Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitory Effects of Noscapine on Dopamine Biosynthesis in PC12 cells

  • Published : 1997.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of noscapine a phthalide isoquinoline alkaloid, on dopamine biosynthesis and tyrosine hydroxylase (TH) activity in PC12 cells were investigated. Noscapine showed 74.6% inhibition on dopamine content in PC12 cells at a concentration of $20{\mu}M.$ $IC_{50}$ of noscapine was $6.8{\mu}M.$ TH activity was inhibited by the treatment of noscapine in PC12 cells (20.9% inhibition at 20 .mu.M). Therefore, the inhibition of TH activity by noscapine might be involved in at least one component of the reduction of dopamine biosynthesis in PC12 cells.

Keywords

References

  1. Bull. W. H. O. v.40 Codeine and its alternative for pain and cough relief Eddy,N.B.;Friebel,H.;Hahn,K.J.;Halbach,H.
  2. Eur. J. Clin. Pharmacol v.16 Comparison of the antutussive effects of codeine phosphate 20 mg, dextromethorphan 30 mg and noscapine 30 mg using citric acid-induced cough in normal subjects Empey,D.W.;Laitinen,L.A.;Young,G.A.;Bye,C.E.;Hughes,D.T.D.
  3. J. Neurochem. v.30 Short-term regulation of catecholamine biosynthesis in a nerve growth factor responsive clonal line of rat pheochromocytoma cells Greene,L.A.;Rein,G.
  4. J. Biol. Chem. v.260 A new generation of $Ca^2+$, indicators with greatly improved fluorescence properties Grynkiewicz,G.;Poenie,M.;Tsien,R.Y.
  5. Planta Med. v.62 Inhibitory effects of protoberberine alkaloids from the the root of Coptis japonica on catecholamine biosynthesis in PC12 cells Lee,M.K.;Kim,H.S.
  6. Med. Sci. Res. v.24 Inhibition of tyrosine hydroxylase by berberine. Lee,M.K.;Zhang,Y.H.
  7. Arch. Pharm. Res. v.19 Inhibition of tyrosine hydroxylase by palmatine Lee,M.K.;Zhang,Y.H.;Kim,H.S.
  8. Proc. Natl. Acad. Sci. USA v.84 Transcriptional regulation of the tyrosine hydroxylase gene by glucocorticoid and cyclic AMP. Lewis,E.J.;Harrington,C.A.;Chikaraishi,D.M.
  9. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,R.J.
  10. J. Chromatogr v.344 High-performance liquid chromatography of plasma catecholamines using 1,2-diphenylethylenediamine as precolumn fluorescence derivatization reagent Mitsui,A.;Nohta,H.;Ohkura,Y.
  11. Mol. Pharmacol v.42 [³H]Noscapine binding sites in brain: Relationship to indolamines and the phosphoinositide and adenylyl cyclase messenger systems Mourey,R.J.;Dawson,T.M.;Barrow,R.K.;Enna,A.E.;Snyder,S.H.
  12. J. Chromatogr. v.163 Highly sensitive assay for tyrosine hydroxylase activity by high-performance liquid chromatography Nagatsu,T.;Oka,K.;Kato,K.
  13. Mutagenesis v.7 Morphological transformation of an established Syrian hamster dermal cell with the anti-tussive agent noscapine Porter,R.;Parry,E.M.;Parry,J.M.
  14. Mol. Brain Res. v.3 Regulation of tyrosine hydroxylase and phenolethanolamine N-methyltransferase mRNA levels in the sympathoadrenal system by the pituitaty-adrenocortical axis Stachowiak,M.K.;Rigual,R.J.;Lee,P.H.K.;Viveros,O.H.;Hong,J.S.
  15. J. Neurochem v.40 Glucocorticoids increase catecholamine synthesis and storage in PC12 pheochromocytoma cell cultures Tischler,A.S.;Perlman,R.L.;Morse,G.M.;Sheard,B.E.
  16. Planta Med. v.63 Inhibition of tyrosine hydroxylase by bulbocapnine Zhang,Y.H.;Shin,J.S.;Lee,S.S.;Kim,S.H.;Lee,M.K.