal inhibitory concentration (MIC) pattern, farm source and O serotype. An association between clonal origin and R plasmid of them was investigated by R plasmid profile, restriction endonuclease analysis and pulsed-field gel electrophoresis (PFGE). The strains that showed the same or very similar antimicrobial MIC pattern, but different farm source and O serotype, revealed different PFGE pattern, which seemed to be different clonal origin. And the strains that showed the same MIC pattern and O serotype, revealed different PFGE pattern, seemed to be originated from different clone. Also the strains showing the same MIC pattern and farm source, but different O serotype, revealed to be different clonal origin. The strains that showed the same or similar MIC pattern, farm source, and O serotype, revealed identical or similar PFGE pattern, which seemed to belong to be one clone. Meanwhile, horizontal transfer of R plasmid seems to be common in APEC with regardless of O serotype and clone of the strains. These results indicate that rapid and accurate epidemiological survey of APEC can be possible by the combination of O serotyping, plasmid profiling and PFGE analysis following the classification of them into groups of antimicrobial drug resistance pattern.
Keywords
avian pathogenic Escherichia coli (APEC); molecular epidemiological analysis; O serotype; pulsed-field gel electrophoresis (PFGE); restriction endonuclease analysis (REA);
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Times Cited By KSCI : 4  (Citation Analysis)
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