• 디지털콘텐츠
• /
• 3호통권58호
• /
• pp.68-73
• /
• 1998

윈도우 NT가 IT환경을 주도해 나갈 차세대 운영체제로 자리를 잡아가고 있다. 데스크탑 및 웍그룹 수준의 운영체제로 알려진 NT가 이제 릴리즈 5의 출시를 앞두고 본격적인 엔터프라이즈 시장 공략을 해나가고 있기 때문이다. 그동안 NT가 유닉스 시장을 대체할 수 있을지의 여부를 점쳐오던 주변 업체들도 이제 NT쪽 줄서기로 전열을 정비하고 있다. 따라서 올해 윈도우 NT가 운영체제 시장의 천하통일을 할 수 있을지에 관심이 모아지고 있다.

• 한국산학기술학회논문지
• /
• 제18권1호
• /
• pp.295-301
• /
• 2017

보툴리눔 신경독소는 콜린성 신경말단(부)을 선택적으로 공격하여 신경마비를 일으키는 신경독소로서, 그람양성을 띠고 내성포자를 형성하는 절대혐기성 세균인 보툴리눔 균(Clostridium botulinum)이 만들어낸다. 이 중 보툴리눔 A형 독소(BoNT/A)는 음식물과 물을 오염시킬 수 있으며 생물 무기나 생물 협박물질로 사용될 수도 있다. 이 때문에 독성을 탐지할 수 있는 예민한 분석방법과 중독을 치료할 수 있는 효능 있는 항독소를 개발해낼 필요성이 제기되어 왔다. 본 연구에서는 BoNT/A를 중화할 수 있는 단일클론 항체(mAb)를 생산하기 위하여 BoNT/A로 면역된 토끼의 항혈청에서 유래한 scFv 라이브러리를 인간 IgG와 융합시켰다. 그렇게 재조합된 scFvIgG 항체 단백질을 안정된 세포주에서 발현시켰고 항체 친화 크로마토그래피를 사용하여 scFvIgG mAb 단백질을 정제하였다. ELISA로 정제된 scFvIgG mAb 단백질의 효율성을 확인하였고, in vivo 실험으로 BoNT/A에 대한 중화능을 시험하였다. 독성 중화능 실험은 마우스를 사용하여 수행하였는데, 그 결과 scFvIgG 항체(10 ug)는 BoNT/A(100,000 $LD_{50}$)의 독성이 주입된 마우스를 완전히 방어하지는 못하지만 마우스의 생존 기간을 현격하게 연장시키는 것이 확인되었다. 이러한 결과들은 이 scFvIgG mAb가 BoNT/A를 중화하는 효능을 가지고 있다는 점을 제시한다.

• 제어로봇시스템학회논문지
• /
• 제17권1호
• /
• pp.38-46
• /
• 2011

The OPRoS (Open Platform for Robotic Service) framework provides uniform operating environment for service robots. As an OPRoS-based service robot has to support real-time as well as non-real-time applications, application of Windows NT kernel based operating system can be restrictive. On the other hand, various benefits such as rich library and device support and abundant developer pool can be enjoyed when service robots are built on Windows NT. The paper presents a user-mode component scheduler of OPRoS, which can provide near real-time scheduling service on Windows NT based on the restricted real-time features of Windows NT kernel. The component scheduler thread with the highest real-time priority in Windows NT system acquires CPU control. And then the component scheduler suspends and resumes each periodic component executors based on its priority and precedence dependency so that the component executors are scheduled in the preemptive manner. We show experiment analysis on the performance limitations of the proposed scheduling technique. The analysis and experimental results show that the proposed scheduler guarantees highly reliable timing down to the resolution of 10ms.

• 한국작물학회지
• /
• 제47권5호
• /
• pp.384-389
• /
• 2002

No-tillage (NT) practice for corn production has advantages of reduction of soil erosion and energy conservation. Research on effects of NT for sweet corn or super sweet corn is very limited. Hybrids of sugary (su) and shrunken-2 (sh2) were tested under NT and conventional tillage (CT) practices to investigate plant characters, ear characters, fresh yield, and grain yield. Sugary hybrids were Golden Cross Bantam 70 (GCB70), Sprint, Geumdanok, and Danok3. Shrunken-2 hybrids were BSS9472, Cambella90, GSS9299, Jubilee, KS-Y-65, and Chodangok1. Emergence rates under NT were lower than those under CT for su, while there was no difference between tillage systems for sh2. There were no differences between CT and NT for days to tasseling and silking, plant height, and ear height for both su and sh2. Ear characters such as ear length, number of kernel rows, number of kernels per row, and t100-kernel weight under NT were not significantly different from those under CT. There were no differences between two tillage practice for fresh and grain yield, rather they showed trend of increases under NT practices. Results from this trial indicate that NT practice for both su and sh2 cultivation may be possible to recommend to farmers.

• 한국자원식물학회지
• /
• 제23권6호
• /
• pp.535-540
• /
• 2010

Phenylalanine ammonia-lyase (PAL), a key enzyme of the phenylpropanoid biosynthesis pathway, is activated by a number of developmental and environmental cues. The coding region of the NtPAL4 gene was 2,154 bp in length, and its deduced protein was composed of 717 amino acids. Sequence analysis of NtPAL4 cDNA from tobacco (Nicotiana tabacum L.) revealed high structural similarity to PAL genes of other plant species. The NtPAL4 gene exists as a single copy in the tobacco plant, and its transcripts were strongly expressed in flowers and leaves. NtPAL4 expression was significantly induced in response to NaCl, mannitol, and cold treatments, but it was not induced by abscisic acid (ABA). NtPAL4 expression decreased gradually after treatment with ABA and $H_2O_2$; however, NtPAL4 transcripts accumulated after treatment with methyl viologen (MV). Our results suggest that the NtPAL4 gene may function in response to abiotic stresses.

• 한국응용곤충학회지
• /
• 제35권1호
• /
• pp.85-90
• /
• 1996

국내에서 분리된 Bacillus thuringiensis NT0423은 나비목과 파리목 곤충에 독성을 보이는 130 kDa의 전형적인 다이아몬드형 내독소 단백질을 생성한다. 이 균주의 파리목에 대한 독성을 강화하기 위하여, B. thuringeinsis NT0423에 모기 유충에 강한 독성을 보이는 B. thuringiensis subsp. morrisoni PG-14의 cryVID유전자를 가지고 있는 pCG10 플라스미드를 electroporation 방법을 이용하여 형질전환하였다. 형질전환체인 B. thuringiensis PT1227내에서 cryIVD와 숙주가 생성하는 원래의 다이아몬드형 130kDa 내독소 단백질 유전자는 그 자신의 형태로 잘 발현되었다. 형질전환체의 모기 유충에 대한 독성은 원래 숙주의 내 독소 단백질과ㅏ 도입된 CryIVD의 상승효과에 의해 현저히 증가하였다.

• 한국농공학회논문집
• /
• 제57권4호
• /
• pp.51-59
• /
• 2015

Various Best Management Practices (BMPs) have been suggested to reduce soil erosion and non point source (NPS) pollutant loads from agricultural fields. However, very little research regarding water quality improvement with No-till (NT) has been performed in Korea. Thus, effects of NT were investigated in this study. The objective of the study was to investigate the effect of NT on the surface runoff and sediment discharge in a field. Eight experimental plots of $5{\times}30m$ in size and 3 % or 8 % in slope prepared on gravelly sandy loam soil were treated with Conventional-till (CT) and NT. Runoff and NPS pollution discharge were monitored and compared the treatments. The amounts of rainfall from 13 monitored events ranged from 28.7 mm to 503.5 mm. The runoff amount was reduced by 17.6~59.2 % in 3 % NT and 29.6~53.2 % in 8 % NT. The average NPS pollution loads of the 3 % NT plots and 8 % NT plot were reduced about 45.1~89.2 % and 47.7~98.0 % compared to those of the CT plots, respectively. This research revealed that NT can reduce the NPS pollution loads substantially as well as increase the crop yield. Runoff and NPS pollution loads reduction by NT method could be contribute to improve the water quality of streams in agricultural regions.

• The Korean Journal of Physiology and Pharmacology
• /
• 제4권3호
• /
• pp.227-234
• /
• 2000

Many reports suggest that neurotensin (NT) in the gastrointestinal tract may play a possible role as a neurotransmitter, a circulating hormone, or a modulator of motor activity. NT exerts various actions in the intestine; it produces contractile and relaxant responses in intestinal smooth muscle. This study was designed to investigate the effect of NT on motility of antral circular muscle strips in guinea-pig stomach. To assess the role of $Ca^{2+}$ influx in underlying mechanism, slow waves were simultaneously recorded with spontaneous contractions using conventional intracellular microelectrode technique. At the concentration of $10^{-7}$ M, where NT showed maximum response, NT enhanced the magnitude $(863{\pm}198%,\;mean\;SEM,\;n=13)$ and the frequency $(154{\pm}10.3%,\;n=11)$ of spontaneous contractions. NT evoked a slight hyperpolarization of membrane potential, tall and steep slow waves with abortive spikes $(278{\pm}50%,\;n=4).$ These effects were not affected by atropine $(2\;{\mu}M),$ guanethidine $(2\;{\mu}M)$ and tetrodotoxin (0.2μM). NT-induced contractile responses were abolished in $Ca^{2+}-free$ solution and reduced greatly to near abolition by $10\;{\mu}M$ of verapamil or 0.2 mM of $CdCl_2.$ Verapamil attenuated the effects of NT on frequency and amplitude of the slow waves. Taken together, these results indicate that NT enhances contractility in guinea-pig gastric antral circular muscle and $Ca^{2+}$ influx through the voltage-operated $Ca^{2+}$ channel appears to play an important role in the NT-induced contractile mechanism.

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권4호
• /
• pp.349-355
• /
• 2015

We examined the effects of peripherally or centrally administered botulinum neurotoxin type A (BoNT-A) on orofacial inflammatory pain to evaluate the antinociceptive effect of BoNT-A and its underlying mechanisms. The experiments were carried out on male Sprague-Dawley rats. Subcutaneous (3 U/kg) or intracisternal (0.3 or 1 U/kg) administration of BoNT-A significantly inhibited the formalin-induced nociceptive response in the second phase. Both subcutaneous (1 or 3 U/kg) and intracisternal (0.3 or 1 U/kg) injection of BoNT-A increased the latency of head withdrawal response in the complete Freund's adjuvant (CFA)-treated rats. Intracisternal administration of N-methyl-D-aspartate (NMDA) evoked nociceptive behavior via the activation of trigeminal neurons, which was attenuated by the subcutaneous or intracisternal injection of BoNT-A. Intracisternal injection of NMDA up-regulated c-Fos expression in the trigeminal neurons of the medullary dorsal horn. Subcutaneous (3 U/kg) or intracisternal (1 U/kg) administration of BoNT-A significantly reduced the number of c-Fos immunoreactive neurons in the NMDA-treated rats. These results suggest that the central antinociceptive effects the peripherally or centrally administered BoNT-A are mediated by transcytosed BoNT-A or direct inhibition of trigeminal neurons. Our data suggest that central targets of BoNT-A might provide a new therapeutic tool for the treatment of orofacial chronic pain conditions.

• 한국정보기술응용학회:학술대회논문집
• /
• 한국정보기술응용학회 2007년도 춘계학술대회
• /
• pp.387-402
• /
• 2007

전체적으로 우리나라의 IT-BT-NT 융합기술에 대해 융합기술 개발이 미래의 성장동력 창출에 매우 중요함에도 불구하고 융합기술 개발면에서 IT - BT 는 선진국에 비해 $65{\sim}80%$, NT-BT는 $65{\sim}65%$, IT-NT는 $50{\sim}80%$로 열세를 보이고 있다. 그러므로 융합기술개발에 기반한 신산업 육성을 위해서는 먼저 정부측에서 연구개발 예산의 확대와 자금지원, 종합적인 융합기술개발전략의 수립, 관련부처간 역할분담과 조정, 법 제도 정비 및 역기능 방지대책의 마련이 필요하다.