• 한국초지조사료학회지
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• 제34권1호
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• pp.45-51
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• 2014

본 연구는 버섯폐배지와 맥주박을 이용한 섬유질 발효사료의 급여효과를 알아보기 위해 흑염소의 생산성과 영양소 이용율을 조사하였다. 증체조사를 위한 사양 시험은 거세흑염소 20두를 이용하여 처리구당 5두씩 30일간 수행하였고, 소화율 시험은 거세흑염소 12두를 공시하여 처리구당 3두씩 라틴방각법으로 실시하여, 처리구는 대조구(배합사료 및 볏짚 급여구)와 3개의 시험구(버섯폐배지, 맥주박 비율이 각각 15와 25%(T1), 10과 30%(T2) 및 5와 35%(T3))로 각각 배치하였다. 일당증체량은 대조구와 T1구가 T3구 보다 유의하게 높게 나타났다(p<0.05). 1일 두당 건물섭취량과 유기물섭취량은 T1과 T2구가 대조구 보다 유의하게 높은 섭취량을 보였다(p<0.05). 건물소화율과 유기물소화율은 대조구와 T1구가 각각 62.39∼63.39%와 65.02~65.25%로 T3구의 각각 56.39와 59.01% 보다 유의하게 높게 나타났다(p<0.05). 질소축적량은 T1구가 가장 높게 나타났다(p<0.05). 따라서, 본 연구의 결과 버섯폐배지와 맥주박을 이용한 섬유질 발효사료는 시판사료를 급여한 대조구와 대등한 생산성과 영양소 이용율을 보였다. 그러나 버섯폐배지의 첨가 비율이 높아질수록 흑염소의 생산성 저하가 우려되므로 버섯폐배지 첨가는 25~30% 수준으로 배합하는 것이 필요하다 판단된다.

• 대한영양사협회학술지
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• 제5권1호
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• pp.100-115
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• 1999

With the growing number of nutrient calculation software packages on the market, there are need to compare each programs. Since each program use different nutrient databases, the result of calculation may be different in value. In this study, we use three(A, B, C) most popular program package to compare the result of nutrient calculation. For the analysis, 24hour recall data from 97 preschool children, 66 university students and 95 aged persons were used. For the calculation if subjects gave the complete recipe, recipes from the subjects were used. Otherwise, recipe from the program database were used. Common 15 nutrients of which all program can give results, are analyzed and compared for mean nutrient intake and nutrient intake for food groups. Ten nutrients among 15 nutrients which have RDA were analyzed for % of RDA and the distribution of RDA. Mean nutrient intake of Fe, vitamin A, Na were statistically different among results of the calculation using three programs(p<0.001). The distribution of Fe, vitamin A and vitamin $B_2$, niacin were statistically different among three results of the calculation using three program(p<0.001, p<0.05). Nutrient intakes of food groups were statistically different in cereal and products, bean and products, vegetables, fruits, fishes and shellfishes, milk and products, beverages, and seasonings(p<0.0001). It is hard to say that the difference among three program are coming from the difference from nutrient database or recipe database in this study. With these result, we conclude that it is necessary to evaluate nutrient database and recipe database as the foremost consideration in selecting nutrient calculation software. Those differences should be considered when interpreting results, comparing results with other studies, and when developing treatment plans in the clinical settings.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1598-1604
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• 2001

Eight $12.4{\pm}0.6kg$ initial body weight crossbred barrows were used to determine the effect of soybean galactooligosaccharides on nutrient and energy digestibility, and digesta transit time. Four dietary treatments were utilized in this trial. Treatment one was a corn-soybean meal based diet (SBM) containing raffinose and stachyose at the levels of 0.16% and 0.75%, respectively. Treatment two (control) was a corn-HP300 (soybean concentrate protein) diet. In treatments three and four, 1.1% and 2.2% commercial stachyose was added to the control diet to provide total dietary stachyose at the levels of 1% and 2%, respectively. The soybean galactooligosaccharides (raffinose + stachyose) level in treatment one was slightly lower compared to that in treatment three. Three collection periods were run with two pigs for each treatment/period. There was a 4 d adjustment period followed by a 3 d collection period. The results showed that the nitrogen retention (86.79%) of pigs fed treatment two diet was higher than that of pigs fed treatment one by 5.2% (p<0.05). The nitrogen retention of treatment three was intermediate 83.09%. The apparent fecal digestibility of all amino acids in treatment two was numerically highest, followed by treatments three and four. However, there were no significant difference among groups (p>0.05). The dry matter (DM), organic matter (OM), crude protein (CP), and crude fiber (CF) digestibility numerically decreased as the soybean galactooligosaccharides level increased, but were not significantly different (p>0.05). Chromium content in feces (from the inclusion of 0.3% chromic oxide in the diets) differed among treatments (p<0.05) at 15 h, 18 h, and 21 h after eating. This showed that the digesta transit time was differed significantly among treatments. Treatment four was the shortest, followed by treatment three, SBM and control. The results demonstrated that in the absence of antinutritional factors and soybean antigen protein, inclusion of 1% and 2% stachyose in corn-HP300 diet has no significant effect on the digestibility of DM, OM, CP, CF and amino acids. When the soybean galactooligosaccharide level in diet one and diet three were adjusted to be almost the same, antinutritional factors such as trypsin inhibitor and soybean antigen protein could decrease the nutrient digestibility and nitrogen retention rate of diet. High levels of soybean galactooligosaccharides shortened the digesta transit time in the intestinal tract. This trial suggested that the total level of soybean galactooligosaccharides (stachyose+raffinose) in the weanling piglet diet is better not to exceed 1% when common soybean meal is used as main protein source.

• 한국산림과학회지
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• 제88권2호
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• pp.249-254
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• 1999

여러 가지 Al농도가 소나무 묘목의 생장, 영양상태 및 광합성속도에 미치는 영향을 수경재배법에 의하여 조사하였다. 배양액의 Al농도는 0(대조구), 10, 30 및 60ppm의 4처리구로서 1/5000a 포트에 배양액의 pH를 4.0으로 조절한 후, 소나무(Pinus densiflora Sieb. et Zucc.) 2년생 묘목을 배양액에 이식하여 1996년 5월 8일부터 8월 6일까지 90일 동안 온실에서 생육시켰다. 소나무 묘목은 10ppm 이상의 처리구에서 건물생장의 감소를 초래하였다. 소나무 묘목의 상대생장율(RGR) 및 순동화율(NAR)은 배양액의 Al농도가 증가함에 따라서 낮아지는 것으로 나타났다. 또한 Al농도가 증가함에 따라서 순광합성속도도 저하되었다. 이는 Al처리에 의한 소나무 묘목의 RGR 및 NAR저하는 광합성저해가 원인이라고 생각된다. 또한, 60ppm 처리구에서 잎의 Ca 및 Mg농도는 대조구 및 10ppm 처리구에 비하여 감소하였으며 식물체 각 기관의 Al농도는 10ppm 이상의 처리구에서 대조구에 비하여 증가하였다. 이것은 지하부에 축적된 Al에 의해서 뿌리의 생리기능이 저하되어, 양분흡수 능력이 떨어져 결과적으로 지상부의 Ca 및 Mg와 같은 식물생육 필수원소의 농도가 감소되었음을 시사하고 있다.

• 한국물환경학회지
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• 제26권5호
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• pp.781-788
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• 2010

A pilot study was performed to examine the feasibility of multiple stage of constructed wetland (CW) for nutrient removal. The system is composed of six wetland cells connected with water-ways. The hydraulic of wetland cells is designed as free water surface flow. The treatment capacity was $25m^3d^{-1}$ at HRT of about one day for each cell. The magnitude of nutrient removal was related with the length of wetlands and plant density. Total N and P removal rates were 1353 and $246mg\;m^{-2}d^{-1}$ respectively. The pilot-scale reactor was model as continuous flow system containing contribution of CSTR and PFR typed-reactors. The $k-C^*$ model equation was applied to predict N and P reduction. The result indicated the equation was well guided to estimate reduction of $NO_3-N$ and $PO_4-P$.

• 생명과학회지
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• 제11권3호
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• pp.197-202
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• 2001

This study was conducted to investigate the effect of simulated acid solution(SAS) on acid buffering capacity, chlorophyll content and butrient leacking in 4 herb species(Petunia hybrida Vilm, Gomphrena globosa L. Celosia cristat L. Salvia officinallis L) . The acid buffering capacity in the leves was increased in the treatment of pH 3.0 in Celosia L., whereas it was increased at pH 4.0 in Petunia Petunia hybrida Vilm. and Gomprean globosa L.. But, the acid buffering capacity of the leaves did not work at ph 2.0 treatment in 4 herb species. With decreasing pH level, the chlorophyll content of Petunia hybrida Vilm. and Gomphrena globosa L. Was markedly decreased than that of Gelosia cristata L. and Savia officinalis L. As the pH levels decreased from 5.6 to 2.0 the nutrient leaching from leaves was significantly increased in 4 herb species. In pH 4.0 and 5.6, the concentrations of nutrient leaching from leaves were higher in Perunia hybrida Vilm. and Gomphrean globosa L. than Gelosia cristata L. and Salvia officinalis L., Based on the results, there was a great differences in response to SAS among the 4 herb species. Im general, Gelosia cristata L. and Salvia officinalis L. represented a higher tolerance to SAS Petunia hybrida Vilm, and Gomphrena globosa L..

• Asian-Australasian Journal of Animal Sciences
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• 제26권12호
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• pp.1689-1697
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• 2013

This study was designed to determine the effect of physical form and urea treatment of rice straw on rumen fermentation, microbial protein synthesis and nutrient digestibility. Four rumen-fistulated dairy steers were randomly assigned according to a 2 (2 factorial arrangement in a 4 (4 Latin square design to receive four dietary treatments. Factor A was roughage source: untreated rice straw (RS) and urea-treated (3%) rice straw (UTRS), and factor B was type of physical form of rice straw: long form rice straw (LFR) and chopped (4 cm) rice straw (CHR). The steers were offered the concentrate at 0.5% body weight (BW) /d and rice straw was fed ad libitum. DM intake and nutrient digestibility were increased (p<0.05) by urea treatment. Ruminal pH were decreased (p<0.05) in UTRS fed group, while ruminal ammonia nitrogen ($NH_3$-N) and blood urea nitrogen (BUN) were increased (p<0.01) by urea treatment. Total volatile fatty acid (VFA) concentrations increased (p<0.01) when steers were fed UTRS. Furthermore, VFA concentrations were not altered by treatments (p>0.05), except propionic acid (C3) was increased (p<0.05) in UTRS fed group. Nitrogen (N) balance was affected by urea treatment (p<0.05). Microbial protein synthesis (MCP) synthesis were greater by UTRS and CHR group (p<0.05). The efficiency of microbial N synthesis was greater for UTRS than for RS (p<0.05). From these results, it can be concluded that using the long form combined with urea treatment of rice straw improved feed intake, digestibility, rumen fermentation and efficiency of microbial N synthesis in crossbred dairy steers.

• Journal of Microbiology
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• 제42권1호
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• pp.1-8
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• 2004

Changes in the bacterial populations of a 5-stage biological nutrient removal (BNR) process, with a step feed system for wastewater treatment, were monitored by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA fragments. DGGE analysis indicated seasonal community changes were observed, however, community profiles of the total bacteria of each reactor showed only minor differences in the samples obtained from the same season. The number of major bands was higher in the summer samples, and decreased during the winter period, indicating that the microbial community structure became simpler at low temperatures. Since the nitrogen and phosphate removal efficiencies were highly maintained throughout the winter operation period, the bacteria which still remaining in the winter sample can be considered important, playing a key role in the present 5-stage BNR sludge. The prominent DGGE bands were excised, and sequenced to gain insight into the identities of the predominant bacterial populations present, and most were found to not be closely related to previously characterized bacteria. These data suggest the importance of culture-independent methods for the quality control of wastewater treatment.

• 한국환경복원기술학회지
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• 제11권5호
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• pp.1-11
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• 2008

This study was carried out to provide primary data set for cleaning of contamination site. By having hydroponic culture on Salix alba and S. caprea seedling treated with Ni solution, the result of Ni accumulation came out as followings : In a treatment of Ni to the Salix alba and S. caprea, Ni accumulation increased in its root, leaves, and stem, as Ni concentration became higher until $10.0{\mu}mol$. But in a $100.0{\mu}mol$ treatment, the seedlings died after 4 weeks. Ni accumulation in the Salix alba and S. caprea was the highest in its roots, second-highest in leaves, and the lowest in stems. In the case of $10.0{\mu}mol$ treatment of Ni solution, Ni accumulation in roots were above 500.0mg/L, and leaves were above 20.0mg/L. But it was lower than 13.0mg/L in stems. Ni accumulation in the plant increased more when nutrient solution containing Ni was weekly changed than just refilling the same amount of nutrient solution that evaporated Ni accumulation in Salix alba was higher than S. caprea when the nutrient solution had been refilled only.

• 농업과학연구
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• 제47권4호
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• pp.859-865
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• 2020

A total of 100 growing pigs (25.66 ± 1.55 kg) were used in a 16-week feeding trial. Pigs were randomly distributed into two treatment groups on the basis of body weight and sex. There were ten replicate pens per treatment, with five pigs (three barrows and two gilts) per pen. The dietary treatments for this trial were as follows: 1) Basal diet (CON) and 2) T1 (γ-aminobutyric acid, GABA), CON + 100 mg·kg-1 GABA. During weeks 0 to 6, the feed efficiency (G : F) in the T1 group was higher (p < 0.05) than that in the CON group, but no improvements in the average daily feed intake (ADFI) and average daily gain (ADG) were noted (p > 0.05). During the experimental period, the final body weight (BW) was improved (p < 0.05). However, no differences (p > 0.05) were noted in the apparent total tract digestibility (ATTD) of dry matter (DM) nitrogen (N). Meanwhile, there were no significant differences (p > 0.05) observed in the backfat thickness among the treatment groups. These results therefore indicate that supplementation with GABA may improve the overall growth performance but may not lead to differences in the nutrient digestibility and backfat thickness in growing-finishing pigs.