• Title/Summary/Keyword: Non-extraction

Search Result 1,065, Processing Time 0.024 seconds

Kinetic and Thermodynamic Analysis of AChE Inhibition of Solvent Extract Fractions from Inonotus obliquus (차가버섯 용매추출분획의 Acetylcholinesterase 저해활성에 대한 동역학 및 열역학적 해석)

  • Kim, Hak-Kyu;Hur, Won;Hong, Eok Kee;Lee, Shin-Young
    • Food Engineering Progress
    • /
    • v.15 no.4
    • /
    • pp.289-296
    • /
    • 2011
  • Twenty four fractions by solvent extraction and/or acid precipitation from fruit body and culture broth of Inonotus obliquus were prepared, and their inhibitory effect against acetylcholinesterase (AChE) was investigated. Among these fractions, acid (1 M HCl) precipitates from cell-free culture broth and fruit body exhibited the highest inhibitory effect on AChE in vitro. Acid precipitates inhibited AChE activity in a concentration-dependant manner and $IC_{50}$ values of both acid precipitates were 0.53 mg/mL. The inhibition pattern was general non-competitive inhibition. The energetic parameters were also determined by dual substrate/temperature design. Both acid precipitates increased the values of Ea, ${\Delta}H,/;{\Delta}G$ and ${\Delta}H^{\ast}$ decreasing the value of ${\Delta}S$ for AChE. The results implied that the acid precipitates from I. obliquus increased the thermodynamic barrier, leading to the breakdown of ES complex and the formation of products as inhibitory mechanism.

Polysaccharide Extraction and Comparison of Free Radical Scavenging Activities from Tremella fuciformis and Auricularia auricula Fruit Body (흰목이와 흑목이 버섯의 다당추출 및 유리라디칼 소거활성 비교)

  • Kim, Hyeon-Min;Hur, Won;Lee, Shin-Young
    • Food Engineering Progress
    • /
    • v.15 no.1
    • /
    • pp.6-14
    • /
    • 2011
  • The polysaccharides from fruit body of Auricularia auricula and Tremella fuciformis were extracted using hot water, and partially purified through ethanol precipitation and dialysis. Free radical scavenging activities of the crude and purified polysaccharides were examined and compared each other. Free radical scavenging activities of the partially purified polysaccharides were higher than those of crude polysaccharides. DPPH free radical, ABTS radical and SOD-like activities of partially purified polysaccharide at 1 mg/mL of concentration from A. auricula were 61.7, 9.6 and 38.9%, respectively, while those of T. fuciformis were 9.6, 5.7 and 15.3%, respectively. Results of site and non-site specific hydroxyl radical scavenging activities indicated that the partially purified polysaccharide fractions from A. auricula and T. fuciformis exhibited the hydroxyl radical scavenging effect by hydrogen donating ability and iron ion chelating ability. Also, reducing powers of A. auricula and T. fuciformis were 77.1 and 14.7% of BHT (0.1%) as standard, respectively. It was suggested that antioxidant activities of A. auricula were about 1.4~6.4 times higher than those of T. fuciformis due to different levels of polyphenol content.

Blind Waveform Estimation Scheme Based on ESPRIT for Nonuniform Linear Array MIMO Radars Using Distributed Multiple Electronic Sensors (분산 다중 전자전 센서를 이용한 ESPRIT 기반 비등간격 선형배열 MIMO 레이다의 암맹 직교신호 분리 기법)

  • Yeo, Kwanggoo;Chung, Wonzoo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.29 no.11
    • /
    • pp.891-897
    • /
    • 2018
  • In this paper, we propose a blind estimation scheme for the antenna spacing of nonuniform linear array MIMO radar using distributed electronic sensors based on ESPRIT. We present a blind method to separate orthogonal waveforms of a MIMO radar based on the antenna spacing estimation. The estimated orthogonal waveforms of a MIMO radar can be used for disabling opponent MIMO radars.

Influence of geometry and safety factor on fatigue damage predictions of a cantilever beam

  • Pecnik, Matija;Nagode, Marko;Seruga, Domen
    • Structural Engineering and Mechanics
    • /
    • v.70 no.1
    • /
    • pp.33-41
    • /
    • 2019
  • The influence of two parameters on fatigue damage predictions of a variably loaded cantilever beam has been examined. The first parameter is the geometry of the cantilever beam and the weld connecting it to a rear panel. Variables of the geometry examined here include the cantilever length, the weld width on the critical cross-section and the angle of the critical cross-section. The second parameter is the safety factor, as set out by the Eurocode 3 standard. An analytical approach has been used to calculate the stresses at the critical cross-section and standard rainflow counting has been used for the extraction of the load cycles from the load history. The results here suggest that a change in the width and angle of the critical cross-section has a non-linear impact on the fatigue damage. The results also show that the angle of the critical cross-section has the biggest influence on the fatigue damage and can cause the weld to withstand fatigue better. The second parameter, the safety factor, is shown to have a significant effect on the fatigue damage calculation, whereby a slight increase in the endurance safety factor can cause the calculated fatigue damage to increase considerably.

Long-term stability of maxillary and mandibular arch dimensions when using rapid palatal expansion and edgewise mechanotherapy in growing patients

  • Kim, Ki Beom;Doyle, Renee E.;Araujo, Eustaquio A.;Behrents, Rolf G.;Oliver, Donald R.;Thiesen, Guilherme
    • The korean journal of orthodontics
    • /
    • v.49 no.2
    • /
    • pp.89-96
    • /
    • 2019
  • Objective: The purpose of this study was to assess the long-term stability of rapid palatal expansion (RPE) followed by full fixed edgewise appliances. Methods: This study included 67 patients treated using Haas-type RPE and non-extraction edgewise appliance therapy at a single orthodontic practice. Serial dental casts were obtained at three different time points: pretreatment ($T_1$), after expansion and fixed appliance therapy ($T_2$), and at long-term recall ($T_3$). The mean duration of the $T_1-T_2$ and $T_2-T_3$ periods was $4.8{\pm}3.5years$ and $11.0{\pm}5.4years$, respectively. The dental casts were digitized, and the computed measurements were compared with untreated reference data. Results: The majority of treatment-related increases in the maxillary and mandibular arch measurements were statistically significant (p < 0.05) and greater than expected for the untreated controls. Although many measurements decreased postretention ($T_2-T_3$), the net gains persisted for all of the measurements evaluated. Conclusions: The use of RPE therapy followed by full fixed edgewise appliances is an effective method for increasing maxillary and mandibular arch width dimensions in growing patients.

Imaging and analysis of genetically encoded calcium indicators linking neural circuits and behaviors

  • Oh, Jihae;Lee, Chiwoo;Kaang, Bong-Kiun
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.23 no.4
    • /
    • pp.237-249
    • /
    • 2019
  • Confirming the direct link between neural circuit activity and animal behavior has been a principal aim of neuroscience. The genetically encoded calcium indicator (GECI), which binds to calcium ions and emits fluorescence visualizing intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes. These GECI-based signals can be acquired by several tools including two-photon microscopy and microendoscopy for precise or wide imaging at cellular to synaptic levels. In addition, the images from GECI signals can be analyzed with open source codes including constrained non-negative matrix factorization for endoscopy data (CNMF_E) and miniscope 1-photon-based calcium imaging signal extraction pipeline (MIN1PIPE), and considering parameters of the imaged brain regions (e.g., diameter or shape of soma or the resolution of recorded images), the real-time activity of each cell can be acquired and linked with animal behaviors. As a result, GECI signal analysis can be a powerful tool for revealing the functions of neuronal circuits related to specific behaviors.

A Multi-Indexes Based Technique for Resolving Collision in a Hash Table

  • Yusuf, Ahmed Dalhatu;Abdullahi, Saleh;Boukar, Moussa Mahamat;Yusuf, Salisu Ibrahim
    • International Journal of Computer Science & Network Security
    • /
    • v.21 no.9
    • /
    • pp.339-345
    • /
    • 2021
  • The rapid development of various applications in networking system, business, medical, education, and other domains that use basic data access operations such as insert, edit, delete and search makes data structure venerable and crucial in providing an efficient method for day to day operations of those numerous applications. One of the major problems of those applications is achieving constant time to search a key from a collection. A number of different methods which attempt to achieve that have been discovered by researchers over the years with different performance behaviors. This work evaluated these methods, and found out that almost all the existing methods have non-constant time for adding and searching a key. In this work, we designed a multi-indexes hashing algorithm that handles a collision in a hash table T efficiently and achieved constant time O(1) for searching and adding a key. Our method employed two-level of hashing which uses pattern extraction h1(key) and h2(key). The second hash function h2(key) is use for handling collision in T. Here, we eliminated the wasted slots in the search space T which is another problem associated with the existing methods.

Determination of Mono-, Di-, and Tri-Butyltin Compounds in Seawater of Several Bays in Korea

  • Choi, Hwa-Seon;Kwon, Ee-Yol;Lee, Dong-Sup
    • Bulletin of the Korean Chemical Society
    • /
    • v.14 no.2
    • /
    • pp.234-238
    • /
    • 1993
  • The method of study describes the determination of the nanogram amounts of mono-, di-, and tri-butyltin compounds in sea water. The procedure is based on 1) the conversion of tin compounds to non-ionic species by sodium tetraethyl borate as an alkylation reagent, 2) one-step ethylation and 3) extraction. No further purification or concentration of the extract was required before GC-FPD (Flame Photometric Detector). The detection limits for mono-, di- and tri-butyltin were 12.50, 6.02, and 4.19 ng/L. The linear range (0-120 ng/L) was solely dependent on the detector response height. Sea water samples (n=24) were obtained from Masan Bay, Busan, Ullsan, Gunsan, Sockcho, Yeasu, Donghae, Inchun, Kanghwa, and Ahsan in Korea. The samples were collected between June, 1992 to July, 1992 and the distribution of monobutyltintrichloride (MBTC), dibutyltindichloride (DBTC), and tributyltinchloride (TBTC) in sea water was recorded. The concentrations of butyltin compounds in seawater were found to be in the range of N.D. (not detected)-61.93 ng/L for MBTC, N.D.-32.16 ng/L for DBTC, and N.D.-55.76 ng/L for TBTC.

Nitric Oxide Production and Acetylcholinesterase Inhibitory of Activity Various Extracts from Codonopsis lanceolata by Steaming Times (증숙 더덕 용매별 추출물의 Nitric Oxide 생성 저해 효과 및 Acetylcholinesterase 저해활성)

  • Choi, Hyun-Suk;Choi, DuBok
    • The Korean Journal of Food And Nutrition
    • /
    • v.34 no.3
    • /
    • pp.295-301
    • /
    • 2021
  • Steaming is a method that has traditionally been used for medicinal plant extraction. This study investigated nitrite oxide production, ferrous ion chelating activity, α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities of ethanol, acetone and hot-water extracts of Codonopsis lanceolata prepared by steaming seven times. MTT assay showed that each extract was non-toxic up to a concentration of 700 ㎍/mL confirming that there was no cytotoxicity in all extracts. The α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities exhibited by the hot-water extract obtained from steaming seven times were higher (83.1%) than the other extracts. Higher production of nitrite oxide and better ferrous chelating activity was recorded with hot-water extract compared to ethanol and acetone extracts. These results indicated that more steaming of Codonopsis lanceolata extracts would be required to validate the possibility of developing antioxidants. Also, further study is needed to determine if the components present in the tested extracts might be useful in the prevention of Alzheimer's disease. These results showed that hot-water extracts may be useful for their antioxidant and the production inhibitory activity of nitrite oxide. It will be helpful in the investigation of the constituent analysis of the steam-processed product of Codonopsis lanceolata.

Tree image comparison analysis using LBP method (LBP 방식을 이용한 나무 영상 비교 분석)

  • Kim, Ji-hong;Lee, Jonghyun
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.25 no.4
    • /
    • pp.530-536
    • /
    • 2021
  • Since the LBP algorithm has the characteristic of local texture expression, it is possible to obtain completely different results depending on the extraction location and the size of the reference image and the sample image. In order to solve these shortcomings, in this paper, we first investigate the basic characteristics of LBP, make the size of the reference image (100×100) in order to include most of the characteristics in the image, and select a sample image (40×40) extracted from an arbitrary point. After finding the matching position in the LBP of the reference image by using the correlation test between the LBP of the reference image and the LBP of the sample image, a chi analysis method is used to find the reference image that most closely matches the sample image.