• Asian-Australasian Journal of Animal Sciences
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• v.33 no.2
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• pp.254-263
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• 2020

Objective: The objective of this study was to investigate the effects of wilting and additives on the fermentation quality, structural and non-structural carbohydrate composition of mulberry silages. Methods: The selected lactic acid bacteria strains Lactobacillus plantarum 'LC279063' (L1), commercial inoculant Gaofuji (GF), and Trichoderma viride cellulase (CE) were used as additives for silage preparation. Silage treatments were designed as control (CK), L1, GF, or CE under three wilting rates, that is wilting for 0, 2, or 4 hours (h). After ensiling for 30 days, the silages were analyzed for the chemical and fermentation characteristics. Results: The results showed that wilting had superior effects on increasing the non-structural carbohydrate concentration and degrading the structural carbohydrate. After ensiling for 30 days, L1 generally had a higher fermentation quality than other treatments, indicated by the lower pH value, acetic acid, propionic acid and ammonia nitrogen (NH₃-N) content, and the higher lactic acid, water soluble carbohydrate, glucose, galactose, sucrose, and cellobiose concentration (p<0.05) at any wilting rate. Wilting could increase the ratio of lactic acid/acetic acid and decrease the content of NH₃-N. Conclusion: The results confirmed that wilting degraded the structural carbohydrate and increased the non-structural carbohydrate; and L1 exhibited better properties in improving fermentation quality and maintaining a high non-structural carbohydrates composition compared with the other treatments.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2002.09a
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• pp.21-21
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• 2002

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.2
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• pp.142-147
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• 2006

The present study describes carbohydrate metabolism, macro-element utilization and antioxidant defenses in response to an ozone dose (100 ppb, 8d) in two rice varieties. Tolerant (cv. Jinpumbyeo) and sensitive (cv. Chucheongbyeo) varieties of rice were grown in growth chamber for 30 days after sowing. Concentrations of chloroplast pigments and non-structural carbohydrates as well as activity of antioxidant enzymes were determined to evaluate the resistance against ozone stress. Ozone caused the decrease in chlorophyll a and carotenoid contents, and also resulted in faster decomposition of non-structural carbohydrate in leaf blade and leaf sheath. The contents of nitrogen and potassium in leaves were visibly decreased in cv. Chucheongbyeo with an increase in ozone exposure, but not in cv. Jinpumbyeo. Enzymatic antioxidants against ROS in both varieties responded in the order of POD, SOD and CAT, and their capacity was stronger in cv. Jinpumbyeo.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.11
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• pp.1575-1589
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• 2017

Objective: This study was conducted to determine molecular structures related to carbohydrates and lipid in alfalfa hay cut at early bud, late bud and early flower and in the afternoon and next morning using Fourier transform infrared spectroscopy (FT/IR) and to determine their relationship with alfalfa hay nutrient profile and availability in ruminants. Methods: Chemical composition analysis, carbohydrate fractionation, in situ ruminal degradability, and DVE/OEB model were used to measure nutrient profile and availability of alfalfa hay. Univariate analysis, hierarchical cluster analysis (CLA) and principal components analysis (PCA) were conducted to identify FT/IR spectra differences. Results: The FT/IR non-structural carbohydrate (NSCHO) to total carbohydrates and NSCHO to structural carbohydrate ratios decreased (p<0.05), while lignin to NSCHO and lipid CH3 symmetric to CH2 symmetric ratios increased with advancing maturity (p<0.05). The FT/IR spectra related to structural carbohydrates, lignin and lipids were distinguished for alfalfa hay at three maturities by PCA and CLA, while FT/IR molecular structures related to carbohydrates and lipids were similar between alfalfa hay cut in the morning and afternoon when analyzed by PCA and CLA analysis. Positive correlations were found for FT/IR NSCHO to total carbohydrate and NSCHO to structural carbohydrate ratios with non-fiber carbohydrate (by wet chemistry), ruminal fast and intermediately degradable carbohydrate fractions and total ruminal degradability of carbohydrates and predicted intestinal nutrient availability in dairy cows ($r{\geq}0.60$; p<0.05) whereas FT/IR lignin to NSCHO and CH3 to CH2 symmetric stretching ratio had negative correlation with predicted ruminal and intestinal nutrient availability of alfalfa hay in dairy cows ($r{\geq}-0.60$; p<0.05). Conclusion: FT/IR carbohydrate and lipid molecular structures in alfalfa hay changed with advancing maturity from early bud to early flower, but not during the day, and these molecular structures correlated with predicted nutrient supply of alfalfa hay in ruminants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.157-163
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Dry matter, nitrogen and non-structural carbohydrate content of plants grown under $5^{\circ}C$ or $20^{\circ}C$ of culture temperature during 25 days were investigated. The dry matter content of leaves and roots were significantly reduced under $5^{\circ}C$ compared with $20^{\circ}C$culture condition. Comparing with the dry matter per plant under $20^{\circ}C$, those in leaves and roots under $5^{\circ}C$ decreased to 25% and 10%, respectively, after 25 days of temperature treatment. Total nitrogen content in leaves under $20^{\circ}C$ and $5^{\circ}C$ increased to 68% and 39% compared to the initial lenel(day O), respectively, during 25 days after temperature treatment, Nitrogen content in roots highly increased under 5 C while there was a little change under $20^{\circ}C$ condition. The nitrogen contents in roots under $5^{\circ}C$ and $20^{\circ}C$ were 39.0 and 30.8mgJg DM, respectively, after 25 days of temperature treatment. Total contents of soluble carbohydrate in both leaves and roots under $5^{\circ}C$ were higher than those under $20^{\circ}C$ condition. After 25 days of temperature treatment under$5^{\circ}C$ , their contents in leaves and roots were 1.4 and 2.0 times higher than those of under $20^{\circ}C$ condition. Stach atent in roots under $20^{\circ}C$ was less changed, while thatof under $5^{\circ}C$ greatly increased from 64.8 to 178.7mglg DM duling 25 days. 'Ihese results clearly showed that an accumulation of both nitrogen and non-structural carbohydrate in the plants occured under low temperature condition.e condition.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.6
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• pp.859-863
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• 2018

Objective: Since very little information exists about the topic; in this experiment we compare, in a long-term finishing program, the growth-performance responses and carcass characteristics of Holstein steers where non-structural carbohydrate concentration of the diet is reduced from 64% to 51% (dry matter basis). Methods: Sixty Holstein steer calves ($129{\pm}2.2kg$) were blocked by initial weight into five groups and randomly assigned within weight groupings to 10 pens. Calves were fed with a steam-flaked corn-based finishing diets containing 51% higher fiber (HF) or 64% lower fiber (LF) nonstructural carbohydrates. Non-structural carbohydrates concentrations were manipulated substituting dried distiller grain with solubles and alfalfa hay for flaked corn. Cattle were weighed every 112 days and at the end of the experiment (day 308) when the cattle were harvested and carcass characteristics were evaluated. Results: Steers fed the HF diet showed improvement (8.8%) in average daily gain (ADG) during the initial 112-d period. This effect was followed by a numerical trend for greater ADG throughout the remainder of the study so that overall ADG tended to be greater (4.9%, p = 0.06) for the HF than for LF. There were no treatment effects on dry matter intake. Gain efficiency and estimated dietary net energy (NE) were greater 8.3% and 5.2%, respectively for HF during the initial 112-d period. Overall (308-d) gain efficiency and estimated dietary NE were similar for both dietary treatments. However, due to differences in tabular dietary NE, the ratio of observed:expected dietary NE tended to be greater (4.1%, p = 0.06) for the HF vs LF diet. There were no treatment effects on carcass characteristics except for a tendency toward a slightly greater (0.5%, p = 0.09) estimated carcass yield. Conclusion: Reducing the non-structural carbohydrate concentration of a conventional steam-flaked corn-based growing finishing diet for Holstein steers can effectively enhance growth performance, particularly during the early growing and late finishing phases.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.10
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• pp.1399-1406
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• 2000

A continuous culture study was conducted to determine the impact of ruminal degradable soy protein (S-RDP) level and dilution rate (D) on growth of ruminal non-structural carbohydrate-fermenting microbes. Corn starch, urea and isolated soy protein (ISP) were used to formulate three diets with S-RDP levels of 0, 35 and 70% of total dietary CP. Two Ds were 0.03 and $0.06h^{-1}$ of the fermenter volume in a single-effluent continuous culture system. As S-RDP levels increased, digestibilities of dietary dry matter (DM), organic matter (OM) and crude protein (CP) linearly (p=0.001) decreased, whereas digestion of dietary starch linearly (p=0.001) increased. Increasing D from 0.03 to $0.06h^{-1}$ resulted in decreased digestibilities of dietary DM and OM, but had no effect on digestibilities of dietary starch (p=0.77) and CP (p=0.103). Fermenter pH, the concentration of volatile fatty acids (VFA) and daily VFA production were unaffected (p=0.159-0.517) by S-RDP levels. Molar percentages of acetate, propionate and butyrate were greatly affected by S-RDP levels (p=0.016-0.091), but unaffected by D (p=0.331-0.442). With increasing S-RDP levels and D, daily bacterial counts, daily microbial N production (DMNP) and microbial efficiency (MOEFF; grams of microbial N produced per kilogram of OM truly digested) were enhanced (p=0.001). The increased microbial efficiency with increasing S-RDP levels is probably the result of peptides or amino acids that served as a stimulus for optimal protein synthesis. The quantity of ruminal degradable protein from soy proteins required for optimum protein synthesis of non-structural carbohydrate-fermenting microbes appears to be equivalent to 9.5% of dietary fermented OM.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.259-264
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• 2001

To investigate the physiological responses to naturally occurring winter freezing stress in creeping bentgrass, changes in carbohydrates were monitored during winter period. Turf quality and leaf growth was nearly parallel with temperature fluctuation. The concentration of glucose, fructose and sucrose in both shoot and root gradually increased from November to January, and then sharply decreased until April. Sucrose was the largest pool of soluble sugars. Fructan also slightly accumulated in both shoot and roots from November to February. Fructan hydrolysis in both organs was found to be much active between February to April. Shoot contained largely higher carbohydrate content in all compounds examined than roots did. Fructan was found to be a main carbohydrate storage form, showing the highest concentration (176.7 and 126.7 mg g-' DW for shoot and root in February). The depolymerization of fructan from February coincided with the high declines in mono- and disaccharide. These results suggest that the accumulation of non-structural carbohydrate until January could be associated with freezing tolerance, and the active decrease from February with shoot regrowth.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.11a
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• pp.137-138
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• 2000

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.5
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• pp.542-550
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• 1996

An experiment with alfalfa (Medicago sativa L.) plants was designed to investigate the changes in non-structural carbohydrate (NSC) contents and the activities of amylolytic enzymes during a regrowth period following defoliation. Sampling from hydroponic grown-plants were carried out at intervals during 24 days of regrowth. Shoot regrowth was very slow during the first 10 days and root growth was depressed after defoliation. Defoliation induced a great decrease in both total sugar and starch contents in taproots during the first 10∼14 days. A major recovery of NSC occurred from day 15. Averaged over sampling dates, the activity of exo-amylase was about 400-fold higher than that of endo-amylase. Exo-amylase activity in defoliate plants slightly increased until day 6 (maximum level) and then decreased. Endo-amylase rapidly increased for the first 4 days after defoliation and slightly increased afterwards to a maximum on day 24. These results showed that increase in amylolytic enzyme activity in taproots coincided with the time of starch utilization during regrowth and that indicated it plays an important role in starch degradation.