• 한국약용작물학회지
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• 제19권5호
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• pp.319-324
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• 2011

This study was carried out to investigate the effect of Lythrum salicaria L. ethanol extract on anti-obesity effects in rat fed a high fat diet for 8 weeks to induce obese rat model. Male SD rats were divided into normal group, control (high fat diet) group, positive control (Garcinia Cambogia extracts) group, high fat group supplemented with ethanol extracts of Lythrum salicaria L. (EELS). The body weight gain and control (high fat diet) were increased by a high fat diet, but decreased in the EELS. At the end of the experiment, the body weight in high fat diet groups was higher than that of normal diet group, while the body weights of EELS and positive control group were significantly reduced by 16.62%, as compared with that of high fat diet group (p < 0.05). The levels of serum triglyceride, total cholesterol in EELS group were significantly decreased as compared with high fat diet group (p < 0.05). The liver and mesenteric adipose tissue weights of control (high fat diet) increase than that for normal group, whereas EELS and positive control group were significantly decreased (p < 0.05). Levels of triglyceride in liver were significantly lower in EELS group than those in high fat diet group (p < 0.05). These results indicate that Lythrum salicaria L. extract may improve lipid metabolism and reduce fat accumulation and body weight.

• Journal of Nutrition and Health
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• 제33권6호
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• pp.619-624
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• 2000

The purpose of this study was to investigate the effects of vitamin E on microsomal mixed function oxidase system of kidney in streptozotocin(STZ) induced diabetic rats. Sprague-Dawley male rats weighing 140$\pm$10g were randomly assigned to one control and three STZ-diabetic groups which were subdivided into vitamin E free diet(DM-0E group) 40mg vitamin E per kg diet(DM-40E group) and 400mg vitamin E per kg diet(DM-400E group). Vitamin E level of normal group was 40 mg per kg diet. Diabetes was experimentally induced by intravenous administration of 55 mg/kg B.W of STZ in citrate buffer(pH4.3) after 4 weeks feeding of experimental diets. Animals were sacrificed at the 6th day of diabetic state. The contents of cytochrome P450 in kidney were increased by 82, 54, 41% in DM-0E, DM-40E and DM-400E groups respectively when compared with normal group. The contents of cytochrome b5 in kidney were increased by 28% in DM-0E when compared with normal group but those of DM-40E and DM-400E groups were similar to that of normal group. The activities of NADPH-cytochrome P450 reductase in kidney that were increased by 35% in DM-0E group. Levels of TBARS(thiobarbituric acid reactive substance) in kidney were increased by 207, 129% and 72% in DM-0E and DM-400E groups respectively when compared with normal group but those of DM-40E and DM-400E groups were 26,44% lower than that of DM-0E groups. It is know that the activities of MFO system and lipid peroxidation were inhibited in kidney of STZ-induced diabetic rat by administeration of high doses of vitamin E.(Korean J Nutrition 33(6) : 619~624, 2000)

• 한국식품영양과학회지
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• 제26권5호
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• pp.901-907
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• 1997

The purpose of this study was to investigate the effects of green tea catechin o n free radical generation system and peroxidative damage in the liver of streptozotocin(STZ)-induced diabetic rats. Spragu-Dawley male rats weighing 150$\pm$10gm were randomly assigned to one normal and three STZ-induced diabetic groups; diabetic groups were classified to catechin free diet(DM-oC group), 0.5% catechin diet(DM-0.5C group) and 1% catechin diet(DM-1C group) according to the levels of dietary catechin supplementation. Diabetes was experimentally induced by intravenous injection of 55mg/kg of body wt of STZ in citrate buffer(pH 4.3) after feeding of three experimental diet for 4 weeks. Animals were sacrificed at the 6th day of diabetic states. Activities of serum glutamic oxaloacetic transaminase(GPT) in DM-oC groups were higher than those of the normal group, and those in catechin supplementation group were similar to those of the normal group. Liver lipid peroxide values increased by 153%, 49%, and 27% in Dm-oC, DM-0.5C and DM-0C and Dm-1C but was not significantly different in catechin supplementation groups compared with the normal group, and liver cytochrome $P_{450}$ contents was similar to result of XOD activity. In electron microscopic examination of liver, lysosome was relatively scattered in Dm-oC and Dm-0.5C group and preserved normal shapes in DM-1C group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stress, leading to the acceleration of lipid peroxidation process, but this was reduced by anti-oxidative effect of high level of dietary catechin. It is concluded that dietary catechin serves as powerful antioxidant against lipid peroxidation in diabetic rats.

• 한국환경과학회지
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• 제28권5호
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• pp.475-483
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• 2019

The purpose this study was to investigate the influences of 5% turmeric (Curcuma longa L.) supplementation on enzyme activities such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), amylase, lipase and catalase in serum of dyslipidemic rats. Sprague-Dawley (SD) rats (24 male) were divided into four groups, namely the ND group (normal-nondyslipidemic diet), NT group (normal-nondyslipidemic diet+5% turmeric), DD group (control-dyslipidemic diet), and DT groups (dyslipidemic diet+5% turmeric). Serum concentrations of blood urea nitrogen (BUN), creatinine and uric acid were significantly decreased (p<0.05) by turmeric supplementation diet. The activities of AST, ALT, ALP, LDH, amylase and lipase in sera of turmeric diet group were significantly decreased (p<0.05). The catalase activity in serum of turmeric supplementation group was significantly increased than dyslipidemic diet (p<0.05). In vivo experiment with dyslipidemic rats showed that ingestion of turmeric were effective in kidney and hepatic functional enzyme activities. Which suggests that turmeric material could be used for further studies as a potential source for nutraceutical foods.

• 치과방사선
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• 제22권2호
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• pp.223-234
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• 1992

The purpose of this study was to investigate the changes of periodontal tissues in the irradiated mandibular bone in rats which were fed normal diet and low calcium diet In order to carry out this experiment, 64 seven-week old Sprague-Dawley strain rats weighing about 150gms were selected and equally divided into one experimental group of 32 rats and one control group with the remainder. The experimental group and the control group were then subdivided into two groups when the rats reached the age of 10 weeks, 16 rats were allotted for each subdivided group was composed of 16 rats and exposed to irradiation. The two groups were irradiated a single dose of 20Gy on the only jaw area and irradiated with a cobalt-60 teletherapy unit The rats in the control and experimental groups were serially dissected by fours on the 3rd, the 7th, the 14th, and the 21st day after irradiation. After each dissection, both sides of the dead rat mandibular bodies were removed and fixed with 10% neutral formalin. The specimens sectioned and observed in histopathological. histochemical. and immunocellular chemical methods. The obtained results were as follows: 1. In the mandibles of rats with low calcium diet the increased number of fibroblasts of periodontal ligaments. many small capillaries and irregular arrangement of loose collagen fibers were detected and the partial resorption of dentin and cementum could be found by the microscopic studies. 2. In the group of irradiated rats, degenerated periodontal tissues led to the condition of irregular arrangement of collagen fibers and the decreased number of fibroblasts. But this condition was somewhat restored after 21 days of experiment. 3. Periodontal tissues of the irradiated rat group with low calcium diet were destroyed earlier than those of the irradiated rat group with normal diet. Soon this condition was restored and then high cellularity and dense collagen fibers were observed. 4. Many periodontal cells bearing tumor necrosis factor could be clearly observed in the nonirradiated group of rats with normal diet, whereas could not be observed on the 7th day and reappeared on 14th day in the irradiated group of rats with normal diet. A few of them could be observed in the group of rats with low calcium diet, but they could be clearly observed in the both groups after 21 days of experiment.

• 대한예방한의학회지
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• 제9권1호
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• pp.105-118
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• 2005

Objectives : The purpose of this study is to investigate the effects of Polygonum multiflorum THUNB(何首烏) on decresing concentration of serum lipid which are triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol. Thiobarbituric Acid Reactive(TBARS) in high cholesterol diet induced hyperlipidemia rats. Methods : Sprague-Dawley rats were divided into 3 groups, Normal group (supplied enough water and feeds only, Normal Group), high fat diet administered group(AIN-76 high fat diet #100496 used beef tallow for 4 weeks, Control Group) and Polygonum multiflorum THUNB(何首烏). administered group(AIN-76 high fat diet #100496 used beef tallow and Polygonum multiflorum THUNB(何首烏) extracts 85.0mg/200g 1time/day for 4 weeks, HSO Group). Rats were sacrificed and concentration of triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, TBARS were measured in rat's serum lipid. Results : There was no significant difference in the level of weigh between Control and HSO Group.(by Duncan test) Concentration of Total lipid has no significant difference in each group. (F=0.702, p=0.511, ANOVA test) Concentration of triglyceride has no significant difference in each group. (F=0.727, p=0.500, ANOVA test) Concentration of LDL-cholesterol was significantly different in each group.(F=.9.894. p=0.002, ANOVA test) It was significantly increased in the Control group compared with the Normal group and it significantly decreased in the HSO group compared with the Control group. Concentration of HDL-cholesterol has no significant difference in each group. (F=1.079, p=0.365, ANOVA test) TBARS values(Thiobarbituric Acid Reactive Substances) were significantly different in each group.(F=15.580, p=0.0001, ANOVA test) It was significantly increased in the Control group compared with the Normal group and significantly decreased in HSO group compared with the Control group. Conclusions : According to the above results, it is considered that Polygonum multiflorum THUNB(何首烏) is effective in decreasing concentration of serum lipid in high cholesterol diet induced hyperlipidemia rats.

• 대한한방내과학회지
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• 제19권1호
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• pp.364-372
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• 1998

It was investigated effect of Fructure schizandra chinesis Water extract on the white rat which was induced hypercholesterolemia by fatty diet. The results obtained were summarized as follows; Neutral lipid level in blood lipid was greatly decreased on the group being fed animal fat diet and Fructure schizandra chinesis Water extract, total cholesterol was decreased on the group being fed vegetative fat diet, HDL-cholesterol was shown that the group being fed vegetative fat diet and Fructure schizandra chinesis Water extract at a time more increased HDL-cholesterol in a little than the other group, and normal fat diet group show to highest figure,38.5mg/dl. Animal fat diet group is 20.75 mg/dl. but, the group being fed Fructure schizandra chinesis Water extract was increased to 31.00mg/dl. LDL-cholesterol had no difference between fat diet group and the group being fed Fructure schizandra chinesis Water extract.

• Imaging Science in Dentistry
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• 제30권3호
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• pp.169-181
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• 2000

Purpose: To investigate the expression of bone morphogenetic protein (BMP)-2/4 during eary tooth development after irradiation and calcium-deficient diet. Materials and Methods: The pregnant three-week-old Sprague-Dawley rats were used for the study. The control group was non-irradiation/normal diet group (Group 1), and the experimental groups were irradiation/normal diet group (Group 2) and irradiation/calcium-deficient diet group (Group 3). The abdomen of the rats at the 9th day of pregnancy were irradiated with single dose of 350 cGy. The rat pups were sacrificed at embryonic 18 days, 3 days and 14 days after delivery and the maxillae tooth germs were taken. The tissue sections of specimen were stained immunohisto-chemically with anti-BMP-2/4 antibody. Results: At embryo-18 days, immunoreacivity for BMP-2/4 of the Group 1 was modetate in stratum intermedium of dental organ and weak in dental papilla and dental follicle, but that of Group 2 was weak in cell layer of dental organ, and no immunoreacivity was shown in dental papilla and dental follice of Group 2 and in all tissue components of the Group 3. At postnatal-3 days, immunoreacivity for BMP-2/4 of the Group 1 was strong in cell layer of dental organ, odontoblasts and developing alveolar bone, but that of Group of 2 and Group 3 was weak in odontoblasts and developing alveolar bone. At postnatal-14 days, immunoreacivity for BMP-2/4 of the Group 1 was strong in newly formed cementum, alveolar bone and odontoblasts, but that of Group 2 was weaker than that of Group 1. In the Group 3, tooth forming cell layer showed weak immunoreactivity, but other cell layers showed no immunoreactivity. Couclusion : The expression of bone morphogenetic protein (BMP)-2/4 during early tooth development was disturbed after irradiation and calcium-deficient diet.

• 한국식생활문화학회지
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• 제17권4호
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• pp.377-386
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• 2002

The purpose of this study was to investigate the effects of dietary Indongcho(L. japonica Thunb) powder on blood glucose, serum lipid levels and antioxidative enzymes in normal and streptozotocin(STZ)-induced diabetic rats. Four groups of rats(3-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(NC), diabetic rats fed control diet(DC), normal rats fed Indongcho powder diet(NI), and diabetic rats fed Indongcho powder diet(DI). Diabetes was induced by single injection of streptozotocin(60mg/kg B.W., i.p.). The animals were fed ad libium each of the experimental diet for 5 weeks. Food and water intakes were determined everyday. Blood glucose and serum total cholesterol levels were determined every week. After 5 weeks the animals were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver and kidney homogenates. We also determined serum concentrations of total lipid(TL), total cholesterol(TC), triglycerides(TG) and HDL-cholesterol(HDL-C). Blood sugar and water intake were higher in diabetic group(DC and DI group) than normal group(NC and NI group) and were not significantly decreased by dietary Indongcho intake. Body weight gain and FER(feed efficiency ratio) were reduced by STZ treatment. But, Final body weight was recovered by Indongcho-contained diet. LHR(LDL-cholesterol/HDL-cholesterol) of the DI g re up was significantly lower than the other experimental groups(NC, NI and DC groups). The hepatic glucose 6-phosphatase(G6Pase) activity of the groups fed Indongcho diet(NI and DI group) was lower than the groups fed control diet(NC and DC group) and the G6Pase activity of NI group was recovered to the normal levels(p<0.05). However, The glutathione peroxidase(GPx) and glutathione reductase(GR) activities in liver and G6Pase activity in kidney were not statistically different between the control and diabetic control groups. Renal GST activity of the DI group was recovered by Indongcho intake. In conclusion, these results confirm oxidative stress in the liver and kidney of rats with STZ diabetes and antioxidative effect of Indongcho.

• 한국식품영양과학회지
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• 제42권10호
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• pp.1585-1591
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• 2013

본 연구의 목적은 고지방식으로 유도된 비만이 에너지 항상성 조절인자인 신경영양인자와 섭식조절 호르몬인 렙틴 mRNA 발현에 어떠한 관련성이 있는지를 규명하는 것과 비만 쥐의 시상하부 BDNF 발현과 렙틴 리셉터 mRNA 발현, 혈청 렙틴과 항비만과의 관계에 대한 운동트레이닝과 정상식이로의 전환에 대한 효과를 확인하고자 하였다. 그 결과 8주 후 비만 대조군(HF)에 비해 운동군(HFT), 정상식이군(HFND), 운동 및 정상식이 트레이닝군(HFNDT)에서 각각 유의한 체중 및 혈중 TC 감소를 보였다(P<0.05). 혈중 TG에서는 HF군에 비해 HFNDT군에서 유의한 감소를 보였다(P<0.05). 혈중 렙틴은 HF군에 비해 HFT군에서 유의한 감소를(P<0.05), HFT군에 비해 HFND군에서 유의한 감소를 보였으며(P<0.05), HFND군에 비해 HFNDT군에서 유의한 감소를 나타냈다(P<0.05). 시상하부 BDNF 단백질 발현은 HF군에 비해 모든 군에서 유의한 증가를 보였다(P<0.05). 시상하부 렙틴 리셉터 mRNA는 HF군에 비해 HFT와 HFND군에서 약간의 감소를, HFNDT군에서는 증가를 나타내었다. 이상의 결과는 고지방식이로 유도된 비만쥐에 대한 운동트레이닝은 항비만 효과가 있는 것으로 나타났으며, 운동 또는 정상식이의 병행은 식욕과 체중조절에 최적의 비만예방 및 개선효과가 있을 것으로 사료된다.