• ALGAE
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• 제32권1호
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• pp.57-66
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• 2017

The red alga Gracilaria vermiculophylla, a species native to the waters of Korea and Japan, has invaded marine coastal areas of Europe and the Americas, thriving in conditions that differ from those of its native habitat. In recent years, G. vermiculophylla has been discovered in the Long Island Sound (LIS) estuary growing alongside the native congener Gracilaria tikvahiae. The goal of this study was to determine whether the two strains of G. vermiculophylla from different regions of the world have evolved genetic differences (i.e., ecotypic differentiation) or if the physiological performance of the strains simply reflects phenotypic plasticity. Two strains of G. vermiculophylla (isolated in Korea and LIS) and a strain of the LIS native G. tikvahiae were grown for four weeks under temperatures ranging from 20 to $34^{\circ}C$ using a temperature gradient table (all other environmental conditions were kept constant). At the end of each week, wet weight of each sample was recorded, and thalli were reduced to the original stocking density of $1gL^{-1}$ (excess biomass was preserved for tissue carbon and nitrogen analysis). Generally, the growth rates of Korean G. vermiculophylla > LIS G. vermiculophylla > G. tikvahiae. After one week of growth G. tikvahiae grew 9.1, 12.0, 9.4, and 0.2% $d^{-1}$, at temperatures of 20, 24, 29, and $34^{\circ}C$, respectively, while G. vermiculophylla (LIS) grew 6.6, 6.2, 5.7, and 3.6% $d^{-1}$. G. vermiculophylla (Korea) grew 15.4, 22.9, 23.2, and 10.1% $d^{-1}$, much higher than the two strains currently inhabiting the LIS. On average, the LIS G. vermiculophylla strain contained 4-5% DW N, while the Korean strain and G. tikvahiae had more modest levels of 2-3% N DW. However, tissue N content declined as temperature increased in LIS and Korean G. vermiculophylla. The non-native haplotype may have evolved genetic differences resulting in lower growth capacity while concentrating significantly more nitrogen, giving the non-native a competitive advantage.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.23-23
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• 2018

Taiwan, formerly known as Formosa, located on tropical and subtropical climate zones with abundant biological resources. According to the latest version of the Flora of Taiwan, there are 4339 species of vascular plants including 1054 endemic species. First, Taiwania (Taiwania cryptomerioides), named after its native island of Taiwan, have been isolated more than 500 secondary metabolites, including lignans, terpenoids, steroids, and flavonoids. Several of the metabolites are reported to have antibacterial, antifungal, antimite, antitermite and antitumor activities. In order to investigate plant secondary metabolic diversity toward industrial applications, we established deep transcriptome resources for non-model plants and fungi to produce terpenoid metabolites of economic importance. Second, many plants of Lauraceae have been utilized in folk medicine for their exciting bioactivities. The twigs and leaves from 27 tree species of Lauraceae grown in Taiwan were performed to evaluate potential bioactivity. The leaves of Cinnamomum osmophloeum are traditionally used in folk medicines, and many biological activities have been identified, such as antibacterial, antifungal, antitermite, antidiabetic, antihyperuricemia, antiinflammatory, and antioxidant activities. However, C. osmophloeum has nine chemotypes with various secondary metabolite profiles. In order to efficiently produce active compounds, we established the genetic markers to identify the chemotype plants. Finally, Cinnamomum kanehirae is the host of the medicinal mushroom Antrodia cinnamomea. Several in vivo and in vitro studies indicated that A. cinnamomea possesses a diverse range of biological activities. Because of the potential pharmacological application, we established the transformation system to enhance the triterpenoid contents production.

• 한국환경복원기술학회지
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• 제18권1호
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• pp.83-89
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• 2015

Thalictrum species belongs to the Ranunculaceae and are perennial landscape plants which are available in the garden and potted plants. This experiment was conducted to find out the basic data for the growth effects of fertilizer levels and shading rate in seedling plug cell tray in the Thalictrum rochebrunianum, T. uchiyamai and T. coreanum. For T. rochebrunianum and T. uchiyamai, growth increased by all fertilizer level treatments as compared to non-treatment. Hyponex 1000 times of treatment showed the highest growth. All growth increased in 35% shading as compared to other treatments for T. rochebrunianum and T. coreanum. But, for T. uchiyamai growth increased in 55% shading. In general, growth of three Thalictrum species was worse in 75% shading, as compared to other shading rates. Based on the results, Thalictrum species seedling can be produced by 35-55% shading and fertilization of Hyponex 1000 times by using plug tray.

• 한국패류학회지
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• 제3권1호
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• pp.3-14
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• 1987

The electrophoretic and innunological cnalyses of organic matrices in the shells of freshwater bivalves were made in order to dlucidate the biochemical characteristics and species-specific differinces of the applied shells, The water-soluble and insoluble matrices of four species of freshwater bivalves, Andodonta fukudai, Unio douglasiae, Lanceolaria and Chrbicula fluminea, were used as analytical materials, There was non-identity in immuno affinity between anti soluble matrix(anti-Sm) and anti insoluble matrix(anti-ISM)sera against the organic matrix of Andodnta fukudai. The SMs of four species (S. fukudai, U.douglasiae, L. acrorhyncha, and C. fluminea) showed the differences in the precipitate arcs at the level of family, though ISMs did mot show differences. In the electrophoretic analysis, all foru species had two SDS-electrophoretic bands of SM, of which molecular wights appeared to be lower than 55,000, shereas the native organic matrices of foru speceis had higher molecular weighrs than those from SDS-dldctrophoresis. Only calcium ion among many ions in extrapallial fluid(EPF) caused SM to change into insoluble molecules, thus the EPF pretreated with Ca++did not form the precipitate arc when did the immuno diffusion whth anti SM serum. ISM precursor may be polymerized into macromolecules like periostracin, a precusor of periostracum, judging from the similat polymerization patterns in 0.1M Tris formate buffer(pH 3); they may be made insoluble macromolecules due to their strong natrue of hydrophobicity.

• ALGAE
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• 제34권2호
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• pp.91-97
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• 2019

Chilean species of marine macroalgae with amphitropical distributions oftentimes result from introductions out of the Northern Hemisphere. This possibility was investigated using haplotype data in an amphitropical red macroalgae present in Chile, Callophyllis variegata. Published sequence records from Canada and the United States were supplemented with new collections from Chile (April 2014-November 2015). Specimens of C. variegata were amplified for the 5′ end of the cytochrome c oxidase subunit I gene (COI-5P) and the full length nuclear internal transcribed spacer region. Haplotype networks and biogeographic distributions were used to infer whether C. variegata was introduced between hemispheres, and several population parameters were estimated using IMa2 analyses. C. variegata displayed a natural amphitropical distribution, with an isolation time of approximately 938 ka between hemispheres. It is hypothesized that contemporary populations of C. variegata were established from a refugial population during the late Pleistocene, and may have crossed the tropics via rafting on buoyant species of kelp or along deep-water refugia coincident with global cooling, representing a rare case of a non-human mediated amphitropical distribution.

• 한국자원식물학회지
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• 제13권3호
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• pp.202-207
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• 2000

자생식물 16종의 종자형질 특성 및 저온습윤 및 변온 처리에 의한 발아양상을 조사하기 위하여 본실험은 수행되었다. 종에 따라 종자의 길이, 넓이, 폭은 각각 1.1∼8.9, 0.7∼7.5, 0.4∼l.7mm의 범위였으며 천립중은 0.1∼8.8g의 범위였다. 무처리에서 발아율이 가장 높은 종에 있어 냉이와 익모초는 암조건에서 각각 16, 36%이었으며 장구채와 각시취는 명조건에서 각각 69, 81%이었다. 참취, 고려엉겅퀴, 곰취, 질경이, 청옥취는 저온습윤처리 명조건에서 각각 84, 29, 57, 78, 95%로 물엉겅퀴는 암조건에서 30%로 발아율이 가장 높았다. 변온처리에서 발아율이 높은 종에 있어 두릅나무와 벌개미취는 명조건에서 각각 2,57%로, 수리취는 암조건에서 52%로 가장 높았다.

• Journal of Ecology and Environment
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• 제46권1호
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• pp.1-7
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• 2022

Background: In this study, we proposed that the population dynamics of non-native red-eared sliders (Trachemys scripta elegans) depends on the species' habitat extension and survivorship. We used a logistic equation with time-dependent habitat carrying capacity. In detail, the present carrying capacity depends on the red-eared slider population of the previous year. Anthropogenic activities such as the abandonment of previously captive red-eared sliders or the release due to religion customs would supply new habitats to the species. Therefore we assumed that anthropogenic spread increases the habitat carrying capacity. Based on the urbanization increase rate of 3% in Korea from 1980 to 2000, we assumed an annual spread of 3% to simulate the population dynamics of the red-eared slider. In addition, the effect on the population of an increase of natural habitats due to migration was simulated. Results: The close relationship between the distributions of non-native red-eared sliders and of urbanized areas demonstrates that urbanization plays an important role in providing new habitats for released individuals. Depending on the survivorship, the population of the red-eared slider in Korea increased 1.826 to 3.577 times between 1980 and 2000. To control population growth, it is necessary to reduce carrying capacity by reducing habitat expansion through prohibition of release into the wild ecosystem and careful managements of the wetland or artificial ponds. Changes in the habitat carrying capacity showed that the population fluctuated every other year. However, after several years, it converged to a consistent value which depended on the survivorship. Further, our results showed that if red-eared sliders expand their habitat by natural migration, their population can increase to a greater number than when they have a 99% survivorship in a fixed habitat. Conclusions: Further introductions of red-eared sliders into wetlands or artificial ponds should be prohibited and managed to prevent future spread of the species. Moreover, it is important to reduce the species' survivorship by restoring disturbed ecosystems and maintaining healthy ecosystems.

• 원예과학기술지
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• 제32권4호
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• pp.550-557
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• 2014

Heloniopsis koreana and Heloniopsis tubiflora (Melanthiaceae) are endemic herbaceous species of the Korean Peninsula. The Melanthiaceae family has been described as having seeds with small, underdeveloped embryos at the time of dispersal, and morphological (MD) or morphophysiological dormancy (MPD). However, there are few reports on embryo growth, morphology, and seed germination in Heloniopsis species. The aims of this study were to investigate embryo growth and seed dormancy, and to determine the type of dormancy exhibited by these species. The effects of incubation temperatures, light conditions, and gibberellic acid ($GA_3$) on dormancy break and seed germination were tested. Freshly matured seeds of the two species had small embryos that occupied about 9-11% of the length of the endosperm, and which increased by more than 300% in length before radicle emergence, indicating that the embryos are underdeveloped at the time of dispersal. Embryos in the seeds grew under warm temperature regimes (between $25/15^{\circ}C$ and $30/20^{\circ}C$). $GA_3$ application (tested only in the light) overcame seed dormancy and promoted germination. Approximately 30% of the seeds of H. koreana and approximately 40% of the seeds of H. tubiflora germinated in suitable environmental conditions (light and temperature) within 4 weeks. Therefore, 30-40% of the seeds of the two species exhibited MD, and the rest of the seeds had non-deep simple MPD. Light was found to be one of the critical factors for germination because no seed of either of the two Heloniopsis species germinated under constant dark conditions, and thus, these species have the potential to form a persistent soil seed bank. Understanding these germination requirements will help in development of effective strategies to increase the establishment of seedlings in their native habitat.

• Current Research on Agriculture and Life Sciences
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• 제15권
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• pp.47-52
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• 1997

새우난초, 금새우난초 및 이들 교잡종자(交雜種子)의 기내(機內) 무균발아(無菌發芽)에 적합(適合)한 기내(機內) 배양조건(培養條件)을 구명(究明)하기 위하여 수분후(受粉後) 일수(日數), 기본배지(基本培地), 초음파(超音波) 처리효과(處理效果) 실험(實驗)을 수행(遂行)하였다. 수분후(受粉後) 100일(日)된 새우란 종자(種子)를 MS배지에 파종(播種)하였을 때 발아율(發芽率)이 가장 양호(良好)하였으며 초음파(超音波) 처리시간별(處理時間別)로는 10분간(分間) 처리(處理)한 다음 파종(播種)하였을 때 발아소요일수(發芽所要日數)는 단축(短縮)되고 발아후(發芽後) 유묘(幼苗)의 생육(生育)도 양호(良好)한 경향(傾向)이었다.

• 한국응용곤충학회지
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• 제52권3호
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• pp.255-259
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• 2013

온실에서 재배되는 수입 드라세나묘목 및 게발선인장에서 채집된 뿌리깍지벌레 2종 Ripersiella multiporifera Jansen 및 Rhizoecus albidus Goux를 우리나라에서 처음으로 보고한다. 이들은 수입된 식물을 통해 온실에 도입된 것으로 추정되며 우리나라에 추가적인 유입과 정착을 방지하기 위해 이들 종의 진단형질, 사진자료, 기주 및 분포정보를 제공하고자 한다.