• Journal of the Korean Society for Nondestructive Testing
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• v.29 no.2
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• pp.153-161
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• 2009

After several PWSCCs were found in Bugey(France), Ringhals(Sweden), Tihange(Belgium), Oconee, Arkansas, Crystal Fever, Davis-Basse, VC Summer(U.S.A.), Thuruga(Japan), USNRC and PNNL started the research on PWSCC, that is, PINC project. The aim of this project is to fabricate and obtain representative NDE mock-ups with flaws to simulate tight PWSCC cracks, to identify and quantitatively assess NDE methods for accurately detecting, sizing and characterizing tight cracks such as PWSCC, to document the range of locations and crack morphologies associated with PWSCC and observed responses and to incorporate findings from other ongoing PWSCC research programs, as appropriate. By participating in PINC project, Korean morphology technique about PWSCC and NDE technique have improved and become similar lever with other advanced country. Therefore, the evaluation technique of integrity for nickel alloy component has been improved by cooperation with university, research institute and industries.

• Nuclear Engineering and Technology
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• v.55 no.5
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• pp.1644-1650
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• 2023

In spent fuel reprocessing, UO₂(DBP)₂ (U-DBP) can be deposited in stainless steel equipment. U-DBP must be removed by dissolution and the process must not cause corrosion to stainless steel. This study was conducted to find the best scheme for dissolution. U-DBP was manufactured by the titrimetric sedimentation method. The effects of different factors on the dissolution of U-DBP were investigated. For example, solid-liquid ratio, hydrazine carbonate solutions with different mass components, mixed solutions containing different concentrations of H₂O₂, and different carbonates. The results indicated that U-DBP does not have a regular crystal morphology. With the increase of the solid-liquid ratio and the mass fraction of hydrazine carbonate, the concentration of U(VI) at the dissolution equilibrium increases gradually. The addition of H₂O₂ has a great promotion effect on the dissolution. However, when the concentration of H₂O₂ is greater than 0.5 M, the dissolution solution may have an erosive effect on the stainless steel. (NH₄)₂CO₃ can increase the dissolution capacity of dissolved U-DBP, but it may also accelerate the corrosion of stainless steel.

• International Journal of Industrial Entomology and Biomaterials
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• v.43 no.1
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• pp.6-15
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• 2021

We investigated the taxonomic status of the Korean populations of Eurema hecabe (Linnaeus, 1758) based on morphology and nuclear triose phosphate isomerase (Tpi). Up to now, the Korean population of E. hecabe was classified into E. mandarina (de l'Orza, 1869) based on morphological characteristics. A previous study raised the possibility of the Jeju-do population to be E. hecabe based on the color of forewing's fringe. However, morphological examination showed that the Korean population found in southern areas (Gyeonsang and Jeolla provinces), including Jeju-do is E. mandarina. In particular, the external morphology of autumn seasonal type of the Korean population was almost same as the Japanese mainland population, which is known as E. mandarina. Furthermore, sequence analysis of Tpi gene from nine individuals of E. mandarina collected in South Korean localities including Jeju-do clearly showed that all Korean specimens truly belong to E. mandarina.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.75-81
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• 2001

We have constructed a novel recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV) producing the green fluorescent polyhedra. For the production of the fluorescent polyhedra, partial polyhedrin gene containing KRKK as nuclear localization site from the BmNPV polyhedrin gene and the green fluorescent protein (gfp) gene were introduced under the control of p10 promoter of BmNPV. The recombinant BmNPV was stably produced fluorescent polyhedra in the infected Bm5 cells and the morphology of the fluorescent polyhedra was similar to that of wild-type BmNPV. The fluorescent polyhedra had 32 kDa native polyhedrin and 41 kDa fusion protein. From these data, we have further developed a novel BmNPV p10-based transfer vector producing recombinant polyhedra with foreign gene Product. The novel BmNPV P10-based transfer vector is composed of partial polyhedrin gene, factor Xa, and multiple cloning sites.

• Korean Journal of Materials Research
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• v.15 no.3
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• pp.172-176
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• 2005

Formation of aluminum hydroxide by a hydrolytic reaction of nano aluminum powder synthesized by a pulsed wire evaporation (PWE) method has been studied. The type and morphology of the hydroxides were investigated with various initial temperatures and pHs. The nano fibrous boehmite (AlOOH) was formed predominantly over $40^{\circ}C$ of the hydrolytic temperature in acid solution, while the bayerite $(Al(OH)_3)$ was formed predominantly below $30^{\circ}C$ in alkali solution with a faceted crystalline structure. As a result the boehmite showed a much larger specific surface area (SSA) than that of bayerite. The highest SSA of the boehmite was about $409\;m^2/g$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.3
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• pp.171-175
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• 2000

The ultrastructures of germinal cells of male marsh clam, Corbicujar lena were studied. The mature sperm was primitive type, consisting of head, middle piece and tail. The mature sperm was whip-shaped and its head was divided into two parts; the acrosomal part shaped long hollow cone about $3{\mu}m$ in length and the sperm nuclear part shaped a long stick about $9\;{\mu}m$ in length. The posterior part of the sperm nuclear projected to centriole, The middle piece of the sperm-nuclear had four mitochondria and two centrioles. The sperm tail part had the 9+2 microtubular arrangement known as a typical pattern, During spermiogenesis, chromatin within sperm nuclear became fiberic materials by condensation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.5
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• pp.480-485
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• 2003

Morphology of the spermatozoa from the testes of the catfish (Pseudobagrus brevicorpus) was studied by transmission and scanning electron microscopy. The spermatozoa of P. brevicorpus are approximately $82.25\pm0.06\;{\mu}m$ in length and relatively simple cells composed of a spherical head, a short midpiece and a tail as in most teleost fish, The nucleus measuring about $2.00\pm0.02\;{\mu}m$ in length is depressed with a deep nuclear fossa of about $1.05\pm0.03\;{\mu}m$ in length three fifths of the nuclear length. The nuclear fossa contains the proximal and distal centrioles. The two centrioles are oriented approximately $150^{\circ}$ to each other. The mitochondria are arranged in two layers and their number is 12 or more. They are separated from the axoneme by the cytoplasmic canal. The axoneme is the 9+2 microtubular pattern and has inner but no outer dynein arms as in other bagrids. The axonemal fins were the closed to axonemal doublet 3 and 8. The axonemal fins and lost outer dynein arm are shared in Bagridae and the deep nuclear fossa is shared in Siluriformes. The axonemal fins observed in Bagridae and Amblycipitidae of Siluriformes might be the apomorphic character in Ostariophysi.

• Molecules and Cells
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• v.26 no.3
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• pp.270-277
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• 2008

This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.

• Journal of Embryo Transfer
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• v.32 no.3
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• pp.81-86
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• 2017

The Somatic cell nuclear transfer (SCNT) method can be applied to various fields such as species conservation, regenerative medicine, farming industries and drug production. However, the efficiency using SCNT is very low for many reasons. One of the troubles of SCNT is that it is highly dependent on the researcher's competence. For that reason, four somatic cell nuclear injection methods were compared to evaluate the effect of hole-sealing process and existence of cytochalasin B (CB) on efficiency of murine SCNT protocol. As a results, the microinjection with the hole-sealing process, the oocyte plasma membrane is inhaled with injection pipette, in HCZB with CB was presented to be the most efficient for the reconstructed in SCNT process. In addition, we demonstrated that the oocytes manipulated in Hepes-CZB medium (HCZB) with CB does not affect the developmental rate and the morphology of the blastocyst during the pre-implantation stage. For this reason, we suggest the microinjection involving hole-sealing in HCZB with CB could improve SCNT process efficiency.

• Nuclear Engineering and Technology
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• v.56 no.6
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• pp.2324-2331
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• 2024

The current study investigated Saudi Arabian oil fly ash impacts on Egyptian clay bricks' structural and radiation shielding properties. To produce the required bricks, crushed clay minerals from the Hafafit area were mixed with 0, 10, 20, 30, and 40 % wt.% Saudi Arabian oil fly ash and pressed at a pressure rate of 68.55 MPa. Identification of the minerals in the chosen clay was achieved via X-ray diffraction. Additionally, the material's morphology and chemical composition were determined through scanning electron microscope and energy-dispersive X-ray. The fabricated bricks' density was reduced by 36.3 % through increasing the concentration of fly ash from 0 to 40 wt%. Then, the fly ash addition's influence on the fabricated clay bricks' γ-ray shielding properties was investigated by Monte Carlo simulation, which found a reduction in the fabricated bricks' linear attenuation coefficient (LAC) by 41.2, 36.0, 33.8, and 33.8 % at the 0.059, 0.103, 0.662, and 1.252 MeV γ-ray energies, respectively. The LAC reduction caused an increase in the fabricated bricks' half-value thickness, transmission factor, and the equivalent thickness of the lead. Moreover, the thicker fabricated sample thicknesses were found to have high γ-ray shielding capacity and can thus be used in radiation shielding applications.