• Molecules and Cells
• /
• 제27권5호
• /
• pp.539-546
• /
• 2009

In Saccharomyces cerevisiae, ribosomal protein L7, one of the ~46 ribosomal proteins of the 60S subunit, is encoded by paralogous RPL7A and RPL7B genes. The amino acid sequence identity between RPl7a and RPl7b is 97 percent; they differ by only 5 amino acid residues. Interestingly, despite the high sequence homology, Rpl7b is detected in both the cytoplasm and the nucleolus, whereas Rpl7a is detected exclusively in the cytoplasm. A site-directed mutagenesis experiment revealed that the change in the amino acid sequence of Rpl7b does not influence its subcellular localization. In addition, introns of RPL7A and RPL7B did not affect the subcellular localization of Rpl7a and Rpl7b. Remarkably, Rpl7b was detected exclusively in the cytoplasm in rpl7a knockout mutant, and overexpression of Rpl7a resulted in its accumulation in the nucleolus, indicating that the subcellular localization of Rpl7a and Rpl7b is influenced by the intracellular level of Rpl7a. Rpl7b showed a wide range of localization patterns, from exclusively cytoplasmic to exclusively nucleolar, in knockout mutants for some rRNA-processing factors, nuclear pore proteins, and large ribosomal subunit assembly factors. Rpl7a, however, was detected exclusively in the cytoplasm in these mutants. Taken together, these results suggest that although Rpl7a and Rpl7b are paralogous and functionally replaceable with each other, their precise physiological roles may not be identical.

• 한국발생생물학회지:발생과생식
• /
• 제23권3호
• /
• pp.263-275
• /
• 2019

Based on our preliminary results, we examined the possible role of low-dose and chronic-exposing of the chemicals those are known as endocrine disrupting chemical (EDC), on the proliferation of uterine endometrium and the localization of steroid receptors. Immunohistochemical or immunofluorochemical methodology were employed to evaluate the localization of antigen identified by monoclonal antibody Ki 67 protein (MKI67), estrogen receptor 1 (ESR1), estrogen receptor 2 (ESR2), and progesterone receptor (PGR). In $133{\mu}g/L$ and $1,330{\mu}g/L$ di(2-ethylhexyl) phthalate (DEHP) and $50{\mu}g/L$ nonylphenol (NP) groups, the ratio of MKI67 positive stromal cells was significantly increased but not in $500{\mu}g/L$ NP group. The ratios of MKI67 positive glandular and luminal epithelial cells were also changed by the chronic administration of NP and DEHP in tissue with dose specific manner. ESR1 signals were localized in nucleus in glandular and luminal epithelia of control group but its localization was mainly in cytoplasm in DEHP and NP administered groups. On the other hand, it was decreased at nucleus of stromal cells in $1,330{\mu}g/L$ DEHP group. The colocalization patterns of these nuclear receptors were also modified by the administration of these chemicals. Such a tissue specific and dose specific localization of ESR2 and PGR were detected as ESR1 in all the uterine endometrial tissues. These results show that the chronic lows-dose exposing of NP or DEHP modify the localization and colocalization of ESRs and PGR, and of the proliferation patterns of the endometrial tissues.

• 제어로봇시스템학회논문지
• /
• 제22권5호
• /
• pp.367-373
• /
• 2016

In this paper, range measurement performance of LRF (Laser Range Finder) module and image contrast of color CCD camera are evaluated under the aerosol (high temperature steam) environments, which are simulated severe accident conditions of the LWR (Light-Water-Reactor) nuclear power plant. Data of LRF and color CCD camera are key informations, which are needed in the implementation of SLAM (Simultaneous Localization and Mapping) function for emergency response robot system to cope with urgently accidents of the nuclear power plant.

• 기술사
• /
• 제44권6호
• /
• pp.40-44
• /
• 2011

Nuclear energy in Korea began in 1958, when the Korea's atomic energy act was formulated and the relevant organizations were founded. Since then, notwithstanding the two catastrophe like TMI and Chernobyl accident, Korea made a wise decision to expand the peaceful uses of the nuclear energy as well as to localize the essential nuclear design technology of fuel and nuclear steam supply system. This decision resulted in the success of export of nuclear power plants as well as research reactor in 2010s. The Korea's nuclear policy, which well utilized 'international crisis in nuclear business' as 'opportunity of Korea to get. nuclear technology', is believed nice policy as a role model of nuclear new-comer countries. Based upon the success story of localization of nuclear technology, Korea had an eye for a niche market, which was a basis of development of SMART, Korea-made integral PWR. The operation of a SMART plant can sufficiently provide not only electricity but also fresh water for 100,000 residents. Last two years, Korea's nuclear industry team led by the Korea Atomic Energy Research Institute completed the standard design of SMART and applied to the Korea's regulatory body for standard design approval. Now the Korea's licensing authority is reviewing the design with the relevant documents, and the design team is doing its best to realize its hope to get the approval by the end of this year. From next year, the SMART business including construction and export will be explored by the KEPCO consortium.

• 생명과학회지
• /
• 제25권8호
• /
• pp.947-952
• /
• 2015

Par-4는 종양 억제 유전자로 암세포 선택적으로 세포사멸을 증진하는 기능을 가진다. Par-4 유전자는 nuclear localization sequences (NLS), leucine zipper (LZ), nuclear export sequence (NES), selective for apoptosis in cancer cells (SAC)의 네 가지 도메인을 가지고 있다. 이 중에서도 SAC 도메인이 Par-4에 의한 세포사멸에 중요한 역할을 하며, 이러한 Par-4의 활성화는 세포 내 경로와 세포 외 경로로 나누어진다. 세포질 내의 Par-4는 핵 내로 이동하여 NF-κB 매개의 세포 성장 경로를 억제하고 세포 밖으로 분비된 Par-4는 세포 표면에 존재하는 수용체인 GRP78과 결합하여 세포 사멸을 유도한다. 따라서 Par-4의 발현을 증가시키는 물질에 의한 세포 사멸뿐만 아니라 암세포에서 발현이 낮은 Par-4의 과발현을 통하여 세포사멸 민감화가 증진된다. 따라서 Par-4는 암 치료의 강력한 표적으로의 가능성을 가지고 있다.

• 한국시스템다이내믹스연구
• /
• 제7권2호
• /
• pp.57-80
• /
• 2006

Nuclear technology made a great contribution to the national economy and society by localization of nuclear power plant design, and by stabilization of electricity price, etc. It is very important to conduct the retrospective analysis for the nuclear technology contribution to the national economy and society, but it is more important to conduct prospective analysis for the nuclear technology contribution. The term "technology value" is often used in the prospective analysis to value the result of technology development. There are various definitions of technology value, but generally it means the increment of future revenue or the reduction of future cost by technology development. These technology valuation methods are widely used in various fields (information technology or energy technology, etc). The main objective of this research is to develop valuation methodology that represents unique characteristics of nuclear power technology. The valuation methodology that incorporates market share changes of generation technologies was developed. The technology valuation model which consists of five modules (electricity demand forecast module, technology development module, market share module, electricity generation module, total cost module) to incorporate market share changes of generation technologies was developed. The nuclear power technology value assessed by this technology valuation model was 3 times more than the value assessed by the conventional method. So it was confirmed that it is very important to incorporates market share changes of generation technologies. The valuation results of nuclear power technology in this study can be used as policy data for ensuring the benefits of nuclear power R&D (Research and Development) investment.

• 대한핵의학회:학술대회논문집
• /
• 대한핵의학회 1992년도 제31차 춘계학술대회
• /
• pp.203.1-203.1
• /
• 1992

• Annals of Pediatric Endocrinology and Metabolism
• /
• 제23권4호
• /
• pp.204-209
• /
• 2018

Purpose: Growth hormone transduction defect (GHTD) is characterized by severe short stature, impaired STAT3 (signal transducer and activator of transcription-3) phosphorylation and overexpression of the cytokine inducible SH2 containing protein (CIS) and p21/CIP1/WAF1. To investigate the role of p21/CIP1/WAF1 in the negative regulation of the growth hormone (GH)/GH receptor and Epidermal Growth Factor (EGF)/EGF Receptor pathways in GHTD. Methods: Fibroblast cultures were developed from gingival biopsies of 1 GHTD patient and 1 control. The protein expression and the cellular localization of p21/CIP1/WAF1 was studied by Western immunoblotting and immunofluorescence, respectively: at the basal state and after induction with $200-{\mu}g/L$ human GH (hGH) (GH200), either with or without siRNA CIS (siCIS); at the basal state and after inductions with $200-{\mu}g/L$ hGH (GH200), $1,000-{\mu}g/L$ hGH (GH1000) or 50-ng/mL EGF. Results: After GH200/siCIS, the protein expression and nuclear localization of p21 were reduced in the patient. After successful induction of GH signaling (control, GH200; patient, GH1000), the protein expression and nuclear localization of p21 were reduced. After induction with EGF, p21 translocated to the cytoplasm in the control, whereas in the GHTD patient it remained located in the nucleus. Conclusion: In the GHTD fibroblasts, when CIS is reduced, either after siCIS or after a higher dose of hGH (GH1000), p21's antiproliferative effect (nuclear localization) is also reduced and GH signaling is activated. There also appears to be a positive relationship between the 2 inhibitors of GH signaling, CIS and p21. Finally, in GHTD, p21 seems to participate in the regulation of both the GH and EGF/EGFR pathways, depending upon its cellular location.

• 식물조직배양학회지
• /
• 제27권5호
• /
• pp.407-409
• /
• 2000

Outer envelope membrane proteins of chloroplasts encoded by the nuclear genome are transported without the N-terminal transit peptide. Here, we investigated the targeting mechanism of AtOEP7, an Arabidopsis homolog of small outer envelope membrane proteins in vivo. AtOEP7 was expressed transiently in protoplasts or stably in transgenic plants as fusion proteins with GFP. In both cases AtOEP7:GFP was targeted to the outer envelope membrane when assayed under a fluorescent microscope or by Western blot analysis. Except the transmembrane domain, deletions of the N- or C-terminal regions of AtOEP7 did not affect targeting although a region closed to the C-terminal side of the transmembrane domain affected the targeting efficiency. Targeting experiments with various hybrid transmembrane mutants revealed that the amino acid sequence of the transmembrane domain determines the targeting specificity The targeting mechanism was further studied using a fusion protein, AtOEP7：NLS：GFP, that had a nuclear localization signal. AtOEP7：NLS：GFP was efficiently targeted to the chloroplast envelope despite the presence of the nuclear localization signal. Taken together, these results suggest that the transmembrane domain of AtOEP7 functions as the sole determinant of targeting specificity and that AtOEP7 may be associated with a cytosolic component during translocation to the chloroplast envelope membrane.

• 한국음향학회지
• /
• 제16권6호
• /
• pp.76-84
• /
• 1997

원전내 금속파편들을 조기에 탐지하기 위한 금속파편 감시계통(LPMS : Loose Parts Monitoring System)은 원전의 안전성 및 신뢰도 확보를 위하여 중요한 부분으로서, 대부분의 국내 원전들에서 설치 운영중이거나 운영예정이다. 하지만 이들 LPMS들은 외국에서 개발된 것들로서 고가이며 기술이전이 이루어지지 않아 기술종속의 우려와 함께 효과적인 금속파편 진단에 많은 어려움을 지닌다. 따라서 본 논문의 주된 목적은 고해상도를 가지며 분석방법이 간단한 효율적인 금속파편 위치평가를 위한 알고리즘을 제안, 실현하므로써, 빈전문적인 운전자도 컴퓨터를 사용한 간단한 조작을 통하여 정확하고 신속한 금속파편 진단을 수행할 수 있도록 하는 것이다. 본 논문에서 제안한 수정된 원교차법을 이용하여 작성된 금속파편 위치평가 프로그램을 이용하여 실제 원전상황을 고려한 모의실험을 실시한 결과, 제안된 평가기법이 약 3.4% 정도 오차를 가지는 우수한 위치평가를 수행함을 알 수 있었다.