• Asian-Australasian Journal of Animal Sciences
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• 제33권6호
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• pp.930-940
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• 2020

Objective: This study was conducted to evaluate changes in nutritional value and in situ dry matter (DM) degradability of oak and pine wood before and after steam-digestion process (60 min/160℃/6 atm) and feeding effect of the oak roughage on performance and behavior of Hanwoo steers. Methods: Chemical composition and tannin concentration were analyzed for oak and pine trees before and after the pretreatment. In situ DM and effective degradability of these samples were assessed using a nylon bag method. In vivo trial was performed to estimate animal performance and behavior, using steers fed total mixed ration (TMR) diets containing 0% (control), 25% (OR-25), and 50% (OR-50) of the oak roughage. Eighteen steers were allocated into nine pens (2 steers/pen, 3 pens/treatment) for 52 days according to body weight (BW) and age. Results: By the steam-digestion treatment, the neutral detergent-insoluble fiber was decreased from 86.5% to 71.5% for oak and from 92.4% to 80.5% for pine, thereby increasing non-fiber carbohydrate. In situ DM degradability of treated oak reached 38% at 72 h, whereas that of untreated oak was only 11.9%. The 0 h degradability of the treated pine increased from 5.9% to 12.1%, but the degradability was unchanged thereafter. Animal performance including BW, average daily gain, DM intake, and feed conversion ratio was not different among control and oak treatments. No differences were detected in animal behavior such as lying, standing, rumination, drinking, and eating, except walking. Walking was higher in control than oak treatments with numerically higher eating and lower lying times, probably due to bulkier characteristics of rice straw in the diet. Conclusion: This study demonstrates that the oak roughage can be substituted for 50% of total forage or 100% of rice straw in TMR diets at early fattening stage of Hanwoo steers.

• 한국축산식품학회지
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• 제35권6호
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• pp.765-771
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• 2015

The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B₁₂ content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B₁₂ was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B₁₂ detection. The calibration curve showed good linearity with the coefficient of correlation (r²) value of 0.9999. The limit of detection was 0.03 µg/L and the limit of quantitation was 0.1 µg/L. The method of detection limit was 0.02 µg/kg. The vitamin B₁₂ recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B₁₂ content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B₁₂. This method could be implemented in laboratories that require time and labor saving.

• 한국제4기학회지
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• 제17권2호
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• pp.163-163
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• 2003

The study area, Kwangneung Experiment Forest (KEF) is located on the west-central portion of Korean peninsula and belongs to a cool-temperate broadleaved forest zone. At the old-growth deciduous forest near Soribong-peak (533.1m) in KEF, we have established a permanent plot and a flux tower, and the site was registered as a KLTER site and also a KoFlux site. In this study, we aimed to present basic ecological characteristics and synthetic data of carbon budgets and flows, and some monitoring data which are essential for providing important parameters and validation data for the forest dynamics models or biogeochemical dynamics models to predict or interpolate spatially the changes in forest ecosystem structure and function. We made a stemmap of trees in 1 ha plot and analyzed forest stand structure and physical and chemical soil characteristics, and estimated carbon budgets by forest components (tree biomass, soils, litter and so on). Dominant tree species were Quercus serrata and Carpinus laxiflora, and accompanied by Q. aliena, Carpinus cordata, and so on. As a result of a field survey of the plot, density of the trees larger than 2cm in DBH was 1,473 trees per ha, total biomass 261.2 tons/ha, and basal area 28.0 m2/ha. Parent rock type is granite gneiss. Soil type is brown forest soil (alfisols in USDA system), and the depth is from 38 to 66cm. Soil texture is loam or sandy loam, and its pH was from 4.2 to 5.0 in the surface layer, and from 4.8 to 5.2 in the subsurface layer. Seasonal changes in LAI were measured by hemispherical photography at the l.2m height, and the maximum was 3.65. And the spatial distributions of volumetric soil moisture contents and LAIs of the plot were measured. Litterfall was collected in circular littertraps (collecting area： 0.25m2) and mass loss rates and nutrient release patterns in decomposing litter were estimated using the litterbag technique employing 30cm30cm nylon bags with l.5mm mesh size. Total annual litterfall was 5,627 kg/ha/year and leaf litter accounted for 61% of the litterfall. The leaf litter quantity was highest in Quercus serrata, followed by Carpinus laxiflora and C. cordata, etc. Mass loss from decomposing leaf litter was more rapid in C. laxiflora and C. cordata than in Q. serrata litter. About 77% of C. laxiflora and 84% of C. cordata litter disappeared, while about 48% in Q. serrata litter lost over two years. The carbon pool in living tree biomass including below ground biomass was 136 tons C/ha, and 5.6 tons C/ha is stored in the litter layer, and about 92.0 tons C/ha in the soil to the 30cm in depth. Totally more than about 233.6 tons C/ha was stored in DK site. And then we have drawn a schematic diagram of carbon budgets and flows in each compartment of the KEF site.

• 한국수산과학회지
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• 제32권3호
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• pp.338-344
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• 1999

Floating debris was recorded from a training ship, $\sharp$1 Kwanaksan, of Pukyong National University with about 10 knots speed at July 15th and 20th of 1997. The sampled area is the middle of southern sea of Korea, divided into 44 unit segments on survey routes. Debris fabrication materials were categorized with 6 items using the following; man-made or natural wood items, paper and cardboard, nylon netting and rope, styrofoam, plastics, floating metal and glass containers. All identified items within a 100 $\pm$ 2 m wide band were recorded but ignored if beyond this boundary. The results of distribution and transport of floating debris in the area are as follows: 1. The quantities of debris during the survey were distributed from $1.6\~369.7\;items/km^2$. The most obvious trend is the widespread distribution of all debris. The highest densities of all debris were discovered in the coastal waters of Namhae and Yokji island, and of about 50 km off from the southward of Yokji and about 74 km off from the eastward of Komun island. Especially many of small styrofoams within $\phi$20 cm were observed in these segments. 2. Styrofoams and plastics were composed of $83.5\%$ among all debris, next woods items, $9.8\%$. 3. The quantities, distribution shapes and composition of debris were varied as the observed duration and the natures of each items. 4. These phenomena are concluded that firstly they depend on the river discharges included debris due to precipitation falls, secondly inflow or dumping debris are drifting to the off-shore by Kuroshio currents present at their adjacent sea, But on the basis of the observed data it is difficult that source position, quantities and inflow items of debris are identified, and also the transport processes is pursue. further more surveys are continuously being investigated, and from this it is hoped that a much wider coverage can be achieved, perhaps on all sites of the Coast of Korea and contributed to the stationary area, finding of sources, removal method of debris and resistants of marine productivity.

• 한국산학기술학회논문지
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• 제18권3호
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• pp.527-533
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• 2017

의류 산업의 발전과 함께 다양한 염료를 이용한 염색 기술이 개발되어 왔다. 최근 합성색소의 인체에 대한 유해성으로 인하여 천연색소에 대한 관심이 증가하고 있으며, 이에 미생물 색소의 개발과 활용이 요구되고 있다. 본 연구에서는 해양세균 Microbulbifer sp. PPB12 균주가 생산하는 보라색 천연색소를 이용하여 다섬교직포에서 염색 효과와 생리활성을 평가하였다. 보라색 색소는 Microbulbifer sp. PPB12 균주를 Marine broth 2216을 이용하여 3일 동안 배양 후 에탄올을 이용하여 추출하였다. 20% 에탄올에 녹인 보라색 색소는 다섬교직포의 면, 아세테이트 특히 나일론에 우수한 염착력을 나타내었고, 최적의 염색조건은 염색온도 $80-90^{\circ}C$, 염색시간 1시간 이상, pH 4-6, 욕비 1:25로 나타났다. 매염처리는 $Na_2SO_4$를 사용하여 염색 10분 후 후매염 처리 하는 것이 색상 발현에 더 적합하였으나 매염제를 처리하지 않은 경우와 큰 차이를 보이지 않았다. 또한 보라색 색소는 B. subtilis에서 항균성을 나타내었다. 이러한 결과, 본 해양 세균 색소는 항균성이 부가된 천연 섬유 염색제로 이용 가능성이 확인되었다.

• 한국식품영양과학회지
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• 제38권12호
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• pp.1767-1772
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• 2009

절임배추를 각각 다른 5종류의 필름에 sealing 한 다음 4주간 4${^{\circ}C}$로 저장하면서 품질 특성을 평가하였다. 기체투과도 조절 필름인 MF필름(US patent No. 5972815)으로 포장한 절임배추의 경우에는 미생물의 성장수가 가장 적었고 HDPE필름으로 포장한 절임배추의 경우가 미생물수가 가장 많았다. MF필름으로 포장한 절임배추의 경우 다른 필름으로 포장한 절임배추보다 pH가 천천히 감소하였고 산도의 경우에는 모든 필름에서 포장한 절임배추에서 4주 동안 점점 증가하지만 MF필름으로 포장한 절임배추의 산도가 가장 낮았다. MF필름으로 포장한 절임배추의 경우 탄력성의 감소율이 가장 낮았고 HDPE필름으로 포장한 절임배추의 경우에 탄력성의 감소율이 가장 높았다. MF필름에 절임배추를 포장한 경우 포장 필름의 투과성과 절임배추의 호흡에 의해 4${^{\circ}C}$에서 1주 후 필름 안에 $O_2$ 조성이 0.66%로 나타났는데, 이러한 필름 안의 $O_2$ 조성 결과와 미생물수, pH, 산도의 결과를 비교해보면 포장필름 안에 $O_2$ 조성이 1%에 가까울 때 절임배추의 품질특성이 가장 우수하게 유지되었다. 본 연구 결과로 절임배추의 저장성 증진과 품질특성의 유지를 위한 필름으로 MF필름이 가장 적합하였다. 그리고 LDPE필름 역시 MF필름보다는 저장성 증진과 품질특성 유지 면에서 성능은 떨어졌지만 가격 면이나 대중적인 면에서 고려해 보았을 때 LDPE필름의 사용도 가능하다고 하겠다.

• Composites Research
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• 제29권6호
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• pp.408-413
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• 2016

흔히 사용되는 고분자는 다양한 형태로 제조되고 있다. 더 나아가, 나일론, 아라미드와 같은 연속적인 고분자 섬유가 최근에 제조되고 있다. 이번 실험에서 에폭시를 이용하여 고분자 섬유를 제조하였다. 비스페놀-A 타입의 에폭시가 사용되었고, 아민계 및 산 무수물계 경화제가 사용되었다. 에폭시 형상을 유지하기 위해 단계적 승온방식을 이용하여 에폭시 섬유를 경화했다. 계면접착력을 확인하기 위하여 두 섬유에 대한 상대적인 표면에너지를 수정된 정적 접촉각 방식으로 확인하였다. 인장실험을 통하여 기계적인 실험을 실시하였다. 인장실험 후에 파단형태가 경화제에 따라 달라지는 것을 확인하였다. 아민계 경화제를 사용한 에폭시 섬유의 경우 인장강도가 183 MPa로 70 MPa인 산 무수물계 경화제를 사용한 에폭시를 사용한 에폭시 섬유보다 더 좋은 것을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권6호
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• pp.673-679
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• 1998

The experiment was conducted to determine in situ rumen degradability of soybean meal (SM), fish meal (FM), sesame cake (SC) and Italian ryegrass hay (IRGH) and the effect of supplementing the above protein meals to IRGH on digestibility and balance of nutrients in three Saanen goats. For measuring the degradability, nylon bags containing each meal were incubated in the rumen of one fistulated dry cow for 3, 6, 12, 24, 36, and 48 hrs. Degradability revealed that SM protein was highly degradable in the rumen (99.1%), while FM protein was less degradable (76.8%) with SC protein being intermediate (91.2%) at 48 hrs of incubation (p < 0.01). Degradation rate of the potentially degradable fraction was estimated to be 12.12, 5.88 and 5.88%/hr for SM, FM and SC, respectively. In the metabolism trial, all goats were offered daily 900 g IRGH and one of the supplements, SM (100 g), FM (75 g) or SC (100 g). Intake of DM, OM, CP and GE was similar among diets. However, digestibility of OM for SC diet was significantly lower than that for diets supplemented with FM and SM (p < 0.10). Nitrogen (N) excreted in faeces (p < 0.05) and in urine (p < 0.10) was, respectively, higher and lower for SC diet than that for the other two diets. The same tendency was observed in energy losses in faeces (p < 0.10) and in urine (p < 0.05). There was no difference in energy loss in methane or in heat production among diets. Consequently, no significant difference was observed in N retention (2.13, 0.42 and -0.11 g/day for FM, SC and SM diet, respectively) or in energy retention (-1.49, -2.14 and -2.70 MJ/day for FM, SM and SC diet, respectively). These results showed that protein supplements affected the digestion of diets based on grass hay with 7.45% CP in DM in goats, although there was no significant influence on N or energy retention.

• 한국식품영양학회지
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• 제15권3호
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• pp.197-202
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• 2002

유통 시판 중인 육포류를 수집하여 외형 상태, 성분 배합 및 첨가물 이용 상태, 영양 조성분 및 위생적인 상태를 조사, 분석하여 품질수준을 평가하였다. 1. 원료는 수입우육 국내 제조품에서, 형태는 strips형이, sealing은 무진공 상태, 포장 재료는 nylon/polyethylene적층 필름이 많았으며, 돈육포류는 국내산 원료육으로만 제조되고 있었다. 2. 조미·배합물과 첨가물 사용은 우육포류에서 국내산 육포류 16종, 수입육 국내 제조육포류 23종, 수입육포류 22종이며, 돈육 육포류는 13종으로 나타내었다. 수분 함량은 수입 우육포류에서, 조단백질 함량은 수입 우육포류와 돈육포류에서, 조지방은 돈육포류에서 각각 높았으며 조회분은 육포류간에 유사한 함량 수준을 보였다. 3. 염도는 3차 측정 평균값으로서 수입 우육포류가 높았으며 국내산 우육포류가 낮았다. pH 범위는 5.41∼6.11 사이로 수입 우육포류가 약간 높고, 국내산 우육포류가 낮게 보였다, 수분활성도는 돈육포류가 평균 0.743으로 높고, 국내산 우육포류가 가장 낮게 나타내었다. 4. VBN값은 육포류에서 평균 9.98∼12.36mg%범위로서 국내산 돈육포류가 가장 낮았고, TBA값은 육포류에서 평균 0.239∼0.367mg/kg으로 수입육 국내제조 우육포류가 높게 나타내고 국내산 돈육 포류가 낮은 수준이었다.

• 한국식품영양학회지
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• 제15권3호
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• pp.167-202
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• 2002

유통 시판 중인 육포류를 수집하여 외형 상태, 성분 배합 및 첨가물 이용 상태, 영양 조성분 및 위생적인 상태를 조사, 분석하여 품질수준을 평가하였다. 1. 원료는 수입우육 국내 제조품에서, 형태는 strips형이, sealing은 무진공 상태, 포장 재료는 nylon/polyethylene적층 필름이 많았으며, 돈육포류는 국내산 원료육으로만 제조되고 있었다. 2. 조미·배합물과 첨가물 사용은 우육포류에서 국내산 육포류 16종, 수입육 국내 제조육포류 23종, 수입육포류 22종이며, 돈육 육포류는 13종으로 나타내었다. 수분 함량은 수입 우육포류에서, 조단백질 함량은 수입 우육포류와 돈육포류에서, 조지방은 돈육포류에서 각각 높았으며 조회분은 육포류간에 유사한 함량 수준을 보였다. 3. 염도는 3차 측정 평균값으로서 수입 우육포류가 높았으며 국내산 우육포류가 낮았다. pH 범위는 5.41∼6.11 사이로 수입 우육포류가 약간 높고, 국내산 우육포류가 낮게 보였다, 수분활성도는 돈육포류가 평균 0.743으로 높고, 국내산 우육포류가 가장 낮게 나타내었다. 4. VBN값은 육포류에서 평균 9.98∼12.36mg%범위로서 국내산 돈육포류가 가장 낮았고, TBA값은 육포류에서 평균 0.239∼0.367mg/kg으로 수입육 국내제조 우육포류가 높게 나타내고 국내산 돈육 포류가 낮은 수준이었다.