• Title/Summary/Keyword: Non-ionic

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Extraction of β-glucosidase from Bagasse Fermented by Mixed Culture under Solid State Fermentation

  • Shata, Hoda Mohamed Abdel Halim;Farid, Mohamed Abdel Fattah
    • Journal of Applied Biological Chemistry
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    • v.57 no.3
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    • pp.197-203
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    • 2014
  • Various parameters such as solvent selection, concentration, solid/liquid ratio, soaking time, temperature, stationary, shaking conditions, and repeated extractions were investigated in order to determine the optimum extraction conditions of ${\beta}$-glucosidase from bagasse fermented by mixed culture of Aspergillus niger NRC 7A and Aspergillus oryzae NRRL 447. Among various solvents tested, non ionic detergents gave the best results than the inorganic or organic salt solutions and distilled water. The optimum conditions for extraction of ${\beta}$-glucosidase were 30 min soaking time at $40^{\circ}C$ under shaking condition at 150 rpm, with solid/liquid ratio 1:15 (w/v), which yielded $2882.74{\pm}95.52U/g$ fermented culture (g fc) of enzyme activity. With repeated washes under the above optimum conditions, the results showed that enzyme extracted in the $1^{st}$ and $2^{nd}$ washes represents about 90% of the total activity.

Inhibitory Effect of {Surfactant- MnO4-} Aggregation in KMnO4 Oxidation of Proline and Methionine: A Kinetic Study

  • Tripathi, Ritu;Upadhyay, Santosh K.
    • Journal of the Korean Chemical Society
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    • v.58 no.4
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    • pp.351-358
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    • 2014
  • Anionic (sodium lauryl sulphate, NaLS) cationic (cetyl ammonium bromide, CTAB) and non-ionic (Tween-80) surfactants have been found to inhibit the rate of oxiadation L-proline and L-methionine by alkaline $KMnO_4$. A first order dependence of rate of oxidation was observed with respect to $MnO_4{^-}$. The order of reaction in substrate and alkali was found to be fractional nearby 0.65 and 0.55 in Aminoacid and $OH^-$, respectively. An aggregation/association between $MnO_4{^-}$ and surfactant has been confirmed spectrophotometrically. A mechanism, involving kinetically inactive [$MnO_4{^-}$ surfactant] aggregate and consistent with kinetic data, has been proposed. The effect of surfactants has been discussed in terms of hydrophobic and electrostatic interactions.

Preparation and Optical Characterization of Mesoporous Silica Films with Different Pore Sizes

  • Bae, Jae-Young;Choi, Suk-Ho;Bae, Byeong-Soo
    • Bulletin of the Korean Chemical Society
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    • v.27 no.10
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    • pp.1562-1566
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    • 2006
  • Mesoporous silica films with three different pore sizes were prepared by using cationic surfactant, non-ionic surfactant, or triblock copolymer as structure directing agents with tetramethylorthosilicate as silica source in order to control the pore size and wall thickness. They were synthesized by an evaporation-induced self-assembly process and spin-coated on Si wafer. Mesoporous silica films with three different pore sizes of 2.9, 4.6, and 6.6 nm and wall thickness ranging from $\sim$1 to $\sim$3 nm were prepared by using three different surfactants. These materials were optically transparent mesoporous silica films and crack free when thickness was less than 1 m m. The photoluminescence spectra found in the visible range were peaked at higher energy for smaller pore and thinner wall sized materials, consistent with the quantum confinement effect within the nano-sized walls of the silica pores.

Spectrophotometric Determination of Nitrite Based on Its Reaction with p-Nitroaniline in the Presence of Diphenylamine in Micellar Media

  • Afkhami, Abbas;Masahi, Shokofeh;Bahram, Morteza
    • Bulletin of the Korean Chemical Society
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    • v.25 no.7
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    • pp.1009-1011
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    • 2004
  • In the present work a very simple, sensitive and selective spectrophotometric method for the determination of nitrite in micellar media is described. The method is based on the color reaction of nitrite with p-nitroaniline in the presence of diphenylamine in acid media. In order to remove the extraction step, Triton X-100, a non-ionic surfactant was used as micellar media. The optimum reaction conditions such as acid concentration, reagents concentration and effect of time have been studied and the analytical characteristics of the method such as limit of detection, linear range and molar absorptivity have been obtained. The interference of some anions and cations was also tested. The method was applied to the determination of nitrite in real samples.

Effects of the Presence of Nonionic surfactants on Diesel Biodegradation (비이온계 계면활성제가 미생물의 디이젤 분해에 미치는 영향)

  • Lee, Hyo-Sang;Jeong, Gi-Hyeong;Kim, Jeong-Rak;Lee, Gi-Se
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.424-425
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    • 2000
  • The effects of the presence of commercial non-ionic surfactants on the cell growth and diesel degradation by Pseudomonas sp. OSD were studied. Most surfactants inhibited the diesel biodegradation at high concentration(1000mg/1). However, some surfactants showed no inhibition at lower concentrations. Tween 20, Brij 58, Brij 78 were not inhibitory to the diesel biodegradation even at high concentration. These chosen surfactants has relatively high HLB values. There exists complicated relationship for diesel bioremediation between cell hydrophobicity, surfactant HLB, contaminants, an soil.

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Characteristics of High-Rate Filtration with Filtration Aids (급속여과공정에서의 여과보조제 사용에 따른 여과특성)

  • Ahn, Jong-Ho;Yoon, Jae-Heung
    • Journal of Korean Society of Water and Wastewater
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    • v.14 no.3
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    • pp.260-270
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    • 2000
  • The objective of this study is to evaluate the effect of filter aids in the dual-media rapid filtration. Paper-filter tests were conducted to determine the proper dosages of coagulant and filter aid, and pilot plant tests using two dual-media filter columns were performed for a variety of filtration rates. Using a filter aid (non-ionic polymer), the maximum feasible filtration rate is 480m/day, while turbidity is less than 0.3 NTU and filter run-time is about 70 hours. It is possible to increase the filtration rate up to 360 m/day for keeping the turbidity less than 0.1 NTU. Turbidity increases for the filtration rate greater than 360m/day. In general, the quality of filtered water with a filter aid is stable, while the filter maintains a sufficient filter run-time for a maximum allowable head loss. Particularly, the initial breakthrough can be effectively controlled. The use of a filter aid may be one of the methods applicable if the turbidity of filtered water is required to be improved or if the filter breakthrough limits filter run-time.

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The Application of Non-ionic Vat dye to Polyester Fiber : Practical Aspects and Preliminary Studies (비이온성 배트염료의 폴리에스터 섬유에의 응용)

  • 손영아;김태경
    • Textile Coloration and Finishing
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    • v.15 no.1
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    • pp.23-29
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    • 2003
  • Dyeings of the most widely used to the synthetic fibers, namely polyamide and polyester, have been carried out using acid dyes and disperse dyes. The above mentioned dye types, and indeed all dyes onto substrates, rely on the reaction properties between the substrates and dye molecules. In terms of fastness properties, however, especially to washings, the satisfactory levels are not present in the results from acid and disperse dyeings. Thus, vat dyeings leaves a feasibility and are an alternative way to overcome the problem. Although attempts and works have been reported in early years, with little if any commercial achievement and success. In this context, to meet increased demands towards a high level of wash fastness from consumers and retailers, an attempt using vat dyes has been carried out in this work.

Synthesis of Two-Component Titanate Powders Using Ethylene Glycol Solution (에틸렌글리콜 용액을 이용한 2성분계 Titanate 분말의 합성)

  • 이상진;권명도
    • Journal of Powder Materials
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    • v.9 no.5
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    • pp.346-351
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    • 2002
  • Pure and fine, two-component titanate powders (barium titanate, calcium titanate etc.) were synthesized by an ethylene glycol method. Titanium isopropoxide and other metal ionic salts were dissolved in liquid-type ethylene glycol without any precipitation. In non-aqueous system, the amount of ethylene glycol affected the solubility and homogeneity of metal cation sources in the solution. At the optimum amount of the polymer, the metal ions were dispersed effectively in solution and a homogeneous polymeric network was formed. Most of the synthesized powders had sub-micron or nano-size primary particles after calcination and the agglomerated calcined powders were easily ground by ball milling process. All synthesized titanate powders had stable crystallization behavior at low temperature and high specific surface area after ball milling. The crystallization behavior and the microstructures of the calcined powders were affected on the ethylene glycol content.

Synthesis of Alumina-Surfactant Mesophase with $Al_{l3}$-Keggin Cation ($Al_{l3}$ 거대 양이온으로부터 알루미나-계면활성제 중간상의 합성)

  • 김윤섭;고형신
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2000.10a
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    • pp.653-655
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    • 2000
  • Alumina-surfactant mesostructures have been synthesized with the $Al_{13}$-Keggin cation prepared by Al(N $O_3$)$_3$.9$H_2O$ solution with NaOH solution in the presence of a non-ionic surfactant at room temperature. The synthesized samples had the hexagonal structure similar to MCM-41 type materials. These samples have been characterized by X-ray diffraction and thermal analysis(TG). The samples prepared from OH/Al ratio 1.5 and 2.0 were well-crystalline mesostructures, but the sample from OH/Al ratio 2.5 was not. Also, The d$_{100}$ value decreased slightly from 38 to 36 according to the OH/Al mole ratio. These results could be explained that Keggin ion depended on the OH/Al molar ratio and pHpH

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Effect of Triton X-100 on Intracellular Accumulation of Cadmium in Hansenula anomala (카드뮴의 Hansenula anomala 세포내 축적에 미치는 Triton X-100의 효과)

  • 유대식;박정문;송형익
    • Korean Journal of Microbiology
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    • v.25 no.2
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    • pp.110-116
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    • 1987
  • As a pary of investgation on effective accumulation of cadmium in yeast cells, effect of surfactant was studied on intracellular accumulation of cadmium in extremely cadmium-tolerant yeast, Hansenula anomala B-7. Cadmium accumulation was enhanced up to approximately 40% by the addition of non-ionic surfactant, Triton X-100 and its optimal concentration was found to be 0.1-0.2%. Furthermore, optimum conditions for intracellular accumulation of cadmium were at $40^{\circ}C$ and initial pH 6.0 for 48 hours under shaking culture.

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