• 한국수소및신에너지학회논문집
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• 제33권6호
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• pp.808-818
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• 2022

In this research paper, we investigated the cryogenic line chill down characteristics of liquefied natural gas (LNG). A numerical analysis model was established and verified so that it can calculate the precise cooling characteristics of cryogenic fluid for the stable and safe utilization especially such as LNG and liquid hydrogen. The numerical modeling was programmed by C++ as an one-dimensional homogeneous model. The thermohydraulic cooling process was simulated using mass, momentum, energy conservation equations and appropriate heat transfer correlations. In this process, the relevant heat transfer correlations for nuclear boiling, transition boiling, film boiling, and single-phase heat transfer that can predict the experimental results were implemented. To verify the numerical modeling, several cryogenic line chill down experiments using LNG were conducted at the Korea Institute of Machinery & Materials (KIMM) LNG and Cryogenic Technology Center.

• 한국분무공학회지
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• 제12권1호
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• pp.18-29
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• 2007

Feedwater heaters of many nuclear power plants have recently experienced severe wall thinning damage, which will increase as operating time progresses. Several nuclear power plants in Korea have experienced wall thinning damage in the area around the impingement baffle - installed downstream of the high pressure turbine extraction steam line - inside number 5A and 5B feedwater heaters. At that point, the extracted steam from the high pressure turbine is two phase fluid at high temperature, high pressure, and high speed. Since it flows in reverse direction after impinging the impingement baffle, the shell wall of the number 5 high pressure feedwater heater may be affected by flow-accelerated corrosion. This paper describes the comparisons between the numerical analysis results using the FLUENT code and the down scale experimental data in an effort to determine root causes of the shell wall thinning of the high pressure feedwater heaters. The numerical analysis and experimental data were also confirmed by actual wall thickness measured by an ultrasonic test.

• 대한기계학회논문집B
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• 제31권1호
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• pp.1-7
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• 2007

Feedwater heaters of many nuclear power plants have recently experienced severe wall thinning damage, which will increase as operating time progresses. Several nuclear power plants in Korea have experienced wall thinning damage in the area around the impingement baffle - installed downstream of the high pressure turbine extraction steam line - inside number 5A and 5B feedwater heaters. At that point, the extracted steam from the high pressure turbine is two phase fluid at high temperature, high pressure, and high speed. Since it flows in reverse direction after impinging the impingement baffle, the shell wall of the number 5 high pressure feedwater heater may be affected by flow-accelerated corrosion. This paper describes the comparisons between the numerical analysis results using the FLUENT code and the down scale experimental data in an effort to determine root causes of the shell wall thinning of the high pressure feedwater heaters. The numerical analysis and experimental data were also confirmed by actual wall thickness measured by an ultrasonic test.

• Nuclear Engineering and Technology
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• 제54권5호
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• pp.1890-1901
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• 2022

We have assessed the applicability of the thermal-hydraulic system analysis code, SPACE, to a small modular reactor called SMART. For the assessment, the experimental data from a scale-down integral-test facility, SMART-ITL, were used. It was conformed that the SPACE code unrealistically calculates the safety injection flow rate through the CMT and SIT during a small-break loss-of-coolant experiment. This unrealistic behavior was due to the overprediction of interfacial heat transfer at the steam-water interface in a vertically stratified flow in the tanks. In this study, a special thermal-hydraulic component model has been developed to realistically calculate the interfacial heat transfer when a strong non-equilibrium two-phase flow is formed in the CMT or SIT. Additionally, we developed a special heat structure model, which analytically calculates the heat transfer from the hot steam to the cold tank wall. The combination of two models for the tank are called the special component model. We assessed it using the SMART-ITL passive safety injection system (PSIS) test data. The results showed that the special component model well predicts the transient behaviors of the CMT and SIT.

• Advances in nano research
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• 제10권3호
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• pp.263-270
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• 2021

The present manuscript focuses the effects of suction on the flow of the dusty fluid along permeable exponentially stretching cylinder. Derived PDEs for this work are changed into ODEs by adopting right transformations. Numerical procedure is carried out for the obtained resultant equations by Shooting Technique in accordance with Runge-Kutta (RK-6) technique. Obtained results for the parameters namely, particle interaction parameter, suction parameter and Reynold number parameters are probed thoroughly. Some salient points are: (a) Fluid velocity decreases and the dust phase velocity rises for the higher values of particle interaction parameter; (b) more suction produces retarding velocities for both the phases; (c) high Reynold number slows down the fluid velocity while the speed of dust phase and (d) skin friction coefficient goes high for all these parameters.

• 한국자기공명학회논문지
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• 제11권2호
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• pp.64-72
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• 2007

[ $^1H$ ] nuclear magnetic resonance (NMR) experiments have been performed at 30 - 300 K and 7 T to investigate dynamics of hydrogen bond network in the single crystal $(NH_4)_3H(SO_4)_2$. The two proton sites, ammonium proton and hydrogen-bond proton, are identified from the $^1H$ NMR MAS spectrum at 340 K. As temperature decreases, the $^1H$ NMR spectrum shifts to the higher frequency side with a larger linewidth. The spectrum at 65 K shows a distinctive change in line shape toward the ferroelectric transition at 63 K. The measured values of $T_1$ for ammonium and hydrogen-bond protons are similar in the whole range of temperature. $T_1$ of $^1H$ NMR shows a gradual decrease down to 120 K and starts to steeply increase below 100 K. Then $T_1$ shows abrupt decrease below 70 K with a sharp minimum at 63 K, where the ferroelectric transition occurs. This temperature dependence of spectrum and $T_1$ clearly prove that the large change in the dynamics of hydrogen bond network is associated with the ferroelectric phase transition at 63 K.

• The Korean Journal of Physiology and Pharmacology
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• 제19권5호
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• pp.421-426
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• 2015

The increased potential for vascular smooth muscle cell (VSMC) growth is a key abnormality in the development of atherosclerosis and post-angioplasty restenosis. Abnormally high activity of platelet-derived growth factor (PDGF) is believed to play a central role in the etiology of these pathophysiological situations. Here, we investigated the anti-proliferative effects and possible mechanism(s) of murrayafoline A, a carbazole alkaloid isolated from Glycosmis stenocarpa Guillamin (Rutaceae), on PDGF-BB-stimulated VSMCs. Murrayafoline A inhibited the PDGF-BB-stimulated proliferation of VSMCs in a concentration-dependent manner, as measured using a non-radioactive colorimetric WST-1 assay and direct cell counting. Furthermore, murrayafoline A suppressed the PDGF-BB-stimulated progression through $G_0/G_1$ to S phase of the cell cycle, as measured by [$^3H$]-thymidine incorporation assay and cell cycle progression analysis. This anti-proliferative action of murrayafoline A, arresting cell cycle progression at $G_0/G_1$ phase in PDGF-BB-stimulated VSMCs, was mediated via down-regulation of the expression of cyclin D1, cyclin E, cyclin-dependent kinase (CDK)2, CDK4, and proliferating cell nuclear antigen (PCNA), and the phosphorylation of retinoblastoma protein (pRb). These results indicate that murrayafoline A may be useful in preventing the progression of vascular complications such as restenosis after percutaneous transluminal coronary angioplasty and atherosclerosis.

• Molecules and Cells
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• 제37권12호
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• pp.857-864
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• 2014

Astrocyte elevated gene-1 (AEG-1) is a recently discovered oncogene that has been reported to be highly expressed in various types of malignant tumors, including renal cell carcinoma. However, the precise role of AEG-1 in renal cancer cell proliferation and apoptosis has not been clarified. In this study, we transfected the renal cancer cell line Caki-1 with a plasmid expressing AEG-1 short hairpin RNA (shRNA) and obtained cell colonies with stable knockdown of AEG-1. We found that AEG-1 down-regulation inhibited cell proliferation and colony formation and arrested cell cycle progression at the sub-G1 and G0/G1 phase. Western blot analysis indicated that the expression of proliferating cell nuclear antigen (PCNA), cyclin D1 and cyclin E were significantly reduced following AEG-1 down-regulation. In addition, AEG-1 knockdown led to the appearance of apoptotic bodies in renal cancer cells, and the ratio of apoptotic cells significantly increased. Expression of the antiapoptotic factor Bcl-2 was dramatically reduced, whereas the pro-apoptotic factors Bax, caspase-3 and poly (ADPribose) polymerase (PARP) were significantly activated. Finally, AEG-1 knockdown in Caki-1 cells remarkably suppressed cell proliferation and enhanced cell apoptosis in response to 5-fluorouracil (5-FU) treatment, suggesting that AEG-1 inhibition sensitizes Caki-1 cells to 5-FU. Taken together, our data suggest that AEG-1 plays an important role in renal cancer formation and development and may be a potential target for future gene therapy for renal cell carcinoma.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2527-2532
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• 2012

Objectives: The aim of the present study was to explore mechanisms underlying the effects of down-regulating ${\beta}$-catenin expression on esophageal carcinoma (EC) cells. Methods: Cell cycle distribution and apoptosis were determined using flow cytometry and annexin V apoptosis assay, respectively. Transmission electron microscopy (TEM) was used to examine changes in ultrastructure, while expression of cyclin D1 protein and mRNA was detected by western blot and real-time PCR. Proliferating cell nuclear antigen (PCNA) and extracellular signal-regulated kinase (ERK) 1-2 were evaluated by Western blot analysis. PCNA labeling index (LI) was determined by immunocytochemistry. Results: Compared with pGen-3-con transfected and Eca-109 cells, the percentage of G0/G1-phase pGen-3-CTNNB1 transfected cells was obviously increased (P<0.05), with no significant difference among the three groups with regard to apoptosis (P>0.05). pGen-3-CTNNB1 transfected cells exhibited obvious decrease in cyclin D1 mRNA and protein expression (P<0.05) and the ultrastructure of Eca-109 cells underwent a significant change after being transfected with pGen-3-CTNNB1, suggesting that down-regulating ${\beta}$-catenin expression can promote the differentiation and maturation. The expression of PCNA and the ERKI/2 phosphorylation state were also down-regulated in pGen-3-CTNNB1 transfected cells (P<0.05). At the same time, the PCNA labeling index was decreased accordingly (P<0.05). Conclusion: Inhibition of EC Eca-109 cellproliferation by down-regulating ${\beta}$-catenin expression could improve cell ultrastructure by mediating blockade in G0/G1 through inhibiting cyclin D1, PCNA and the MAPK pathway (p-ERK1/2).

• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 춘계학술대회논문집D
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• pp.821-827
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• 2001

As one of the advanced design features of the Korea next generation reactor, direct vessel injection (DVI) system is being considered instead of conventional cold leg injection (CLl) system. It is known that the DVI system greatly enhances the reliability of the emergency core cooling (ECC) system. However, there is still a dispute on its performance in terms of water delivery to the reactor core during the reflood period of a large-break loss-of-coolant accident (LOCA). Thus, experimental validation is under progress. In this paper, a new scaling method, using time and velocity reduced linear scaling law, is suggested for the design of a scaled-down experimental facility to investigate the direct ECC bypass phenomena in PWR downcomer.