• Title/Summary/Keyword: Non-selective herbicide

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Genetic Transformation of a Mushroom Forming Fungus Coprinellus congregatus to an Antibiotic Resistance Using Oidia Instead of Protoplast Generation (먹물버섯 Coprinellus congregatus에서 분열자를 사용한 형질전환)

  • Park, Nam-Mee;Kim, Dong-Sik;Choi, Hyoung-T.
    • Korean Journal of Microbiology
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    • v.42 no.1
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    • pp.59-61
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    • 2006
  • Genetic transformation of a mushroom-forming fungus Coprinellus congregatus to antibiotic resistance gene had been successfully carried out by electroporation to oidia instead of protoplasts. Since there was no protoplast generation step which required not only cell wall degrading enzymes but many skillful procedures, commercial herbicide (basta) could be used without any difficulty with simple procedure. The transformation yield was 10-20 $transformants/{\mu}g$ DNA, and the transformants were very stable even after 10 consecutive transfers through the non-selective medium.

Weeding Hypothesis on Direct Seeding Rice Field as Applied by the Old Firing and Water Dressing Method (고대 화경수누법(火耕水?法)을 응용한 직파 논[直播畓] 제초법)

  • Guh, Ja-Ock;Kuk, Yong-In
    • Korean Journal of Weed Science
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    • v.31 no.1
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    • pp.1-7
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    • 2011
  • It was a method of weed removing, called as whagyeongsoonoobeob. Namely, the Hwanoobeob was the weeding method came from "Jeminyosool" in old China, and had been descended to "Wibinmyeongnonggi", "Nonggajibseong" and "Gwanongsocho" in the $16\sim18^{th}$ century of our country. The method was applied to rice paddy after applying direct-seeding, burning by unit before watering to remove weeds and to save rice plants. It would be applied with a method of using non-selective herbicide such as paraquat and oxyfluorfen, or radiation treatment, as a simple and safe weed removing method.

Selection of Herbicide Resistant Potatoes Transformed with Phosphinothricin Acetyltransferase Gene (Phosphinothricin Acetyltransferase 유전자 도입에 의한 제초제 저항성 감자의 선발)

  • Han, S.S.;Jeong, J.H.;Bang, K.S.;Yang, D.C.
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.390-399
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    • 1997
  • This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

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Herbicidal Phytotoxicity under Adverse Environments and Countermeasures (불량환경하(不良環境下)에서의 제초제(除草劑) 약해(藥害)와 경감기술(輕減技術))

  • Kwon, Y.W.;Hwang, H.S.;Kang, B.H.
    • Korean Journal of Weed Science
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    • v.13 no.4
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    • pp.210-233
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    • 1993
  • The herbicide has become indispensable as much as nitrogen fertilizer in Korean agriculture from 1970 onwards. It is estimated that in 1991 more than 40 herbicides were registered for rice crop and treated to an area 1.41 times the rice acreage ; more than 30 herbicides were registered for field crops and treated to 89% of the crop area ; the treatment acreage of 3 non-selective foliar-applied herbicides reached 2,555 thousand hectares. During the last 25 years herbicides have benefited the Korean farmers substantially in labor, cost and time of farming. Any herbicide which causes crop injury in ordinary uses is not allowed to register in most country. Herbicides, however, can cause crop injury more or less when they are misused, abused or used under adverse environments. The herbicide use more than 100% of crop acreage means an increased probability of which herbicides are used wrong or under adverse situation. This is true as evidenced by that about 25% of farmers have experienced the herbicide caused crop injury more than once during last 10 years on authors' nationwide surveys in 1992 and 1993 ; one-half of the injury incidences were with crop yield loss greater than 10%. Crop injury caused by herbicide had not occurred to a serious extent in the 1960s when the herbicides fewer than 5 were used by farmers to the field less than 12% of total acreage. Farmers ascribed about 53% of the herbicidal injury incidences at their fields to their misuses such as overdose, careless or improper application, off-time application or wrong choice of the herbicide, etc. While 47% of the incidences were mainly due to adverse natural conditions. Such misuses can be reduced to a minimum through enhanced education/extension services for right uses and, although undesirable, increased farmers' experiences of phytotoxicity. The most difficult primary problem arises from lack of countermeasures for farmers to cope with various adverse environmental conditions. At present almost all the herbicides have"Do not use!" instructions on label to avoid crop injury under adverse environments. These "Do not use!" situations Include sandy, highly percolating, or infertile soils, cool water gushing paddy, poorly draining paddy, terraced paddy, too wet or dry soils, days of abnormally cool or high air temperature, etc. Meanwhile, the cultivated lands are under poor conditions : the average organic matter content ranges 2.5 to 2.8% in paddy soil and 2.0 to 2.6% in upland soil ; the canon exchange capacity ranges 8 to 12 m.e. ; approximately 43% of paddy and 56% of upland are of sandy to sandy gravel soil ; only 42% of paddy and 16% of upland fields are on flat land. The present situation would mean that about 40 to 50% of soil applied herbicides are used on the field where the label instructs "Do not use!". Yet no positive effort has been made for 25 years long by government or companies to develop countermeasures. It is a really sophisticated social problem. In the 1960s and 1970s a subside program to incoporate hillside red clayish soil into sandy paddy as well as campaign for increased application of compost to the field had been operating. Yet majority of the sandy soils remains sandy and the program and campaign had been stopped. With regard to this sandy soil problem the authors have developed a method of "split application of a herbicide onto sandy soil field". A model case study has been carried out with success and is introduced with key procedure in this paper. Climate is variable in its nature. Among the climatic components sudden fall or rise in temperature is hardly avoidable for a crop plant. Our spring air temperature fluctuates so much ; for example, the daily mean air temperature of Inchon city varied from 6.31 to $16.81^{\circ}C$ on April 20, early seeding time of crops, within${\times}$2Sd range of 30 year records. Seeding early in season means an increased liability to phytotoxicity, and this will be more evident in direct water-seeding of rice. About 20% of farmers depend on the cold underground-water pumped for rice irrigation. If the well is deep over 70m, the fresh water may be about $10^{\circ}C$ cold. The water should be warmed to about $20^{\circ}C$ before irrigation. This is not so practiced well by farmers. In addition to the forementioned adverse conditions there exist many other aspects to be amended. Among them the worst for liquid spray type herbicides is almost total lacking in proper knowledge of nozzle types and concern with even spray by the administrative, rural extension officers, company and farmers. Even not available in the market are the nozzles and sprayers appropriate for herbicides spray. Most people perceive all the pesticide sprayers same and concern much with the speed and easiness of spray, not with correct spray. There exist many points to be improved to minimize herbicidal phytotoxicity in Korea and many ways to achieve the goal. First of all it is suggested that 1) the present evaluation of a new herbicide at standard and double doses in registration trials is to be an evaluation for standard, double and triple doses to exploit the response slope in making decision for approval and recommendation of different dose for different situation on label, 2) the government is to recognize the facts and nature of the present problem to correct the present misperceptions and to develop an appropriate national program for improvement of soil conditions, spray equipment, extention manpower and services, 3) the researchers are to enhance researches on the countermeasures and 4) the herbicide makers/dealers are to correct their misperceptions and policy for sales, to develop database on the detailed use conditions of consumer one by one and to serve the consumers with direct counsel based on the database.

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Weed control efficacy of the tank-mixture between quizalofop-P-ethyl and KSC-35089, a newly synthesized N-arylphthalimide compound (신규합성된 N-arylphthalimide 화합물 KSC-35089와 Quizalofop-ethyl의 혼합처리효과)

  • Kim, Tae-Joon;Song, Jae-Eun;Choi, Jung-Sup;Kim, Hyoung-Rae;Pyon, Jong-Young;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
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    • v.6 no.3
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    • pp.183-192
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    • 2002
  • Herbicidal effect of KSC-35089, a newly synthesized N-arylphthalimide compound, was examined, and its interaction with quizalofop-P-ethyl when foliar applied as a tank-mixture was evaluated under a greenhouse condition. KSC-35089 showed stronger herbicidal activity on broadleaves than grasses, suggesting that it would be an appropriate partner for non-selective weed control with quizalofop-P-ethyl, a strong grasskilling ageant. In general, the interaction between KSC-35089 and quizalofop-P-ethyl appeared to be additive, which indicates each compound acts on whole plant level independently. This additive interaction was confirmed in a spectrum test using 19 grasses and 26 broadleaves. Under the greenhouse condition, all tested plants were controlled around 90% level through a foliar application with a tank-mixture of KSC-35089 at 64 g ai/ha and quizalofop-P-ethyl at 32 g ai/ha. Further study would be required on the action mechanism of this additive interaction.

Weedy Rice Control by No-tillage Direct Seeding on Flooded Paddy Field (무경운 담수표면산파에 의한 잡초성벼 방제)

  • 정남진;윤영환;김정곤;강양순
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.3
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    • pp.195-198
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    • 2000
  • Weedy rice has typical characters such as easy shattering, dormancy, and longevity. These characters let it undergo the winter and occur year by year, It is very difficult to control weedy rice once the field have contaminated with its seeds. Therefore, the control method for weedy rice was focused on the no-tillage direct seeding in this experiment. The germination ability of seeds shattered on the soil surface in the next spring was 92.7% in weedy rice, and 4.3% in cultivated rice. The possible depth of weedy rice emergence were 1.5cm, 3.0cm and 6.8cm in no-tillage, wet seeding and dry seeding paddy field, respectively. The paddy field contaminated with weedy rice was maintained as no-tillage, and then irrigated in early spring (April 15). We could induce weedy rice on the soil to emerge with irrigation, and then kill by using non-selective herbicide, paraquat. Weedy rice was controled 92.2% of total emerged by this method. After seedling establishment of cultivated rice, molinate, thiobencarb, oxadiazon, dithiopyr, butachlor were soil-applied to suppress the emergence of weedy rice seeds buried in the soil. Oxadiazon was the most effective to repress the weedy rice among soil-applied herbicides tested. The highest control value was 96.4% as the result of combination of paraquat and oxadiazon.

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Genoprotective Effect of Melatonin Against to the Genotoxicity of Glyphosate on Human Blood Lymphocytes (글라이포세이트의 유전자 독성에 대한 멜라토닌의 유전자 보호 효과)

  • Kim, Jung-Gyu;Choi, Woo-Ik;Lee, Jae-Ho;Choi, In-Jang;Jin, Sang-Chan
    • Journal of The Korean Society of Clinical Toxicology
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    • v.14 no.2
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    • pp.144-150
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    • 2016
  • Purpose: Glyphosate is a widely used non-selective herbicide. Previous studies have shown that glyphosate has genotoxicity, and that even low-doses of glyphosate can cause DNA damage. Melatonin is a hormone produced and secreted by the pineal gland that is known to be a potent anti-carcinogen, anti-oxidant, and genetic protector. This study was conducted to investigate the genoprotective effect of melatonin against glyphosate in human blood lymphocytes. Methods: Human peripheral blood was obtained from 15 young, healthy volunteers and cultured under four different toxicologic conditions. The four groups consisted of a control group, glyphosate only group (300 ng/mL), glyphosate with low level of melatonin group ($50{\mu}M$), and glyphosate with high level of melatonin group ($200{\mu}M$). The mean Sister Chromatid Exchange (SCE) frequency of each group was then analyzed. Results: Glyphosate exposed groups had a higher mean SCE frequency ($10.33{\pm}2.50$) than the control group ($6.78{\pm}2.31$, p<0.001). Interestingly, the group that received a low-level of melatonin had a lower mean SCE frequency ($8.67{\pm}2.58$) than the glyphosate-only group, while the group that received a high level of melatonin had a much lower mean SCE frequency ($8.06{\pm}2.50$) than the glyphosate-only group. There was statistical significance. Conclusion: Melatonin exerted a potent gene protective effect against the genotoxicity of glyphosate on human blood lymphocytes in a dose-dependent fashion.

In vitro Effects of Epigallocatechin Gallate on Sister Chromatid Exchange in the Lymphocytes Exposed to Glyphosate (글라이포세이트 노출로 인한 DNA손상에 대한 녹차의 예방적 효과)

  • Park, Jung-Min;Choi, Woo-Ik;Jin, Sang-Chan;Lee, Jae-Ho;Choi, In-Jang
    • Journal of The Korean Society of Clinical Toxicology
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    • v.14 no.2
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    • pp.78-82
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    • 2016
  • Purpose: Green tea is known as a potent anti-oxidant, anti-carcinogen, and genetic protector. Glyphosate (N-phosphonomethyl glycine) is a widely used non-selective herbicide that causes DNA damage. The present study was conducted to investigate the protective effects of green tea in human blood lymphocytes exposed to glyphosate using the Sister Chromatid Exchange (SCE) frequency method. Methods: Peripheral blood was obtained from 10 volunteers and cultured through four different conditions. Four groups were divided into control, glyphosate only (300 ng/mL), glyphosate and low ($20{\mu}m$) concentrations of epigallocatechin gallate (EGCG) and glyphosate and high ($100{\mu}m$) concentrations of EGCG. Results: The glyphosate exposed groups had a higher mean SCE frequency ($10.33{\pm}2.50$) than the control group ($6.38{\pm}2.28$, p<0.001). The low concentrations of EGCG groups had a lower mean SCE frequency ($9.91{\pm}1.93$) than the glyphosate-only group, although this difference was not significant (p=0.219). However, the high concentration group ($9.49{\pm}1.85$) had a significantly lower SCE frequency than the glyphosate-only group (p=0.001). Conclusion: EGCG has a gene protective effect in human lymphocytes exposed to the genotoxicity of glyphosate in the case of high concentrations.

Antioxidant Enzyme Responses against Abiotic and Biotic Stresses in Rehmannia glutinosa L. and Glycine max L.

  • Moon, Yu-Ran;Lim, Jeong-Hyun;Park, Myoung-Ryoul;Yu, Chang-Yeon;Chung, Ill-Min;Yang, Deok-Chun;Yun, Song-Joong
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.360-365
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    • 2004
  • Rehmannia glutinosa shows a high level of resistance to the non-selective herbicide paraquat. To characterize the antioxidant enzyme system of R. glutinosa, we comparatively examined the responses of antioxidant enzymes to UV, wounding and a general elicitor yeast extract in R. glutinosa and soybean. The levels of enzyme activities of the two plant species were drastically different between those per fresh weight (general activity) and per protein (specific activity) bases. The general activities of superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), and glutathione reductase (GR) were lower, but that of ascorbate peroxidase (APX) was higher in R. glutinosa than in soybean. The specific activities of the enzymes, however, were about two- to seven-fold higher in R. glutinosa than in soybean, except that of CAT, which was about 12-fold higher in soybean. The general and specific enzyme activities of R. glutinosa relative to those of soybean showed a consistent increase in responses to the stresses only in SOD. The specific activities of SOD and APX were higher in R. glutinosa in all stress treatments. The results might suggest a relatively higher contribution of SOD and APX to the stress tolerance.

Major Weeds Occurring in Orchard and Their Effective Control by Oxyfluorfen (과수원(果樹園)의 주요잡초(主要雜草) 및 Oxyfluorfen 의 방제효과(防除効果))

  • Kim, K.U.;Pyon, J.Y.;Guh, J.O.;Shin, D.H.
    • Korean Journal of Weed Science
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    • v.2 no.1
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    • pp.57-62
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    • 1982
  • This study was conducted to evaluate the important weed occurring in orchard and to establish on effective control method using combination of non-selective foliar applied herbicide followed by soil treated herbicide. In terms of quantity, Calysregia hederacea, Artemisia princeps, Ixeris chinensis, Cephalonopios segetum, Capsella bursa-pastoris, Setaria viridis, Chenopodium album, Trifolium repens, Digitarta sanguinalis in order were observed to be the most important weeds in May, In July the most dominant weeds were D. sanguinalis, Setaria viridis, Amaranthus lividus, Portulaca oleracea, Polygonum hydropiper, Acalypha australis, Artemlsia prtnceps, Miscanthus sinensis in order, showing D. sanguinalis to be the dominant species. D. sanguinalis was one single dominant species till autumn, followed by A. lividus, P. oleracea, A. australis. Simpson's index, 0.159 obtained in May just before herbicide application increased to 0.253 in July and again decreased to 0.233 in Sept. in the oxyfluorfen treated plot, meaning that no specific weed species were dominant, while in the untreated plot, D. saguinalis was relatively single dominant species, showing Simpson's index, 0.540 and this trend was mainained till autumn. The most effective herbicide to control D. sanguinalis and other annuals was oxyfluorfen at 0.47kg a.i./ha. when applied at preemergence treatment, showing more than 80% control of them for 65 days. The results suggest that paraquat at 32/ha (prod.) as the foliar application followed by oxyfluorfen 0.47kg a.i./ha applied as the preemergence at one week after paraquat treatment can maintain the clean orchard more than 65 days, showing one of the promising and applicable control system in orchard.

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