• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.307-313
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• 1996

This study was conducted to investigate the effect of S-phase synthronized nuclear transfer on the development of nuclear transplant bovine embryos. A blastomere derived from the 16~32 cell stage bovine embryos was transferred into an enucleated metaphase II(MII) oocytes or activated S-phase eggs. From the MII-phase and S-phase nuclear transfer, 6.3%(4/63) and 13.8%(9/65) of nuclear transplant embryos developed to the blastocyst stage, respectively. In the S-phase nuclear transfer, maximal proportion of embryos developed to the blastocyst stage(16.6%) was obtained after the recipient cell was activated 8 h prior to receving a donor nucleus. MII-phase nuclear transplant embryos showed the PCC state of their nuclear at 1.5~2 h after fusion, whereas, S-phase nuclear transplant embryos did not undergo PCC. The result of this study suggests that if blastomeres of unknown cell-cycle-stage are used, S-phase nuclear transplantation through the activation of enucleated oocytes prior to fusion enhances development of nuclear transplant embryos. This result also suggests that the interval time from oocyte activation to cell fusion may affect the development of nuclear transplant embryos.

• The Korean Journal of Zoology
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• v.37 no.3
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• pp.318-323
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• 1994

The association of replication origins/termini with nuclear matrix during S phase was investigated by DNase digestion of halo structures in synchronized mouse LPI-1 cells. The binding of parental DNA to nuclear matrix was constant throughout S phase. When nuclear matrix was isolated from the cells pulse-labeled with 3H-thvmidine at various stases of S phase, total 3H-labels associated with nuclear matrix were specifically higher at So, Sa and Ss stages than other stases of S phase, suggesting that the newly synthesized DNAs at those stages are not excluded out of nuclear matrix. Similar patterns were obsenred from the pulse-chase experiments, in which cells were pulse-labeled at each stage of S phase and further incubated for 1 hr. These results suggest that the replication origins and termini are fixed at the nuclear matrix, and that the nuclear matrix binding fractions of DNA at 3C-pause may contain a large population of replication origins and termination sites.

• Nuclear Engineering and Technology
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• v.52 no.3
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• pp.610-613
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• 2020

Specimens of austenitic stainless steel were irradiated with 6 MeV Xe ions to two doses of 7 and 15 dpa at room temperature and 300 ℃ respectively. Then partial irradiated specimens were subsequently thermally annealed at 550 ℃. Irradiation-induced BCC-phase formation and magnetism were analyzed by grazing incidence X-ray diffraction (GIXRD) and vibrating sample magnetometer (VSM). It has been shown that irradiation damage level, irradiation temperature and annealing temperature have significant effect on BCC-phase formation. This BCC-phase changes the magnetic behavior of austenitic stainless steel. The stress relief and compositional changes in matrix are the driving forces for BCC-phase formation in austenitic stainless steel during ion irradiation.

• Nuclear Engineering and Technology
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• v.42 no.4
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• pp.365-376
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• 2010

This paper summarizes the results of a Writing Group on the Extension of CFD codes to two-phase flow safety problems, which was created by the Group for Analysis and Management of Accidents of the Nuclear Energy Agency' Committee on the Safety of Nuclear Installations (NEA-CSNI). Two-phase CFD used for safety investigations may predict small scale flow processes, which are not seen by system thermalhydraulic codes. However, the two-phase CFD models are not as mature as those in the single phase CFD and potential users need some guidance for proper application. In this paper, a classification of various modelling approaches is proposed. Then, a general multi-step methodology for using two-phase-CFD is explained, including a preliminary identification of flow processes, a model selection, and a verification and validation process. A list of 26 nuclear reactor safety issues that could benefit from investigations at the CFD scale is identified. Then, a few issues are analyzed in more detail, and a preliminary state-of-the-art is proposed and the remaining gaps in the existing approaches are identified. Finally, guidelines for users are proposed.

• Nuclear Engineering and Technology
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• v.55 no.9
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• pp.3241-3251
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• 2023

The potential for recriticality and sub-critical boron concentrations is analyzed during the relocation of the fuel rods in the assembly, which we call late phase of a severe accident, via coupling between MELCOR and whole-core Monte Carlo analyses by Serpent 2. The recriticality, initiated during the early phase, is found to maintain when the fuel assemblies containing intact fuel rods are submerged by the cooling water. It is also found that the effect of the negative reactivity insertion via remaining fission products in the fuel debris increases as the burnup increases. The sub-critical boron concentrations during the late phase are found to be 76~544 ppm lower than those during the early phase. Therefore, it can be concluded that the boron concentration that prevents recriticality not only during the early phase but also during the late phase is the sub-critical boron concentration during the early phase.

• Nuclear Engineering and Technology
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• v.56 no.1
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• pp.19-33
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• 2024

In nuclear thermal-hydraulic system codes, most correlations used for vertical pipes, under downward two-phase flow, have been developed considering small pipes or pool systems. This suggests that there could be uncertainties in applying the correlations to accident scenarios involving large vertical pipes owing to the difference in the characteristics of two-phase flows, or flow conditions, between large and small pipes. In this study, we modified the Multi-dimensional Analysis of Reactor Safety KINS Standard (MARS-KS) code using correlations, such as the drift-flux model and two-phase multiplier, developed in a plant-scale air-inflow experiment conducted for a pipe of diameter 600 mm under downward two-phase flow. The results were then analyzed and compared with those based on previous correlations developed for small pipes and pool conditions. The modified code indicated a good estimation performance in two plant-scale experiments with large pipes. For the siphon-breaking experiment, the maximum errors in water flow for modified and original codes were 2.2% and 30.3%, respectively. For the air-inflow accident experiment, the original code could not predict the trend of frictional pressure gradient in two-phase flow as / increased, while the modified MARS-KS code showed a good estimation performance of the gradient with maximum error of 3.5%.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.143-153
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• 1996

To improve the efficiency of nuclear transplantation in the rabbit, this study were evaluated the influence of celly cycle of donor nuclei on the in vitro developmental potential in the nuclear transplant embryos. The embryos of 16-cell stage were collected from the mated does at 48h post-hCG injection and they were synchronized to G1 phase of 32-cell stage. Synchronization of the cell cylce of blastomeres were induced, first, using an microtubules polymerization inhibitor, 0.5$\mu\textrm{g}$/ml colcemid for 10h to arrest blastomeres in metaphase, and secondly, using a DNA synthesis inhibitor, 0.1$\mu\textrm{g}$/ml aphidicolin for 1.5 to 2h to cleave to 32-cell stage and arrest them in G1 phase. The separated G1 phase blastomeres of 32-cell stage were injectied into enucleated recipient cytoplasms by micromanipulation. After culture until 20h post-hCG injection, the nuclear transplant oocytes were electrofused and activated by electrical stimulation. The nuclear transplant embryos were co-cultured for 120h. In vitro cultured embryos were monitored every 24h to assess for development rate. After in vitro cultue for 120h, the nuclear transplant embryos developed to blastocyst stage were stained with Hoechst 33342 dye for counting the number of blastomeres under a fluorescence microscopy. The cleavage rate of blastomeres from 16-cell stage stage rabbit embryos treated with colcemid for 10h or aphidicolin for 6h following colcemid for 10h were not significantly different. The electrofusion rate was similar by high in S and G1 phase donor nuclei as 80.6 and 79.1%, respectively. However, the nuclear transplant embryos using G1 phase donor nuclei were developed to blastocyst at high rate(60.3%) than those using S phase donor nuclei(26.0%). Moreover, the mean blastocyst stage were increased significantly(P<0.05) with the G1 phase donor nuclei(176.6 cells and 1.50 cycles), as compared with the S phase donor nuclei(136.6 cells and 1.42 cycles). These results show that the blastomeres of G1 phase were more successful as donor nuclei in the nuclear transplant procedure, compared with S phase.

• Nuclear Engineering and Technology
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• v.51 no.3
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• pp.761-768
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• 2019

Understanding the phenomena of formation of single drops is necessary to understand the hydrodynamics in solvent extraction equipment which are used for separation of nuclear materials. In this work, the phenomena of aqueous phase and organic phase drop formation at submerged nozzles are compared by conducting experiments with 30%TBP (v/v) in dodecane as the organic phase and nitric acid as the aqueous phase. Two different nozzles and three different nitric acid concentrations are used. For each nozzle and nitric acid concentration, velocity of the dispersed phase is varied. Drops of aqueous phase formed at downward oriented nozzles submerged in organic phase are observed to be smaller than the drops of organic phase formed at upward oriented nozzles submerged in aqueous phase. Correlations to estimate drop diameter are proposed.

• Nuclear Engineering and Technology
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• v.45 no.6
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• pp.821-826
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• 2013

In the present study, the effects of various heat treatments on the microstructure and mechanical properties of dual phase ODS steels were investigated to enhance the high strength at elevated temperature. Dual phase ODS steels have been designed by the control of ferrite and austenite formers, i.e., Cr, W and Ni, C in Fe-based alloys. The ODS steels were fabricated by mechanical alloying and a hot isostatic pressing process. Heat treatments, including hot rolling-tempering and normalizing-tempering with air- and furnace-cooling, were carefully carried out. It was revealed that the grain size and oxide distributions of the ODS steels can be changed by heat treatment, which significantly affected the strengths at elevated temperature. Therefore, the high temperature strength of dual phase ODS steel can be enhanced by a proper heat treatment process with a good combination of ferrite grains, nano-oxide particles, and grain boundary sliding.

• Nuclear Engineering and Technology
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• v.51 no.8
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• pp.2026-2033
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• 2019

Fluidelastic instability of a tube array is a key factor of the security of a nuclear power plant. An unsteady model of the fluidelastic instability of a tube array subjected to two-phase flow was developed to analyze the fluidelastic instability of tube bundles in two-phase flow. Based on this model, a computational program was written to calculate the eigenvalue and the critical velocity of the fluidelastic instability. The unsteady model and the program were verified by comparing with the experimental results reported previously. The influences of void fraction and the tube's material properties on the critical velocity were investigated. Numerical results showed that, with increasing the void fraction of the two-phase flow, the tube array becomes more stable. The results indicate that the critical velocities of the tube array made of stainless are much higher than those of the other two tube arrays within void fraction ranging from 20% to 80%.