• Preventive Nutrition and Food Science
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• v.8 no.4
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• pp.390-394
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• 2003

In the post-genome period, the technique for identifying gene expression has been progressed to high throughput screening. In the field of molecular nutrition, the use of screening techniques to clarify molecular function of specific nutrients would be very advantageous. In this study, we have evaluated Zn-regulated gene expression in Zn-deficient, homocystein-treated EA.hy926 cells, using cDNA microarray, which can be used to screen the expression of many genes simultaneously. The information obtained can be used for preliminary assessment of molecular and signaling events modulated by Zn under pro-atherogenic conditions. EA.hy926 cells derived from human umbilical vein endothelial cells were cultured in Zn-adequate (control, 15 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) Dulbecco's MEM media under high homocysteine level (100 $\mu$M) for 3 days of post-confluency. Cells were harvested and RNA was extracted. Total RNA was reverse-transcribed and the synthesized cDNA was labeled with Cy3 or Cy5. Fluorescent labeled cDNA probe was applied to microarray slides for hybridization, and the slide was then scanned using a fluorescence scanner. The expression of seven genes was found to be significantly decreased, and one significantly increased, in response to treatment of EA.hy926 cells with Zn-deficient medium, compared with Zn-supplemented medium. The upregulated genes were oncogenes and tumor suppressor genes, cell cycle-related genes and transporter genes. The down-regulated gene was RelB, a component of the NF-kappaB complex of transcription factors. The results of this study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, namely Zn. Furthur study, using tailored-cDNA array and vascular endothelial cell lines, would be beneficial to clarify the molecular function of Zn in atherosclerosis, more in detail.

• Archives of Pharmacal Research
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• v.27 no.1
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• pp.99-105
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• 2004

Isoflavones have been a central subject in research on the natural phytoestrogens found in Leguminosae. Their effects on bone formation and remodeling are important in that they can act like estrogen by binding on estrogen receptors on the target cell surface. We, therefore, believed that isoflavones may help in the treatment of patients with estrogen deficiency disease such as estrogen replacement therapy (ERT) for osteoporosis. As commonly known, osteoporosis is one of the hormonal deficiency diseases, especially in menopausal women. When estrogen is no longer produced in the body a remarkable bone remodeling process occurs, and the associated events are regulated by growth factors in the osteoblast lineage. In the present study, we investigated whether isoflavones (Isocal) extracted from Sophorae fructus affect the growth factors IGF-I and TGF-$\beta$ that have been known to be related with bone formation. In the study, we found that the active control (PIII) effectively enhanced the level of nitric oxide (NO) and growth factors, and thereby inhibited osteoclastogenesis. The most efficient concentration was $10^{-8}$% within five days, whereas the comparative control (soybean isoflavone) was not as effective even at a lower concentration. In conclusion, the products which contain enriched glucosidic isoflavone and nutrient supplements such as shark cartilage and calcium can be used for osteoporosis therapy by enhancing the production of IGF-I and TGF-$\beta$. Furthermore, the NO produced through endothelial constitutive NO synthase (ecNOS) may playa role in inhibiting bone reabsorption.

• Journal of Korean Home Economics Education Association
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• v.20 no.2
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• pp.75-94
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• 2008

The purposes of this paper are to describe the newly established reference values of nutrient intakes: to apply the changed dietary reference intakes to the new text book based on the revised curriculum: and to contrive substantial contents in the domain of dietary life(foods & nutrition) of new text book. Dietary Reference Intakes for Koreans(KDRIs) is newly established reference values of nutrient intakes that are considered necessary to maintain the health of Koreans at the optimal state and to prevent chronic diseases and overnutrition. Unlike previously used Recommended Dietary Allowances for Koreas(KRDA), which presented a single reference value for intake of each nutrient, multiple values are set at levels for nutrients to reduce risk of chronic diseases and toxicity as well as prevention of nutrient deficiency. The new KDRIs include the Estimated Average Requirement(EAR), Recommended Intake(RI), Adequate Intake(AI), and Tolerable Upper Intake Level(UL). The EAR is the daily nutrient intake estimated to meet the requirement of the half of the apparently healthy individuals in a target group and thus is set at the median of the distribution of requirements. The RI is set at two standard deviations above the EAR. The AI is established for nutrients for which existing body of knowledge are inadequate to establish the EAR and RI. The UL is the highest level of daily nutrient intake which is not likely to cause adverse effects for the human health. Age and gender subgroups are established in consideration of physiological characteristics and developmental stages: infancy, toddler, childhood, adolescence, adulthood and old age. Pregnancy and lactation periods were considered separately and gender is divided after early childhood. Reference heights and weights are from the Korean Agency for Technology and Standards, Ministry of Commerce, Industry and Energy. The practical application of DRIs to the new books based on the revision in the 7th curriculum is to assess the dietary and nutrient intake as well as to plan a meal. It can be utilized to set an appropriate nutrient goal for the diet as usually eaten and to develop a plan that the individual will consume using a nutrient based food guidance system in the new books based on the revision in the 7th curriculum.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.399-403
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• 2000

Recently, abnormal leaf chlorosis has been observed in jujube trees of the major production area in Kyongbuk province. To determine possible causes for the observation, physico-chemical properties of the orchard soil and nutrient contents in the jujube trees were investigated. The chlorosis symptoms were categorized into three types based on the site of chlorosis initiation; leaf center, petiole, and intravein. Of the three, leaf center chlorosis was the most common. The symptom occurred more frequently in Ihyeon, Gyuam, and Geugrag soil series with previous history of apple orchard. In general, the soil and the tree showing leaf chlorosis had lesser nutrient contents than normal except P, Fe, and Zn. In particular, Mg contents in leaf, branchlet, and fruit of the tree were as much as 4 times lesser than those in normal tree; the soil also had 7.8 times lesser Mg contents. The results indicate that the abnormal leaf chlorosis in jujube tree may be caused by nutrient deficiency in soil and could be cured by proper fertilization.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.373-386
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• 2017

The study was conducted to investigate seasonal nutrient dynamics in rice-cultivated soils collected from farmhouses of three large-scale environment-friendly agricultural districts (LEAD), Jangheung, Suncheon, and Okcheon in which environmental-friendly agriculture has been exemplarily practiced in Korea. Among three districts, Crop- livestock cycling organic farming system had been introduced only in Jangheung. pH and EC of farmhouse soils of three LEADs were ranged between 5.5 - 6.7 and $0.4-1.0dS\;m^{-1}$ from March to September, respectively. T-N was observed to be high on the farmhouse soil in Suncheon and K was observed to be lowest on farmhouse soil in Okcheon. Concentration of $NH_4-N$ in soil was observed to be highest on June, in particular on the farmhouse in Jangheung, but rapidly decreased due to the loss of fertilizer applied in Spring. Yield and harvest index were the highest on the farmhouse in Okcheon in which total annual gross production $ha^{-1}$ was nearly three time higher than those of other two farmhouses. Farmhouse soil of Okcheon was maintained the highest seasonal nutrient balance due to the high input of fertilizer. It was estimated that K balance in the farmhouse soil in Suncheon dropped to $-60kg\;ha^{-1}$ on September, and it might have some effect on the less rice productivity due to K deficiency. Farmhouse soil in Jangheung was maintained low seasonal balance of T-N and P but showed the highest N use efficiency in the rice grain. Based on above-mentioned results, we think Jangheung farmhouse can be recommended as a model farmhouse of LEADs.

• Journal of Environmental Science International
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• v.26 no.11
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• pp.1255-1266
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• 2017

Phosphorus (P) is an essential and major nutrient for both plants and animals. However, anthropogenic P in the environment may cause severe problems such as the deterioration of water quality. Therefore, it is essential for the Korean government to manage P in the agricultural sector. The annual P budget for Korea was 46 kg P ha-1 in 2013, placing Korea in second among Organisation for Economic Co-operation and Development (OECD) countries. P surplus and deficiency in agricultural lands can be estimated according to the P budget, which is one of the OECD agri-environment indicators. In the P budget, it is important to ensure consistency in the input-output data sources, in order to apply national and regional policies for the environmentally sound management of agricultural P. This study examines the impacts on the input-output data sources in the regional P budget in Korea. P budgets were between 99-145 kg-P/ha, depending on different data sources. We suggest two recommended data combinations (DC 1 and DC 2) for reliability of the data. P budgets calculated using DC 1 and DC 2 were 128 kg-P/ha and 97 kg-P/ha, respectively. According to the results, one of the core factors affecting P budgets was crop production. In this study, DC 2 was recommended rather than DC 1 in order to consider the cultivated areas for various crops. It is also necessary to analyze the sensitivity of the coefficients used in P budget in the future.

• Journal of Nutrition and Health
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• v.37 no.10
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• pp.899-907
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• 2004

This study was performed to investigate dietary habits and nutritional intake according to body mass index (BMI) of female college students. The subjects were 1361 students with a mean age of 20.4 years residing in Seoul and Kyonggui area. BMI (kg/) between $\geq$ 18.5 to $\leq$ 23 was considered as nonnal, < 18.5 as underweight and > 23 as overweight. The subjects had a mean BMI of 20.0 kg/$m^2$ and 25% of them were classified as underweight group, 67% as normal group and 8% as overweight group. Dietary intake was assessed by food frequency method and consumption of foods and nutrients was analyzed. Frequency of meal skipping was higher in the overweight group, 16.1 % of the overweight group skipped dinner. Also, the frequency of snack intake was lowest in the overweight group. The overweight group consumed less meat and fish than the normal and underweight groups. The total number of foods consumed in a day was also significantly lower than that of the underweight and normal group. The overweight group consumed less energy, protein, fat, phosphorus and niacin than the underweight and normal groups. The overweight group took iron and sodium intake lower than the normal group. Mean intakes of nutrients except clacium and iron in all three groups were over Korea RDA. The NAR (nutrient adequacy ratio) value of all nutrients, except calcium, was lower in the overweight group when compared to the normal and underweight groups, and the MAR (mean adequacy ratio) of the overweight group was 0.89, which was lower than 0.92 of the underweight and 0.93 of the normal group. The above results indicated that the deficiency of major nutrients such as calcium and iron could be inducde by less kinds of consumed foods and frequent meal skipping including dinner in the overweighted group. Therefore, balanced nutrient intake is required to maintain skeletal health and prevent anemia in overweighted female college students aged twenties.

• Korean Journal of Community Nutrition
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• v.3 no.2
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• pp.228-244
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• 1998

The nutritional status of 362 elderly men and women in Chung-bud area was evaluated in terms of their nutrient intakes, biochemical and anthrophometric measurements by interviews with questionnaires from August to October 1996. Mean intake of all nutrients except ascorbic acid did not meet the RDA for this sample. Protein, vit A, reboflavin, calcium were the most likely to be deficient on the basis of propotions of elderly consuming less than 75% of the RDA. The subjects nutrient intake was significantly affected by gender, marital status, number of family, family composition, educational level, pocket money, and region. Men in rural areas and women over 75 in urban areas were the most vulnerable groups with nutritional deficiency. According to serum biochemical indices, mean level of cholesterol, triglycerides, LDL, total protein, albumin and iron belonged to normal range but mean level of HDL showed below the normal range. More elderly men and women in urban areas showed a higher percentage of abormal level of cholesterol, triglycerides, LDL and HDL than those in rural areas. More elderly men and women in rural areas had abnormal levels of RBC, Hematocrit and hemoglobin compared to those in urban areas. Mean height and weight of elderly men was 161.4cm and 56.2Kg, respectively and 149.1cm and 50.5kg for women. The elderly in rural areas were taller than those in urban areas but had less weight, MAC, TSF, MAMC. Mean BMI of this sample belonged to normal range. However, the elderly in rural areas had a higher rate of underweight and lower for overweight than those in urban areas. The elderly in urban areas had higher blood pressure than those in rural areas.

• Journal of Nutrition and Health
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• v.35 no.9
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• pp.970-981
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• 2002

To assess riboflavin status by dietary intake and urinary excretion of preschool children in Busan and to evaluate the relationship of intakes of food and nutrient with urinary riboflavin excretion, riboflavin food frequencies of 40 common foods affecting intakes of riboflavin by food fequency method, nutrient intake by 24hr recall and 24hr urinary riboflavin excretion were measured with 97 preschool children. The mean riboflavin intake was 0.90 mg and above RDA. Dairy group was the primary source of riboflavin intake and provided 44.8% of the total daily riboflavin intake. The mean urinary riboflavin excretion and riboflavin excretion per gram of creatinine were 395.21$\mu\textrm{g}$ and 2110.41$\mu\textrm{g}$ respectively, The mean riboflavin intake (p<0.01, p<0.01), riboflavin density (p <0.001, p<0.001) and urinary riboflavin excretion per gram of creatinine (p<0.05, p<0.05) were significantly low with the two patterns of food group intake where dairy group was omitted (GMVFDS = 111101, consuming no dairy group and GMVFDS=l11001, consuming no fruit and dairy groups). On the basis of urinary riboflavin excretion per gram of creatinine, 14.3% of subjects in the group aged 1 to 3 and 18.2% of subjects in the group aged 4 to 6 were at risk of deficiency respectively. The urinary riboflavin excretion per gram of creatinine showed positive significant correlations with usual intakes of riboflavin from food groups of dairy (p<0.05), meat (p<0.05) and animal (p<0.05). So nutritional education is needed in order to consume dairy food group daily and to increase usual intake of animal food group including meat.(Korean J Nutrition 35(9) : 970~981, 2002)

• Journal of Nutrition and Health
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• v.3 no.1
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• pp.1-43
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• 1970

There is no any reports on nation-wide nutrition survey since 1947 in Korea, During the period only the small scaled nutrition surveys have been reported by several investigators. Authors, under the financial support from the Ministry of Health and Social Affairs, Republic of Korea, had conducted a nation-wide nutrition survey, including dietary, clinical, and socio-economical aspects, during the period from 29th of July to 15th of August, 1969. Number of households surveyed and population distribution are shown in Table II-2 and age and sex distribution of the population surveyed are also in Table II-3 in this report. Average food and nutrient intakes are shown in Tables II-6, II-7 and II-8 respectively. For the detailed discussions on food and nutrient intakes, see the Tables II-9 through II-11 and Figures II-1 through II-10 in this report. Anthropometric data are shown in Tables III-1 through III-4 and in Figures III-1 through III-4. Data of deficiency diseases are shown in Table III-5 and in Figure III-5 in this report. Socio-economical data are shown in Tables IV-1 through IV-26 and in Figures IV-1 through IV-3 in this report.