• Archives of Pharmacal Research
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• v.20 no.5
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• pp.510-512
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• 1997

The effects of noscapine a phthalide isoquinoline alkaloid, on dopamine biosynthesis and tyrosine hydroxylase (TH) activity in PC12 cells were investigated. Noscapine showed 74.6% inhibition on dopamine content in PC12 cells at a concentration of $20{\mu}M.$ $IC_{50}$ of noscapine was $6.8{\mu}M.$ TH activity was inhibited by the treatment of noscapine in PC12 cells (20.9% inhibition at 20 .mu.M). Therefore, the inhibition of TH activity by noscapine might be involved in at least one component of the reduction of dopamine biosynthesis in PC12 cells.

• The Korean Journal of Physiology and Pharmacology
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• v.9 no.6
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• pp.323-326
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• 2005

In the present study, we investigated whether ambroxol, S-carboxymethyl-L-cysteine, dextromethorphan and noscapine affect mucin release from airway goblet cells. Confluent primary hamster tracheal surface epithelial cells were metabolically radiolabeled and chased for 30 min in the presence of varying concentrations of the above agents to assess the effects on $^3H$-mucin release. Noscapine stimulated mucin release during 30 min of treatment period in a dose-dependent manner. However, ambroxol, S-carboxymethyl-L-cysteine and dextromethorphan showed no significant effect on mucin release during 30 min of treatment period. We conclude that noscapine can affect mucin release by acting on airway mucin-secreting cells.

• YAKHAK HOEJI
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• v.32 no.4
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• pp.251-257
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• 1988

A simple and sensitive HPLC method was developed for the simultaneous determination of ten kinds of active ingredients formulated in commercial cough-cold mixtures. A group of Pseudoephedrine HCl, dl-Methylephedrine HCl, Noscapine, Chlorophenylamine maleate, Dextromethorphan HBr and Phenylpropanolamine HCl were determined at 254nm using a Novapak $C_{18}$ column with mobile phase consisting of a mixture of methanol-acetonitrile-1, 4dioxane-tetrahydrofuran-water(12 : 20 : 20 : 5 : 43, pH4.7) containing 0.013M-dioctyl sodium sulfosuccinate. The another group of Acetoaminophen, Caffeine, Guaifenesin and Ethenzamide were also determined at 254nm using a Novapak $C_{18}$ column as the stationary phase, and a mixture of methanol-1% aqueous acetic acid (3 : 7). The results indicate that these methods are accurate and precise with relative standard deviation of not more than 1% (n=5) for the above active ingredients.

• YAKHAK HOEJI
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• v.54 no.5
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• pp.416-421
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• 2010

We examined whether indomethacin, noscapine, coumarin, uridine and betaine affect airway mucin production induced by EGF or TNF-${\alpha}$ from NCI-H292 cells. Cells were pretreated with each agent for 30 min and then stimulated with EGF or TNF-${\alpha}$ for 24 h. Of the five compounds, coumarin suppressed airway mucin production induced by EGF or TNF-${\alpha}$. However, indomethacin suppressed airway mucin production induced by EGF. This result suggests that coumarin and indomethacin can regulate the production of mucin induced by EGF, by directly acting on airway epithelial cells.

• Korean Journal of Pharmacognosy
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• v.1 no.3
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• pp.93-99
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• 1970

There have been reported by several workers for the isolation and determination of the amine derivatives as Metbylephedrine Hydrochloride and Ephedrine Hydrochloride adopting neutralization method, steam distillation method, non-aqous titration method, ion-exchange resin method, titration method after acetylation, colorimetric method, gravimetric method, iodine titration method and gas chromatography. Those methods mentioned in above, can be practically applied for the sample which is not mixed one mith the other amine compounds. Presently, it has not shown on the isolative determination of the mixed sample of amine derivatives. In this paper, it is discussed on the isolative determination of Methylephedrine Hydrochloride as the tertiary amine compound and Ephedrine Hydrochloride as the secondary amine compound. According to the results of the experiment, it could be summarized as follows: 1. There is no time-variation on the color reaction of Methylephedrine Hydrochloride and Ephedrine Hydrochloride with the color reagent, bromcresolgreen. And Methylephedrine Hydrochloride and Ephedrine Hydrochloride, respectively, can be determined spectrophotometrically by means oft his color reaction. 2. For the isolation of Methylephedrine Hydrochloride and Ephedrine Hydrochloride from the mixed sample, Methylephedrine Hydrochloride can be eluted by chloroform, while Ephedrine Hydrochloride by the mixed solvent of chloroform and ethylalcohol (2:1), from the celite column adsorbed at pH6.4 followed by extraction with ether undersodium hydroxide alkali re action. 3. When the sample is mixed with quinine hydrochloride, dihydrocodeine bitartate, and noscapine, these mixed compounds can be eliminated by means of stram distillation. 4. When the sample is mixed with chlorpheniramine maleate, dextromethorphan hydrobromide and diphenhydramine hydrochloride, the mixed compounds can be eliminated by means of steam distillation and celite adsorption column chromatography, In conclusion, the isolative determination method for Methylephedrine Hydrochloride and Ephedrine Hydrochloride studied in this paper, indicates with the excellent reproducibility and accuracy.