• 에너지공학
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• 제15권2호
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• pp.127-137
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• 2006

화학연료 사용으로 야기된 환경문제, 경제문제를 해결하기 위한 방안으로 수소경제가 추진되고 있다. 원자력을 이용한 대량수소생산은 수소경제를 뒷받침하기 위한 현실적인 방안이다. 본 논문에서는 원자력수소 생산에 사용할 초고온가스로의 특징과 개발현황, 초고온가스로로부터 발생하는 고온의 열을 이용한 수소생산방법 중 유력시 되는 기술로서 요오드-황 열화학법, 황산하이브리드법, 고온전기분해법의 기술개발 현황과 방향을 소개한다.

• 한국미생물·생명공학회지
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• 제22권1호
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• pp.23-30
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• 1994

Intergeneric hybrids formed between Aspergillus oryzae var oryzae and Penicillium verruculosum F-3 were obtained by nuclear transfer technique. Several auxotrophic mutants isolated fromconidio-spores of the two strains mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were 3$\times $10$^{-5}$ $~$1$\times $10$^{-5}$. From observation of genetic stability, conidial size, DNA content, nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrid posses the 1.3$~$2.2 fold higher amylase activities than those of parental strains. It was also revealed that some hybids had different isozyme patterns compared to those of parental strains by amylase activity assays.

• 한국양식학회지
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• 제10권1호
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• pp.39-47
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• 1997

무지개송어, Oncorhynchus mykiss 와 산천어, O. masou 간 잡종 및 잡종 3배체가 유도되었다. 잡종 3배체의 3배체 유도율은 92%로 유도된 잡종 3배체의 초기 생존율은 잡종에 비해 높게 나타났다. 잡종의 염색체수는 2n=63 이었으며 잡종 3배체의 염색체수는 3n=93으로 나타났다. 잡종은 적혈구 세포 및 핵 크기에서 잡종유도시 사용된 무지개송어, 산천어의 적혈구 세포 및 핵 크기의 중간치를 나타냈으며 잡종 3배체는 잡종에 비해 배수화에 기인되어 큰 적혈구 세포 및 핵 크기를 나타내었다. 이러한 배수화는 인형성부위 수에서도 나타나 잡종은 1개 혹은 2개의 인형성부위를 보인 반면 잡종 3배체는 1개, 2개 혹은 3개의 인형성부위를 나타내었다.

• 자연과학논문집
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• 제15권1호
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• pp.79-88
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• 2005

효모에는 여러 가지 종류의 thiol peroxid se 동위 효소들인 cytoplasmic TPx I, cTPx II, cTPx III, mitochodrial TPx (mTPx), 및 nuclear TPx (nTPx)가 존재하고 있다. 특히 cTPx II는 다른 효모TPx와 비교해 볼 때 매우 낮은 peroxidase 활성을 보이나, cTPx II를 제거한 cTPx II mutant균주는 심하게 성장이 저해되는 특징을 보인다. 본 연구에서는 효모에서의 cTPx II의 생리학적 기능을 밝히는 연구의 첫 과정으로 cTPx II와 상호 작용하는 단백질을 탐색하였다. Sacchromyces cerevisiae genomic DNA library에서 yeast two-hybrid system을 이용하여 cTPx II와 상호 결합하는 단백질을 탐색하여 그 단백질들의 기능을 연구하여, 궁극적으로 cTPx II의 생리기능을 밝히는데 이 연구의 목적을 두었다.

• 상하수도학회지
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• 제20권6호
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• pp.893-904
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• 2006

To improve sensitivity of biosensor as yeast two-hybrid detection system for estrogenic activity of suspected chemicals, we tested effects of several combinations of the bait and fish components in the two-hybrid system on Saccharomyces cerevisiae inducted a chromosome-integrated lacZ reporter gene that was under the control of CYC1 promoter and the upstream Gal4p-binding element $UAS_{GAL}$. The bait components that were fused with the Gal4p DNA binding domain are full-length human estrogen receptor ${\alpha}$ and its ligand-binding domain. The fish components that were fused with the Gal4p transcriptional activation domain were nuclear receptor-binding domains of co-activators SRC1 and TIF2. We found that the combination of the full-length human estrogen receptor ${\alpha}$ with the nuclear receptor-binding domain of co-activator SRC1 was most effective for the estrogen-dependent induction of reporter activity among the two-hybrid systems so far reported. The relative strength of transcriptional activation by representative natural and xenobiotic chemicals was well correlated with their estrogenic potency that had been reported with other assay systems.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2005년도 춘계학술발표회
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• pp.771-772
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• 2005

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1999년도 추계학술발표회요약집
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• pp.54.2-54
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• 1999

• 한국작물학회지
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• 제43권2호
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• pp.59-63
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• 1998

Three different male-sterile cytoplasmic lines and their common maintainer 'Zhenshan 97B' and two elite restorer lines were used to study cytoplasmic effects on agronomic trait manifestation per se under different nitrogen supply levels. The result showed that cytoplasmic effects could be modified by nitrogen environments. The cytoplasmic effect on grain yield under 150 kg N/ha varied depending on crosses, while it was significantly negative in most crosses under both 60 and 330 kg N/ha. The correlation and path-coefficient analyses suggested that it was expected to improve cytoplasmic effects through reducing maximum tillers and increasing the percentage of productive tillers, leading to increased productive tillers and higher yield in hybrid rice by the aid of cultural practice and genetic transformation. This study also revealed that the same cytoplasm in different combinations had differential effect under the same nitrogen environment, indicating that cytoplasmic effect was produced by interaction of nuclear genes with cytoplasm rather than cytoplasm per se. These results indicated the usefulness of evaluating diverse cytoplasmic sources in various nuclear genotypes bred for hybrid rice breeding program. The finding also suggested that negative cytoplasmic effect could be effectively overcome by elite restorer lines through the interaction of nuclear genes with female cytoplasm.

• Journal of Radiation Protection and Research
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• 제29권4호
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• pp.269-273
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• 2004

고계수율 환경에서의 G-M 계수기의 가용 범위를 확장하기 위하여 두 가지 불감시간(연장가능 및 연장불능)을 채택한 복합 모형이 개발되었으며, 이 복합모형 참 계수율과 실측 계수율간의 상관관계를 보다 정확히 설명한다. 이 논문에서는 몬테칼로 모사법에 근거한 G-M 계수기 불감효과 분석 프로그램 GMSIM을 개발하여 연장가능 불감시간 모형 및 연장불능 불감시간 모형에 적용하여 그 정확도를 확인하였다. GMSIM을 이용하여 복합 불감시간 모형을 따르는 G-M 계수기의 계수 통계 특성을 분석한 결과, 두 가지 이상적 모형의 중간적 특성을 보였다. 향후 GMSIM은 세 가지 모형의 불감시간 특성을 분석하는데 사용될 수 있다.

• Parasites, Hosts and Diseases
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• 제59권2호
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• pp.179-182
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• 2021

Human infection with Taenia asiatica or a hybrid between Taenia saginata and T. asiatica has not been reported in Cambodia. We detected for the first time a hybrid form between T. saginata and T. asiatica in Preah Vihear Province, Cambodia. An adult tapeworm specimen, i.e., 75 cm long strobila without scolex, was expelled from a 27-year-old man after praziquantel medication and purging. It was morphologically indistinguishable between T. saginata and T. asiatica. Several proglottids were molecularly analyzed to confirm the tapeworm species. The mitochondrial gene encoding cytochrome c oxidase subunit 1 (cox1) and nuclear genes encoding elongation factor-1α (ef1) and ezrin-radixin-moesin (ERM)-like protein (elp) were sequenced, and a single-allele analysis was performed to confirm the haploid genotype. The results revealed that our sample showed a discrepancy between the mitochondrial and 2 nuclear genes. It possessed homozygous sequences typical of T. saginata at cox1 and ef1 loci. However, it was heterozygous at the elp locus, with 1 allele in T. asiatica (elpA) and 1 in T. saginata (elpC), which indicates that it is a hybrid between T. saginata and T. asiatica. The present results confirmed the presence of a hybrid between T. saginata and T. asiatica in Cambodia and strongly suggest the existence of also 'pure' T. asiatica in Cambodia.