• Journal of Nutrition and Health
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• 제6권2호
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• pp.1-16
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• 1973

Some effects of dietary conditions on the metabolism of nucleic acid and protein in organs of the Albino Rats have been studied. The young rats to be examined were fed on the control diet and the diets deprived of one component among protein, carbohydrate, and lipid, such as protein free diet (PF: 432 kcal/100g) carbohydrate free diet (CF: 432kcal/100g), and lipid free diet (LF: 392kcal/100g) for three, seven, and fifteen days, respectively. The contents of DNA and RNA in the liver and the brain, and also those of protein-nitrogen(PN) and nonprotein-nitrogen (NPN) in the live, the brain, and the serum have been measured. The results are as followe: 1. The contents of DNA per gram of liver were increased by feeding on protein free diet. It is concluded that the critical factor for the result is not the increase in the rates of DNA syntheses, but the decrease in the turnover rates of DNA. 2. The metabolism of DNA in the liver showed the normal status by feeding on carbohydrate free diet. On the other hand, the rates of DNA syntheses were increased by feeding on lipid free diet. 3. The rates of DNA syntheses in the brain were decreased by feeding on the unbalanced diet, such as protein free, carbohydrete free, and lipid free diet. 4. In the liver and the brain, the rates of DNA syntheses were decreased by feeding on protein free diet. But the rates showed the normal status by feeding on the carbohydrate free diet, and also showed the similar metabolism to that in the case of the control group by feeding on lipid free diet. 5. In the liver, the rates of protein syntheses were decreased, whereas the contents of nonprotein-nitrogen were increased by feeding on protein free diet. 6. In the liver and the brain, the protein syntheses showed the more increasing rates than the rates in the case of the control diet by feeding on lipid free diet. 7. In the serum, the contents of protein did not change in a short period, also the insufficient feeling on protein was examined. It is clear that in the liver the rates of protein syntheses are decreased and the rates of protein catabolism are increased, since the rates of nucleic acid syntheses are decreased by feeding on the protein free diet. On the other hand, it is considered that in the brain the turnover rates of protein does not have correlation with the rates of nucleic acid syntheses, also these are decreased by feeding on protein free diet. And also it is believed that the phenomena of homeostasis for carrying the normal metabolism of nucleic acid and protein in the liver and the brain are operated in a short period as possible, by feeding on carbohydrate free and lipid free diets.

• Journal of Animal Science and Technology
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• 제63권1호
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• pp.160-169
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• 2021

Boiled feed is obtained by mixing and boiling agricultural by-products such as rice straw, rice bran, and bean curd with grains. The study explored the change in fatty acid, free amino acid, nucleotide, mineral, cholesterol, myoglobin and collagen of longissimus dorsi muscle in Hanwoo steers fed with boiled feed. Forty steers, 20 heads per group, were divided into two groups: a control group and a boiled feed group. The steers were raised for 10 months. The boiled feed group was enriched with palmitoleic acid, oleic acid, arachidonic acid and unsaturated fatty acids compared with the control group. There were no significant differences in amino acid and nucleic acid composition between the two groups. The boiled feed group contained higher levels of iron and manganese in the boiled feed group compared with the control group. The total cholesterol level was significantly increased, whereas calorie levels, myoglobin and collagen composition showed no differences. As the supply of boiled feed increases the content of fatty acids, unsaturated fatty acids and minerals related to flavor, it should be a feed that leads to the production of high-quality beef.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2004년도 International Conference Global Environmental Problems and their Health Consequences
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• pp.21-30
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• 2004

• 한국미생물·생명공학회지
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• 제2권2호
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• pp.119-119
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• 1974

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.312.2-312.2
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• 2002

The metal-ligand complex, ［Ru(phen)$_2$(dppz)]^{2＋}$ (phen = 1.10-phenanthroline, dppz = dipyrido［3.2-a:2', 3'-c］phenazine) (RuPD), was used as a spectroscopic probe for studying nucleic acid dynamics. The RuPD complex displays a long lifetime and a molecular light switch property upon DNA binding due to shielding of its dppz ligand from water. (omitted)

• 미생물학회지
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• 제13권3호
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• pp.101-108
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• 1975

Physiological effects of 2,4-D on the growth of Chlorella ellipsoidea were investigated culturing the alage in the MN4 media containing 0. $10^{-4}/M$ and $4<\times}10^{-4}M$ 2,4-D. During 6 days culture were taken to analysis with respect to overall growth, photosynthesis, respiration and chemical composition. Results obtained from the experiment were as follows : 1) The growth of chlorella was increased at $10^{-4}M$ and decreased at $4{\times}10^{-4}M$ of 2,4-D concentrations 2) At $10^{-4}M$ pf 2,4-D cpncentration, the activity of photosynthesis enhanced relative to contro. while at $4{\times}10^{-4}M$ it was not changed. In both concentrations, however, the rate of respiration was down from the control. 3) At $10^{-4}M$ 2,4-D, the concentration of carbondrate metabolites was not changed relative to control, while significant increase in the concentrations of proteins and nucleic acids was observed. On the other hand at $4{\times}10^{-4}M$ of 2,4-D concentrations, all the metabolites including carbohydrates, proteins and nucleic acids were descreased. 4) It is concluded that 2,4-D at $10^{-4}M$ concentration accelerates the growth of chlorella by promoting the activities of photosynthesis and biosynthesis of proteins and nucleic acids.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3657-3661
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• 2012

HBV infection is contagious and may be transmitted vertically or horizontally by blood products and body secretions. Over 50% of Iranian carriers have contracted the infection prenatally, making this the most likely route of transmission of HBV in Iran. This study assesses the resistance to Lamivudine in patients with chronic hepatitis B infection using a new ZNA probe Real Time PCR method. To evaluate the effectiveness of Lamivudine therapy for chronic hepatitis B infection, a study was conducted on 70 patients (63 men and 7women), who had received the drug first line. All patients were tested for the presence of HBsAg and HBeAg, the serum ALT level and the HBV DNA load before and after treatment. In all samples resistance to Lamivudine was tested with the ZNA Probe. Our results showed that ZNA Probe Real Time PCR method could detect wild type,YMDD, and its mutants, tyrosine-isoleucine-aspartate-aspartate and tyrosine-valine-aspartate-Aspartate. Among an estimated seventy patients with chronic hepatitis B infection, 18 (25.7%) were resistant to lamivudine. Only one patient was negative for presence of HBS-Ag (5.6%) and two patients were negative for HBe-Ag (11.1%). Real-time PCR with Zip nucleic acid probes is a sensitive, specific and rapid detection method for mutations in the YMDD motif, which will be essential for monitoring patients undergoing Lamivudine antiviral therapy.

• 생명과학회지
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• 제11권6호
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• pp.517-522
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• 2001

소립검정콩 청국장의 품질개선관 불쾌취 억압을 목적으로 키위와 무를 첨가하여 42$^{\circ}C$에서 3일간 발효시킨 소립 검정콩 청국장의 몇 가지 맛성분을 조사하였다. 소립 검정콩 청국장의 황색대두 청국장보다 유리아미노산 함량이 적었지만, 키위와 무를 첨가하여 발효시키면 유리아미노산의 함량이 증가되어 콩단백질 분해에 효과적이었다. 유기산은 모든 청국장에서 citric acid가 가장 많았으며, 다음올 acetic acid, lactic acid 순으로 많았다. 지방산은 linoleic acid (50.82~54.51%)> oleic acid(17.76~22.10%)> plamitic acid(12.13~13.79%) 순으로 많았다. 청국장의 종류별로 유기산과지방산 비율은 유의적인 차이가 없었다. 청국장의 주요 휘발성 성분으로는 indole, 2,5-dimethyl pyrazine 및 tri-methyl pyrazine 였으며, 청국장의 독특한 향미성분의 pyra-zine 류는 대두 청국장보다 키위와 무를 넣은 소립 검정콩 청국장에서 약 3배 정도 감소하였다. 주요 핵산관련 물질은 uracil과 UMP였으며, 다른것은 그 함량에 유사한 경향이었다.

• 한국균학회지
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• 제14권2호
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• pp.185-188
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• 1986

노랑 느타리버섯의 유전 연구 및 다른 느타리종과의 융합을 위해서 노랑 느타리의 담자포자에 자외선을 조사하여 영양요구성 균주를 선발하였다. 노랑 느타리 포자에 UV를 조사한 결과 UV조사시간이 $40{\sim}70$초이고 생존율 $0.4{\sim}1.4%$ 일때 많은 mutants를 얻을 수 있었고, 선발된 14 auxotrophic mutants의 유전 marker는 amino acid-requiring strain 3균주, nucleic acid base-requiring strain 1 균주, vitamin-requiring strain 3 균주, amino acid/nucleic acid base-requiring strain 2 균주, amino acid/vitamin-requiring strain 5 균주로 되었다.

• 한국식품영양학회지
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• 제7권2호
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• pp.114-118
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• 1994

In order to compare the contents of nucleic acid substances in various wines, the contents of nueleic acids were analyzed by HPLC. The contents of cytosine was found to decrease in the order of cherry wine > plum wine > dongdongju > chungha > pineapple wine > soju. The contents of guanine was found to decrease in the order of dongdonglu > chungha > pineapple wine > cherry wine > plum wine > soju. The contents of uridine was found to decrease in the order of dongdongju > chungha> cherry wine > pineapple wine > plum wine > soju. The contents of adenine was found to decrease in the order of dongdonglu > chungha > cherry wine > pineapple wine > plum wine > solu. The contents of guan oslne was found to decrease in the order of dongdongju > cherry wine > chungha > plum wine > pine-apple wine > soju. The contents of adenosine was found to decrease in the order of dongdongju > shun aha > cherry wine > plum me > pineapple wine > soju.