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Effects of Expander Processing and Enzyme Supplementation of Wheat-based Diets for Finishing Pigs

  • Park, J.S.;Kim, I.H.;Hancock, J.D.;Wyatt, C.L.;Behnke, K.C.;Kennedy, G.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.2
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    • pp.248-256
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    • 2003
  • Two experiments were conducted to determine the effects of expander processing and enzyme supplementation of wheat-based diets on growth performance and nutrient digestibility in finishing pigs. For Exp. 1, 60 finishing pigs (average initial BW of 49.5 kg) were fed meal, standard pellets and expanded pellets in a 70 d growth assay. From 49.5 to 79.0 kg, 79.0 to 111.8 kg, and overall (49.5 to 111.8 kg), ADG and ADFI were not affected by pelleting or standard vs expander conditioning (p>0.22). However, from 49.5 to 79.0 kg, pigs fed pellets have greater gain/feed than pigs fed mash (p<0.04), and pigs fed expanded pellets tended to have greater (p<0.10) gain/feed than pigs fed standard pellets. Overall (i.e. from 49.5 to 111.8 kg), gain/feed (p<0.02) and apparent fecal digestibilities of DM (p<0.001) and N (p<0.02) were improved by pelleting the diets. Also, expander processing further improved gain/feed (p<0.06) and digestibility of DM (p<0.04) compared to standard steam conditioning. Scores for keratinization (p<0.002) and ulceration (p<0.003) of the stomach were increased by pelleting, but the mean scores for the various treatments ranged only from 0.05 to 1.08 (i.e., low to mild keratosis and ulceration). For Exp. 2, 80 pigs (average initial BW of 54.1 kg) were fed mash and pellets (standard or expander) without and with xylanase. The enzyme was added to supply 4,000 units of xylanase activity/kg of diet. Adding xylanase to the mash diet improved gain/feed from 90.7 to 115.9 kg (p<0.04) of the growth assay and digestibility of DM (p<0.05) on d 39. However, in pelleted diets, adding the enzyme did not improve growth performance or digestibility of nutrients. Pelleting tended to increase scores for ulceration (p<0.06), and enzyme supplementation decreased stomach keratinization scores for pigs fed the standard pellets (p<0.01). However, as in Exp. 1, the mean scores for all treatment groups were quiet low (i.e., ranging from normal to mild). In conclusion, pelleting improved efficiency of growth, but additional benefits from expander conditioning were observed only in Exp. 1. Finally, xylanase tended to improve growth performance and nutrient digestibility, only in pigs fed mash diets but not in pigs fed pellets.

The Possible Mechanisms Involved in Citrinin Elimination by Cryptococcus podzolicus Y3 and the Effects of Extrinsic Factors on the Degradation of Citrinin

  • Zhang, Xiaoyun;Lin, Zhen;Apaliya, Maurice Tibiru;Gu, Xiangyu;Zheng, Xiangfeng;Zhao, Lina;Abdelhai, Mandour Haydar;Zhang, Hongyin;Hu, Weicheng
    • Journal of Microbiology and Biotechnology
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    • v.27 no.12
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    • pp.2119-2128
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    • 2017
  • Citrinin (CIT) is a toxic secondary metabolite produced by fungi belonging to the Penicillium, Aspergillus, and Monascus spp. This toxin has been detected in many agricultural products. In this study, a strain Y3 with the ability to eliminate CIT was screened and identified as Cryptococcus podzolicus, based on the sequence analysis of the internal transcribed spacer region. Neither uptake of CIT by cells nor adsorption by cell wall was involved in CIT elimination by Cryptococcus podzolicus Y3. The extracellular metabolites of Cryptococcus podzolicus Y3 stimulated by CIT or not showed no degradation for CIT. It indicated that CIT elimination was attributed to the degradation of intracellular enzyme(s). The degradation of CIT by C. podzolicus Y3 was dependent on the type of media, yeast concentration, temperature, pH, and initial concentration of CIT. Most of the CIT was degraded by C. podzolicus Y3 in NYDB medium at 42 h but not in PDB medium. The degradation rate of CIT was the highest (94%) when the concentration of C. podzolicus Y3 was $1{\times}10^8cells/ml$. The quantity of CIT degradation was highest at $28^{\circ}C$, and there was no degradation observed at 3$5^{\circ}C$. The study also showed that acidic condition (pH 4.0) was the most favorable for CIT degradation by C. podzolicus Y3. The degradation rate of CIT increased to 98% as the concentration of CIT was increased to $20{\mu}g/ml$. The toxicity of CIT degradation product(s) toward HEK293 was much lower than that of CIT.

Effects of physico-chemical treatment on 'Nunkeunhukchal' (black sticky rice with giant embryo) for the enhancement of GABA (γ- aminobutyric acid) contents ('눈큰흑찰'의 가바(GABA) 함량 증진을 위한 이화학적 처리 효과)

  • Han, Sang-Ik;Ra, Ji-Eun;Seo, Kyung-Hye;Park, Ji-Young;Seo, Woo Duck;Park, Dong-Soo;Cho, Jun-Hyun;Lee, Jong-Hee;Sim, Eun-Yeong;Nam, Min-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.4
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    • pp.398-405
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    • 2014
  • We assessed the GABA accumulation and other components after the 'Nunkeunhukchal (BGE)', 'Josanghukchal (BR)', and 'Ilmibyeo (IB)' grain was soaked in water for 24, 36, 48, 60, 72 and 96 hr. The results showed a continuous accumulation of GABA in soaking treated brown rice of BGE and IB. Among the treated hours, 72 hours of soaking had the maximal accumulation of GABA (51.4 mg/100 g), amino acid, polyphenol and other components. The activities of glutamate decarboxylase (GAD) in brown rice and rice-bran were the same in BGE rice. However, the formation of GABA treated with L-glutamate as substrate showed dramatic increase of 354.6 (fourteen times higher than normal extraction) and 726.4 mg/100 g in BGE rice and rice-bran, respectively. These results suggested that the soaking and extraction with L-glutamate buffer could be better methods for the harvest of increased GABA.

Nanophase Catalyst Layer for Direct Methanol Fuel Cells

  • Chang Hyuk;Kim Jirae
    • Journal of the Korean Electrochemical Society
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    • v.4 no.4
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    • pp.172-175
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    • 2001
  • Nanophase catalyst layer for direct methanol fuel cell has been fabricated by magnetron sputtering method. Catalyst metal targets and carbon were sputtered simultaneously on the Nafion membrane surface at abnormally higher gas (Ar/He mixture) pressure than that of normal thin film processing. They could be coated as a novel structure of catalyst layer containing porous PtRu or Pt and carbon particles both in nanometer range. Membrane electrode assembly made with this layer led to a reduction of the catalyst loading. At the catalyst loading of 1.5mg $PtRu/cm^2$ for anode and 1mg $Pt/cm^2$ for cathode, it could provide $45 mW/cm^2$ in the operation at 2 M methanol, 1 Bar Air at 80"C. It is more than $30\%$ increase of the power density performance at the same level of catalyst loading by conventional method. This was realized due to the ultra fine particle sizes and a large fraction of the atoms lie on the grain boundaries of nanophase catalyst layer and they played an important role of fast catalyst reaction kinetics and more efficient fuel path. Commercialization of direct methanol fuel cell for portable electronic devices is anticipated by the further development of such design.

Study for Fracture in the Last Stage Blade of a Low Pressure Turbine (화력발전용 저압터빈 최종 단 블레이드에 대한 파손 연구)

  • Lee, Gil Jae;Kim, Jae Hoon
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.4
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    • pp.423-428
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    • 2016
  • The last stage blades of a low pressure (LP) turbine get frequently fractured because of stress corrosion cracking. This is because they operate in a severe corrosive environment that is caused by the impurities dissolved in condensed steam and high stress due to high speed rotation. To improve the reliability of the blades under severe conditions, 12% Cr martensitic stainless steel, having excellent corrosion resistance and higher strength, is widely used as the blade material. This paper shows the result of root cause analysis on a blade which got fractured suddenly during normal operation. Testing of mechanical properties and microstructure examination were performed on the fractured blade and on a blade in sound condition. The results of testing of mechanical properties of the fractured blade showed that the hardness were higher but impact energy were lower, and were not meeting the criteria as per the material certificate specification. This result showed that the fractured blade became embrittled. The branch-type crack was found to have propagated through the grain boundary and components of chloride and sulfur were detected on the fractured surface. Based on these results, the root cause of fracture was confirmed to be stress corrosion cracking.

Grain Shattering Resistance and Its Screening Method of Sesame (참깨 내탈립성의 원인과 검정 방법)

  • Kim Dong-Hwi;Kang Chul-Whan;Park Chang-Hwan;Chae Young-Am;Seong Nak-Sul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.6
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    • pp.491-495
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    • 2004
  • The existing shattering-resistant sesames had low adaptability and yield potential in Korean environment. Great improvements have been made in these shattering-resistant sesames. We take an optimistic view of success for development of shattering-resistant sesames with high yield potential and superior agronomic characters. This study was carried out to investigate cause of shattering resistance and testing method of effective shattering habit. Shattering-resistant sesames had some specific tissue structures. Shattering resistance of placenta adhesion (PA) sesames was caused by strong seed holding of placenta in capsule, and that of seamless (SL) sesames was caused by nonexistence of seam in capsule. Shattering resistance of indehiscent(ID) sesames resulted because they had thicker mesocarp barrier at the zone of dehiscence compared with that of normal varieties. SL, ID and PA sesames had some variation plants who had high shattering rate. This was judged that evolution direction of these sesames means direction that shattering habit increase. Effective drying method in order to measure shattering resistance was drying condition over 20 days in natural temperature $(20^{\circ}C)$ and 10 days in drying oven $(40^{\circ}C)$.

Nutrients and Dish Intake by Fasting Blood Glucose Level (혈당 수준에 따른 영양섭취 및 음식섭취 상태)

  • Choi, Ji-Hyun;Moon, Hyun-Kyung
    • Journal of Nutrition and Health
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    • v.43 no.5
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    • pp.463-474
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    • 2010
  • The nutrient intake and association between dish group intake and blood glucose and serum lipid level (TG, cholesterol, LDL and HDL) was analyzed among 3 groups: 452 subjects in normal blood glucose group (NG: fasting blood glucose < 100 mg/dL and 2 hours postprandial blood glucose < 140 mg/dL), 258 subjects in impaired fasting glucose group (IFG: fasting blood glucose 100~125 mg/dL and 2 hours postprandial blood glucose ${\geq}$ 140 mg/dL) and 101 subjects in diabetic group (DG: fasting blood glucose $\geq$ 126 mg/dL and 2 hours postprandial blood glucose ${\geq}$ 140 mg/dL). The data were obtained from the 2005 National Health and Nutrition Survey of Korea. The 811 subjects were adults aged 40~64 without dietary treatment. In nutrients intake, IFG was the highest and DG the lowest in both quantity and quality. DG, especially, had the lowest intake in carbohydrates, fiber, proteins, Ca, P, K, vitamins B1 and C, and consumed the highest amount of alcohol. In macronutrients distribution ratio, the DG diet showed a lower energy intake from carbohydrates but higher from fat than the NG diet, while IFG showed a higher energy intake from carbohydrates and lower intake from fat in supper out of 3 meals and snacks. IFG preferred salt-fermented foods and DG preferred soups, braised foods and kimchi compared to other groups. NG preferred multi-grain cooked rice and both IFG and DG preferred plain white cooked rice. Regarding the association between dish group intake and blood glucose, cooked rice, soups, salt-fermented foods and kimchi were significantly related to blood glucose. In blood lipids, steamed-foods, beverages and fruits were inversely related to the risk of developing type 2 diabetes, whereas cooked rice, stews, saltfer-mented foods, seasoned-fermented foods and seasoned vegetables were directly proportional to the risk of developing type 2 diabetes and related diseases. Therefore, it is beneficial to avoid rich, salty and fatty foods and heavy alcohol consumption for controlling blood glucose and blood lipids, while steamed foods, foods rich in fiber (like multigrain rice) as a staple, and fruits and teas are recommended for preventing or managing type 2 diabetes risks.

Evaluation of Cold Tolerance-Related Traits of Recombinant Inbred Lines in Rice (벼 재조합 자식계통의 내냉성 관련 형질 분석)

  • Jeong Eung Gi;Ahn Sang Nag;Yea Jong Doo;Baek Man Kee;Choi Hae Chune;Yi Gihwan;Nam Min-Hee;Yoon Kyung Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.3
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    • pp.205-211
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    • 2005
  • This study was carried out to construct cold-tolerance characteristics. The RILs were developed from progenies of a cross between cold-susceptible Tongil-type rice variety, Milyang 23 and cold-tolerant Japonica rice variety, Stejaree 45 by single seed descent methods. The 175 RILs $(F_8)$ were evaluated for cold tolerance traits by field screening under cold-water irrigation. Frequency distribution of RILs in leaf discoloration, heading delay, culm length reduction and number of spikelets reduction displayed nearly normal distributions with transgressive segregations to either side of parents, while the spikelet fertility reduction and panicle exsertion at low-temperature showed the more or less skewed continuous distribution toward the susceptible parent. Higher heritabilities over $60\%$ were observed in leaf discoloration, spikelet fertility reduction, panicle exsertion, while relatively lower heritabilities less than $40\%$ were observed in culm length reduction, number of spikelets reduction and grain yield reduction. Some cold-tolerance RILs were selected effectively by cold water irrigation, which are expected to be good materials in breeding program for cold tolerance.

Improvement of Manila Clam (Ruditapes philippinarum) Habitat Condition by Adding Crushed Oyster (Crassostrea gigas) Shells to the Substratum (굴 패각을 이용한 바지락 양식장 저질개선 효과)

  • Park, Kwang-Jae;Yoon, Sang-Pil;Song, Jae-Hee;Han, Hyun-Seob;O, Hae-Chong
    • The Korean Journal of Malacology
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    • v.27 no.4
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    • pp.291-297
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    • 2011
  • In an attempt to improve the substrate condition for Manila clam (Ruditapes philippinarum) culture, crushed oyster (Crassostrea gigas) shells were spread on the muddy tidal flat of Namseong-ri, Podu-myeon, Goheung-gun, Jeollannam-do in April 2008. To test the suitability of the crushed oyster shell added substrate, seed clams were transplanted from Taehwa river estuary in Ulsan city in June 2008. Over 23 months of sampling, the mean grain size and the sorting in the experimental site containing the crushed oyster shell were significantly higher than the control site. The ignition loss, water content, chemical oxygen demand (COD) and acid-volatile sulfide (AVS) level were also significantly higher in the crushed oyste shell added substratum. Survival of the clams transplanted to the crushed oyster shell added substratum was significantly higher and all the clams transplanted to the normal muddy substratum died in August 2009, 13 months after the transplantation. At the end of the experiment in April 2010, the transplanted clams reached 36.10 mm in shell length and 8.92 g in total weight with survival of 43.5%. Our study suggested than crushed oyster shell added in the mud dominant substratum greatly improved living condition and survivability of clams.

Studies on the Engineering Characteristics of Alluvial Clayey Deposits in the Bay Area of Asan (II) (아산만지역 충적점토의 토질특성에 관한 연구(II))

  • 유능환;유연택
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.30 no.2
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    • pp.55-66
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    • 1988
  • This study was conducted to investigate the various engineering properties and correlationshops among the soil constants of alluvial clayey deposits distributed in the bay of Asan and their results are summarized as follows : 1. Grain size distribution of soil was consisted of 12 % of clay, 46-73 % of silt, 2-23 % of sand, and as for the consistency characteristics, 26-36 % of liquid limit, 18-21 % of plastic limit and 6-16 % of plastic index, and so the soil belonging to as a lower plastic nonorganic clay, it's specific gravity was 2,66-2.70, and the location on the plastic chart was approximately above the A-line. Z The natural moisture content and unit weight were 30-43 % and 1.76-1.87 g I cm$_3$, respectively, and according to increment of natural moisture content, the unit weight was decreased, and the initial void ratio and degree of saturation were shown of 0,87-1119 and 92- 100 %, most of saturated. 3. Cone resistance value which was shown 2.4 - 6.5 kg / $cm^2$ was a little lower and it was increased with the depth of layer and shown the formular $q_c=0.7_z+1.32$. 4. Unconfined compression strength was about 0.18-0.43kg /$cm^2$, cu, 0.1-0.22kg / $\psi$, $2-6^{\circ}$ under uu-test condition of triaxial, and CCU, 0.08-0.3 kg/cm , $\psi$, $12-18^{\circ}$ under the condition of cu-test. 5. Pre-consolidation load of characteristics of consolidation was 0.4-0.8 kg / $cm^2$, compression index, about 0.17-0.33. 6. Liquid limit and plastic index were incresased with the increment of clay content but most of alluvial clay was appeared as a normal through non-activity clay soil shown more natural moisture content than liquid limit, and their relationship as follows : LL=0.38( cy+54.8), PI=0.836(LL -17.8), PI =0.468(LL -0.48) 7. The initial void ratio presented correlationship of positive among clay content, natural moisture content and liquid limit, and that of reverse with unit weight, and their results as follws : $e_o=0.024(w_n+0.2)$, $e_o=e_o=0.0003c_y+0.0005 LL+0.0151 W_n+\frac{3.58}{r-t}-1.52$ 8. It was shown that the compression index has correlationship of postive among the clay content, liquid limit, plastic index, natural moisture content and initial void ratio, and their relationships as follows ; $c_c=0.44(e_o-0.47)$, $c_c=0.001$

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